Objective To prepare the HPV genotyping control materials. Methods Three hundred cervical smears with different HPV genotypes were collected and detected by surface plasmon resonance and sequencing. The primers for specific genotype were designed according to GenBank. The recombinant plasmid was constructed through PCR amplification, ligation and transformation. Thirty recombinant plasmids were identified through PCR amplification, sequencing, and the sequences were compared using BLAST. Results The collected HPV infectious samples contained 30 different genotypes including HPV 6,16,18 and so on.The fragment sequences of PCR amplification were concordant with the designed. The fragment sizes of the other types ranged from 1 500 to 2 000 bp except HPV CP8304. And the 30 recombinant plasmids identified by PCR were concordant with the target. The identity of BLAST was 99%. In the fragment of 1500 bp in length, 11 bases were inconsistent with the reference sequence. Conclusions Genotyping control materials were developed successfully. The human papillomavirns genotyping control materials covered all the most common types and included 14 types with high-risk, 3 types with medium-risk and 13 types with low-risk.

BACKGROUND:In vitro lymphocyte proliferation test is often used to detect the potential immunogenicity of medical devices,but no detailed extraction conditions and dose are given in the relevant standards. OBJECTIVE:To investigate the effects of different extraction conditions of the test product and different doses of the extract on in vitro human lymphocyte proliferation,and to consider the factors that need to be considered when selecting test conditions for in vitro lymphocyte proliferation test. METHODS:In the experiment,the homogenous bone repair material and heparin-modified intraocular lens were divided into the following 12 groups:(1)Experimental group 1:24-hour complete medium(RPMI modified medium containing 10%fetal bovine serum)extract of 200 μL + lymphocyte suspension of 50 μL;(2)negative control group 1:24-hour complete medium 200 μL + lymphocyte suspension 50 μL;(3)experimental group 2:24-hour complete medium extract 100 μL + lymphocyte suspension 100 μL;(4)negative control group 2:24-hour complete medium 100 μL + lymphocyte suspension 100 μL;(5)experimental group 3:72-hour RPMI modified medium extract(addition of 10%fetal bovine serum before experiment)200 μL + lymphocyte suspension 50 μL;(6)negative control group 3:72-hour RPMI modified medium(addition of 10%fetal bovine serum before experiment)200 μL + lymphocyte suspension 50 μL;(7)experimental group 4:72-hour RPMI modified medium extract(addition of 10%fetal bovine serum before experiment)100 μL + lymphocyte suspension 100 μL;(8)negative control group 4:72-hour RPMI modified medium(addition of 10%fetal bovine serum before experiment)100 μL + lymphocyte suspension 100 μL;(9)positive control group 1:complete medium containing 10 μg/mL plant hemagglutinin-M 200 μL + lymphocyte suspension 50 μL;(10)positive control group 2:complete medium containing 10 μg/mL plant hemagglutinin-M 100 μL + lymphocyte suspension 100 μL;(11)blank control group 1:250 μL complete medium;(12)control group 2:200 μL complete medium.After 3 days of culture,the proliferation of lymphocytes was detected by CCK-8 assay. RESULTS AND CONCLUSION:(1)Under different test conditions,the extracts of the allogeneic bone repair material could enhance the activity of human lymphocytes.Under the condition of 72-hour leaching in RPMI modified medium and the volume ratio of leaching solution and lymphocyte suspension was 4:1,the most significant effect was observed.Heparin-modified intraocular lens extract also had obvious inhibitory effect on lymphocyte activity under this condition;its inhibitory effect on lymphocyte activity may be related to the heparin in the extract.However,the activity of lymphocytes was slightly enhanced by heparin-modified intraocular lens extract under the experimental conditions of complete medium extraction for 24 hours and the volume ratio of extract to lymphocyte suspension was 4:1.(2)Under different extraction conditions and doses,the results of in vitro lymphocyte proliferation test may be quite different.The selection of test conditions should be combined with the clinical application of the product,and the inherent characteristics of the product should also be considered.

Objective To explore the effect of the full-time rehabilitation nursing on the functional recovery after the noncemented total hip replacement .Methods One hundred and fourteen patients with noncemented total hip replacement were chosen and randomly divided into the control group ( n=51 ) and the observation group (n=63).The control group received the random rehabilitation nursing through the executive nurses, and the observation group received the full-time rehabilitation nursing through the full-time rehabilitation nurses.Results The scores of Harris at 1 d before discharge , 6 weeks after the operation and 3 months after the surgery were respectively (64.9 ±6.4), (78.5 ±5.1), (90.0 ±4.2) in the observation group, and were higher than those in the control group , and the differences were statistically significant ( t =5.377,3.059, 4.288, respectively;P<0.01).The scores of pain degree , living ability, walk ability at 1 d before discharge, 6 weeks after the operation and 3 moths after the operation between two groups had statistical significances ( P<0.01);when two groups of patients discharged hip X-ray showed good prosthesis , no exception.Experimental group patients with complications of 0.0%, the control group in the incidence of complications was 11.8%, the difference was statistically significant (χ2 =6.981,P <0.01).Conclusions The full-time rehabilitation nursing promotes the functional recovery after the noncemented total hip replacement .

Objective:To retrospectively analyze the effectiveness of Caprini thrombosis risk assessment model in assessing the risk of deep venous thrombosis (DVT) in orthopedic patients.Methods:Using the convenient sampling method, 52 orthopedic patients who were diagnosed with DVT in Anhui Provincial Hospital from April 2016 to March 2019 were selected as the DVT group. According to the ratio of 1 to 2, 104 patients with non-DVT in Department of Orthopedics were selected as the non-DVT group. The Caprini risk assessment model was used to carry out DVT risk scoring and risk classification, and the relationship between different risk grading and occurrence risk of DVT in patients was explored. Logistic regression was used to explore the risk factors of DVT in orthopedic patients.Results:The Caprini score of the DVT group was (12.25±4.26) , which was higher than (7.18±2.92) of the non-DVT group, and the difference was statistically significant ( t=7.727, P<0.01) . The difference between the DVT group and the non-DVT group in Caprini risk classification was statistically significant (χ 2=8.778, P<0.05) . In the Caprini risk classification, the risk of DVT in very high-risk patients was 7.099 (95% CI: 1.604-31.412) times higher than that in non-very high-risk patients. Logistic regression analysis showed that BMI≥25 kg/m 2, bed rest time≥72 h, lower extremity swelling, plaster/limb fixation, and selective lower extremity joint replacement surgery were 5 high-risk factors for patients with DVT ( P<0.05) . Conclusions:The Caprini risk assessment model has a good predict ability for the risk of DVT in orthopedic patients. Nursing staff can carry out targeted nursing intervention according to the high-risk factors of DVT. Stratified management of this model can also enable effective hierarchical prevention of DVT risk groups and ensure the safety of patients to the maximum extent, which is worthy of clinical application.

[Abstract] [Objective] To investigate the clinical efficacy of intrauterine perfusion of different concentrations of platelet-rich plasma (PRP) in the treatment of thin endometrium. [Methods] A total of 35 cases of infertility patients who underwent autologous PRP intrauterine perfusion to improve thin endometrium from January 2022 to February 2024 at Run Run Shaw Hospital affiliated to Zhejiang University School of Medicine were selected as the study objects. The significance of intrauterine perfusion of PRP on endometrium thickening was evaluated by analyzing the difference in endometrial thickness before and after ovulation with different concentrations of PRP perfusion. The effect of PRP on thin endometrial infertility was evaluated by pregnancy outcome index. The influence of endometrial thickness on pregnancy outcome was evaluated by the difference of endometrial thickness between pregnant group and non-pregnant group after autologous PRP perfusion, and the correlation of each index was analyzed. [Results] Among 35 cases of infertility, the endometrial thickness was (4.77±0.87) mm before intrauterine PRP perfusion and (6.13±0.85) mm after intrauterine PRP perfusion, with statistically significant difference (P<0.05). The increased endometrial thickness was (1.03±0.31) mm, (1.31±0.47) mm and (1.70±0.85) mm in patients with low, medium and high concentration autologous PRP intrauterine perfusion respectively. The pregnancy outcome of 35 patients showed that a clinical pregnancy rate of 31.4% (11/35) and an early miscarriage rate of 0. Statistical analysis of the endometrial thickness during ovulation after PRP perfusion revealed that the endometrial thickness in the pregnant group was (6.64±0.91) mm while in the non-pregnant group it was (5.86±0.69) mm, with statistically significant difference (P<0.05). [Conclusion] Intrauterine perfusion of autologous PRP in patients with thin endometrial infertility is helpful for endometrial thickening, and PRP with high concentration of platelets is more effective, thus better improving pregnancy outcome, and has clinical significance for the treatment of thin endometrial infertility.