Objective To evaluate the feasibility of endoscopic thyroidectomy via an incision under the armpit. Methods A skin incision was made under the armpit and a channel was established to thyroid gland and endoscopic thyroidectomy was performed in 32 cases suffering from unilateral thyroid lesion including 24 cases of solitary adenoma, 5 cases of multiple adenoma, 3 cases of cystic goiter. Results The average operative time was 125 min and the average blood loss was 55 ml. There were not any postoperative complications. Conclusion Endoscopic thyroidectomy is a mini-invasive surgery. The operative field was clear and ultrasonic scalpel is capable of dealing with blood vessels during the procedure.

Objective To reduce the risk of blood transfusion ,and discuss the serological characteristic and cross‐match test of those patients who got positive both in autoantibody test and direct coombs test .Methods With absorption‐elution testing ,antibody screening cells and panel cells belong to different manufactures and batch numbers were applied to distinguish autoantibody from al‐loantibody within serum and reagent red cells for absorption test .Appropriate donors were selected to do cross matching test ;the specificity of autoantibody and alloantibody and the relationship between ABO blood type ,diseases ,anemic and the efficacy of blood transfusion were analysed .Results Among the 139 study subjects ,all of them were identified positive both in autoantibody and di‐rect antibody test ,including 20 cases just have autoantibody ,59 cases accompanied with Rh system or MNs system or Kidd system antibody ,47 with autoantibody except for alloantibody ,13 with drug resistant or other system antibody .Within the 221 cases of blood transfusion ,none of them has hemolytic transfusion reaction .We found a positive correlation(P<0 .05)between the intensity of agglutination of autoantibody and direct antibody and anima ,while a negative correlation(P<0 .05)with the efficacy of transfu‐sion .Similarly ,there is a positive correlation(P<0 .05)between the intensity of autoantibody and the sensitization of autologous e‐rythrocyte ,most of which are immunologic diseases ,while has no correlation with ABO blood type(P>0 .05) .Conclusion To those cases which were identified positive both in autoantibody and direct antibody test ,the degree of anaemia ,the numbers of transfusion and the efficacy of transfusion were associated with the intensity of agglutination of autoantibody and direct antibody .In order to de‐crease the risk of blood transfusion and make it highly efficient ,we should affirmed it if there is any autoantibody .In this process , an appropriate procedure and assay must be adopted .Besides ,relative antigen‐negative donors or high frequency local autoantibody need to be matched with corresponding the donors who have the same type of antigen .

Objective To identify a new miRNA combination that could be used as a diagnostic marker for acute myocardial infarction (AMI) or acute cerebral infarction (ACI).Methods We surveyed the literatures and databases and selected miRNAs with a high frequency in AMI or ACI as candidate genes.We collected peripheral blood samples from 13 patients with AMI,11 patients with ACI,and 20 healthy volunteers.RNA was extracted from these samples,and gene expression levels were determined by reverse transcription and real-time quantitative PCR.The differences in the expression of the candidate miRNAs were then analyzed.Results The frequencies of miRNA1,miRNA499a,and miRNA 133a were higher in the AMI group,whereas the frequencies of let7i,miRNA16,and miRNA223 were higher in the ACI group.These six miRNAs were significantly higher in the two disease groups than in the control group (P ＜ 0.01).The levels of miRNA1 and miRNA499a were 4.82-and 3.50-times higher in the AMI group,respectively (both P ＜ 0.01),when compared with the ACI group.The levels of let7i in the ACI group were 2.61-times higher than that in the AMI group (P ＜ 0.05).There were no significant differences in the expression levels of miRNA 133a,miRNA16,and miR-NA233 between the AMI and ACI groups (P ＞ 0.05).Conclusion The abnormal increase in six miRNAs in human peripheral blood could be used as a marker for the diagnosis of AMI or ACI.The expression levels of miRNA 1 and miRNA499a were 11-to 20-times higher than control levels,and the expression levels of let7i were 6-times higher than control levels,which could be used to predict the risk of AMI and ACI,respectively.