A novel unsaturated polyphosphoester (UPPE) was devised in our previous research, which is a kind of promising scaffold for improving bone regeneration. However, the polymerization process of UPPE scaffolds was unfavorable, which may adversely affect the bioactivity of osteoinductive molecules added if necessary, such as recombinant human bone morphogenetic protein-2 (rhBMP2). The purpose of this study was to build a kind of optimal scaffold named UPPE-PLGA-rhBMP2 (UPB) and to investigate the bioactivity of rhBMP2 in this scaffold. Furthermore, the cytotoxicity and biocompatibility of UPB scaffold was assessed in vitro. A W1/O/W2 method was used to fabricate PLGA-rhBMP2 microspheres, and then the microspheres were added to UPPE for synthesizing UPB scaffold. The morphological characters of PLGA-rhBMP2 microspheres and UPB scaffolds were observed under the scanning electron microscopy and laser scanning confocal microscopy. The cumulative release of UPB scaffolds was detected by using ELISA. The cytotoxicity and biocompatibility of UPB scaffolds were evaluated through examining the adsorption and apoptosis of bone marrow stromal cells (bMSCs) seeded on the surface of UPB scaffolds. The bioactivity of rhBMP2 in UPB scaffolds was assessed through measuring the alkaline phosphates (ALP) activity in bMSCs seeded. The results showed that UPB scaffolds sequentially exhibited burst and sustained release of rhBMP2. The cytotoxicity was greatly reduced when the scaffolds were immersed in buffer solution for 2 h. bMSCs attached and grew on the surface of soaked UPB scaffolds, exerting well biocompatibility. The ALP activity of bMSCs seeded was significantly enhanced, indicating that the bioactivity of rhBMP2 remained and still took effect after the unfavorable polymerization process of scaffolds. It was concluded that UPB scaffolds have low cytotoxicity, good biocompatibility and preserve bioactivity of rhBMP2. UPB scaffolds are promising in improving bone regeneration.

A novel unsaturated polyphosphoester (UPPE) was devised in our previous research, which is a kind of promising scaffold for improving bone regeneration. However, the polymerization process of UPPE scaffolds was unfavorable, which may adversely affect the bioactivity of osteoinductive molecules added if necessary, such as recombinant human bone morphogenetic protein-2 (rhBMP2). The purpose of this study was to build a kind of optimal scaffold named UPPE-PLGA-rhBMP2 (UPB) and to investigate the bioactivity of rhBMP2 in this scaffold. Furthermore, the cytotoxicity and biocompatibility of UPB scaffold was assessed in vitro. A W1/O/W2 method was used to fabricate PLGA-rhBMP2 microspheres, and then the microspheres were added to UPPE for synthesizing UPB scaffold. The morphological characters of PLGA-rhBMP2 microspheres and UPB scaffolds were observed under the scanning electron microscopy and laser scanning confocal microscopy. The cumulative release of UPB scaffolds was detected by using ELISA. The cytotoxicity and biocompatibility of UPB scaffolds were evaluated through examining the adsorption and apoptosis of bone marrow stromal cells (bMSCs) seeded on the surface of UPB scaffolds. The bioactivity of rhBMP2 in UPB scaffolds was assessed through measuring the alkaline phosphates (ALP) activity in bMSCs seeded. The results showed that UPB scaffolds sequentially exhibited burst and sustained release of rhBMP2. The cytotoxicity was greatly reduced when the scaffolds were immersed in buffer solution for 2 h. bMSCs attached and grew on the surface of soaked UPB scaffolds, exerting well biocompatibility. The ALP activity of bMSCs seeded was significantly enhanced, indicating that the bioactivity of rhBMP2 remained and still took effect after the unfavorable polymerization process of scaffolds. It was concluded that UPB scaffolds have low cytotoxicity, good biocompatibility and preserve bioactivity of rhBMP2. UPB scaffolds are promising in improving bone regeneration.
Bone Morphogenetic Protein 2 ; chemistry ; pharmacology ; Bone Regeneration ; drug effects ; Humans ; Lactic Acid ; chemistry ; pharmacology ; Phosphatidylinositol Phosphates ; chemistry ; pharmacology ; Polyglycolic Acid ; chemistry ; pharmacology ; Tissue Scaffolds

Objective To investigate the symptom clusters of gastric cancer patients before chemotherapy,so as to provide a theoretical basis for the development of targeted management strategies.Methods A total of 228 gastric cancer patients who were treated in the General Surgery Department and Oncology Department of the Second Affiliated Hospital of Zhengzhou University from September 2016 to August 2017 were selected as the survey subjects by convenience sampling method.A cross-sectional survey was used to conduct a questionnaire survey.The Chinese Version Memorial Symptom Assessment Scale(MSAS)was used to evaluate the patients' symptoms before chemotherapy.Correspondence analysis was used to extract symptom clusters.Results A total of 228 questionnaires were distributed,and 224 were effective.The effective recovery rate was 98.2％.Before chemotherapy,the 5 most prevalent symptoms of gastric cancer patients were weight loss(97.8％),dry mouth(95.1％),pain(90.6％),sweating(84.4％)and lack of vitality/fatigue(79.5％).The top 5 symptoms of severity were lack of vitality/fatigue,difficult to focus,body swelling/bloating,swelling of the arms or legs and anxiety.Using the correspondence analysis to get 5 symptom clusters,the 5 symptom clusters were the energy deficiency symptom cluster,digestive tract symptom cluster,physical and mental symptom cluster,surgical related symptom cluster and neurological symptom cluster.The Cronbach's α coefficient was 0.95,0.89,0.92,0.93 and 0.87 respectively.Conclusions This study clarified the pre-chemotherapy symptom clusters of gastric cancer patients,and provided a scientific basis for exploring practical and effective methods of symptom management.At the same time,this research can also provide reference for the applicationof correspondence analysis method in the study of cancer symptom clusters.

Objective To compare clinical effects on treatment of cerebrospinal fluid leakage using various deep fascia suture techniques in lumbar posterior surgery.Methods There were 39 cases with cerebrospinal fluid leakage after lumbar posterior surgery in our hospital,in whom 22 cases were treated with deep fascia continuous suture,and 17 cases were treated with deep fascia interrupted suture.The extubation time,ambulation time,healing time and VAS scores in different times were recorded.Results The average extubation time,ambulation time,healing time of continuous suture group were better than that in interrupted suture group.The VAS scores on 1,3,7,14 d after operation in the continuous suture group was 3.55,2.64,1.18,0.36,and were 3.35,4.18,2.18,0.59 in the interrupted suture group.There were statistically significant differences in VAS scores on 3,7 d after operation between continuous suture group and interrupted suture group,while no significant differences were found in VAS scores on 1,14 d after operation.Conclusion Deep fascia continuous suture method has better effect on the treatment of cerebrospinal fluid leakage after lumbar posterior surgery.

Objective To compare clinical effects on treatment of cerebrospinal fluid leakage using various deep fascia suture techniques in lumbar posterior surgery.Methods There were 39 cases with cerebrospinal fluid leakage after lumbar posterior surgery in our hospital,in whom 22 cases were treated with deep fascia continuous suture,and 17 cases were treated with deep fascia interrupted suture.The extubation time,ambulation time,healing time and VAS scores in different times were recorded.Results The average extubation time,ambulation time,healing time of continuous suture group were better than that in interrupted suture group.The VAS scores on 1,3,7,14 d after operation in the continuous suture group was 3.55,2.64,1.18,0.36,and were 3.35,4.18,2.18,0.59 in the interrupted suture group.There were statistically significant differences in VAS scores on 3,7 d after operation between continuous suture group and interrupted suture group,while no significant differences were found in VAS scores on 1,14 d after operation.Conclusion Deep fascia continuous suture method has better effect on the treatment of cerebrospinal fluid leakage after lumbar posterior surgery.

Objective:To explore the current situation of evidence-based practice competence of nurses with master's degree at Class Ⅲ hospitals in Henan Province and its influencing factors so as to provide a reference for managers to formulate the targeted continuing education plan.Methods:From March 2019 to April 2019, we selected 133 nurses with master's degree of 6 ClassⅢ hospitals in Zhengzhou City of Henan Province as subjects by purposive sampling. All of nurses were investigated with the Chinese version of Evidence Based Practice Evaluation Competence Questionnaire (EBP-COQ) and the Barriers to Research Utilization Scale. Multiple linear regression was used to analyze the influencing factors.Results:Among 133 nurses with master's degree, the total scores of the EBP-COQ and the Barriers to Research Utilization Scale were (98.57±11.01) and (79.66±20.46) respectively. Multiple linear regression analysis showed that the influencing factors of evidence-based practice competence of nurses with master's degree included the working lives, positional titles, attending evidence-based nursing training, awareness to evidence-based practice, accessibility of research funding and resource conditions of evidence-based nursing ( P<0.05) . Conclusions:Nurses with master's degree in Henan Province have the intermediate level of evidence-based practice competence and positive attitudes to evidence-based practice, but their evidence-based knowledge and skills need to be improved. Nursing managers should pay more attention to evidence-based practice competence training of nurses with master's degree, provide many supportive resources and environments, promote evidence-based practice skills training so as to boost the development of specialist nursing practice.