AIM: Clarification of sanchi(Panax notoginseng(Burk.)F.H.Chen) extract by ceramic microfiltration membrane was studied. METHODS: Different operating parameters on membrane flux were investigated.Membrane was cleaned by using strong base and strong acid.The effective compound of ginsenoside R_(g1) and notoginsenoside R_1 before and after microfiltration were studied. RESULTS: The sanchi extract became clear after microfiltration.The removal efficency of the whole solid was 31.3%,the metastasis rate of the effective ingredient was 84.8%,and the growth rate of the effective ingredient was 23.5%. CONCLUSION: The ceramic microfiltration technique is a feasible new one for clarification of sanchi extract.

Objective: To measure the convergence angles of teeth in different teeth positions and different denture designs.Methods:Mesio-distal widths and labio-lingual widths were measured on 226 teeth according to three points in a line on three surfaces (gingival, middle and incise) of each tooth.The distance between every point and the counterpart point in the adjacent surface was measured.Then convergence angles were calculated. Results:The means of the angles were 4.13°±0.77°,4.14°±0.76°, 4.46°±0.95°, 4.76°±0.84° on the first and second premolar, the first and second molar,respectively . There were significant differences between the premolars and molars(P<0.05).On the dentures with the designs of monocrown, triunit-bridge and long bridge the means of the angles were 3.78°±0.74°,4.69°±0.75° and 5.08°±0.85° respectively,the angle on the denture with the design of monocrown was smaller than that of bridge (P<0.05).Conclusion:The convergence angles are different according to different teeth positions and different denture designs, so they should be adjusted according to the clinical requirements.

Objective To explore the effect of Cyclin A on origin recognition complex-1 (ORC1) expression of rat vascular smooth cells (VSMCs).Methods Primary VSMCs of thoracic aorta in rats was obtained by the adherence method of tissue culture.The synchrony of cell was obtained by the method of double-thymidine block.In different cell cycles of VSMCs,the expression of ORC1 mRNA was determined by RT-PCR and the protein expression of ORC1 was observed by flow cytometry.Results Synchronized VSMCs were obtained and identified by the methods of double-thymidine block,colchicine treatment and serum starvation.Synchronized growth was monitored by flow cytometry.All the synchronized VSMC's distribution ration was (89.22±3.54) ％ at G0/G1 phase,(66.74 ±7.16)％ at G1/S phase,(63.24 ±4.06)％ at S phase and (51.64 ± 11.18)％ at G2/M phase and there was statistically significant difference compared with other phase(P ＜0.01).There was no significant effect of Cyclin A on ORC1 mRNA expression at a quiescent stage of VSMC.At G2/M phase peaked ORC1 was (52.133 3 ± 2.122 1)％,at G1/S phase was(10.916 7 ± 0.531 1)％,at S phase was (7.656 7 ± 0.412 4)％,and there was statistically significant difference (P ＜0.01).At G2/M phase ORC1 downed to the lowest point was (1.276 7 ± 0.161 7) ％,at G1/S phase was (13.371 0 ± 1.057 3)％,at S phase was (3.043 3 ± 0.538 0)％,and there was statistically significant difference (P ＜ 0.01),suggesting that Cyclin A might prevent ORC1 binding the chromatin of VSMCs.Conclusion Cyclin A may be an important regulative factor at the initiation of ORC1 in VSMCs.

Objective To investigate the long-term efficacy of calcium phosphate cement combined with bFGF in repairing pulp chamber perforation and to analyze the correlation between the diameter of the perforation and the curative effect.Methods 75 patients with pulp chamber perforation (82 teeth) were enrolled and divided into the observation group and the control group according to the repair material and method.A series of subgroups were also modeled according to the diameter of the perforation,which include the control group A (≤ 1.5 mm),control group B (1.6～3 mm) and control group C (＞3 mm),as well as the observation group A (≤ 1.5 mm),observation group B (1.6～3 rmm) and observation group C (＞3 mm).The observation group was treated with calcium phosphate cement combined with bFGF,and the control group was treated with calcium phosphate cement alone.Results The total effective rate of the observation group was 97.8％,which was significant higher than 80.6％ in the control group (P＜0.05).The cure rate of the observation group A was 100％,which was significant higher than 73.3％ in the observation group B and 41.7％ in the observation group C (all P＜0.05).The total effective rates of the observation group A and B were significantly higher than 91.7％ in the observation group C (all P＜0.05).The cure rate of the control group A was 92.9％,which was significant higher than 60.0％ in the control group B and 25.0％ in the control group C (all P＜0.05).The total effective rates of the control group A (100％) and B (90.0％) were significantly higher than 91.7％ in the control group C (all P＜0.05).Conclusions Calcium phosphate cement combined with bFGF in repairing the pulp chamber perforation was significantly better than calcium phosphate cement alone.The cure rate of perforation repairing is closely related to the perforation size.The perforation with small diameter may achieve a better repairing effect.

Objective:To culture human alveolar osteoblasts and study its osteogenic characteristics in vitro.Methods:Human alveolar bone was obtained from human donor and cultured in explants.Cells migrating from explants were observed by inverted phase-contrast microscope and cell proliferation was detected by MTT assay.After cultured in conditional medium containing dexamethasone,?-glycerphosphoric sodium and ascorbic acid,cells were tested by ALP assay,Alizarin red assay and von Kossa assay,3H-proline labeling with collagenase digestion method.Results:The alveolar osteoblasts migrated from explants after 5 d culture.Cells could be passaged after 14-16 d culture.ALP values were obviously positive and cells showed positive reaction by Alizarin red assay and von Kossa assay in conditional medium.The cultured human alveolar osteoblasts secreted type I Collagen.Conclusion:The human alveolar osteoblasts cultured in this experiment grow well,and the morphological and biological characteristics of the culture cells are similar to those of osteoblasts.

Humans ; Male ; Prostate ; anatomy & histology ; pathology ; Prostatic Diseases ; pathology ; Prostatic Hyperplasia ; pathology

Objective To study the effect of mannose receptor(MR) from human spermatozoa on sperm-egg fusion. Methods The Balb/c mouse was immunized with the purified mannose receptor(pMR) which was isolated from motile human sperm by mannose-agarose gel affinity chromatography and the anti-MR serum was harvested. Human sperm after induction of acrosome reaction was pretreated with anti-MR serum and subsequently processed in sperm penetration assay (SPA). Results The anti-MR serum can inhibit human spermatozoa from binding and penetrating zona-free golden hamster eggs with a dose-dependent reduction of Fertilization rate(FR) and Penetration index(PI).Conclusion The results indicate that MR of human sperm plays an important role in sperm-oocyte fusion.

hyperflexion,and the differentiation was significant(P

Background and purpose:Twist has been identifi ed as tumor metastasis promoter transcription factor and KiSS-1 has been identified as tumor metastasis suppressor gene,and both of them have been identified to be associated with the metastatic potential of non-small cell lung cancer(NSCLC) .Our aim was to identify the expression of both Twist and KiSS-1 in NSCLC and analyze their correlation with patients’ survival.Methods:Immunohistochemical staining using monoclonal Twist and KiSS-1 antibody were performed on paraffi n embedded specimens from 61 patients diagnosed with NSCLC,and 15 specimens of tumor surrounding lung tissue were used as control.The association with clinicopathologic data and prognosis of NSCLC were analyzed.Results:The expression of Twist was significantly higher in NSCLC than in tumor surrounding lung tissue(P

Objective To explore origin recognition complex 1(ORC1) expression in the rat vascular muscle cells at different phases of proliferation.Methods Vascular smooth muscle cells(VSMCs) of thoracic aorta of rats in primary culture were obtained by the adherence method of tissue culture.Total RNA of VSMCs was extracted.The expression of ORC1 mRNA of VSMCs at different phases of proliferation was determined by reverse transcription polymerase chain reaction(RT-PCR) and the expression of ORC1 protein by immunocytochemistry and laser confocal microscopy.Results Cultured VSMCs were confirmed by light microscope and immunocytochemistry.The expression of ORC1 mRNA in the quiescence stage of VSMCs was not found.After VSMCs were stimulated with serum,the level of ORC1mRNA had an obvious increase at 6 h,peaked at 12 to 24 h and decreased in the following 24 h.The expression of ORC1 protein was also not found in the quiescence stage of VSMCs,but the level of ORC1 protein during proliferation of VSMCs was significantly increased.Conclusion ORC1 may have an important role during the process of VSMCs proliferation in rats.