Objective To recognize the ability to identify and validate N .gonorrhoeae in Zhuhai STD laboratories to timely find out the factors or problems influencing the results.Methods Samples were uniformly delivered to STD laboratories in 2011~2014.The laboratories identified and validated N .gonorrhoeae and PPNG.Test results were analyzed and evaluation results were fed back to participating laboratories.Results 220 samples were delivered to STD laboratories in four years. The average percentage of qualified labs was 88.6% (39/44).The coincidence rates for N .gonorrhoeae quality control sam-ples respectively were 78.8% (26/33),90.3% (28/31),87.9% (29/33)and 100% (33/33)in 2011 ~ 2014.The coinci-dence rates for PPNG respectively were 80.8% (21/26),78.6% (22/28),86.2% (25/29)and 100% (33/33).Conclusion Through the construction of STD standardization laboratory and the external quality assessment every year,the STD labora-tories of Zhuhai area improved the ability to validate and identify N .gonorrhoeae obviously.But several laboratories had some problem,such as careless technicians and inaccurate reports.So quality control should be still strengthened.