Objective To amend the errors between projective image center and the actual rotational center and improve the quality of reconstructed images. Methods The impact of reconstructed image in which the projection center and the actual center caused the deviation is analyzed, and provides the ways that is based on the gray value difference of objects and background in projection image to calculate actual rotational center, and then mends the projective image based on the actual rotational center. Results The difference between the actual rotational center and the calculated projective image center is less than one pixel. The quality of the reconstructed images is improved greatly. Conclusion The correction method is based on projective images, no need to increase the auxiliary conditions and the number of projection images. And the way as the pre-process of reconstruction, only needs small amount calculation, doesn't affect the reconstructive speed.

Objective To summarize the experience in diagnosis and treatment for solid-pseudopapillary tumors of the pancreas (SPT). Methods A retrospective clinical analysis about clinical, imaging and pathologic data was made on 16 cases of SPT admitted from January 2005 to December 2009. Results Five had SPT in the head of the pancreas, 5 in the body of the pancreas, 6 in the tail of the pancreas. The first symptom was intermittent epigastric pain ( n = 7), abdominal aponia mass ( n = 3), Pancreatic tumor found by chance (n =4), weight loss (n =2). Solid and Solid-cystic masses of low echo were found in US. Masses of low density in pancreas were found on CT scan, while irregular enhancement appeared in the circumference of all tumors in enhanced CT scan sequences. Tumor markers in patients' erum were all negative.9 patients underwent distal pancreatectomy and spleen resection, including 1 patient also underwent left hemicolectomy. Local excision of tumor was performed in 4 cases. Pancreatic local excision and pancreaticojejunostomy were performed in 3 cases. 14 cases were followed up with an period of from 3 to 48 months. No evidence of relapses and metastasis in these cases was found. Conclusion SPT primarily affects young women, and it may be located in any part of pancreas. Surgical resection is recommended as the treatment of choice. The prognosis is good.

BACKGROUND: In recent years, epidemiological studies have found that the saturation of transferring or the increased level of serum ferritin are associated with the attacks of cancer, coronary heart disease, diabetes mellitus, Parkinson disease, liver disease and the diseases of immune system. Therefore, it is suggested that the intake of excessive iron may cause adverse influence on the healthy of human body.OBJECTIVE: To establish high-iron model in mice by using full-rate diet pellets by adding regular quantitative intraperitoneal injection of iron dextran, and observe the iron levels in vivo and the changes of organ coefficients.DESIGN: A comparative observation.SETTING: Department of Nutrition and Food Hygiene, School of Public Health and Tropic Medicine, Southern Medical University.MATERIALS: The experiment was carried out in the laboratory of the Department of Nutrition and Food Hygiene, School of Public Health and Tropic Medicine, Southern Medical University in May 2006. Forty Kunming mice of SPF grade, 20 males and 20 females, weighing 18-22 g, were provided by the Animal Experimental Center of Southern Medical University. The mice were randomly divided into control group (n =10) and high-iron model group (n =30) by introperitoneal injection of saline and iron dextran respectively, and the latter group was subdivided into low, middle and high-dosage groups (6.25, 12.5 and 25 g/L) respectively, 10 mice in each group. Full-rate diet pellets (iron content was 370 mg/kg)were purchased from the Animal Experimental Center of Southern Medical University. Iron dextran reagent (norm: 2 mL containing 50 mg iron) was the product of Zhejiang Ruian Pharmaceutical Factory (certification number: H33021758).The kits of superoxide dismutase (SOD) and maldondialdehyde (MDA) were provided by the Nanjing Jiancheng Bioengineering Institute.METHODS: Mice in each group were raised in plastic stainless steel cages respectively at (23±3) ℃, and they were free to the access of food and deionized water. Mice in the low, middle and high-dosage group were treated with intraperitoneal injection of iron dextran once every other day, 0.8 mL for each time, whereas iron dextran was replaced by saline in the control group, all the mice were treated for 6 weeks, and their nutritionol conditions were observed. All the mice were killed at the end of the 6th week. The iron contents in organs of heart, liver, spleen, lung and kidney, and serum were determined with atomic absorption spectrophotometer and automatic biochemical analyzer respectively; Pathohistological examination of organs were performed; The organ coefficients of liver and spleen were calculated; MDA content and SOD activity in serum were determined.MAIN OUTCOME MEASURES: General conditions of mice in each group; Iron contents in organs and iron concentration in serum; Organ coefficients of liver and spleen; MDA content and SOD activity in serum; Pathological changes.RESULTS: In the high-iron model group, the body figures of the mice were changed, body masses were obviously decreased. The iron contents in organs and serum of mice in the high-iron model group were all obviously increased as compared with those in the control group (t =5.841, P ＜ 0.01), the organ coefficients of liver and spleen were also markedly increased (t =5.841, P ＜ 0.01), which were all in a dosage-dependent manner. The MDA content in serum was obviously increased (t =5.841, P ＜ 0.01) whereas the SOD activity was obviously decreased (t =12.924, P ＜ 0.01) as compared with those in the control group. The pathohistological examination under light microscope showed that there were pathological damages of different degree occurred in the tissue and cells and cell degeneration was observed,which affected the normal physiological function of cells.CONCLUSION: High-iron mice models can be successfully established by the intraperitoneal injection of iron dextran.The storage of excessive iron in vivo will result in the organic damages.

BACKGROUND: Iron deficiency anemia (IDA) is one of the highest incidence nutritional-deficiency diseases all over the world; especially infants and children are the main group. IDA presently becomes one of the most important nutritional problems to be solved.OBJECTIVE: To observe the effect of chocolate carrier and orange juice on recovery of IDA model rats.DESIGN: Randomized controlled animal study.SETTING: Laboratory of Nutrient and Food Hygiene, School of Public Health and Tropical Medicine, Southern Medical University.MATERIALS: The experiment was carried out in the Laboratory of Nutri ent and Food Hygiene, School of Public Health And Tropiacal Medicine, Southern Medical University from March to June 2006. A total of 60 healthy SD rats of clean grade were provided by Animal Center of Southern Medical University (certification: 2002-009 2005A047). METHODS: ① Establishment of IDA models: Among them, 20 rats of half genders were randomly selected toregard as control group, and other 40 were regarded as model group. Rats in control group were fed with rou tinefeed and drank freely. Rats in model group were fed with AOAC-modi fied low-dosage iron feeds to establish IDA models by blooding at caudal vein. Three weeks later, average concentration of ferrohemoglobin in model group was decreased to about 90 g/L, and this suggested that model estab lishment was successful. Ten rats of half genders in each group were ran domly sacrificed. Pre-experiment and 3 weeks of post-experiment, rats were weighed to measure concentration of ferrohemoglobin with hemoglobin cyanide (HiCN) technique, red blood cell count (RBC, direct method), serum iron (microparticle chemiluminescent immunoassa y and related kit) and concentration of serum transferrin receptor (STFR, ELISA method and related kit). ② Recovery test: Other 10 rats in control group were regarded as normal control group, and they were fed with routine feed and drank freely. The rest 30 rats of half genders in model group were randomly di vided into 3 subgroups: model control group, FeSO4 group and chocolate & orange juice group with 10 in each group. Rats in model control group were perfused with distilled water everyday; rats in FeSO4 group were per fused with FeSO4, and rats in chocolate & orange juice group were per fused with chocolate carrier and orange juice. The iron volume in the last two groups was 6 mg/(kg·d). At 40 days after intervention, the experiment was stopped. Concentration of ferrohemoglobin, RBC, serum iron, concentration of STFR and activity of plasma-protein aconitase were measured with atom-trapping atomic-absorption spectrophotometry; meanwhile, biological utilization rate of chocolate carrier & orange juice was calculated.MAIN OUTCOME MEASURES: ① Contents of herrohemoglobin, RBC,serum iron and STFR before experiment and after modeling; ② contents of ferrohemoglobin, RBC, serum iron, STFR and activity of plasma-protein aconitase before recovery test and at 40 days after experiment; ③ Related biological utilization rate.RESULTS: All 60 rats were involved in the final analysis without any loss. ① Comparison of blood index after modelling: Content of ferrohemoglobin, RBC and content of serum iron were lower in model group than those in control group (P ＜ 0.01), but content of STFR was higher than that in control group (P ＜ 0.01). ② Comparison of blood index and activity of plasma-protein aconitase in liver before recovery test and at 40 days after experiment: At 40 days after intervention, concentration of ferrohemoglobin,RBC and content of serum iron were higher in FeSO4 group and chocolate & orange juice group than those in model control group (P＜ 0.01); however, content of STFR was lower than that in model control group (P ＜ 0.01).At 40 days after intervention, activity of plasma-protein aconitase in FeSO4 group and chocolate & orange juice group was higher than those before recovery test (P ＜ 0.01). ③ Related biological utilization rate of chocolate carrier plus orange juice: Biological utilization rate of FeSO4 was regarded as 100%, and biological utilization rate of chocolate carrier plus orange juice was increased remarkably (106.7%).CONCLUSION: Chocolate carrier plus orange juice can improve IDA function and wildly use on treating IDA because of its good absorption. It is characterized by well biological utilization rate and good taste; therefore,it is a hot topic for trophology and foods produce presently.

Objective To discuss some expectation on reform of classroom teaching method for eight-year program medical education, in order to provide a basis for the evaluation and reform of classroom teaching method. Methods Investigation that mainly included expectation on reform of classroom teaching method, classroom teaching method of most expected application, and ex-pected value of classroom teaching method was investigated and analyzed with self-made question-naire, to total 78 medical students (22 of Grade 2008, 20 of Grade 2009, 20 of Grade 2010, 16 of Grade 2011) of eight-year program who had completed the stage before medical education in the uni-versity, and related 32 teachers. Software of SPSS 20.0 was used to make statistical analysis. Results In the survey, 55.8% students and 78.1% teachers hoped to reform the current classroom teaching methods (χ2=4.782,P=0.029). 61%students and 90.6% teachers expected comprehensive application of diverse method(χ2=15.464,P=0.017), including instruction combined with writing on blackboard, teaching by multimedia, PBL, inversion-type class, independent learning, a lot of questions for exami-nation, and so on. The value expectation (>60%) of classroom teaching method was multivariate, in-cluding to stimulate students' interest in learning, to cultivate ability of finding and solving problems, conducive to understanding of knowledge and memory, conducive to comprehension and memory of knowledge, conducive to cultivation of learning method, able to improve ability of creativity and criti-cal thinking, conducive to strengthening interaction and communication of teachers and students, to improve the subject consciousness of student in classroom, helpful to cultivate the spirit of teamwork, to improve the efficiency of teaching, and able to make full use of information technology. Conclusions For the diversified value demand and the highly expectation on reform of current classroom teaching method, more attention should be paid to evaluation and guidance on reform of classroom teaching method for eight-year program medical education.

Objective To investigate the establishment and effect of the oncological training base for professional nurses in Xinjiang Uygur Autonomous Region.Methods The oncological training base for professional nurses in Xinjiang was established to train 179 nursing specialists.Result One hundred and seventy-nine participants passed the qualification examinations,achieving an average score of (77.1±5.8)in the theoretical examination and(78.0±8.9)in the skills examination.Conclusions The establishment of oncological training base for professional nurses is effective in improving the professional knowledge and operational levels of professional nurses on oncology and promoting the development of oncological nursing.

Objective:To investigate the effect on the expression of Slug for the trasfection of miR-200c combined with the research on the ability of migration of breast cancer cell BT549.Methods:Chemically synthesized miR-200c mimic was trasfected into BT549 cells,which have high metastatic potential.The effect on the ability of migration of breast cancer cell BT549 for the transfection of miR-200c was analyzed by Transwell migration assay and Wound healing assay.The expression of Slug and E-cadherin mRNA was detected by Real-time PCR.The expression of Slug protein was detected by Western blot.Results:Transfection with miR-200c mimic significantly down-regulated the expression of Slug as compared with the control group (P<0.05).BT549 cell tranfected with miR-200c mimic had lower levels of migration capacity than cells in the control group (P<0.05).Conclusion:miR-200c inhibits Epithelial-mes-enchymal transition by suppressing Slug leading to down-regulation of migration capacity of breast cancer cell BT549.

Objective To find out related proteins that could possibly predict curative effects of neoadjuvant chemotherapy(NACT) by applying surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF MS)to detect the difference in protein spectrum of breast cancer tissues.Methods Cancer tissue samples from 30 cases of breast cancer patients were detected by SELDI-TOF MS before NACT to get protein spectrum of breast cancer tissues.After NACT,the curative effects were evaluated according to RECIST standard and patients were divided into effective group(19 cases with complete response or partial response)and invalid group (11 cases with stable disease or progressive disease).Results 11 different protein peaks (P ＜ 0.05) were screened out:3491 Da(t =3.189 P =0.004),5158 Da(t =3.897 P =0.001),5360 Da(t =2.157 P =0.04),7549 Da(t =2.173 P=0.038),8451 Da(t =2.258 P =0.032),8694 Da(t =3.234 P =0.003),9089 Da (t =2.653 P =0.013),10528 Da(t =3.127 P =0.004),13445 Da(t =2.231 P =0.034),15118 Da(t =3.255 P =0.003),and 44065 Da(t =2.554 P =0.017).They were all expressed higher in effective group than in invalid group.The difference had statistical significance(P ＜ 0.05).Conclusion SELDI-TOF MS technology can screen out protein spectrum sensitive to NACT,providing evidences for breast cancer patients whether or not taking NACT.

Objective To assess roles of vascular endothelial growth factor(VEGF)and plateletderived growth factor(PDGF)in the mechanisms of lymphangiogenesis in epithelial ovarian carcinoma.Methods (1)Expression of Proxl,a newly described lymphatic endothelial cell nucleus marker,VEGF-A,VEGF-C,VEGF-D and PDGF-A,PDGF-B,PDGF-C,PDGF-D were detected bv RT-PCR in SKOV3 cell line and in 90 ovarian tissue samples,included 15 benigh tumors 10 borderline tumors, 45 malignant tumors and 20 normal ovarian samples.(2)Expression levels of Proxl,VEGF-A,-C,-D and PDGF-A,-B,-C,-D were detected in 90 ovarian tissue sample mentioned above by real-time quantitative PCR(RTQPCR).Resuls (1)Proxl was expressed in ovarian samples mentioned above,while not detected in SKOV3 cell. VEGF-A,-C,-D and PDGF-A,-B,-C,-D were found in SKOV3 cell and various ovarian tissues.(2)Expression levels of Proxl(2.2±1.3,P＜0.01),VEGF-A(3.5±1.5,P＜0.01),VEGF-C(19±14.P＜0.01),VEGF-D(3.0±1.8,P＜0.01)and PDGF-A(3.3±3.3,P＜0.05),PDGF-C(6.9±4.6,P＜0.01)in malignant group were found to be significantly higher than those in borderline group and benign group.(3)The expression levels of Proxl,VEGF-A and PDGF-A were significantly greater in samples from the patients with lymph node metastasis(Proxl:3.0±1.4,VEGF-A:4.1± 1.7,PDGF-A:4.9±4.1),peritoneum metastasis(Proxl:2.8±0.9,VEGF-A:4.0±1. 8,PDGF-A:4.5±4.0)and in stage Ⅲ-Ⅳ(Proxl:2.6±1.3,VEGF-A:4.0±1.4,PDGF-A:4.1±3.7)than those without lymph node metastasis,without peritoneum metastasis and in stage Ⅰ-Ⅱ.There was a significant increased in the degree of VEGF-C and VEGF-D expression in positive lymph node metastasis group(VEGF-C:24±13,VEGF-D:3.9±2.0)compared with negative group(P＜0.05).(4)There were significant positive correlations between the expression levels of Proxl and VEGF-D(r=0.62,P＜0.01),PDGF-C(r=0.91,P＜0.01)or PDGF-D(r=0.61.P＜0.01).Conclusions VEGF-A,VEGF-C and PDGF-A may promote lymphatic metastasis in epithelial ovarian carcinoma though else mechanisms other than lymphangiogenesis.VEGF-D may facilitate lymphangiogenesis and lymph node metastasis in epithelial ovarian cancer.There is no significant correlation between the expression of PDGF-B and lymphangiogenesis and lymph node metastasis.PCGF-C and PDGF-D may motivate lymphangiogenesis,but could not participate in lymph node metastasis in ovarian carcinoma.

Objective To explore the relationship between the hepatic injury and the severity of the patients with non-gallstone severe acute pancreatitis,so as to provide some evidences in the treatment on the hepatic injury,to lower the systemic and local applications of SAP,as well as lower the morality of SAP.Methods Patients suffering from non-gallstone SAP were retrospectively analyzed.We statisticated the occurrence of multiple organ dysfunction syndrome.We adopted the occurrence of MODS of the patients as the indicatrix of prognosis and analyzed the results.Univariate analysis and multivariate logistic regression analysis were used to analyze the ralationship between the markers of heapatic injury and the prognosis of the patients suffered of non-gallstone SAP.Results The univariate logistic regression analysis of the relationship between hepatic dysfunction and the occur of MODS of non-gallstone SAP shows that there were correlation between the occur of MODS and AST,ALT,ALB,respectively.The multivariate logistic regression analysis shows that there were correlation between the occur of MODS and AST,ALT,ALB,respectively.Conclusion The study showed that the heighten of the serum consentration of AST,ALT,TB and the degrade of the serum consentration of Alb were the independent influencing factors of patients with non-gallstone SAP,respectively,and suggested the unfavourable prognosis of the patients.