OBJECTIVETo study the volatile regularity of menthol and borneol in granule and lozenge at different temperature.

METHODKinetic method with GC being detection technique.

RESULTThe volatility of menthol and borneol acts as a pseudo first-order reaction in open system. Under the same condition, the volatile rate of menthol and borneol in granule is four times as fast as that in lozenge, and the volatile rate of borneol is faster than that of menthol.

CONCLUSIONThis study can be applied to improve the quality of lozenges containing menthol or/and borneol.

Bornanes ; administration & dosage ; chemistry ; Cyclodextrins ; Drug Carriers ; Drug Stability ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Menthol ; administration & dosage ; chemistry ; Tablets ; Temperature ; Volatilization ; beta-Cyclodextrins

OBJECTIVETo explore the prognostic impact of bone marrow involvement (BMI) and therapy in diffuse large B cell lymphoma (DLBCL).

METHODSThe clinical characteristics and prognosis of 83 DLBCL patients with or without BMI were retrospectively analyzed. The treatment outcome of standard CHOP regimen (CHOP group), intensive-dose regimen (intensive-dose group) and rituximab combined therapy (rituximab group) were compared.

RESULTSThe adverse prognostic factors including LDH elevation, ECOG score > or =2, higher IPI and aaIPI score, B symptom, hepatomegaly, splenomegaly, hemoglobin <110 g/L, platelet <100 x 10(9)/L and serum albumin <35 g/L were more prevalent in DLBCL patients with BMI than in those without BMI. Multivariate analysis showed that BMI was an independent prognostic factor of DLBCL. The 3-year OS and PFS rates in rituximab group were 78.1% and 64.3%, respectively, being statistically higher than that in CHOP group (23.6% and 21.8% respectively, P = 0.000 for both) and in intensive-dose group (33.3% and 25.7% respectively, P = 0.002 and 0.001, respectively). But no difference between the latter two groups (P = 0.411 and 0.694, respectively). For the patients with BMI, the 3-years OS and PFS in rituximab group (57.1% and 57.1%) were statistically higher than that in CHOP group (13.9% and 14.1%) and intensive-dose group (29.5% and 16.8%) (P = 0.029 and 0.012 respectively), respectively and also no difference in the latter two groups (P = 0.226 and 0.376 respectively). In the rituximab group, the 3-years OS and PFS were 86.7% and 67.3% respectively in patients without BMI, being higher than that in patients with BMI (57.1% and 57.1%), but the difference was not statistically significant (P = 0.645 and 0.965 respectively).

CONCLUSIONBMI is a negative independent prognostic factors of DLBCL patients. The rituximab combined chemotherapy can significantly improve the therapeutic effect of the DLBCL, and relieve the negative impact of BMI.

Adolescent ; Adult ; Aged ; Antibodies, Monoclonal, Murine-Derived ; administration & dosage ; Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; therapeutic use ; Bone Marrow ; pathology ; Child ; Child, Preschool ; Cyclophosphamide ; administration & dosage ; therapeutic use ; Doxorubicin ; administration & dosage ; therapeutic use ; Female ; Humans ; Lymphoma, Large B-Cell, Diffuse ; drug therapy ; pathology ; Male ; Middle Aged ; Neoplasm Invasiveness ; Prednisone ; administration & dosage ; therapeutic use ; Prognosis ; Retrospective Studies ; Rituximab ; Treatment Outcome ; Vincristine ; administration & dosage ; therapeutic use ; Young Adult

OBJECTIVETo investigate the clinical value of 64-slice computed tomographic angiography (CTA)-based virtual colonoscopy in the diagnosis of colonic tumors.

METHODSPhilips/Brilliance 64 CT volumetric scanning was performed in 8 patients with colonic cancer and 2 with colonic polypi identified by postoperative pathological examination. Mimics software was used for surface rendering of the intestine with the Marching Cubes algorithm for 3-dimensional (3D) virtual endoscope (VE) reconstruction and CTA-based 3D reconstruction of the large intestine and the surrounding structures. The location, volume and appearance of the lesions displayed by the virtual techniques were compared with the pathological results.

RESULTSThe 3D reconstruction was successfully completed in all the 10 cases, and the imaging diagnoses showed a total match with the pathological diagnoses. No significant differences were found between virtual endoscopy and CT virtual endoscopy. Virtual colonoscopy combined with digital model reconstruction provided valuable information for accurate identification of the position of the lesions and the complex adjacent anatomical structures.

CONCLUSIONVirtual colonoscopy based on 64-slice CTA, when combined with 3D reconstruction technique, allows accurate display of the colonic lesions and potential metastasis, which can be crucial for clinical staging and surgical planning of colonic cancer.

Adult ; Angiography ; methods ; Colorectal Neoplasms ; diagnostic imaging ; therapy ; Female ; Humans ; Image Processing, Computer-Assisted ; methods ; Imaging, Three-Dimensional ; Male ; Middle Aged ; Tomography, Spiral Computed

OBJECTIVETo explore the mechanism of 'erythroblast island (EI)' formation in the bone marrow of patients with immun-related hemocytopenia (IRP).

METHODSThe category of BM-auto antibody (au Ab) in 48 patients with IRP was detected with FCM. The BM-au Ab in the 'EI' of these cases were explored with immuonhistofluorescence (IF). Clinical and laboratory characteristics of these cases were also analyzed retrospectively.

RESULTSIgG could be detected in the 'EI' on the BM smear of 14 cases (29.17%), BM-au Ab mainly deposited at the edge/membranes between macrophage and erythroblasts rather than cyto plasm. Positive reaction were seen in all the cases with GlycoAIgG. The red blood cell count [(1.8 ± 0.5) × 10(12)/L] and hemoglobin level [(59.6 ± 16.2)g/L] were significantly lower than that in the IF(-) group [(2.5 ± 0.9) × 10(12)/L and (83.4 ± 25.0) g/L] (P < 0.05). The percentage of reticulocyte [(2.0 ± 0.8)%], serum level of IBIL [(9.4 ± 4.7) µmol/L], percentage of erythroblats in sternum BM (0.441 ± 0.139) and response rate to therapy (85.7%) in IF(+) group were significantly higher than that in IF(-)group [(1.3 ± 1.0)%, (6.6 ± 6.7)µmol/L, 0.298 ± 0.082, 61.3%, respectively] (P < 0.05).

CONCLUSIONMacrophage was connected with erythroblasts through autologous IgG in the 'EI's of some patients with IRP. 'EI' were the places where macrophages devoured and destroyed erythroblasts rather than erythroid development and differentiation. The pathogenetic mechanism of IRP might be associated with macrophages phagocytosing and destroying BM hematopoietic cells.

Blood Cell Count ; Bone Marrow ; Bone Marrow Cells ; immunology ; Coombs Test ; Erythroblasts ; Humans

Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Leukemia, Prolymphocytic, B-Cell ; diagnosis ; Male ; Middle Aged ; Retrospective Studies

Background: and Purpose Mutations in the FIG4 gene have been linked to amyotrophic lateral sclerosis (ALS) type 11 in Caucasian populations. The purpose of this study was to identify FIG4 variants in a cohort of 15 familial ALS (FALS) indexes and 275 sporadic ALS (SALS) patients of Han Chinese origin. Methods: All 23 exons of FIG4 were sequenced using targeted next-generation sequencing.An extensive literature review was performed to detect genotype-phenotype associations of FIG4 mutations. Results: No FIG4 variants were identified in the FALS patients. One novel heterozygous missense variant (c.352G>T [p.D118Y]) and one novel heterozygous nonsense variant (c.2158G>T [p.E720X]) in FIG4 were identified in two SALS patients. The p.E720X variant is interpreted as likely pathogenic while the p.D118Y variant is a variant of uncertain significance. The patient carrying the p.E720X mutation developed lower-limb-onset slowly progressive ALS, and survived for 11.5 years. The patient harboring the FIG4 p.D118Y variant also presented with progressive ALS, with the score on the ALS Functional Rating Scale–Revised (ALSFRS-R) decreasing by 0.4 per month. The rate of decrease in the ALSFRS-R scores from symptom onset to diagnosis seemed to be lower in the patients carrying FIG4 variants than the no-FIG4-mutation ALS patients in this study. Conclusions Our findings suggest that ALS patients carrying FIG4 mutations are not common in the Chinese population and are more likely to exhibit slow progression.

Objective To study the correlation between health behavior and social support of patients with chronic obstructive pulmonary disease (COPD).Methods Convenience sampling method was used to select 69 cases of COPD in a Class 3 hospital in Guangzhou who were stable enough to be permitted for discharge and ambulatory treatment.General information questionnaire,health promotion lifestyle (HPL) and social support research scale (SSRS) were used to assess their health behavior and social support.Results Total score of the 69 COPD patients' social support was (30.580 ± 5.097),which was medium.And total score of their health behavior was (130.710 ± 16.348),in which scores of health responsibility,body activity,nutrition,metal health,interpersonal relationship and stress adjustment were all lower than 33％,which was thelow level.There was statistically significant difference between every dimension of social support and dimensionsin health behavior including health responsibility,body activity,nutrition,metal health and stress adjustment(r =0.239-0.533,P ＜ 0.05).There was no statistically significant difference between objective support,support availability in social support and interpersonal relationship in health behavior (P ＞ 0.05).The total score of COPD patients' health behavior was related to the total score of their social support (r =0.371,P ＜0.01).Conclusions The health behavior and social support of COPD patients is positively correlated.Better social support and targeted nursing intervention can improve their health behavior.It is,therefore,an effective way to improve COPD patients' heath behavior by raising the level of social support.

Objective To research the sequencing weaning in the patients with chronic obstructive pulmonary disease (COPD). Methods The COPD patients were divided into two groups in this prospective case-control study: protocol-directed group vs none- protocol-directed group. The patients in the former group received programmed ventilator weaning strategy, detaily patients were selected through the shallow breathing with fast exponential screening, then were conducted by spontaneous breathing trial SBT , finally were pulled out the endotracheal intubation. The patients in the latter group were determined the timing of ending the ventilator according to doctors subjective judgment and clinical experience. Results The ventilation time of protocoldirected and on-protocol-directed group was ( 8.01 ± 7. 22 ) and ( 10.74 ± 5.58 ) days ( P = 0. 034 ) respectively. ICU stay time was shorter in protocol-directed group than in non-protocol-directed group ( 8.86 ±6.92 vs 12 ±6.10 days, P =0. 026). The success rate of weaning in protocol-directed group was 76.31% (29/38),which was higher than that in non-protocol ～ directed group( 12/25,48% , P = 0. 009 ). Conclusions Protocoldirected weaning and SBT may reduce the ventilation time and ICU stay time and enhance success rate of weaning.

In the present investigation, antagonistic action of cholecystokinin octapeptide (CCK-8)against morphine on the electrical and contractile activity of rat jejunum in vitro was studied. The results showed that the potentiation of acetylcholine (ACh) on both the burst of spike and the contractility were inhibited by morphine, which could be completely antagonized by CCK-8. The CCK-8 effect, again, could be suppressed by CCK-A receptor antagonist devazepide ( 10 nmol/L), but partially by CCK-B receptor antagonist L-365,260 at 10 nmol/L or completely at concentration of 30 nmol/L. The above results demonstrated that the antagonism of CCK-8 on morphine was mediated by both CCK-A and CCK-B receptors.

Objective To study the expressional pattern of bone morphogenetic protein-2 (BMP-2) in rostral neural stem cells migratory stream at different time in developing rat brain. Methods To examine the expression of BMP-2 by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemisyry. Results BMP-2 were expressed in all the SVZa, RMS and OB areas in rat brain at different developmental stages. The expression level of BMP-2 mRNA is not very high. (1) The expression level of BMP-2 were obvious highest at the E14 than at the P0 and P7, and there are lowest at P7 at the same location; (2) the expression level of BMP-2 were higher at OB and SVZa than at RMS at same developing stage. Conclusion The spatial-temporal expressional pattern of BMP-2 in SVZa, RMS and OB of rat brain indicates that BMP-2 maybe is an important factor regulating the proliferation, migration and differentiation of NSCs derived from SVZa.