Objective To investigate the clinical application effect of SPIDER limbs positioning device in arthroscopic surgery of beach chair position.Methods From August 2009 to August 2010 in our hospital 18 cases of patients with rotator cuff injury in shoulder were collected and treated with arthroscopic exploration and suturing operation,including 9 patients in the standard beach chair position under surgical operation,9 cases of beach chairs position combine with SPIDER Limb positioning device.Follow up on the preoperative position placement time,operating time,doctors' satisfaction of surgery posture and postoperative complications.Results The preoperative placement position time of standard beach chair with a mean (27 ± 6.7 ) min,beach chair position combined with SPIDER limb positioning device with a mean (31 ±7.9)min,the two groups were no significant differences ( t =- 1.158,P ＞ 0.05 ) ; the operation time of two groups were ( 78.2 ± 12.7 ) min and (66.2 ± 15.4) min,beach chair position combined with SPIDER limb positioning device group compared with the former was short,there was a between the two groups significant difference; doctors' surgery position satisfaction were 77.8％ and 88.9％ respectively,and there was no complications occurred related with posture.Conclusions SPIDER limb positioning device in the beach chair position in shoulder arthroscopy allows surgeon change positions of limbs during the operation in the sterile area.It has high satisfaction with the surgical posture,practicability,advanced and worthy of clinical application.

Neural stem cells in brains have capacities of proliferation and differentiation, which is very critical to rebuild the cerebral cortex functions. Therefore, it is of great importance to find key targets and network pathways that regulate the proliferation of neural stem cells, which is also a pressing problem in the medical circle. With the Notch pathway as the core of the network, this paper summarized the advance of the bimolecular network system composed of Wnt, Shh, EGFR, cytokines and Notch signal, and analyzed such key nodes as Notch receptor, CBF1, NICD, Hesl, which may become potential targets of new-type drugs in the future. With the multi-component, multi-target, multi-lever characteristics, traditional Chinese medicines have many common grounds with the network pharmacology. The active component groups or active ingredients in traditional Chinese medicines are one of the material bases for showing their network pharmacological effect, which is worth exploring. This paper aims to provide a new strategy for the treatment of neurodegenerative disease and nerve injury with traditional Chinese medicines.
Animals ; Cell Proliferation ; Humans ; Neural Stem Cells ; cytology ; metabolism ; Signal Transduction ; Systems Biology

Aged ; Case-Control Studies ; Cerebral Infarction ; blood ; genetics ; Female ; Fibrinogen ; genetics ; metabolism ; Haplotypes ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic

AIMTo explore the effects of sleep deprivation (SD) on myocardium and its antioxygen index.

METHODS35 Sprague Dawley rats were randomly divided into five groups: Cage control, Tank control, SD 2 d, SD 4 d and SD 6 d. The "flower pot" technique was used to establish rats sleep deprivation model followed by record of surface electrocardiogram, detection of myocardium morphology changes under microscope and transmission electron microscope and investigation of MDA content and SOD activity of myocardial mitochondria.

RESULTSAfter sleep deprivation, heart rate increased and ECG showed ischemia of myocardium; subcellular organelles such as chromosome, endoplasmic reticulum, mitochondria, intercalated disk impaired, myofibril lysis or necrosis, and lipid peroxidation reaction effects spread widely; edema, bleeding of the microvessels and invasion of the monocytes could be seen in the lumen. The MDA level increased and SOD activity increased followed by a decreased trend.

CONCLUSIONSleep deprivation can induce damage on myocardium, and the stress especially oxygen stress caused by SD may be the possible mechanism.

Animals ; Electrocardiography ; Female ; Male ; Myocardial Ischemia ; etiology ; pathology ; physiopathology ; Oxidative Stress ; physiology ; Rats ; Rats, Sprague-Dawley ; Sleep Deprivation ; complications ; Superoxide Dismutase ; metabolism

Objective: To observe the effects of acupoint thread-embedding therapy on serum apelin and glucagon-like peptide-1 (GLP-1) in type 2 diabetes mellitus patients with obesity due to dampness-heat encumbering spleen.Methods: Sixty-six patients were randomly divided into a control group and an observation group according to the random number table method, with 33 cases in each group. Patients in the control group were treated with exenatide and metformin, while patients in the observation group were treated with additional acupoint thread-embedding. After 12-week treatment, the obesity-related indicators, including body mass index (BMI), waist circumference and body fat rate, the glycometabolism indicators, including fasting blood glucose, 2 h postprandial blood glucose and glycosylated hemoglobin, and the lipid metabolism indicators, including total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), as well as serum apelin and GLP-1 levels were observed in patients of the two groups. Results: After treatment, the BMI, waist circumference and body fat rate of patients in the two groups were all reduced (all P<0.05), and were lower in the observation group than in the control group (all P<0.05); the fasting blood glucose, 2 h postprandial blood glucose and glycosylated hemoglobin levels of patients in both groups were all decreased (all P<0.05), and were significantly lower in the observation group than in the control group (all P<0.05); the TC level was decreased (P<0.05), while the TG and LDL-C levels did not change significantly in the control group (both P>0.05); the TC, TG and LDL-C levels were all significantly reduced in the observation group (all P<0.05), lower than those in the control group (all P<0.05); the serum apelin level was decreased (P<0.05) and the serum GLP-1 level was increased (P<0.05) in the observation group, statistically different from those in the control group (both P<0.05). Conclusion: Combined with the conventional medication, acupoint thread-embedding therapy can significantly improve the obesity-related indicators, glycometabolism and lipid metabolism in type 2 diabetes mellitus patients with obesity due to dampness-heat encumbering spleen. This may be achieved by regulating the serum apelin and GLP-1 levels.

Objective: To analyze the epidemiological characteristics of influenza clusters in Hangzhou from 2018 to 2019, so as to provide references for the prevention and control of influenza. Methods: The data came from the epidemic investigation reports of influenza clusters in Hangzhou from the 27th week of 2018 and the 26th week of 2019. The time distribution, school types, population distribution and etiology of influenza were analyzed. Multivariate logistic regression model was employed to analyze the influencing factors for the attack rate of influenza clusters. Results: During the surveillance season, a total of 231 school influenza clusters involving 4 233 cases were reported. The median of the attack rate was 21.74%. The peak of the clusters was in March 2019, with 89 events and 1 476 cases ( 34.87% ). The clusters occurred mainly in primary schools ( 188 events, 81.39% ) and were mainly caused by Victoria-like strains of influenza B virus ( 84 events, 36.36% ). The multivariate logistic regression analysis indicated that infection of teachers increased the risk of high attack rate ( OR=3.133, 95%CI: 1.180-8.320 ), and kindergartens had higher risk of high attack rate than primary schools ( OR=4.123, 95%CI: 1.579-10.763 ). Conclusions The influenza clusters in Hangzhou from 2018 to 2019 is mainly caused by Victoria-like strains of influenza B virus. Kindergartens and teachers are the key points for the prevention and control of influenza clusters.

Objective To explore a proper management model in operating room (OR) nursing training program in general hospital,and improve teaching quality and train nurse specialists.Methods Building a professional training team for OR nurses.According to hospital' s own condition,train OR nurses working in all clinical fields.Results We have achieved a new management model and improved training system with a high level training team and have successfully done the training job and got good results.Conclusions Building up a professional training team is beneficial for training qualified personnel and improving the development of nursing in the OR.

OBJECTIVETo observe the influence of H102 on the expression of amyloid protein and amyloid precursor protein in the hippocampus of APP695 transgenic mice.

METHODSThe 9-month-old APP695 transgenic mice were randomly divided into the model group and the H102 group; C57BL/6J mice were adopted as normal control group. The H102 group were injected with H102 in a dose of 3 microl/per mouse in lateral ventricle, once a day, for ten days; while the model group and the control group were injected with saline. The hippocampus and temporal cortex of the brain sections from transgenic mice and wild type female mice were subjected to immunohistochemistry and Congo red histological staining, and observed the difference of the protein expression under microscope. The expression of the APP protein was detected by Western blot.

RESULTSAbeta and APP immunohistochemistry showed density of positive cell in the CA1 region of hippocampus of control group were less than model group. H102 peptide reduced the area, and density of positive cells. Congo red staining showed there were lots of amyloid plagues in the brains of model mice but not in the brains of normal control. And the Western blot showed the content of the APP protein of the model group was much higher than the H102 group. H102 significantly decreased the amyloid plagues.

CONCLUSIONThe expression of APP, Abeta are increased in APP695 transgenic mice, and H102 can decrease the level of APP, Abeta in transgenic mice.

Amyloid beta-Protein Precursor ; metabolism ; Amyloidogenic Proteins ; metabolism ; Animals ; Brain ; metabolism ; Female ; Gene Expression ; Hippocampus ; metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic

OBJECTIVETo investigate the relationship between copper speciation and microbial features (microbial communities and copper tolerance level) in order to determine the adverse effect of different forms of Cu on microorganisms.

METHODSTessier's sequential extraction procedure was used to qualify the different Cu forms (exchangeable, carbonate bound, Fe/Mn oxide bound, residue and organic matter bound), and the copper tolerance level (expressed as IC50, influence concentration) was measured by the plate-count method.

RESULTSBy simple correlation analysis, the IC50 was positively correlated with the concentration of exchangeable Cu (R2 = 0.8204), while weakly correlated with other forms of Cu.

CONCLUSIONThe bacterial community tolerance increases in the copper-contaminated soil while sensitive bacteria decrease in the copper-contaminated soils. The exchangeable Cu exerts high toxicity to microbial communities.

Bacteria ; drug effects ; isolation & purification ; China ; Copper ; analysis ; chemistry ; toxicity ; Environmental Monitoring ; Fungi ; drug effects ; isolation & purification ; Hydrogen-Ion Concentration ; Soil Microbiology ; Soil Pollutants ; chemistry ; toxicity

Objective To explore the management of new nurse in large operation room,and further to improve the teaching quality of nursing in operation room to train specialist in nursing.Methods The managements of new nurses were including overall basic teaching,special course and overall evaluation management model.The training methods were including creating a operation room teaching team and whole range advisor in combination with one-to-one teaching.Results One hundred and eight nurses from 2008 to 2011 passed the check and accomplished the role change smoothly.The teaching management was constructed,and the teaching effect was very good.Conclusions It is very important to select management mode according to the clinical need,which fits the rapid development of operation room and trains specialist in nursing for clinic in time.