Objective:To explore the diagnostic value of multi-tumor marker protein biochip detective system for primary hepatic cancer.Methods:Using pathological examination,or at least one-year clinical follow-up results for the gold standard,we divided patients with liver disease into three two groups:primary liver cancer group,benign liver disease group and normal group,using protein chip technology to detect the measurement serum tumor markers.Results:CA199,CEA,CA242,FER,AFP,CAl25 and CA153 were significantly higher in PHC group than those in benign liver disease group and normal group(P

Objective To evaluate the safety of epidural resiting and the incidence of postdural puncture headache following accidental dural puncture. HZ Methods Among 63 patients of ASA I-II undergoing lower abdominal or lower extremity surgery, forty-two with postdural puncture who had received epidural resiting were randomly assigned to patient controlled epidural analgesia (PCEA) ( n =21) or control treatment ( n =21). The remaining 21 patients who had successful epidural insertion received the routine PCEA. All patients after operation received 48-hours monitoring of BP, HR, RR and SpO_2. The analgesic effect of each treatment and the occurrence of headache were also assessed. Results All operations were uneventful under epidural anesthesia. A satisfactory level of analgesia was observed in patients who received PCEA. There was no statistically significant difference in pain control between patients who had had epidural resiting and who received PCEA and those who received routine PCEA. No respiratory inhibition was observed. The occurrence of headache in the epidural resiting group was 19% (4/21), compared to 57% (12/21) in the control group ( P

Objective To investigate the therapeutic effect of distal radius fractures operation.Methods Eighty distal radius fractures patients who were treated from January 2010 to January 2011 were divided into observation group and control group by random digits table method,each group had 40 cases.The observation group was treated with plate internal fixation,the control group took closed reduction and splint external fixation.The palmar tilt,radial ulnar deviation,axial shortening before treatment and after treatment for 3 months were compared between two groups.The clinical curative effect after treatment for 1 year was compared.Results The palmar tilt,radial ulnar deviation,axial shortening before treatment between two groups had no significant difference (P ＞ 0.05).After treatment for 3 months,the palmar tilt,radial ulnar deviation,axial shortening in observation group were (17.0 ± 1.6)°,(23.0 ± 2.1)°,(1.0 ± 0.2) mm,in control group were (4.0 ± 0.8)°,(10.0 ± 1.1)°,(4.0 ± 1.1) mm,there was significant difference(P＜ 0.05).After treatment for 1 year,the excellent and good rate in observation group was 90.0％(36/40),significantly higher than that in control group [50.0％ (20/40)] (P ＜ 0.05).Conclusion Plate internal fixation in the treatment of distal radius fractures is more conductive to the wrist recovery of joint structure and function,reduce the occurrence of wrist joint dysfunction,improve the quality of life,and is worth to be applied in clinic.

Objective:To screen auramine O in Guci tablets. Methods:Auramine O was detected by HPLC method on a Diamon-sil-C18(250mm×4.6mm,5μm)columnwiththemobilephaseofacetonitrile-0.025mol·L-1monopotassiumphosphatebuffersolu-tion(containing 0. 2% triethylamine,adjusting pH to 3. 0 with phosphoric acid)(36∶65)at the flow rate of 0. 8 ml·min-1. The detec-tion wavelength was 432 nm and the column temperature was 35℃. Results:The LOD of auramine O was 0. 05ng. Among 45 batches of tested samples, auramine O was found in seven ones. Conclusion:The method is simple, rapid and accurate, which can guarantee the drug safety of Guci tablets.

Equipment Design ; Humans ; Pacemaker, Artificial

Objective: To establish a method for the determination of the related substances in pantoprazole sodium capsules. Methods:A Kromasil C18 column (250 mm × 4. 6 mm, 5 μm) was used. 0. 01 mol·L-1 monopotassium phosphate solution (adjus-ting pH to 7. 0 with phosphoric acid)-acetonitrile was adopted as the mobile phase with gradient elution. The detection wavelength was 289 nm and the column temperature was 40℃. The injection volume was 20μl and the flow rate was 1. 0 ml·min-1 . Results:Panto-prazole sodium and its degradation substances could be well separated. The limit of detection and quantification of pantoprazole sodium was 0. 16 ng and 0. 48 ng, respectively. Conclusion:The method is simple, specific and sensitive, and can be applied to determine the related substances in pantoprazole sodium.

Objective To investigate the retrieval mechanism of prospective memory,spatiotemporal patterns of event-related potentials (ERP) were used to reveal neural correlates of the prospective interference effect. Methods A task of matching two sequential patterns was taken as the ongoing task and the detection of patterns with two salient levels as prospective memory tasks. Experiments were performed in blocks for single ongoing task block and two prospective memory blocks. Results No significant behavioral effect was found for prospective interference [reaction time(ms):control blocks 750.29±161.33,high salience blocks 770.94±174.89,low salience bocks 764.79±174.12,F(1.52,25.82)=0.48,P =0.57 and correction rate(%):control blocks 85.89±8.07,high salience blocks 88.26±7.00,low salience blocks 87.91±7.28,F(1.50,25.57)=2.46,P =0.12].But significant ERP effect was found at bilateral frontal regions from 160 ms to 240 ms for both two prospective memory blocks. Moreover,greater negativity at the right frontal polar region appeared significantly at 360'440 ms for higher salient patterns. Conclusion Prospective memory is retrieved based on strategic monitoring and high salient stimuli may enhance to keep the prospective goal and improve task switching.

Objective To investigate the clinic significance of serum carbohydrate antigen ( CA)125 for cirrhosis. Methods 88 patients with type B posthepatitic cirrhosis (male 46,female 24,average age 45?24 years) were divided into three group (A stage35,B stage 28,C stage 25) according to Child-pugh integration. 36 healthy persons as normal controls were studied(male 20,female 16,average age 42?22 years). Serum CA125 was measured by ELISA. We investigated the alteration of serum CA125 with hepatic functional classification. Results The levels of serum CA125 in cirrhosis patients were markedly higher than those in control subjects(P

A better understanding of the function of spleen has been gained recently,owing to the in-depth studies on the structure,cellular function,secretion and innervation of spleen.It is generally accepted that the spleen is the important part of the regulation network between immune,nerve and endocrine system.The spleen has much more functions besides blood filtering and storing,haematogenesis,and immunization,and its immune function has characteristics of two-way and phase.But further study is still needed for the precise evaluation of function of the spleen,especially the spleen on the pathologic state,clarification of the pathogenesis of hypersplenism,and the relationship between spleen and other organs.Here we introduced the progress of fundamental research of the spleen from multiple perspectives.

OBJECTIVE:To investigate Smad3 and Smad7 expression in cardiac tissues in myocardial infarction (MI) rats and the effect of simvastatin (SIM) on expression of Smads.METHODS:36 rats were randomized into SIM (40 mg?kg-1,n=12) group,MI group (normal saline,n=12) and SHAM group (normal saline,n=12).Rats in first two groups were induced MI model.Rats of 3 groups were sacrificed after four weeks administration.The mRNA expressions of Smad3 and Smad7 in non-infarction regions were measured by RT-PCR.Smad3 and Smad7 protein expressions were measured by Western blotting assay.RESULTS:As compared with SHAM group,the expressions of Smad3 in MI group and SIM group were increased while the expressions of Smad7 were decreased.As compared with MI group,the mRNA expression and protein expression of Smad3 in SIM group was decreased while that of Smad7 increased (P