1.Humanity Education in the Teaching of Epidemiology
Xialing JIANG ; Shouyi YU ; Qing CHENG
Chinese Journal of Medical Education Research 2006;0(12):-
During the teaching process,the humanity thought was infiltrated into the professional knowledge so as to cultivate the students'humanity thinking and healthy personality.
2.Protsome analysis of the total protein in Vibrio cholerae by 2-DE
Shouyi CHENG ; Junhua LIU ; Xinqiang ZHANG ; Yushan HU ; Chuanhua LI ; Ming WANG
Chinese Journal of Microbiology and Immunology 2008;28(11):963-966
Objective To study the different proteins of human and environment Vibrio cholerae (V. cholerae) by two dimensional electrophoresis (2-DE) and TOF-TOF-MS. Methods Total cellular pro-tein was extracted by lysate from V. cholerae, and proteins were separated by 2-DE under immobilized pH gradients(IPG), then electrophoregrams were dealed with coomassie brilliant blue, and analyzed by Im-ageMaster 2D Elite 5.0, finally the different protein spots were identified by TOF-TOF-MS. Results High repetitive 2-DE maps were obtained. 2-DE and image analysis revealed 1032±22 protein spots, and PI value was among 4.00 to 7.20. Matching spots were 1025±24 in two repeats electrophoregrams and matching ratio was 96.30%. Conclusion The different protein spots were successfully established with high quality and sharpness separation by 2-DE and TOF-TOF-MS, which stands as a valuable resource for proteomics re-search of V. cholerae.