Adult ; Cervical Intraepithelial Neoplasia ; diagnosis ; surgery ; Electrosurgery ; methods ; Female ; Humans ; Middle Aged ; Uterine Cervical Neoplasms ; diagnosis ; surgery

Objective To determine the content of astragaloside Ⅳin Qi Zao Granules.Methods Equipped with the evaporative light scattering detector (ELSD), a RP-HPLC method was established. The chromatographic column was C18 and the column temperature was at 30 ℃.The drift tube temperature was at 40 ℃and the evaporative gas was nitrogen with pressure of 3.5 bar.The mobile phase was acetonitrile-water(30∶70) and the flow rate was 1mL/min. Results This method showed a good linear relationship in the range of 2.51～50.2 ?g astragaloside Ⅳ. The average recovery was 98.13 %with RSD being 1.28 %. Conclusion The method is sensitive and accurate and can be used for the quality control of Qi Zao Granules.

Background & Objective: Early life stresses, such as social isolation, have lasting effects on the development of emotion and behavior, in which vasopressin plays important roles. This study aimed to assess the possible association of central release of vasopressin with social isolation. Methods: The social isolation model was performed in male mice who endured 6-week social isolation after weaning. Vasopressin expression in the paraventricular nucleus of hypothalamus (PVN) was measured with immunohistochemistry. Released vasopressin from PVN was measured with radioimmunoassay. Results: Vasopressin immunoreactive cells number decreased in the PVN, medial parvocellular division in social isolation-reared mice, compared to the group-reared counterparts. Social isolation decreased short social exposure-induced vasopressin release from PVN. Isolation-reared mice exhibited anxiogenic profile and difficulty in social recognition. Conclusions: This study provides new evidence for the important role of vasopressin in the development of emotional and social behaviors.

BACKGROUND:Bioimpedance method can be used to extract physiology and pathology information relative to the gastricmotility states,to investigate the courses of contraction,peristalsis,transmission and emptying of the stomach,to measure andevaluate gastric motility function.However,early studies mainly focused on the emptying of the stomach,and few studies onevaluation of gastric motility by extracting gastric motility information.OBJECTIVE:To understand the gastric motility from electricity-mechanism composite concept and to establish a noninvasive,convenience measurement method of gastric motility impedance.METHODS:Based on the characters of gastric motility impedance signal,a measurement method of impedance gastric motility isintroduced.Some foundation experiments about the gastric emptying measurement,the gastric motility compare between fastingand repast,the gastric motility comparison of healthy volunteers during different periods and some medicine effects on gastricmotility have carried out.The gastric motility measurement of the patients with functional dyspepsia and gastric motility evaluationfor the patients with erosive gastritis had been reported.RESULTS AND CONCLUSION:Using electrical bioimpedance method,according to the electrical property of the stomach tissueor the food in stomach and the change rules of the property,the physiology and pathology information relative to the gastricmotility states can be extracted,the courses of contraction,peristalsis,transmission and emptying of the stomach can beinvestigated and noninvasive measure and evaluation of gastric motility function can be realized.

Objective To observe the effects of Taizhian treatment on macrophages expression in the plaque of aotic intima and blood lipid levels in hypercholesterolemic rabbits. Methods Sixteen male New Zealand rabbits were given 1%high-cholesterol diet. After 8 weeks,these macrophages expression in aotic intima was detected by immunohistochemistry. Results Compared with normal rabbits,Taizhian treatment decreased the expression of macrophages in aortic intima by 25%and reduced serum levels of low density lipoprotein-cholesterol by 21%in hypercholesterolemic rabbits(both P<0.05). Conclusions Taizhian may significantly decrease blood lipid level and reduce atherosclerosis in hypercholesterolemic rabbits.

Objective To explore the therapeutic efficacy of posterior internal fixation and anterior debride-ment with autogenous bone grafts at one stage on tuberculosis of thoracic or lumbar spine . Methods 16 cases of thoracic or lumbar spinal tuberculosis patients were treated with combined anterior (radical debridement and bone autograft) and posterior (instrumentation) surgeries in one stage between September 2003 and September 2007. The degree of the kyphosis (Cobb angle) was measured and the interbody fusion was observed preoperatively and postop-eratively. The ASIA grading system was used to assess the neurological status. Results All patients were followed up for 10 months to 36 months,on average of 12 months. All patients showed sucessful interbedy fusion,but Cobb angle was not progressed. No recurrence or wound infection was found. 6 cases all got nerve function recovery. Conclusion Thoracic or lumbar tuberculosis treated with this surgical technique can achieve stable internal fixation and a high satisfactory rate with restoring the spinal stability, arresting the disease early, providing early fusion, correcting the ky-pbosis particularly.

Objective To establish a quantitative analysis of multi-components by single-marker (QAMS) for determination of four components (adenosine, uridine, guanosine, (R, S)-goitrin) in Isatidis Radix granules.Method The QAMS for Isatidis Radix granules was established and validated and adenosine was selected as the internal reference substance.The relative correction factor (RCF) of other three components were calculated.The contents of four components were determined by both external standard method and QAMS.The QAMS method was validated through comparison of the results obtained by the two different methods.Results The RCFs of uridine, guanosine and (R, S)-goitrin all showed good reproducibility within certain ranges.For Isatidis Radix granules, uridine, guanosine and (R, S)-goitrin, there was no significant difference between the quantitative results of the two methods.Conclusions The QAMS method is simple, feasible and credible to be used for comprehensive quality control of Isatidis Radix granules.

Objective To evaluate the effects of pulsed ultrasound at different intensities on the healing of standardized contusions in an animal model. Methods Forty-eight 3-month-old, male Sprague-Dawley rats were given experimental contusions of the right gastrocnemius muscle before being divided into four groups randomly ( n =12 in each group): (1) a muscle injury control group (muscle injury without treatment); (2) a muscle injury and pulsed ultrasound (0.25 W/cm2 ) group; (3) a muscle injury and pulsed ultrasound ( 0.5W/cm2 ) group; and (4)a muscle injury and pulsed ultrasound ( 0.75 W/cm2 ) group. Pulsed ultrasound treatment ( frequency 3 mega Hz)was started 24 hours post injury and delivered 5 min daily for 14 days on the injured right hindlimb. At days 4, 7 and 14 after injury, muscle samples were analyzed through hematoxylin-eosin staining and immunohistochemistry for the detection of muscle satellite cells and desmin. Results The average optical density (IOD) of desmin-positive mononucleated cells had increased significantly at days 4, 7 and 14 post injury in the treatment groups compared to the control group, but with no statistically significant difference among the 3 ultrasound treatment groups. Conclusions The pulsed ultrasound treatment played a beneficial role in skeletal muscle regeneration after contusion. There was no significant dose-dependent effect over the intensity range of 0.25-0.75 W/cm2.

OBJECTIVE:To discuss ward drug control to ensure safe.METHODS:We analyzed retrospectively the application of 5S theory in ward drug control.RESULTS & CONCLUSION:By this drug control mode,drugs were classified according to varieties,usage,frequency of use etc,drug control quality and work efficiency were enhanced,patients' needs could be satisfies and staff' s professional quality was enhanced.