ObjectiveTo investigate the clinical effect,suitable dose,and its safety of dezocine combined with propofol used in painless artificial abortion.MethodsWith a completely randomized double blind method,200 cases of ASA Ⅰ or Ⅱ early intrauterine pregnancy patients were divided into A,B,C,and D four groups ( n =50).Each patient in A,B,and C groups was infused dezocine in 0.10,0.15,and 0.20 mg/Kg,respectively.D group was administrated with intravenous fentanyl 1.5 μ g/kg,then 3min later,slowly intravenous injection of 1％ of propofol.MAP,HR,RR,and SpO2 were recorded at the before anesthesia induction (T0 ),loss of eyelash reflex time (T1 ),recovery time (T2),and recovery 10 -min time (T3 ).Meanwhile,the parameters including the anesthesia induction time,anesthesia recovery time,directional force recovery time,induction dosage and total dosage of propofol,and the intraoperative and postoperative analgesic efficacy and adverse reaction were compared among different groups.Results For the A and D groups,MAP,HR,RR,and SpO2 at the T1 were all lower than that at the T0 ( P ＜0.05).For the B group,MAP and HR at the T1 were lower than that at the T0 ( P ＜ 0.05).The anesthesia recovery time and orientation recovery time in the B and C groups were significantly shorter than A and D groups ( P ＜ 0.05 ),and significantly statistical difference was found between A and D groups,no statistical difference between B and C groups ( P ＞ 0.05).The propofol induction dosage and total dosage were different among groups ( groups C ＜ B ＜ D ＜ A) with a statistical significance ( P ＜ 0.05 ).The intraoperarive analgesia effect,postoperative pain score grading,and intraoperative body dynamic reaction rates in the B and C groups were significantly different from the A and D groups ( P ＜ 0.05 ),and no statistical significance was found between B and C groups ( P ＞ 0.05 ).The respiratory inhibition,postoperative nausea,vomiting and other adverse reaction rates in the B and C groups were significantly lower than those in A and D groups ( P ＜ 0.05),and those parameters in B group were lower than C group.ConclusionsDezocine combined with propofol is definitely effective and very safe in painless induced abortion with very less respiratory inhibition,nausea,vomiting,and other adverse reactions; and their optimal dosage is 0.15 mg/kg.

Objective To investigate the effects of Rosa Laevigata Michx Flavoid( RLMF) and Rosa laevigata Michx Polysaccharose (RLMP) on expression of TRPV5 in IgA Nephropathy (IgAN) rat renal tissue. Methods Forty Sprague-Dawley rats were randomly assigned to four groups.The rat model of IgA nephropathy was induced by intragastric administration of bovine serumalbumin and injections of LPS and CC14.Eight weeks later,the rats with IgAN were treated with RLMF or RLMP (4 weeks), or normal saline.Rats was sacrificed at thirteenth weeks, and RNA was extracted from the kidney.Expression of TRPV5 in tubulointerstitial tissues were analyzed by fluorescent quantitative RT-PCR. Results After RFLP intervention,the expression levels of TRPV5 were markedly increased (P<0.01) than model control group,while decreased (P<0.05) than normal control group but had no significance with model control group after RFLF intervention. Conclusion TRPV5 expression is decreased in IgAN,and RLMP can adjust TRPV5 expression and improve renal function of IgAN.

This study examined the gene expression patterns of peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE) by using serial analysis of gene expression (SAGE) technology. Following the construction of serial analysis of gene expression (SAGE) library of PBMCs collected from 3 cases of familial SLE patients, a large scale of tag sequencing was performed. The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software. The top 30 expressed genes of SLE patients were uploaded to http://david.niaid.nih.gov/david/ease.htm and the functional classification of genes was obtained. The differences among those expressed gene were analyzed by Chi-square tests. The results showed that a total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags. Among the 1286 unique tags, 86.8% had single copy, and only 0.2% tags had more than 20 copies. And 68.4% of the tags matched known expressed sequences, 41.1% of which matched more than one known expressed sequence. About 31.6% of the tags had no match and could represent potentially novel genes. Approximately one third of the top 30 genes were ribosomal protein, and the rest were genes related to metabolism or with unknown functions. Eight tags were found to express differentially in SAGE library of SLE patients. This study draws a profile of gene expression patterns of PBMCs in patients with SLE. Comparison of SAGE database from PBMCs between normal individuals and SLE patients will help us to better understand the pathogenesis of SLE.
Expressed Sequence Tags ; Gene Expression Profiling/*methods ; Leukocytes, Mononuclear/*metabolism ; Lupus Erythematosus, Systemic/blood ; Lupus Erythematosus, Systemic/*genetics ; Sequence Tagged Sites ; Transcription, Genetic

This study examined the gene expression patterns of peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE) by using serial analysis of gene expression (SAGE) technology. Following the construction of serial analysis of gene expression (SAGE) library of PBMCs collected from 3 cases of familial SLE patients, a large scale of tag sequencing was performed. The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software.The top 30 expressed genes of SLE patients were uploaded to http://david.niaid.nih. gov/david/ease.htm and the functional classification of genes was obtained. The differences among those expressed gene were analyzed by Chi-square tests. The results showed that a total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags. Among the 1286 unique tags, 86.8% had single copy, and only 0.2% tags had more than 20 copies. And 68.4% of the tags matched known expressed sequences, 41.1% of which matched more than one known expressed sequence. About 31.6% of the tags had no match and could represent potentially novel genes. Approximately one third of the top 30 genes were ribosomal protein, and the rest were genes related to metabolism or with unknown functions. Eight tags were found to express differentially in SAGE library of SLE patients. This study draws a profile of gene expression patterns of PBMCs in patients with SLE. Comparison of SAGE database from PBMCs between normal individuals and SLE patients will help us to better understand the pathogenesis of SLE.