Objective To study the effect of chest wall vibration therapy on bronchiolitis. Methods A total of 64 patients with bronchiolitis were divided into an experimental group and a control group, the former included 34 cases and the latter included 30 cases. The experimental group received both routine treatment and chest wall vi- bration, while the control group only received routine treatment. PaO_2, PaCO_2, SaO_2, Heart Rate (HR) and Respi- ration (R) were observed, respectively, in the experimental group and the control group at the beginning and the end of the third day. Time needed for expectoration and length of hospital stay in the two groups were observed. Results It was shown that PaO_2, PaCO_2, SaO_2 , HR, R were significantly improved at the end of the third day when compared with those at the beginning in both groups(P

Objective To evaluate the effectiveness of biofeedback in preventing chronic daily headaches. Methods One hundred patients experiencing daily headaches were randomly divided into a biofeedback group ( n=50) and a drug therapy group (n=50). The patients in the drug therapy groupwere administered a predetermined course of medication. Those in the biofeedback group were given 30 minutes of biofeedback therapy twice a week for 8 weeks, followed by 10 months of intensive therapy once a month. The headache frequency, duration of headache at-tacks, days of using acute pain medication and any other adverse events were recorded 3, 6 and 12 months after the treatment. Results The patients in the biofeedback group had significantly less-frequent headaches, shorter headache attacks and fewer days of using acute pain medications. Conclusion Compared to drug therapy, biofeed-back can prevent chronic daily headachesmore safely and effectively.

OBJECTIVETo investigate the apoptosis and proliferation activity of Tca8113 cells.

METHODSThe vector that involves short hairpin RNA of iNOS was transfected to Tca8113 cells. The change of iNOS expression was observed using immunohistochemistry technique, the apoptosis rate examined by flow cytometry, and the proliferation Tca8113 cells examined by methyl thiazolyl tetrazolium (MTT).

RESULTSThe expression of iNOS in Psilencer-iNOS group was lower than that in control groups (P < 0.01), the apoptosis rate was higher than that in control groups (P < 0.01); whereas the proliferation activity of Tca8113 cells in Psilencer-iNOS group was lower than that in control groups.

CONCLUSIONSDown expression of iNOS by RNAi can promotes apoptosis of Tca8113 cells and has an anti-proliferation activity effect.

Apoptosis ; Carcinoma, Squamous Cell ; genetics ; pathology ; Cell Line, Tumor ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Genetic Vectors ; Humans ; Nitric Oxide Synthase Type II ; genetics ; metabolism ; Plasmids ; genetics ; RNA Interference ; Tongue Neoplasms ; genetics ; pathology ; Transfection

In the context of internationalization of education,the military graduate education concepts and models also come to change,and opportunities and challenges coexist.In this article,the challenges and problems of army medical college graduate education were mentioned and analyzed,and the exploration and attempt of graduate education in the process of international were summarized.

OBJECTIVETo study the anti-glioma effect of recombinant adenovirus mediated combined gene therapy of bFGF-siRNA and HIV1-Vpr in vivo.

METHODSMouse glioma model was established by injecting 5 × 10(6) LN229 cells into BALB/c-nu nude mice. 30 nude mice were randomly divided into 5 groups: the negative control group, mock group, bFGF-siRNA group, Vpr group and combined therapy group, which at regular intervals were injected with PBS, rAd5-null, rAd5-bFGF-siRNA, rAd5-Vpr, rAd5-bFGF-siRNA plus rAd5-Vpr, respectively. The tumor volume was recorded every third day to draw a growth curve. After four weeks treatment, the mice were killed and specimens were taken. HE, immunohistochemical and TUNEL staining were performed to observe the cell morphology, detect the changes of relevant target proteins and cell apoptosis, respectively. Also the ultrastructural changes were observed by electron microscopy.

RESULTSThe tumor growth inhibition rates were 36.9%, 37.2% and 58.6% in the bFGF-siRNA group, Vpr group and combined therapy group, respectively, and the combined therapy group showed the most significant effect (P < 0.05). Also the results of HE, immunohistochemical and TUNEL staining revealed that the combined therapy group had the best effects on proliferation inhibition and apoptosis induced in glioma cells (P < 0.05). The most significant ultrastructural changes were observed in the combined therapy group.

CONCLUSIONThe combined gene therapy of bFGF-siRNA with Vpr shows a prominent and synergistic anti-glioma effect compared with that of mono-gene therapy in nude mice.

Adenoviridae ; genetics ; Animals ; Apoptosis ; Brain Neoplasms ; metabolism ; pathology ; therapy ; Cell Line, Tumor ; Cell Proliferation ; Fibroblast Growth Factor 2 ; genetics ; metabolism ; Gene Products, vpr ; genetics ; metabolism ; Genetic Therapy ; Glioma ; metabolism ; pathology ; therapy ; HIV-1 ; genetics ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; RNA, Small Interfering ; genetics ; Random Allocation ; Recombinant Proteins ; genetics ; metabolism

Objective To study the effect of atorvastatin on the expressions of intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-β1 (TGF-β1) in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE).Methods Eighty healthy female Wistar rats were randomly divided into 4 groups:normal control group (n=20),EAE model group (n=20),low-dose treatment group (n=20) and high-dose treatment group (n=20).Each group was equally divided into 2 sub-groups:14-d group and 21-d group according to the day that they were sacrificed.EAE rat models were established by immunizing the rats with fresh guinea-pig spinal cord homogenate plus complete Freund's Adjuvat (CFA).Rats of the treatment groups were fed with atorvastatin at dasage of 2 mg/(kg·d)and 10 mg/ (kg·d),respectively; and rats of the normal control group and EAE model group were fed with physiological saline.The severity of EAE was scored according to the signs and symptoms,and NIHSS was performed.Pathological changes were observed with the aid of HE staining.The levels of ICAM-1 and TGF-β1 were detected by immunohistochemistry.Results The rats in the high-dose treatment group had significantly lower incidence of disease,decreased CNS inflammation focus,lower level of ICAM-1 and higher level of TGF-β1 as compared with rats of the EAE model group and low-dose treatment group (P＜0.05).Conclusion Atorvastatin could ameliorate EAE of rats in a dose-dependent manner,whose effect might be related to the decreased expression of ICAM-1 and increased expression of TGF-β1.

Objective To investigate the relationship of the serum chemokine RANTES level in patients with chronic hepatitis B among different clinical severity and to explore its passible reasons. Methods 144 patients with chronic hepatitis B were divided into mild-moderate(46), serious(51) or severe group(47) according to the different clinical severity and 18 normal cases were taken as normal control. The serum level of chemokine RANTES was detected with an ABC-ELISA assay. Statistical analysis was performed on the software of SPSS 13.0.Results The serum chemokine RANTES levels in patients with chronic hepatitis B (2227.06±790.80, 5878.49±3334.58, 3482.77±2315.62 ng/L in mild-moderate, serious and severe group respectively) were significantly higher than that in the normal control (329.46±152.00 ng/L). The differences between each two hepatitis groups were also statistically significant (P<0.05) and serum chemokine RANTES level in serious group was highest among them. Conclusion Serum chemokine RANTES level in patients with chronic hepatitis B elevates significantly and it might play an important role in the pathogenesis of chronic hepatitis B.

Objective The aim of this study was to investigate the relationship between liver function test,serum HBeAg,HBV DNA level and liver pathological changes in patients with chronic hepatitis B.Methods 233 patients with chronic hepatitis B accept liver functure biopsy,liver function test,HBeAg detection and HBV DNA fluorescent quantitation PCR detection. Comparisons of liver function test,HBeAg and HBV DNA level were conducted among different liver pathological changes including inflammation grading and fibrosis staging. Results In different inflammation grading groups,ALT was highest in group G3 and lowest in group G0-1 ( P = 0.016) ;TBil was highest in group G4 and lowest in group G0-1 (P = 0.000);HBV DNA level was highest in group G4 and lowest in group G0-1 ,but not statistically significant among groups ( P = 0.463). in different fibrosis staging groups,ALT was highest in group S3 and lowest in group S0-1 ,but not statistically significant among groups (P = 0.562) ;TBil was highest in group S4 and lowest in group S2 ( P = 0.039) ; HBV DNA level was highest in group S3 and lowest in group S0-1 ,but not statistically significant among groups ( P = 0.395). In HBeAg positive group,the proportion of G3-4 in inflammation grading or S3-4 in fibrosis staging was lower than that in HBeAg negative group (46% vs 52%,P = 0.438;38 % vs 53 %,P = 0.025 ;respectively). Conclusion HBV DNA level can not indicate the severity of liver inflammation or fibrosis in chronic HBV infection. Patients with HBeAg negative often are complicated with more severity of liver fibrosis. In routine liver function test,TBil level eorrelates with hver inflammation grading or fibrosis staging; ALT level also correlates with liver inflammation grading but not with fibrosis staging.

Objective To study the pathological features of liver tissues from patients clinically diagnosed with mild chronic hepatitis B based on current guideline and emphasize the important significance of liver puncture and biopsy for these patients. Methods Totally 156 patients clinically diagnosed with mild chronic hepatitis B based on current guideline received liver puncture under the real-time Doppler ultrasonographic guiding. Pathological diagnosis was made after microscopic examinations of the liver tissue specimens stained with hematoxylin-eosin (HE) and reticular fiber staining. The differences between clinical and pathological diagnosis for these patients were analyzed. Results Finally, 105 (67.3%) patients were pathologically diagnosed with mild chroniehepatitis B; 28 (18.0%), 3 (1.9%) and 20 (12.8%) patients were pathologically diagnosed as moderate, severe chronic hepatitis B and cirrhosis, respectively. Forty-eight (30.8%) and 39 (25.0%) patients of non-mild chronic hepatitis B were found to have G3-4 inflammation and S3-4 fibrosis, respectively. Differences in serum alanine aminotransferase, aspartate aminotransferase, total bilirubin or albumin between mild and non-mild chronic hepatitis B based on pathological diagnosis were not statistically significant (t-test, P > 0.05 ). Comclusions Accurate pathological diagnosis is helpful to guiding an antiviral therapy.

Objective To investigate the level of the serum chemokine RANTES and its correlation with serum biochemical indices of liver function test, HBeAg and HBV DNA load in patients with chronic hepatitis B.Methods 144 patients with chronic hepatitis B (observed group) and 18 normal cases (control group) were enrolled in this study. The serum level of chemokine RANTES was detected with an ABC-ELISA assay. Statistical analysis was performed on the software of SPSS13.0. Results The serum chemokine RANTES level in the observed group (3930.12 ng/ml±2856.96) ng/ml was significantly higher than that in the control group (329.46 ng/ml±152.23) ng/ml. The results from the observed group indicated the positive correlation of serum RANTES level with indices of liver function test, including ALT (r=0. 197, P=0.018), AST(r=0.239, P=0.004) and TBil (r=0.316, P=0.001), but did not with PTA (r=-0.078, P=0.357). Neither difference of serum chemokine RANTES level between HBeAg-positive group and HBeAg-negative group nor that between high HBV DNA load group (≥105 copies/ml) and low HBV DNA load group (< 105 copies/ml) were statistically significant (P=0.407 and 0.185, respectively). Conclusions Serum chemokine RANTES level in patients with chronic hepatitis B elevates significantly and is not affected by HBeAg or HBV DNA load. Its positive correlation with indices of liver function test indicates that RANTES might play an important rule in the pathogenesis of chronic hepatitis B.