Cervical Intraepithelial Neoplasia ; diagnosis ; pathology ; surgery ; Colposcopy ; Conization ; Electrosurgery ; Female ; Humans ; Hysterectomy ; methods ; Neoplasm Regression, Spontaneous ; Uterine Cervical Neoplasms ; diagnosis ; pathology ; surgery ; Vaginal Smears

ObjectiveTo investigate the role of vascular endotheliar growth factor(VEGF),chemokine receptor 4(CXCR4) and stromal cell-derived factor-1(SDF-1) in Wilms tumor as well as their relationship with clinical features by examining the expressions of VEGF,CXCR4 and SDF-1.MethodsThirty cases of Wilms tumor samples and 12 cases of adjacent kidney tissue were collected from the First and the Third Affiliated Hospital of Zhengzhou University from May 2003 to May 2008.Thirteen boys and 17 girls aging from 4 months to 7 years old of whom were 22 favorable histologic types and 8 unfavorable histologic types.By means of cytoplasm to dye buffy for masculine cell,staining intensity and percentage of positive tumor cells serve as the judgment criteria for being positive or negative.All tissues would be tested by hematoxylin and eosin stain at the same time.ResultsThe positive expression rate of VEGF,CXCR4 and SDF-1 in Wilms tumor were 63.33%,70.0% and 53.33%.Those in adjacent normal kidney tissue were 25.0%,8.3% and 16.7%.The 2 groups were statistically significant(Pa

Objective To investigate the clinical features of spinal muscular atrophy type Ⅱ.Methods The data of clinical manifestation,laboratory data, onset, diagnosis, and rehabilitation of 53 outpatients suffering from spinal muscular atrophy type Ⅱ (SMA-Ⅱ) were analyzed. Results Among 53 patients with SMA-Ⅱ confirmed by molecular genetic tests, 27 patients were male while 26 were female. The mean age was 3.35 years (ranged 0.75~7.8 years), and the age receiving gene diagnosis was 17.27 months. Only 15% had a family history,and abnormalities were found in 23% patients' mothers during pregnancy. 83.4% of them had water choke cough, while 87% expectoration weakness. None of them had cough assist machines and had regular spirometry monitoring. 53% of the patients took semi-liquid diet, however,none of them used stomach tube. Symmetrical flaccid paralysis was so remarkable, even muscle strength of lower limbs in 87.7% of these patients were only grades 1-2. 92% had scoliosis, while 83% had tendon contracture. EMG showed extensive neurogenic changes. All children did not accept normal pre-school education, and 85% patients did not accept formal rehabilitation. Conclusion Most of phenotype of SMA-Ⅱ for the children was similar and more severe in China. Most of the patient didn't get formal education, rehabilitation and care.

Child ; Female ; Humans ; Muscular Dystrophies ; complications ; congenital ; Spondylitis, Ankylosing ; complications

Adolescent ; Child ; Child, Preschool ; Female ; Gastroesophageal Reflux ; complications ; Humans ; Infant ; Male ; Retrospective Studies ; Superior Mesenteric Artery Syndrome ; complications ; Vomiting ; etiology

AIM:To observe the effects of Dickkopf-3 ( Dkk-3 ) in diabetic retinopathy ( DR ) circulating blood in patients with the expression level, the Dkk - 3 development changes in the diabetic retinopathy of significance in the diagnosis of early DR.
● METHODS: Eighty - five type 2 diabetic patients, included the non - proliferative diabetic retinopathy ( NPDR ) 23 patients, proliferative diabetic retinopathy, proliferative DR ( PDR ) in patients with 30 and non-diabetic retinopathy ( NDR ) with 32 cases. The same period of healthy physical examination was selected as control group ( 80 cases ) . Serum samples were collected, and the relative expression level of Dkk-3 was detected by enzyme - linked immunosorbent assay ( ELlSA) double antibody sandwich assay. The statistical differences were compared between groups.
●RESULTS: The plasma level of Dkk - 3 ( 430. 16 ± 198. 11pg/mL) in DR patients was significantly lower than that in healthy control group (627. 48±294. 45 pg/mL; P<0. 05 ) and NDR patients ( 601. 99 ± 194. 16 pg/mL; P<0. 05). While there was no significant difference in Dkk-3 level between NDR and healthy control group ( P =0. 729). The level of PDR in patients with Dkk-3 (396. 38± 185. 59 pg/mL) was lower than that of NPDR (538. 82 ± 187. 20 pg/mL;P=0. 002).
●CONCLUSION:The decrease of Dkk-3 level may be related to the occurrence and development of diabetic retinopathy, and there is a significant correlation with PDR. Circulating blood Dkk - 3 protein in diabetic retinopathy has a certain differential efficacy, it is likely to become diabetic retinopathy patients peripheral blood test indicators.

Animals ; Cadmium Compounds ; toxicity ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Cricetinae ; Cricetulus ; Fibroblasts ; drug effects ; metabolism ; Lactate Dehydrogenases ; metabolism ; Lung ; cytology ; Malondialdehyde ; metabolism ; Quantum Dots

BACKGROUND: Research on selective attention of mobile visual information is relatively scarce because of difficulties in technology, methodology and experimental condition control. As is well known, most visual information in the real world is mobile and changeable. To examine the mechanism of mobile visual information is helpful to designing natural and highly efficient human-computer interface as well as selecting specialized personnel in special fields OBJECTIVE: To explore the distractive effect of the number, color, and form changes of distractors on multiple-target tracking performance. DESIGN: The present study was a 3 × 2 × 2 within subject design. Participants were undergraduates from Beijing Normal University.SETTING: Institute of Cognitive Neuroscience and Learning, Beijing Normal University.PARTICIPANTS: The experiment was performed in the Experimental Psychology Lab of Beijing Normal University in April 2003. Twenty-four undergraduates from Beijing Normal University participated in the experiment. Among them there were 11 males and 13 females aged 17-25 years.They all reported normal or corrected-to-normal vision and normal color perception.METHODS: ① Stimuli were presented on a 17-inch Founder monitor with the resolution of 1028 × 800 pixels. A Founder computer controlled the stimulus presentation and randomization of trials. Response latencies were collected by keyboard response with accuracy of one millisecond. The stimuli were randomly moving "日" characters. The number of "日" characters is 4, 8 and 12. As the participants were tracking the objects, the targets would transform to "(│)" and "(│)", and the distractors would change to "(│)" , "(│)", or "H" by removing lines from "日 ". All distractors and targets were red at the beginning of moving. ② The correct responses were analyzed with 3-factor repeated measures analysis of variance, multiple comparison analysis, and pairwise T-test.MAIN OUTCOME MEASURES: Response time and accuracy of pressing the keys.RESULTS: The data of 24 participants were involved in the final statistical analysis. ① The three-factor repeated measures ANOVA results showed that the main effect of the number change of distractors was significant [F(1,22)=6.040, P ＜ 0.05]. The further ANOVA and pairwise comparison in different experiment conditions also showed no distractive effects when target and distractor had different shape but the same color [F(1,2,3)=9.965, P ＜ 0.01]. The further pairwise comparison indicated that when the number of distractors was 2 and 6, the participants' response latencies had no significant effects, and they had significant effect when the number of distractors was 2 and 10, or 6 and 10 (RT10-RT2=46 ms, P ＜ 0.05, two-tail test; RT10-RT6=28ms, P ＜ 0.05, one-tail test). These results showed that the increase in the number of distractors had distractive effects. ② There had no significant effect whether target and distractor had the same color or not, when the number of distractors was 2 and 10. And there had significant effects whether the color of target and distractor was the same or not when the number of distractors was 6; the same shape of target and distractor produced distractive effects (Tsame colons=1.926; Tdifferent colons=2.044,P ＜ 0.05, one-tail test). The main effect of the color was not significant. The further pairwise comparison showed the same result. That is,the color change of diatractors had no obvious distractive effect.CONCLUSION: ① There was distractive effect when the number of distractors increased while the shape and color of distractor and target were different. ② There was no distractive effect when the number of distractors increased while the shape of distractor and target was the same. ③ There was distractive effect when the number of distractors increased while the shape of distractor and target was different, and there was distractive effect when the color of distractor and target was the same.

BACKGROUND: Primary progressive aphasia(PPA) is a degenerative disease of nervous system, which is very rare in clinics. Only 3 cases have been reported in our country. There is very little clinical information regarding the characteristics of PPA in linguistics and imageology.OBJECTIVE: To report the language disorder and the characteristics of imageology of one PPA patient for the clarification of the clinical features of PPA.DESIGN: A case analysis.SETTING: Department of Neurology, Beijing Tiantan Hospital; Faculty of Psychology, Beijing Normal University.PATICIPANT: Male, 56 years old, senior high school graduate, businessman before the onset of the disease. The case visited the department of Neurology,Tiantan Hospital, Beijing due to the complaint of three years of progressive decreasing in language skills, which was then diagnosed with PPA.METH ODS: Spoken language fluency evaluation in Chinese Aphasia Examination Set of the First Affiliated Hospital of Beijing Medical University was used to evaluate this patient, the type of Aphasia was judged by Western Aphasia Examination Set, and the severity gradation was classified by the Boston Diagnostic Aphasia Examination(BDAE) . Memory, intelligence screening and imageology examination were performed as well.MAIN OUTCOME MEASURES: The fluency of spoken language, the type of aphasia, and the severity of aphasia of the patient were judged, and whether the patient suffered from memory and intelligence disorder were observed as well as the features of imageology.RESULTS: The patient had fluent spoken language, which was evaluated as sensory aphasia(SA) with the aphasia severity of level 4, and had no memory or intelligence disorder. MRI showed atrophy in left frontal lobe and temporal lobe, which was more significant in left side. MRI also showed that left temporal and frontal lobes had low metabolism and low perfusion.CONCLUSION: PPA is a disease with language disorder as its dominant clinical manifestation, which no other cognitive disorder at its initial stage,and with its main pathological changes in left temporal and frontal lobes.

Objectives: To assess the feasibility and stability of right ventricular outflow tract (ROVT) pacing under current technology by comparing the results of ROVT pacing with the traditional right ventricular apex (RVA) pacing. Methods: A total of 42 patients (at mean age of 63.5±10.4 years) without structural heart disease were randomly divided into two groups. RVA pacing group (n=14),and RVOT pacing group(n=28). An active fixation lead was implanted in all patients whose pacemaker could automatically measure the pacing threshold every day. The operation time,X-ray exposure time and lead parameters detected during the operation were collected to evaluate the feasibility of RVOT pacing. The complications related to lead and implantation procedure and the trend of threshold change during the follow-up time were used to assess the stability of RVOT pacing.Results: There were no statistic differences between RVA pacing group and RVOT pacing group in terms of operation time,X-ray exposure time and lead parameters. In RVOT group,the change of threshold during acute period was similar to those in RVA group (P=0.23). Chronic pacing threshold was also comparable between two groups,mean threshold at 6 months follow-up time was 0.55±0.11V and 0.54±0.09V at 0.4 pulse width in RVA group and RVOT group respectively (P=0.787).Conclusion: RVOT pacing was feasible and stable in operation time and lead characteristics compared with the conventional RVA pacing under current pacing technology.