1.Advances in UL7 gene of herpesvirus.
Jie HUANG ; An-Chun CHENG ; Ming-Shu WANG
Chinese Journal of Virology 2011;27(5):501-504
Animals
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Herpesviridae
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genetics
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metabolism
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Humans
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Viral Proteins
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chemistry
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genetics
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metabolism
8.The Biosynthesis of ?-arbutin by Xanthomona maltophilia BT-112
Xiu-Peng WANG ; Shu-Rong ZHANG ; Chun-Qiao LIU ; Chun-Ying LIU ; Peng ZHANG ;
Microbiology 1992;0(03):-
?-arbutin is biosynthesized by whole cell method with Xanthomona maltophilia BT-112.The conditions for cell biosynthesized ?-arbutin are investigated as follows:temperature,25℃;concentration of hydroquinone,30mmol/L;mol ratio of sucrose and hydroquinone,20∶1;time course of ?-arbutin biosynthesis,45 hours;rotational speed,160r/min;concentration of Xanthomona maltophilia BT-112,85g/L;concentration of K-2HPO-4-KH-2PO-4 buffer solution,25mmol/L;pH of K-2HPO-4-KH-2PO-4 buffer solution,8.0.Under the above optimal conditions,the maximum of molar conversion yield based on the amount of hydroquinone supplied reaches 86.7%.
9.Proteomic analysis of the effects of tumor necrosis factor-? on endothelial cells
Jun-Ping LV ; Shu-Ren WANG ; Zeng-Chun MA ; Sheng-Qi WANG
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To investigate the affected proteins by tumor necrosis factor (TNF)-? in endothelial cells, and further explore the potential molecular mechanism of TNF-? on endothelial cells. METHODS: Nitric oxide (NO) production in the cultured human umbilical vein endothelial cells (HUVECs) was measured by a NO assay kit. Proteomic alterations were analyzed using two-dimensional electrophoresis, and peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. RESULTS: NO production in HUVECs decreased significantly after TNF-? treatement. Proteomics analysis showed 21 protein spots were changed including 9 spots that were increased and 11 spots that were decreased after TNF-? stimulation, and 1 spot was only detected in TNF-? activated cell gels. CONCLUSIONS: The decreased expression of ecNOS by TNF-? might result in decrease in NO production. Up-regulated MAP/ERK kinase 3 expression might imply that TNF-? activates the expression of adhesion molecules. Cytoskeletal protein actin is also involved in TNF-? injuried HUVECs. Proteomic analysis can find some clues for identifying new potential target of TNF-?. [
10.Study of EML4-ALK fusion gene as a biomarker in non-small cell lung cancer.
Lu WANG ; Yao-hui WANG ; Chun-yang LIU ; Mei HAN ; Shu-peng ZHANG ; Ren-sheng LAI
Chinese Journal of Pathology 2011;40(11):788-790
Biomarkers, Tumor
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metabolism
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Carcinoma, Non-Small-Cell Lung
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drug therapy
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metabolism
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pathology
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Humans
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Lung Neoplasms
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drug therapy
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metabolism
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pathology
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Oncogene Proteins, Fusion
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chemistry
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metabolism
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Protein Kinase Inhibitors
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therapeutic use
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Pyrazoles
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therapeutic use
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Pyridines
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therapeutic use
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Pyrimidines
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therapeutic use
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Smoking