1.Investigation on degradation process of collagens during manufacturing procedure of E'jiao.
Guifeng ZHANG ; Tao LIU ; Qian WANG ; Jian LUO ; Ru XIE ; Yufeng QIN ; Jinhua YOU ; Shousheng TIAN ; Zhiguo SU
China Journal of Chinese Materia Medica 2009;34(10):1211-1215
OBJECTIVETo investigate the degradation process of collagens and identify the key unit operation during manufacturing process of E'jiao.
METHODSamples in different unit operations were withdrawn, and their amino acid compositions and the molecular weight ranges were determined. The peptide composition was analyzed by high-performance liquid chromatography/mass spectrometry.
RESULTThe content of sample during atmospheric condensation unit increased by 16.8% compared to the thermal extraction unit. Gel filtration chromatographic analysis indicated that the degradation process of collagen primarily occurred during the atmospheric condensation unit. The peptides in samples mainly resulted from the degradation of collagens and cytoskeleton proteins such as tubulin, actinin, and so on. The relative abundance of degraded collagens increased with the decrease of no-collagen proteins.
CONCLUSIONCollagen degradation mainly occurred during the atmospheric condensation unit, which was the key process affecting the composition of E-jiao.
Chromatography, Gel ; Chromatography, High Pressure Liquid ; methods ; Collagen ; analysis ; metabolism ; Mass Spectrometry ; methods ; Molecular Weight ; Peptides ; analysis
Result Analysis
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