To improve the quality of medical postgraduate students in our school and to meet the needs of basic medicine and the development of personalized medicine , we opened the course“molecular pathology” to medical graduates. To make the molecular pathology the true bridge course between basic medicine and clinical medicine, we optimized the content of molecular pathology, cre-ated diagnostic technical platform matched with molecular pathology, rationalized management system worked in the basic pathology and clinical pathology. Practice has proved that “molecular pathology”curriculum promotes medical postgraduate students' transformation of medical philosophy, cultivates their innovation ability in scientific research and clinical practice ability etc.

Aim The purpose of this study was to compare the effect of AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE on hypoxic and normoxic rabbit pulmonary arterial rings and to discuss their roles in the formation of PA hypertension.Methods Twelve neonatal rabbits were randomly divided into two groups(n=6):the normoxic group breathing fresh gas(FiO_2 =21%) and the hypoxic group breathing hypoxic gas(FiO_2=10%).After 9 days,they were anesthetized with pentobarbital sodium,and the chest was opened for removal of the heart and lungs en bloc,then PA rings(0.5~1.5 mm in diameter and 3 mm length) were prepared.We used tension studies of PA rings to observe the effect of AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE on hypoxic and normoxic rabbit pulmonary arterial rings.Results ① AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE constricted normoxic rabbit PA rings in a dose-dependent manner,respectively.Constriction of normoxic rabbit PA rings induced by 15-KETE、8(S),15(S)-DiHETE was significant,but that of AA、15-HETE wasn′t significant.② AA、15-HETE、15-KETE and 8(S),15(S)-DiHETE increased hypoxic rabbit PA rings' tension.Constriction of AA、15-HETE on rabbit PA rings in hypoxic group was significantly greater than that of normoxic group(P0.05).③ Constriction of 15-KETE on rabbit PA rings in normoxic group was significantly greater than that of hypoxia group(P

Aim To investigate the effect of 15-ketoeicosatetraenoic acid(15-KETE)and its mechanism through ion channels on rat isolated pulmonary arterial rings by using organ bath technique.Methods Sixteen healthy Wistar rats weighing 220?20 g were divided into two groups(n=8): normoxia group breathing fresh air(FiO_2=21%) and hypoxia group breathing hypoxic air(FiO_2=10%) in a hypoxic box.Pulmonary arteries(PA)were extracted after 9 d and cut into rings(0.5~1.0 mm in diameter and 3 mm in length) for organ bath experiments.Results(1) With increasing concentration from 0 to10~(-6) mol?L~(-1),15-KETE increased PA rings tension gradually in a dosedependent fashion;(2) 4-aminopyridine(2 mmol?L~(-1)),a Kv channel blocker significantly decreased constriction of rat isolated PA rings induced by 15-KETE,and results were similar in two groups;(3) The K_(ATP) channel blocker glyburide(10~(-6)6 mol?L~(-1)) and the BK_(Ca) channel blocker tetraethylammonium(10 mmol?L~(-1)) did not affect constriction of rat isolated PA rings induced by 15-KETE;(4) The BKCa channel blocker nifedipine(10~(-6) mol?L~(-1)) and Ca~(2+)-free Krebs solution significantly decreased constriction of rat isolated PA rings induced by 15-KETE.Conclusion Kv channels play a role in constriction of PA induced by 15-KETE;L-type Ca~(2+) channel blocker and extracellular calcium ion also influence constriction of isolated PA rings induced by 15-KETE.

OBJECTIVETo measure and compare the content of d-borneol in the different parts of Cinnamomum camphora by GC-MS.

METHODWith water-steam distillation and GC-MS method, d-borneol was extracted and determined.

RESULTThe linear range of d-borneol was 0.4-2.8 microg (r = 0.999 9). The average recovery was 95.40%, and RSD was 0.56%.

CONCLUSIONThe method is simple and accurate with good separation. The content of d-borneol in the dried leaves 63.97% in the crude exfract. It can provide the proof of the exploitation of C. camphora.

Bornanes ; analysis ; Cinnamomum camphora ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods ; Plant Extracts ; analysis ; Plant Structures ; chemistry

This study was intended to observe the biological properties of primary human embryonic skeletal myoblasts cultured in vitro and to inquire about the possible differentiation mechanism. Isolated human embryonic myoblasts were identified by morphology and myosin immunohischemical staining. The proliferating myoblasts were shifted to DM (DMEM supplemented with 3% fetal bovine serum) to induce differentiation. The control group was cultured in GM (DMEM supplemented with 10% fetal bovine serum). The differentiation was tested by the rate of myotube formation (RMF); The change of cell cycle was tested by flow cytometry; the morphological features were observed by use of inverted microscope and TEM. The myogenic regulatory factors (MRFs) such as MyoD, Myogenin and Myf5 were assayed by RT-PCR. Results showed that the primary myoblasts cultured in DM, in comparison with those cultured in GM, had higher rate of myotube formation (RMF) and myofilament formation, and more of the myoblasts cultured in DM exited the S phase. The expression level of Myogenin mRNA was obviously higher than that of the control group, the increase of MyoD mRNA expression level was not so high, however, the expression of Myf5 mRNA was decreased. These data indicate that there is a possible mechanism between differentiation and cell cycle. Besides, the mRNA expression of myogenic regulatory factors (MRFs) occurs in different phase of differentiating myoblastes and implicates the diverse biological function.
Cell Cycle ; Cell Differentiation ; Cell Division ; Cells, Cultured ; Embryo, Mammalian ; Gene Expression Regulation, Developmental ; Humans ; Muscle Fibers, Skeletal ; cytology ; Myoblasts ; cytology ; metabolism ; ultrastructure ; Myogenin ; biosynthesis ; genetics ; RNA, Messenger ; biosynthesis ; genetics