Objective To study the effect of chest wall vibration therapy on bronchiolitis. Methods A total of 64 patients with bronchiolitis were divided into an experimental group and a control group, the former included 34 cases and the latter included 30 cases. The experimental group received both routine treatment and chest wall vi- bration, while the control group only received routine treatment. PaO_2, PaCO_2, SaO_2, Heart Rate (HR) and Respi- ration (R) were observed, respectively, in the experimental group and the control group at the beginning and the end of the third day. Time needed for expectoration and length of hospital stay in the two groups were observed. Results It was shown that PaO_2, PaCO_2, SaO_2 , HR, R were significantly improved at the end of the third day when compared with those at the beginning in both groups(P

Objective To evaluate the effectiveness of biofeedback in preventing chronic daily headaches. Methods One hundred patients experiencing daily headaches were randomly divided into a biofeedback group ( n=50) and a drug therapy group (n=50). The patients in the drug therapy groupwere administered a predetermined course of medication. Those in the biofeedback group were given 30 minutes of biofeedback therapy twice a week for 8 weeks, followed by 10 months of intensive therapy once a month. The headache frequency, duration of headache at-tacks, days of using acute pain medication and any other adverse events were recorded 3, 6 and 12 months after the treatment. Results The patients in the biofeedback group had significantly less-frequent headaches, shorter headache attacks and fewer days of using acute pain medications. Conclusion Compared to drug therapy, biofeed-back can prevent chronic daily headachesmore safely and effectively.

OBJECTIVETo investigate the apoptosis and proliferation activity of Tca8113 cells.

METHODSThe vector that involves short hairpin RNA of iNOS was transfected to Tca8113 cells. The change of iNOS expression was observed using immunohistochemistry technique, the apoptosis rate examined by flow cytometry, and the proliferation Tca8113 cells examined by methyl thiazolyl tetrazolium (MTT).

RESULTSThe expression of iNOS in Psilencer-iNOS group was lower than that in control groups (P < 0.01), the apoptosis rate was higher than that in control groups (P < 0.01); whereas the proliferation activity of Tca8113 cells in Psilencer-iNOS group was lower than that in control groups.

CONCLUSIONSDown expression of iNOS by RNAi can promotes apoptosis of Tca8113 cells and has an anti-proliferation activity effect.

Apoptosis ; Carcinoma, Squamous Cell ; genetics ; pathology ; Cell Line, Tumor ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Genetic Vectors ; Humans ; Nitric Oxide Synthase Type II ; genetics ; metabolism ; Plasmids ; genetics ; RNA Interference ; Tongue Neoplasms ; genetics ; pathology ; Transfection

OBJECTIVETo evaluate the relationship of the expression of bone morphogenefic protein-7 (BMP-7) in the liver and the liver fibrosis and inflammation in patients with chronic hepatitis B virus (HBV) infection, to explore the role of BMP-7 in the fibrogenesis of chronic hepatitis B.

METHODS81 patients chronically infected with HBV were enrolled. Liver biopsy were performed in all these patients. The hematoxylin staining and reticular fiber staining were performed for the grading of inflammation and staging of fibrosis, respectively. The patients were divided into different groups by the inflammatory grade or fibrosis stage (8, 14, 19, 22, 18 patients with inflammatory grade of G0, G1, G2, G3, G4, respectively; 8, 16, 21, 24, 12 patients with fibrosis stage of S0, S1, S2, S3, S4, respectively ). Then the immunohistochemical staining by BMP-7 were performed. The expression of BMP-7 in the liver was evaluated by digital image quantitative analysis system. We compared the expression of BMP-7 in the liver with different inflammatory grade and fibrosis stage.

RESULTSThe expression of BMP-7 in the liver tissue increased with the increase of hepatic inflammatory grade and fibrosis. When liver biopsy showed a severe intrahepatic inflammation, the expression of BMP-7 significantly increased, regardless the intrahepatic fibrosis stage. Similarly, when liver biopsy showed a severe intrahepatic fibrosis, the expression of BMP-7 also significantly increased, regardless the intrahepatic inflammatory grade.

CONCLUSIONBMP-7 may play an important role as anti-inflammatory and anti-fibrogenic effect in the fibrogenesis of chronic hepatitis B.

Bone Morphogenetic Protein 7 ; genetics ; metabolism ; Female ; Hepatitis B, Chronic ; genetics ; metabolism ; Humans ; Immunohistochemistry ; Liver ; immunology ; metabolism ; pathology ; Liver Cirrhosis ; genetics ; metabolism ; Male ; Reverse Transcriptase Polymerase Chain Reaction

OBJECTIVETo observe the efficacy and safety on the efficacy of HBeAg-positive chronic Hepatitis B patients treated with adefovir dipivoxil for 4 years.

METHODSNinety-five patients with HBeAg-positive chronic hepatitis B were treated with adefovir dipivoxil 10 mg per day orally. The patients were observed before and after treatment for their serum levels of ALT and HBV DNA, the new increasing rates of serum ALT normalization, HBV DNA clearances, HBeAg loss, HBeAg seroconversion and adverse drug events.

RESULTSAt 4 years on study, the rates of ALT normalization, HBV DNA clearances, HBeAg loss, HBeAg seroconversion and HBV DNA rebound were 89.5%, 63.2%, 47.4%, 41.1% and 8.0%, respectively. No drug related to renal function impairment was found during the treatment, eight patients had adverse drug events but all were mild.

CONCLUSIONAdefovir dipivoxil could effectively inhibit HBV replication, normalize ALT and enhance transformation from HBeAg to HBeAb for cases with naive and treated-first patients. The efficacy were increased with prolongation of the treatment period. It is safe and has a good tolerance.

Adenine ; administration & dosage ; adverse effects ; analogs & derivatives ; Adult ; Antiviral Agents ; administration & dosage ; Female ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; drug effects ; physiology ; Hepatitis B, Chronic ; blood ; drug therapy ; virology ; Humans ; Male ; Middle Aged ; Organophosphonates ; administration & dosage ; adverse effects ; Virus Replication ; drug effects

OBJECTIVETo investigate the relationship of the serum chemokine RANTES level in patients with chronic hepatitis B among different clinical severity and to explore its possible reasons.

METHODS144 patients with chronic hepatitis B were divided into mild-moderate (46), serious (51) or severe group (47) according to the different clinical severity and 18 normal cases were taken as normal control. The serum level of chemokine RANTES was detected with an ABC-ELISA assay. Statistical analysis was performed on the software of SPSS 13.0.

RESULTSThe serum chemokine RANTES levels in patients with chronic hepatitis B (2227.06 +/- 790.80, 5878.49 +/- 3334.58, 3482.77 +/- 2315.62 ng/L in mild-moderate, serious and severe group respectively) were significantly higher than that in the normal control (329.46 +/- 152.00 ng/L). The differences between each two hepatitis groups were also statistically significant (P < 0.05) and serum chemokine RANTES level in serious group was highest among them.

CONCLUSIONSerum chemokine RANTES level in patients with chronic hepatitis B elevates significantly and it might play an important role in the pathogenesis of chronic hepatitis B.

Adolescent ; Adult ; Aged ; Case-Control Studies ; Chemokine CCL5 ; blood ; Female ; Hepatitis B, Chronic ; blood ; immunology ; Humans ; Male ; Middle Aged ; Young Adult

OBJECTIVETo study the anti-glioma effect of recombinant adenovirus mediated combined gene therapy of bFGF-siRNA and HIV1-Vpr in vivo.

METHODSMouse glioma model was established by injecting 5 × 10(6) LN229 cells into BALB/c-nu nude mice. 30 nude mice were randomly divided into 5 groups: the negative control group, mock group, bFGF-siRNA group, Vpr group and combined therapy group, which at regular intervals were injected with PBS, rAd5-null, rAd5-bFGF-siRNA, rAd5-Vpr, rAd5-bFGF-siRNA plus rAd5-Vpr, respectively. The tumor volume was recorded every third day to draw a growth curve. After four weeks treatment, the mice were killed and specimens were taken. HE, immunohistochemical and TUNEL staining were performed to observe the cell morphology, detect the changes of relevant target proteins and cell apoptosis, respectively. Also the ultrastructural changes were observed by electron microscopy.

RESULTSThe tumor growth inhibition rates were 36.9%, 37.2% and 58.6% in the bFGF-siRNA group, Vpr group and combined therapy group, respectively, and the combined therapy group showed the most significant effect (P < 0.05). Also the results of HE, immunohistochemical and TUNEL staining revealed that the combined therapy group had the best effects on proliferation inhibition and apoptosis induced in glioma cells (P < 0.05). The most significant ultrastructural changes were observed in the combined therapy group.

CONCLUSIONThe combined gene therapy of bFGF-siRNA with Vpr shows a prominent and synergistic anti-glioma effect compared with that of mono-gene therapy in nude mice.

Adenoviridae ; genetics ; Animals ; Apoptosis ; Brain Neoplasms ; metabolism ; pathology ; therapy ; Cell Line, Tumor ; Cell Proliferation ; Fibroblast Growth Factor 2 ; genetics ; metabolism ; Gene Products, vpr ; genetics ; metabolism ; Genetic Therapy ; Glioma ; metabolism ; pathology ; therapy ; HIV-1 ; genetics ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; RNA, Small Interfering ; genetics ; Random Allocation ; Recombinant Proteins ; genetics ; metabolism

OBJECTIVETo analyze different clinical features from patients with chronic hepatitis B between HBeAg negative and positive.

METHODS354 patients with chronic hepatitis B (124 cases with HBeAg positive and 230 cases with HBeAg negative) were enrolled into this retrospective investigation. Comparisons were conducted according to their demographic, liver biochemical, virological characters and clinical diagnosis types.

RESULTS(1) patients with chronic hepatitis B in HBeAg negative group have older age; moderate and severe chronic hepatitis occupied a lower proportion (P = 0.007 and 0.014). But fulminant hepatitis had a higher proportion (P = 0.008). (2) ALT, ALB, PTA and HBV DNA load of HBeAg negative group were lower than that of HBeAg positive group, but TBil in HBeAg negative group was higher; AST had no statistical significance between two groups. (3) In high HBV DNA load group ( > 10(5) copies/ml), HBeAg negative group had a lower proportion than HBeAg positive group (37.4% vs 55.6%, P = 0.001).

CONCLUSIONHBeAg-negative patients compared with HBeAg-positive patients had older age, lower serum HBV DNA level and other characteristics; HBeAg-negative patients maybe had serious disease.

Adult ; Age Factors ; DNA, Viral ; blood ; Female ; Hepatitis B e Antigens ; blood ; Hepatitis B, Chronic ; blood ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Young Adult

OBJECTIVETo investigate the level of the serum chemokine RANTES and its correlation with serum biochemical indices of liver function test, HBeAg and HBV DNA load in patients with chronic hepatitis B.

METHODS144 patients with chronic hepatitis B (observed group) and 18 normal cases (control group) were enrolled in this study. The serum level of chemokine RANTES was detected with an ABC-ELISA assay. Statistical analysis was performed on the software of SPSS13.0.

RESULTSThe serum chemokine RANTES level in the observed group (3930.12 ng/ml 2856.96) ng/ml was significantly higher than that in the control group (329.46 ng/ml +/- 152.23) ng/ml. The results from the observed group indicated the positive correlation of serum RANTES level with indices of liver function test, including ALT (r = 0.197, P = 0.018), AST(r = 0.239, P = 0.004) and TBil (r = 0.316, P = 0.001), but did not with PTA (r = - 0.078, P = 0.357). Neither difference of serum chemokine RANTES level between HBeAg-positive group and HBeAg-negative group nor that between high HBV DNA load group (> or = 10(5) copies/ml) and low HBV DNA load group (< 10(5) copies/ml) were statistically significant (P = 0.407 and 0.185, respectively).

CONCLUSIONSSerum chemokine RANTES level in patients with chronic hepatitis B elevates significantly and is not affected by HBeAg or HBV DNA load. Its positive correlation with indices of liver function test indicates that RANTES might play an important role in the pathogenesis of chronic hepatitis B.

Adolescent ; Adult ; Aged ; Case-Control Studies ; Chemokine CCL5 ; blood ; Female ; Hepatitis B Surface Antigens ; blood ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; isolation & purification ; physiology ; Hepatitis B, Chronic ; blood ; diagnosis ; virology ; Humans ; Liver Function Tests ; Male ; Middle Aged ; Viral Load ; Young Adult

In the context of internationalization of education,the military graduate education concepts and models also come to change,and opportunities and challenges coexist.In this article,the challenges and problems of army medical college graduate education were mentioned and analyzed,and the exploration and attempt of graduate education in the process of international were summarized.