Total hip arthroplasty is common joint replacement method in senile patients,which is characterized by large wound,large amount of bleeding,and great stimulation.Anesthesia methods during total hip arthroplasty include epidural anesthesia,epidural and lumbar anesthesia,and general anesthesia.The anesthesia method is dependent on patient condition.Appropriate anesthesia based on disease condition can reduce anesthesia risk.The key of anesthesia is to control anesthesia plane,supplement blood and monitor toxic reaction during cement application.Because senile patients are always complicated by respiratory,circulatory and endocrine system diseases,comprehensive treatment before surgery to improve body symptoms can increase anesthesia and surgery tolerance and better guarantee patient safety during surgery.

Objective To establish and optimize the method of isolation and cultivation of rat bone marrow mesenchymal stem cells(MSCs),and to investigate the condition and capability of hepatic differentiation of MSCs.(Methods) Rat bone marrow cells were collected from rats of 5～6 week and mononeuclear cells were isolated by Percoll density gradient centrifugation.MSCs were cultivated and purified by adherence method.Morphology,RT-PCR and immunocytochemistry were used to identify the role of substrates,the kinds of the cytokines and concentrations of cytokines on the hepatic differentiation potential.Results The quality and quantity of the isolated bone marrow mononuclear cells reached the peak by using 57% Percoll density gradient centrifugation.Discarding the suspending cells after 24h incubation and subculturing the cells with low seeding density were helpful for acquiring MSCs with improved reproductive capability and active function.MSCs could be successfully induced under HGF/FGF-4 induction on the substrate of(10 ?g/mL) fibronectin.MSCs exhibited round in shape after differentiation,instead of fibroblast-like morphology before.Albumin mRNA and protein were positively expressed in MSCs,withoutdetection of alpha-fetoprotein(AFP).Conclusion The optimization of rats' age,the density of Percoll,the methods of cell cultivation are conducing to obtaining a pure population of MSCs with good differential activity.MSCs are inclined to differentiate into mature hepatocyte-like cells under the induction of HGF/FGF-4.

Objective To set up a successful after service response system for medical equipment. Methods After service response system for medical equipment company was further studied by using model analysis method. Results It was demonstrated that medical equipment companies must improve the after service response system. After service strategies were made according to the requirement of clients and the strategies were embodied in the response system. Conclusion The market competitive power of medical equipment companies are raised.

Objective: To simultaneously detect five components (phillyrin, chlorogenic acid, chlorpheniramine maleate, vitamin C and acetaminophen) in compound paracetamol vitamin C Yinqiao tablets.Methods: Using a Waters Xselect C18 column(250 mm×4.6 mm, 5 μm), the mobile phase consisted of acetonitril-0.1 mol·L-1 NaH2PO4(pH 3.0) with gradient elution at a flow rate of 1.0 ml·min-1.The detection wavelength was 230 nm and the column temperature was 35℃.Results: The linear range of vitamin C, acetaminophen, chlorogenic acid, chlorphenamine maleate and phillyrin was 0.500-200.200, 0.499-199.600, 0.501-200.100, 0.502-200.900 and 0.488-195.400 μg·ml-1(r＞0.999), respectively.The average recovery was 99.3%(RSD=1.5%), 97.8%(RSD=1.7%), 98.5%(RSD=1.1%), 97.1%(RSD=1.7%)and 99.8%(RSD=1.2%)(n=9), respectively.Conclusion: The simple and sensitive method can be applied in the quality control of compound paracetamol vitamin C Yinqiao tablets.

Convulsive status epilepticus (CSE) is an acute,life-threatening and neurologic emergency in children.The prognosis and the incidence of recurrent CSE depend upon the underlying etiology.It is very important to find related etiology and seek solution.The underlying etiology,classification and the diagnostic evaluation of the children with CSE will be outlined in this article.

Objective To explore dental phobia in children 's oral treatment sevoflurane inhalation anesthesia clinical results.Methods 100 cases of dental phobia children in our hospital between January 2012 to January 2014 were observed, which were divided to the school -age group and preschool group , 50 patients, respectively.They were all treated with heptafluorobutyric alkyl inhalation anesthesia , and the anesthesia effects were compared .Results The adverse effects, oxygen saturation and mean arterial blood pressure changes and induction of anesthesia in chil -dren awake have statistically significant difference (p <0.05).Conclusion This study suggest that sevoflurane in -halation anesthesia is an effective dental phobia children 's oral therapy, and has a high application value .

In the last few years, Many medical colleges have been joined with synthetic universities because of creat-ing the international and well-known and high level universities. In the article the author expounds the exploitation'sadvantage of synthetic university respectively from the foundational mode, the humanities and agency science, the cal-culator technique and the knowledge diversification. He also discusses the problems arising from the united universityand the correlative plan to the effect of the problems from the contradiction of resources` division,the managing mode ofthe medical education, the phenomena of "Teaching is heavier than Researching" and the popularization of highereducation, hoping to draw lessons from the advanced experience abroad extensively to enhance and perfect the newmode of our higher medical education.[

To adapt to the variety of environment,the teaching of clinical practice must face some innovations and reformations in the contents and methods.The article discusses the specialties and applications of the several main resources,such as clinical teachers(the resource of manpower)?the training center of clinical skills(the resource of methods) ?the SP(the resource of carriers),then,provides some useful information for the teaching innovations in clinical practice.

Medical English words have their own features in word formation.The medical terms are in huge sum but meanwhile of extremely typical characteristics.This paper tries to help the medical students learn these terms more efficiently by analyzing the characteristics of these terms.

There exist insufficient quantity,irregular quality,insufficient innovation ability in the existing economy and trade talents of medicine in our country,which is far from satisfying the need of the market.With the development of medicine economy,economy and trade talents of medicine become the focal point that medicine market economy competes for.As a cradle of training medicine persons,universities of medicine are confronted with the pattern reformation of innovative personnel training.The article brings forward countermeasures from these aspects: innovative idea,personnel training pattern,contents of courses and teaching methods and innovative teacher quality.