Objective To investigate the expression and implication of p21~(WAF1) and Ki-67 in the le- sions of Bowen Disease.Methods p21~(WAF1),Ki-67 proteins were examined with immunohistochemical SP method in 35 cases Bowen disease and 12 cases of normal skin or muscosa.Results Both of the expression of p21~(WAF1) protein and Ki-67 protein were elevated in Bowen disease lesions(both P

Hepatocyte growth factor(HGF) is a mesenchyme-derived pleiotropic factor which regulates cell growth,cell motility,and morphogenesis of various types of cells,and is thus considered a humoral mediator of epithelial-mesenchymal interactions responsible for angiogenesis,apoptosis and oxidative stress,etc.This article reviewed the relationships between HGF and cardiovascular diseases.

Child ; Female ; Hearing Loss, Bilateral ; etiology ; Humans ; Mumps ; complications

Objective:By observing effects of Dahuang Zhechong Pills on CD44,VEGF and the metastasis suppressor in mice with Lewis Lung carcinoma,to discuss its mechanism of anti-tumor metastasis preliminarily.Methods:The mice were divided randomly into five groups the day after modeling and each group included ten mice.According to the pre-test,high-dose,medium-dose and low-dose were quantitated with 0.18g/ml,0.09 g/ml,0.045g/ml.The model group was intragastric administrated with NS 0.2ml for ten days;the chemotherapy group was Intraperitoneal administrated 50mg/kg CTX on the first day.Observing and numerating the pulmonary metastases with nodules by pulmonary pressure slide film on the 12th day.Based on immunohistochemical method to detect the expression of VEGF and the activity detection by flow cytometry CD44,Its anti-tumor mechanism and the role of target were preliminarily studied.Results:The inhibition rates of lung spontaneous metastasis in experimental groups of high(H),medium(M),low(L)dosages and the chemotherapy group were respectively 57.44 percent, 55.32 percent,38.72 percent,60.00 percent.CD44,respectively,values were 0.59?0.86,0.21?0.28,0.33?0.31,1.36?0.52; the experimental group(M)compared with model group,VEGF expression was significantly reduced,there was a significant difference(P0.05).Conclusion:Dahuang Zhechong Pills reduced the lung metastases incidence of C57BL mice with Lewis lung carcinoma and prolonged their lives.These results were obvious in the medium-dose experimental group.Medium-dose of Dahuang Zhechong Pills had effects on C57BL mice with Lewis lung carcinoma,whose CD44 activity were inhibited and the expression of VEGF was reduced.

Based on their multiple years of personal experiences in hospital administration,authors of this article point out that medical service,humanistic service,and emotional service should be the core aspects in the current hospital competition.Medical professionalism embodied in the three levels of consciousness,behaviors,and regulations should be the main subject in the research on the role of medical ethics in hospital administration and medical treatment behaviors.It is an urgent ethical requirement and the chief task for hospital administrators to achieve humanistic medical service through humanistic administration of hospitals.

Angiofibroma ; metabolism ; pathology ; surgery ; Angiomyoma ; metabolism ; pathology ; Antigens, CD34 ; metabolism ; Diagnosis, Differential ; Female ; Fibroma ; metabolism ; pathology ; Follow-Up Studies ; Humans ; Leiomyoma ; metabolism ; pathology ; Middle Aged ; Mucin-1 ; metabolism ; Myxoma ; metabolism ; pathology ; Vimentin ; metabolism ; Vulvar Neoplasms ; metabolism ; pathology ; surgery

OBJECTIVETo study the effect of aqueous extract of Taxus chinensis var. mairei (AETC) combined Erlotnib on the growth of A549 xenograft in nude mice and its mechanism.

METHODSThe xenograft model in nude mice was established by inoculating A549 cells subcutaneously. BALB/c nude mice bearing A549 xenograft were randomly divided into six groups, i.e., the low dose Erlotinib group (A) , the standard dose Erlotnib group (B) , the low dose Erlotinib combined AETC group (C), the standard dose Erlotnib combined AETC group (D), the AETC group (E), the control group (F), 12 in each group. Different medication was performed for 7 successive weeks after 24 h. One mL blood was withdrawn and tumor tissues taken. The tumor inhibition rate was calculated. The combined effect was analyzed by Jin's Formula [Q = Ea + b/(Ea + Eb-Ea x Eb) ]. mRNA and protein expression levels of epidermal growth factor receptor (EGFR), cyclooxygenase-2 (COX-2), and B cell lymphoma-2 (Bcl-2) in xenografts were detected using real-time RT-PCR and ELISA.

RESULTSCompared with Group F, the xenograft weight was obviously lowered in Group B-E (P < 0.05, P < 0.01). The q value was 0.92 in Group C and 0.96 in Group D, which was obtained by simple adding of the two drugs. Compared with Group F, EG- FR mRNA expression in Group D and E, COX-2 mRNA expression in Group A-E; Bcl-2 mRNA expression in Group B-D; COX-2 protein expression in Group B-E; Bcl-2 protein expression in Group C and D were obviously lowered with statistical difference (P < 0.05, P < 0.01).

CONCLUSIONSAETC combined low dose and standard dose Erlotinib had synergistic effect on tumor inhibition. Its mechanism might be associated with down-regulating mRNA and protein expression levels of COX-2 and Bcl-2.

Animals ; Antineoplastic Agents ; pharmacology ; Antineoplastic Combined Chemotherapy Protocols ; pharmacology ; Cell Line, Tumor ; Cyclooxygenase 2 ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Enzyme Inhibitors ; pharmacology ; Erlotinib Hydrochloride ; pharmacology ; Heterografts ; Lung Neoplasms ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Receptor, Epidermal Growth Factor ; metabolism ; Taxus ; Transplantation, Heterologous

We summarized our accumulated clinical and teaching experiences and explored the regularity of acupuncture needling and teaching. It is of great importance in pressing hand during inserting needle. Stroking and pressing are two crucial parts which deserve more attention, and seldom useage of pressing hand should be abolished. Operating hand needs practice before inserting needle, while it should fully relaxed during inserting. Blending "touching", "stretch" "gathering" "erupting" and "advancing" in single moment, applying appropriate dynamic mode of inserting needle such as "join 3 forces as one" "3 points in a line" expertly and naturally. In addition, enough attention should be paid on "altering direction" and "shifting point". Inserting deftly and powerfully, no/slight sensation, deqi when inserting needle are the highest reflection as an acupuncturist.
Acupuncture ; education ; Acupuncture Therapy ; instrumentation ; methods ; China ; Humans ; Teaching

Burnout, Professional ; psychology ; Faculty ; Female ; Humans ; Male ; Mental Health

Objective To explore the effect of brain derived neurotrophic factor(BDNF) on the birth weight and the brain weight of newborn rats with intrauterine growth retardation(IUGR).Methods Female and male Sprague-Dawley rats were mated together and the vaginal plug formation after mating was for pregnancy diagnosis.The day when the plug was observed was considered day 1 of gestation.Then,the pregnant rats were divided into IUGR group and sham surgery group.On day 17 of gestation,the uterine arteries of pregnant rats in IUGR group were clamped for 30 min by blood vessel forceps after abdominal cavity with clamping blood uessels was opened.The pegnant rats in sham surgery group only had abdominal cavity opened without clamping blood vessels.Then the pegnant rats in IUGR group received 9 g/L saline(IUGR group) or BDNF 1 ?g injected in the rat through caudal veins for 5 days after surgery(BDNF intervention group).After vaginal birth,the weight of newborn rats and their brain weigh were measured and analyzed.Results Compared with the sham surgery group,the birth weight and brain weight of IUGR group group rats decreased by 24.9% and 17.3%,respectively,the results were significant(Pa