Oncomodulin(OM) is a kind of Ca-bind protein(CABP),and it belongs to the tropon in C super family.It different from other CABP because of its special domain(CD domain) which could bind with Ca2+.Multiple functions of OM have been determined during the study of its construction and distribution.It plays very important role in adjusting cell electric activity,regulating cell signal conduction passageway and cell cycle.In recent years,the study on OM promoting axon regeneration is emerging,especially in the regeneration of optical nerve.It offers a basis for solving the regeneration of spinal cord and cranial nerve.The concept,construction and distribution,action mechanism and its relationship with regeneration of optical nerve were reviewed in this article.

Objective To discuss the clinical significance of nephroscopic surgery for simple renal cysts. Methods A 2~4 cm incision was made below the 12th costal interspace. Through the incision the nephroscopic decortication was performed in 26 patients with simple renal cyst. Results The operation time was 20~80 min (mean, 30 min) and the postoperative hospital stay, 3~)6 days (mean, 4 days). No blood transfusion was required and no severe complications were noted. Follow-up observations for 3~)12 months (mean, 8 months) in the 26 patients found no recurrence. Conclusions Mini-incision nephroscopic decortication for renal cysts is feasible and mini-invasive, with advantages of simplicity of performance and quick postoperative recovery.

One hundred and twenty cases of osteosarcoma were reported.Re-examination of all the tissue specimens revealed that typical neoplastic osteoid was found only in 83% of the cases.It is now generally accepted that the demonstration of osteoid is not essential for the diagnosis,but the microscopic features of sarcomatous stroma,pheomorphism of osteoblasts,anaplastic giant cells,chondrosarcomatous and fihrosarcomatous tissue are of diagnostic importance.The differential diagnosis between neoplastid osteoid and pseudo-osteoid(fibrillar hyalin-ized collagen)were discussed.Forty-eight surgical specimens were stained with polyclonal actin,monoclonal BMP,vi-mentin,collagen type IV and UEA-1 with immunohistochemical ABC.It was found that when there was combined positive expression of monoclonal BMP,vimentin,collagen type IV,UEA-1 and polyclonal actin,or monoclonal BMP,vimentin UEA-1 and/or polyclonal actin,or monoclonal BMP,vimentin and/or polyclonal actin,it was of diagnostic value.However,the five markers were of no value to distinguish fibrillar hyalinized collagen from osteoid stroma.It is believed that the appropriate combination of the immunohistochemical markers is imperative to promote the accuracy of the pathological diagnosis of osteosarcoma and its differential diagnosis.

Objective To investigate the effect of propofol on hypoxic pulmonary vasoconstriction(HPV)and assess the underlying mechanism in rats.Methods Twenty male SD rats weighing 300-400 g were.anesthetizedwith intraperitoneal phenobarbital.Heart and lungs were removed after thoracotomy.Pulmonary.arterial rings 4 mmin length and 1.0-1.4 mm in diameter were prepared and suspended in Earl solution maintained at 37℃ with a pHof 7.40 and aerated with 20% O_2-5% CO_2-25% N_2.The rings were stimulated with phenylephrine(PE)10~(-6)mol?L~(-1) with different preloads(300,500,700,900,1 100 mg).The isometric tension of the arterial rings wasmeasured.The optimal preload was determined to be 900 mg which allowed best contractility.Hypoxia was inducedby aerating the solution with 95% N_2-5% CO_2 and the flow rate was adjusted to maintain PO_2 of the solution at40-20 mm Hg and pH at 7.40.HPV of the rings were recorded.Then propofol was added to achieve a finalconcentration of 1,3,10,30,100 ?mol?L~(-1) and HPV was again induced and the changes in HPV wererecorded.In addition the effects of propofol(10,30 ?mol?L~(-1))on vasoconstriction produced by KCI and PE werealso measured.Results The lower doses of propofol(10 ?mol?L~(-1))significantly inhibited HPV(P

Aldose reductase ( AR ) belonging to nicotinamide-adenine dinucleotide phosphate ( NADPH ) -dependent aldehyde-keto reductase superfamily, is the key rate-limiting enzyme in the polyol pathway which plays an important role in the body’s high-sugar metabolism. AR is widely present in the kidneys, blood vessels, lens, retina, heart, skeletal muscle and other tissues and organs, converts glucose to sorbitol which easy permeability of cell membranes, cause cell swelling, degeneration, necrosis, and have a close relationship with the development of chronic complications of diabetes mellitus. Diabetic retinopathy ( DR ) is a multifactorial disease, the exact cause is currently unknown, but polyol pathway has been demonstrated to play an important role in the pathogenesis of DR. Clinical risk factors such as blood sugar control, blood pressure and other treatments for DR only play a part effect of remission or invalid, if we can find out DR genes associated with the disease, this will contribute to a better understanding of the pathological mechanisms and contribute to the development of new treatments and drugs. The current research progress of AR, AR gene polymorphism, Aldose reductase inhibitors to DR was reviewed in this article.

Objective To investigate the efficacy and safety of radiofrequency catheter ablation for treating epicardial accessory pathway .Methods 8 patients with unsuccessful endocardial ablation of accessory pathway were mapped within coronary venous si-nus or middle cardiac vein and the radiofrequency catheter ablation was performed by the temperature control electrode .Results Ablation in 8 cases was successfully performed within coronary sinus or middle cardiac vein .There were no complications in all pa-tients .Conclusion Radiofrequency catheter ablation of epicardial accessory pathway within coronary sinus and middle cardiac vein is safe and effective .

Objective To investigate the interventional effect of multidimensional strategy to reduce the incidence of neonatal ventilator-associated pneumonia.Methods The patients who were admitted to the NICU department and received mechanical ventilation (MV) for more than 48 hours from October 2012 to September 2014 were recruited. The control group received the experienced interventions from October 2012 to September 2013, neonates from October 2013 to September 2014 were recruited as the intervention group receiving multidimensional controlling strategy, including bundle care, education, pro-cess and outcome surveillance and feedback on the practices. The compliance of before-after implementation of interventions were quantitatively evaluated,and the rate of VAP was compared between the two groups.Results The compliance rate of hand hygiene and the qualiifed rate of sputum suction, oral care, drain condensation from ventilator circuit, semi-recumbent posi-tion, and preventing of stress-ulcers were increased 17.0%, 11.4%, 14.7%, 18.2%, 37.5% and 56.3% respectively after implemen-tation of multidimensional strategy, and had statistical difference (χ2=36.47-294.36,P<0.01). But the qualiifed rate of antibiotic use only was 66.1% in the post-VAP bundle phases, and showed no statistical difference before and after(P>0.05). The VAP rate was 41.7 cases per 1000 MV-days during control group and 19.7 cases per 1000 MV-days during intervention group, had statis-tical signiifcance (P<0.05). But the rate of ventilator application showed no statistical difference between two group (P>0.05). ConclusionThe multidimensional strategy can effectively prevent the incidence of VAP.

Objective To investigate the remineralization effects of the Aominqing dental desensitizer and the fluoride dentifrice on the demineralized enamels. Methods Sixty-three teeth were randomly divided into three groups after demineralization , then was remineralized for eight days by using Aominqing dental desensitizer, fluoride dentifrice (1.1 g/L), and deionized water, respectively. The thin sections of teeth were analyzed under the con-focal laser scanning microscope (CLSM). The morphology of the surface of teeth was observed under the scanning electron microscope (SEM). Results Under CLSM, the evaluation parameter area of the fluorescent lesion (A,μm2) processed by Aominqing and by fluoride was (3.19 ± 0.19) × 104, (3.61 ± 0.26) × 104 μm2, respectively. The total fluorescence (TF) was (0.61 ± 0.09) × 106, (0.89 ± 0.15) × 106, average fluorescent of the lesion(AF) was (18.98 ± 1.56), (24.65 ± 2.39), and the above parameters were all less than those in the blank control group [A=(4.89 ± 0.24) × 104 μm2,TF=(1.78 ± 0.21) × 106, AF = 36.29 ± 2.57] (P < 0.01). The evaluation parameters in the Aominqing group were less than those in the fluoride dentifrice group(P < 0.05). Under SEM, the surface of the group processed by Aominqing was the smoothest, compared to the fluoride dentifrice group and the blank control group. Conclusions Both Aominqing dental desensitizer and fluoride dentifrice (1.1 g/L) have the remineralization effects on the demineralized enamels, and the former has a stronger effect.

Biomedical Research ; Humans ; Medicine, Chinese Traditional

Microtubule is one of the key components of the cytoskeleton and plays an important role in the maintenance of cell shape and the process of signal transduction and mitosis. Due to the extreme importance of microtubule in the process of mitosis, tubulin becomes one of the most important targets for development of new anticancer drugs and tubulin inhibitors are used for the treatment of cancer nowadays. These inhibitors have antitumor activity by inhibiting or promoting the assembly of tubulin to microtubules and interfering the process of cell mitosis. This review summarized the research progress of the tubulin inhibitors, especially the introduction of the tubulin inhibitors of pharmacological activities and the progress of clinical research. Also, the development trend of these inhibitors is discussed.