Animals ; Depression ; etiology ; metabolism ; Frontal Lobe ; metabolism ; Glutamic Acid ; metabolism ; Male ; Nitric Oxide ; metabolism ; Rats ; Rats, Sprague-Dawley ; Serotonin ; metabolism ; Stress, Physiological ; physiology ; Swimming

Cardiovascular Diseases ; Follistatin-Related Proteins ; physiology ; Humans

Abortion, Induced ; adverse effects ; Acupuncture Therapy ; Adult ; Female ; Humans ; Infertility, Female ; etiology ; therapy ; Pregnancy

Chromatography ; methods ; DNA Fingerprinting ; Drugs, Chinese Herbal ; analysis ; Magnetic Resonance Spectroscopy ; Mass Spectrometry

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Arrhythmias, Cardiac ; diagnosis ; genetics ; therapy ; Child ; Child, Preschool ; Electrocardiography ; Humans ; Infant ; Male ; Middle Aged ; Syndrome

Objective To explore the normal reference values of infant intelligence in Fagan test of infant Method Fagan test was carried out in 315 healthy infants aged 6.5～14.0 month in Shenyang,Tieling and Jinzhou in Liaoning province.Results Infants with cognitive exponent X≥54.6 might be considered to be normal in development,49.9

Objective To evaluate the efficacy of 2-year treatment of interferon alpha combined with lamivudine therapy in chronic hepatitis B patients. Methods From 1999 to 2001 87 patients were enrolled and randomized into two groups.36 patients received interferon alpha and lamivudine simultaneously for 26 weeks,then lamivudine alone for 76 weeks.51 patients received lamivudine alone for 104 weeks.The efficacy was evaluated by biochemical and virological parameters.The YMDD motif was detected in both groups. Results There was no difference between two groups in HBV DNA response ( P =0.24).Proportion of HBeAg/Anti-HBe seroconversion in combination group was significantly higher than lamivudine group (38.9% vs 17.6%)( P =0.03).HBV YMDD mutation rate in combination group was lower than in lamivudine group (22.2% vs 43.1%, P =0.04). Conclusion The efficacy of combination therapy appeares to be better than lamivudine monotherapy.Combination treatment may delay or diminish the development of HBV YMDD mutation.

Objective To construct recombinant human Bcl-2 adenoviral vector. Methods Sense human Bcl-2 cDNA fragment from plasimid pcDNA3 1/bcl-2 was cloned into adenovirus shuttle vector pAdCMV. pAdCMV/bcl-2 and plasmid pJM17 were cotransfected into 293 cells with lipofectamine 2000. Replication defective adenovirus was reproduced and purified. Adenoviral virus DNA was detected with polymerase chain reaction(PCR), and plaque assay was performed to determine the titer of virus. Results Recombinant adenovirus containing bcl-2 target gene was obtained. The titer of virus was 2 0?10 10 pfu/ml. Conclusion High titer of adenovirus containing target gene was successfully obtained by recombinant adenovirus vector.

Animals ; Herpesviridae ; genetics ; metabolism ; Humans ; Viral Proteins ; chemistry ; genetics ; metabolism

Codon ; Herpes Simplex ; virology ; Herpesvirus 1, Human ; genetics ; metabolism ; Humans ; Protein Binding ; Protein Biosynthesis ; Viral Proteins ; genetics ; metabolism