Objective To explore the expression and significance of signal transducer and activator of transcription 3 (Stat 3), glucose transporter protein 1 (GluT-1) and proliferation cell nuclear antigen (PC NA) in lesions of condylomata acuminata (CA). Methods SP immunohistochemistry method was used to measure the expression of Stat 3, GluT-1 and PCNA in tissue samples from 40 cases of CA and 20 normal skin controls. Results The positivity rates of Stat 3, GluT-1 and PCNA were 85.0% (34/40), 87.5% (35/40) and 85.0%(34/40), respectively in CA tissue, 35.0% (7/20), 30.0% (6/20)and 55.0% (11/20),respectively in the control tissue; statistical difference was observed in these rates between the two groups (all P ＜ 0.05). The expression intensity of Stat 3, GluT-1 and PCNA was also higher in CA tissue than that in the controls. In addition, the expression intensity of PCNA was correlated with that of Stat 3 and GluT-1in CA tissue (both P＜ 0.05). Conclusions There is an overexpression of Star 3, GluT-1 and PCNA in CA tissue, and the overexpression of Stat 3 and GluT-1 may be associated with the over-proliferation of CA tissue.

A 10-day-old male infant presented with skin erythema and blisters for 6 days. Skin examination showed scattered or confluent erythema all over the body, tense blisters of varying sizes on the normal skin or an erythematous base, and some blisters were ulcerated and erosive; bloody bullae and erythematous erosive patches could be seen on the oral mucosa. Histopathological examination revealed subepidermal blisters, and there were some neutrophils and a few eosinophils in the blisters. Direct immunofluorescence assay showed homogeneous linear IgA and granular C3 deposits along the basement membrane zone, without IgG deposits. The diagnosis of neonatal linear IgA bullous dermatosis was confirmed. After comprehensive treatments including nutritional support and anti-infection treatment, skin erythema and blisters subsided, and the mucosal damage was attenuated. The telephone follow-up 16 months after discharge showed that the infant was in good general condition with normal growth and development, and the oral mucosal lesions had subsided and healed, without new skin lesions.

Objective To calibrate contact parameters of liver tissue discrete element model. Methods Based on MATLAB image processing technology, the accumulation angle of liver tissues was measured. The ‘Hertz-Mindlin with JKR’ contact model was used to simulate the accumulation angle of liver tissues. The orthogonal experiment was designed with the coefficient of rolling friction and the energy of JKR surface as factors. The parameters of the contact model were calibrated by batch processing, and the optimal parameter combination was verified by secondary simulation calibration. Results The accumulation angle obtained by the physical test was 11.2°±0.86°. In the orthogonal experiment, the accumulation angle of the 6th set of parameter combinations was 11.8°, and the relative error was 5.1%. The simulation test and the physical test had a high similarity in accumulation angle and shape. The sequence of factors affecting the accumulation angle was the JKR surface energy between the tissue particles and the stainless steel plate > the rolling friction coefficient between the tissue particles and the stainless steel plate > the JKR surface energy between the tissue particles and the tissue particles=the rolling friction coefficient between the tissue particles and the tissue particles. Conclusions The optimization parameter could be used to further conduct the discrete element simulation between the tissue particles and the pulverizer, so as to reveal the accumulation and flow state of the tissue particles under the action of myoma pulverizer.

With the development of minimally invasive surgery, many open surgery has been replaced by intracavity surgery. In laparoscopic surgery, an electric fibroid morcellator must be used to remove large tissue specimens from a small abdominal incision. Of course, there are some complications in the use, in order to follow the principle of no tumor, the doctor used the laparoscopic pouch in clinical operation to reduce the risk of spreading potential tumor tissue. There are various kinds of pouches, which are classified according to their existing state before use, it can be classified into two categories:overlapping and non-overlapping. The advantages and disadvantages of different bags and pockets are also analyzed. It provides a theoretical basis for technological innovation and equipment improvement.
Laparoscopy ; instrumentation ; Minimally Invasive Surgical Procedures