1.Expression of a testis-specific gene 1700001022RIK in mice and its bioinformatic analysis.
Yu-chi LI ; Shou-ren LIN ; Man-ling LUO ; Huan GUO ; Han-wei WU ; Zhi-mao JIANG ; Yao-ting GUI
National Journal of Andrology 2015;21(5):391-395
OBJECTIVETo identify the expression characteristics of the 1700001022RIK (RIKEN cDNA 1700001022) gene in mice and explore its function by bioinformatic analysis.
METHODSUsing the expression profile of gene microarray, we detected the expression of a new testis-specific gene, 1700001022RIK, in mice. We analyzed its expression characteristics in the testis tissue and their changes in different developmental stages of the testis by RT-PCR, real-time RT-PCR, Western blot, and immunohistochemistry. We performed bioinformatic analysis using a bioinformatic software.
RESULTSThe 1700001022RIK gene was specifically expressed in the mouse testis in an age-dependent manner, most highly in the adult mice. The 1700001022RIK protein was mainly expressed in the spermatogonia, spermatocytes, and round spermatids of the adult mice. Bioinformatic analysis showed that the 1700001022RIK protein amino acid sequence had a high similarity in human and mice, which indicated that this gene was highly conserved in mammals.
CONCLUSION1700001022RIK is a testis-specific gene mainly expressed in the spermatogonia, spermatocytes, and round spermatids of seminiferous tubules, which might be involved in the regulation of spermatogenesis.
Age Factors ; Animals ; Blotting, Western ; Computational Biology ; DNA, Complementary ; Gene Expression ; Genomics ; Male ; Mice ; Molecular Chaperones ; genetics ; Seminiferous Tubules ; Spermatids ; Spermatocytes ; Spermatogenesis ; genetics ; Spermatogonia ; Testis
2.Research on Prevalence State of Children with Skeletal Fluorosis in Burning Coal Endemic Fluorosis in Zhijin County of Guizhou Province
shou-ying, WANG ; mao-juan, YU ; zhen, ZUO ; pei-ping, KANG ; xin-hua, LI ; xu-guang, CHEN
Journal of Applied Clinical Pediatrics 2004;0(08):-
Objective To explore the prevalence satate of children in burning coal endemic fluorosis in Zhijin county of Guizhou province, provide the scientific basis for the prevention of skeletal fluorosis.Methods One thousand six hundred and sixteen children in school under 16 years old that were sampled in cluster sampling were examined with dental fluorosis,X-ray in the type of burning coal pollution fluorosis areas of Zhijin county Guizhou province.Results Total prevalence rate of dental fluorosis was 96.42%,prevalence rate of skeletal fluorosis was 7.49%, constrictive skeletal fluorosis was main type in Zhijin county Guizhou province.Conclusion Prevalence states of dental fluorosis and skeletal fluorosis are still serious, more effectual preventive and control measure shall be used.
3.Expression characteristics of the Ccdc70 gene in the mouse testis during spermatogenesis.
Jian-bo CHEN ; Wen-zhong ZHENG ; Yu-chi LI ; Shou-ren LIN ; Zeng ZHANG ; Yong WU ; Zhi-mao JIANG ; Yao-ting GUI
National Journal of Andrology 2016;22(1):12-16
OBJECTIVETo investigate the expression characteristics of the gene of coiled-coil domain-containing protein 70 (Ccdc70) in the mouse testis and its potential role in spermatogenesis.
METHODSUsing expression profile microarray, we screened the mouse testis-specific gene Ccdc70, studied its expression characteristics in the mouse testis by RT-PCR, real-time PCR, Western blot and immunohistochemistry, followed by bioinformatic analysis of the Ccdc70 protein.
RESULTSThe Ccdc70 gene was expressed highly in the testis but lowly in the epididymis of the mice. The Ccdc70 protein was expressed mainly in the spermatocytes and round spermatids of the testis and in the epithelial cells of the epididymis. Bioinformatic analysis showed a structural domain in the Ccdc70 protein, which was highly conserved in mammalian evolution.
CONCLUSIONThe Ccdc70 gene is highly expressed in the mouse testis and mainly in the spermatocytes, round spermatids, and epididymal epithelial cells, which indicates that it is involved in the regulation of spermatogenesis and epididymal sperm maturation.
Animals ; Computational Biology ; Gene Expression Regulation, Developmental ; Male ; Mice ; Proteins ; genetics ; Spermatogenesis ; genetics ; Testis ; metabolism
4.Effects of Coal Burning Related Endemic Fluorosis on Body Development and Intelligence Levels of Children
shou-ying, WANG ; he-xi, ZHANG ; wei, FAN ; shi-jie, FANG ; pei-ping, KANG ; xin-hua, LI ; xu-guang, CHEN ; mao-juan, YU
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To study the effects of coal burning related endemic fluorosis on body development and intelligence levels of fluorosic children in Zhijin county.Methods One hundred and seventy-six fluorosic children and 50 healthy(without fluorostic teeth) children aging 7-12 years who were sampled in cluster sampling were examined for urine fluorosis,physical examination and intelligence tests in Zhijin county,with coal burning pollution related fluorosis.Results Physical development of sick children and the children in control group were in low levels. The intelligence levels and the leves of urine fluorosis in study group were lower than those in control group and there was a significant difference(P
5.Puerarin decreases lens epithelium cell apoptosis induced partly by peroxynitrite in diabetic rats.
Li-Na HAO ; Yi-Qun LING ; Xiu-Mei LUO ; Yu-Xiang MAO ; Qi-Yan MAO ; Shou-Zhi HE ; Yi-Ling LING
Acta Physiologica Sinica 2006;58(6):584-592
The present study was designed to observe if puerarin decreases lens epithelium cell (LEC) apoptosis induced partly by peroxynitrite (ONOO(-)). One hundred and eight rats were randomly divided into control group (n=36), streptozotocin (STZ) group (n=36) and STZ + puerarin group (n=36). The rats in the control group intraperitoneally (i.p.) received 0.5 ml of saline. The rats in STZ group and STZ + puerarin group received intraperitoneal injection of STZ (45 mg/kg). Three days later, the rats in STZ + puerarin group were given puerarin (140 mg/kg per day, i.p.). On days 20, 40 and 60 of the experiment, morphologic changes of lenses were observed with slit lamp. Then the animals were sacrificed for further analysis. The amount and percentage of apoptotic LECs were determined by flow cytometry. Nitrotyrosine (NT, the foot print of ONOO(-)) was examined by immunohistochemistry. Apoptosis-related genes (iNOS, etc.) were analyzed by gene array. The results showed that in the control group, all the lenses were clear. In STZ group, gradually severe opacity of the lens was observed on days 20, 40 and 60. But in STZ + puerarin group, mild opacity of the lens was observed on day 20 and more severe on day 40, but markedly decreased on day 60. In the control group, mild apoptosis of LECs was observed. In STZ group, time-dependent increase in apoptosis of LECs was observed. In STZ + puerarin group, mild apoptosis of LECs was observed on day 20, significantly increased on day 40, but markedly decreased on day 60. There was no expression of NT in the lens in the control group, but an increased expression of NT in STZ group. In STZ + puerarin group, mild expression of NT was observed on day 20, significantly increased on day 40, but markedly decreased on day 60. There was no expression of iNOS in the lens in the control group, but continuous up-regulation of iNOS expression in STZ group. In STZ + puerarin group, mild expression of iNOS was observed on day 20, significantly increased on day 40, but markedly decreased on day 60. Except the changes of iNOS related to NO production, the other apoptosis-related genes, including BCL-2 and SOD were down-regulated, while NF-kappaB and TNFR1-FADD-caspase signal transduction way were up-regulated in STZ group. The results were opposite in STZ + puerarin group and the control group. These findings show that NT is expressed in diabetic rat lens, which proves that LEC apoptosis in diabetic lens is partly induced by ONOO(-) which may be a new oxidative damage way to form cataract. Puerarin partly decreases LEC apoptosis induced by ONOO(-) and is a potential medicine for therapy of diabetic cataract. The mechanism of puerarin dealing with diabetic cataract may be related to its direct inhibition of LEC apoptosis and antagonism of ONOO(-) in diabetic rats.
Animals
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Apoptosis
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Cataract
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chemically induced
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Diabetes Mellitus, Experimental
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Epithelial Cells
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drug effects
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Isoflavones
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pharmacology
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Lens, Crystalline
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cytology
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Nitric Oxide Synthase Type II
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metabolism
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Peroxynitrous Acid
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Rats
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Tyrosine
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analogs & derivatives
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metabolism
6.Autophagy in fibrotic and postoperative remnant liver in rat.
Yi-lei MAO ; Rong-rong CHEN ; Hua-yu YANG ; Jing-chun ZHANG ; Yi-dan ZHANG ; Jian-hui MA ; Xin-ting SANG ; Xin LU ; Shou-xian ZHONG ; Jie-fu HUANG
Acta Academiae Medicinae Sinicae 2008;30(4):421-425
OBJECTIVETo evaluate the characteristics of autophagy in fibrotic and postoperative remnant liver.
METHODSMale Wistar rats were randomly divided into three groups: control group; fibrosis group, which received the solution of CCl4 in oil twice a week for 5 weeks; and hepatectomy group, which underwent 70% hepatectomy. Liver tissues and plasma were harvested 18 hours after the surgery. The rats' general conditions and plasma liver function were observed. Histopathological characteristics and regeneration were observed with microscope and transmission electron microscope. Qualitative analysis of autophagosome was made base on the data from transmission electron microscope.
RESULTSCompared with the control group, plasma total protein and albumin level significantly decreased in the fibrosis group (P < 0.01). Proliferating cell nuclear antigen (PCNA) index was 85%-95% in the fibrosis group. Plasma alanine aminotransferase and aspartate aminotransferase levels significantly increased in the hepatectomy group compared with the control group (P < 0.01), while the autophagical index significantly decreased in both the fibrosis group and hepatectomy group compared with the control group (-95%, P < 0.01; -19%, P < 0.05, respectively). PCNA index was 20%-30% in the hepatectomy group.
CONCLUSIONSAutophagy is weakened after fibrosis and hepatectomy, although it differs between these two processes. Proper regulation of autophagy may help facilitate the recovery of the residual liver function after hepatectomy.
Alanine Transaminase ; blood ; Animals ; Aspartate Aminotransferases ; blood ; Autophagy ; Disease Models, Animal ; Hepatectomy ; Humans ; Liver ; metabolism ; pathology ; physiopathology ; surgery ; Liver Cirrhosis ; metabolism ; pathology ; physiopathology ; surgery ; Male ; Proliferating Cell Nuclear Antigen ; metabolism ; Random Allocation ; Rats ; Rats, Wistar
7.Influence on the cytokines expression on hepatic tissue by inhibition the signal pathway of inflammatory mediators following extensive hepatectomy in rats.
Yi-lei MAO ; Zhuo YU ; Xin-ting SANG ; Xing LU ; Zhi-ying YANG ; Shou-xian ZHONG
Chinese Journal of Surgery 2005;43(1):6-9
OBJECTIVETo investigate the impact of AG490, a cytokine signaling inhibitor, on cytokine signaling pathway with phosphorylation levels of Janus kinase 2 (Jak2) and singal transducers and activators of transcription 3 (Stat3), and liver pro-/anti-inflammatory cytokine expressions.
METHODSRats were divided into two groups after surgery: control group, without treatment; AG490 group, with AG490 (1 mg.kg(-1).12 h(-1)) administration intraperitoneally, immediately and through 36 hs after the operation. Western blotting was used to detect the levels of phosphorylated Jak2 and Stat3. Semi-quantitative RT-PCR was employed to examine Interleukin-6 (IL-6) and Interleukin-10 (IL-10) expression.
RESULTSAt 8 h and 12 h post-operatively, the phosphorylation levels of Jak2 and Stat3 were significantly inhibited in the AG490 group when compared with the control group. The DNA levels of IL-6 in the liver of the AG490 group rat at the same time points were also decreased, whereas IL-10 levels markedly increased. These changes made the ratio of IL-6/IL-10 dropped significantly.
CONCLUSIONSAG490 ameliorates the overwhelming inflammatory response via a mechanism of blocking cytokine signaling transduction and consequently suppresses the ratio of pro-/anti-inflammatory cytokine expression, which exerts potential clinical implications of use of anti-inflammatory agents in hepatic surgery.
Animals ; Cytokines ; biosynthesis ; DNA-Binding Proteins ; metabolism ; Hepatectomy ; methods ; Interleukin-10 ; biosynthesis ; Interleukin-6 ; biosynthesis ; Janus Kinase 2 ; Liver ; drug effects ; physiology ; Male ; Protein-Tyrosine Kinases ; metabolism ; Proto-Oncogene Proteins ; metabolism ; Rats ; Rats, Sprague-Dawley ; STAT3 Transcription Factor ; Signal Transduction ; drug effects ; physiology ; Trans-Activators ; metabolism ; Tyrphostins ; pharmacology
8.Mechanisms of increased survival rate by administration of AG490 in rats following extensive liver resection.
Yi-lei MAO ; Zhuo YU ; Xin LU ; Xin-ting SANG ; Zhi-ying YANG ; Shou-xian ZHONG
Acta Academiae Medicinae Sinicae 2005;27(1):73-76
OBJECTIVETo study the mechanism by which AG490 improves the survival rate of rats following extensive liver resection.
METHODSThirty-eight rats were randomly divided into two groups after surgery: control group (n=10), without treatment; (2) AG490 group (n=28), with AG490 (1 mg x kg(-1) x 12 h(-1)) administrated intraperitoneally immediately and 36 hours after the operation. The survival rate was observed and the serum liver functions were measured.
RESULTSThe survival rates of control group and AG490 group were 0% and 25%. AG490 group had significantly better blood glucose and aminotransferase levels (P < 0.05) than control group; serum bilirubin levels significantly decreased 48 hours after the operation. Serum protein levels in both two groups had slow decrease but without statistical significance (P > 0.05).
CONCLUSIONSAG490 can significantly increase the survival rate of rats following extensive liver resection. Such a benefit mainly results from the protection towards residual liver function rather than from the promotion of liver regeneration.
Alanine Transaminase ; blood ; Animals ; Aspartate Aminotransferases ; blood ; Bilirubin ; blood ; Blood Glucose ; metabolism ; Enzyme Inhibitors ; pharmacology ; Hepatectomy ; methods ; Male ; Protein-Tyrosine Kinases ; antagonists & inhibitors ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Survival Rate ; Tyrphostins ; pharmacology
9.Incidence and mortality of larynx cancer in China during 2003-2007
Ling-Bin DU ; Wei-Min MAO ; Wan-Qing CHEN ; Si-Wei ZHANG ; Chuan-Ding YU ; Rong-Shou ZHENG ; Qing-Min XIA ; Xiang-Hui WANG
Chinese Journal of Epidemiology 2012;33(4):395-398
Objective To analyze the patterns of incidence and mortality on larynx cancer in China.Methods Data from 32 Cancer Registries in China were examined,sorted,and analyzed by the National Cancer Registry,to obtain the crude,Chinese national and world age- standardized rates (ASR) of incidence and mortality and their trends.Results The crude incidence and mortality rates of larynx cancer wcrc 2.04/105 and 1.06/105 in China during 2003-2007.The rates were higher in males than those in females,and also higher in urban areas than those in rural areas.The highest Chinese ASRs of incidence and mortality of larynx cancer in 32 cancer registries in China were 2.08/105 in Zhongshan city,Guangdong province,and 1.58/105 in She county,Hebei province respectively.The trend of incidence and mortality of larynx cancer was stable from 2003 to 2007.Conclusion Although both the incidence and mortality of larynx cancer in China were still in low level,comprehensive measures should be carried out to prevent the increase on both the incidence and mortality of larynx cancer.
10.Expressions of survivin and nm23 and the relationship among the expressions and the axillary lymph node metastasis in breast cancer.
Jie MAO ; Jian HAI ; Heng-Ping SHU ; Yu-Hui WU ; Hui-Yin OU ; Sha-Yang LUO ; Shou-Man WANG
Journal of Central South University(Medical Sciences) 2005;30(3):312-334
OBJECTIVE:
To investigate the expressions of Survivin protein and nm23 protein and the relationship among the expressions and axillary lymph node metastasis in breast cancer.
METHODS:
The expression of Survivin and nm23 in 80 cases of breast cancer tissues were detected by immunohistochemistry SP method, and their correlation with axillary lymph node metastasis and 5-year disease free survival (DFS) were analysed.
RESULTS:
Survivin protein positive expression rate was 68.75% (55/80) in breast cancer tissues, which had positive correlation with the axillary lymph nodes metastasis but negative correlation with 5 years FS (P < 0.05); nm23 protein expression had negative correlation with the axillary lymph nodes metastasis but positive to 5 years FS (P < 0.05). Survivin and nm23 proteins expression had no obvious correlation with the breast cancer pathology type, patient age and clinical stage (P > 0.05).
CONCLUSION
The anti-apoptosis effect of Survivin protein and the anti-metastasis effect of nm23 protein may be important in the occurrence and advancement of breast cancer, suggesting that it may be a new indicator of prognostic and judgement in breast cancer.
Adult
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Aged
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Axilla
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Biomarkers, Tumor
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Female
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Humans
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Inhibitor of Apoptosis Proteins
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Lymph Nodes
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pathology
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Lymphatic Metastasis
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Mastectomy
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Microtubule-Associated Proteins
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biosynthesis
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genetics
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Middle Aged
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NM23 Nucleoside Diphosphate Kinases
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Neoplasm Proteins
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biosynthesis
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genetics
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Nucleoside-Diphosphate Kinase
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biosynthesis
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genetics
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Prognosis
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Retrospective Studies
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Survivin