Bile Duct Neoplasms ; pathology ; surgery ; Bile Ducts, Intrahepatic ; Cholangiocarcinoma ; pathology ; surgery ; Humans

Objective:To observe the effect on different mucosal sites and system immune sites after intranasal immunization with bivalent Shigella vaccines Methods:BALB/c mice were divided into three groups at random , 10 mice per group Mice were intranasally immunized respectively with FSM 2117or FS 5416 (4?10 7CFU) three doses with an interval of two weeks The NALT, NP, spleen, PP, MLN, lymphocytes were isolated on the seventh day after the last immunization to assay the change of the cell phenotype with FACS The nasal ?lung?intestine?genital tract lavage fluid and serum were taken to assay the specific IgA or IgG against F2a or Sonni LPS with ELISA Results:The specific IgA and IgG in different mucosal sites and serum increased significantly after intranasal immunization with two Shigella vaccines compared with the control (P

The community - based healthcare service is a main component of healthcare service system in China,and its development is affected by several factors including correlative ethical issues.In order to maintain a long - term and stable development of community - based healthcare service,great importance should be attached to some concerned ethical issues,such as the human - oriented service,intimacy of patients,the interests of disadvantaged group,etc.

Aged ; Antineoplastic Agents ; therapeutic use ; B7-2 Antigen ; analysis ; Diagnosis, Differential ; Follow-Up Studies ; Histiocytic Disorders, Malignant ; drug therapy ; metabolism ; pathology ; Humans ; Male ; Sarcoma ; chemistry ; drug therapy ; pathology ; Skin Neoplasms ; chemistry ; drug therapy ; pathology

Antigens, CD7 ; metabolism ; CD3 Complex ; metabolism ; Diagnosis, Differential ; Humans ; Ileal Neoplasms ; immunology ; pathology ; surgery ; Leukosialin ; metabolism ; Lymphoma, B-Cell, Marginal Zone ; immunology ; pathology ; Lymphoma, T-Cell ; immunology ; pathology ; surgery ; Male ; Middle Aged

OBJECTIVETo compare the contents of resveratrol and polydatin in some materials of Polygonum cuspidatum from various sources, so to screen and obtain the suitable cultures for the following metabolism regulation study.

METHODRP-HPLC method was applied to simultaneously assay resveratrol and polydatin in different samples.

RESULTBy the modified methods of extraction and determination, large amount of materials were screened. The results indicated that the contents of resveratrol and polydatin in root and rhizome were evidently higher than those in the leave and stems. The content of polydatin in the seedlings cultured indoor for three months was 1.27% and showed a 1.25-time increse than that in the wild plants, while the content of resveratrol (0.401%) approached that in the wild plants. Both of resveratrol and polydatin could be examined from different tissue cultures of P. cuspidatum, such as the sterile seedlings, callus, suspended cells and hairy roots, and the levels of them were closely related to the growth speed, physiological status and developmental phase. Hairy roots had the highest potentiality in several tested cultures and the increase rate of dry weight was 8.29 when cultured in vitro for 30 days, and showed a 8.4-fold and a 192.8-fold increase compared with those of natural roots and suspended cells, respectively. The content of polydatin in the hairy roots was up to 0.037% and that of resveratrol was 0.007%.

CONCLUSIONThe established analysis method is rapid, simple and accurate, especially adapted to the simultaneous determination of resveratrol and polydatin in massive biological samples. Hairy-root cultures have the superiority among the tested materials of P. cuspidatum and are suitable for the large-scale biomass and consistent production of efficient constituents.

Biomass ; Chromatography, High Pressure Liquid ; methods ; Fallopia japonica ; chemistry ; growth & development ; Glucosides ; analysis ; Plant Leaves ; chemistry ; growth & development ; Plant Roots ; chemistry ; growth & development ; Plant Stems ; chemistry ; growth & development ; Plants, Medicinal ; chemistry ; growth & development ; Reproducibility of Results ; Rhizome ; chemistry ; growth & development ; Seedlings ; chemistry ; growth & development ; Stilbenes ; analysis ; Tissue Culture Techniques ; methods

Adolescent ; Adult ; Aged ; Biopsy ; Delayed Graft Function ; diagnosis ; pathology ; Female ; Graft Rejection ; diagnosis ; pathology ; Humans ; Kidney ; pathology ; physiopathology ; Kidney Function Tests ; Kidney Transplantation ; pathology ; Male ; Middle Aged ; Young Adult

Antigens, CD20 ; analysis ; Biopsy, Needle ; Breast Neoplasms ; chemistry ; pathology ; Female ; Humans ; Lymphoma, B-Cell, Marginal Zone ; chemistry ; pathology ; Middle Aged ; Nipples ; pathology

OBJECTIVETo enhance the expression level of staphylococcal enterotoxin O (SEO) by optimization of rare codons.

METHODSThe gene of mature SEO (His-tag included) was cloned to pET28a, and 15 rare codons on the gene were optimized by PCR technology. These recombinant plasmids then were transformed into E.coli BL21(DE3), respectively. After IPTG induced, the expression levels of those mutants were analyzed by SDS-PAGE. The proteins were purified and their bioactivities were determined.

RESULTAfter the optimization of rare codons, the expression levels were increased from 7.49% to 19.8% in total cell proteins. The optimized SEO had bioactivity to stimulate the proliferation of murine lymphocytes, which was equivalent to that of non-optimized SEO in vitro.

CONCLUSIONOptimization of rare codons can enhance the expression of SEO effectively.

Animals ; Cloning, Molecular ; Codon ; genetics ; Enterotoxins ; biosynthesis ; genetics ; Escherichia coli ; genetics ; metabolism ; Mice ; Mutation ; Plasmids ; genetics ; Recombinant Proteins ; biosynthesis ; genetics ; Transformation, Bacterial