1.Mitochondrial retrograde signaling: a new option for the treatment of mitochondrially related diseases
Yu JIANG ; Shou-bao WANG ; Guan-hua DU
Acta Pharmaceutica Sinica 2021;56(3):661-668
Mitochondria play a key role in cell metabolism. In addition to synthesizing ATP, they also participate in many physiological and pathological processes, including apoptosis, inflammation, oxidative stress, neuronal disease, tumor development, and aging. Most gene transcription of mitochondrial proteins occurs in the nucleus, so the biogenesis of mitochondria and the maintenance of mitochondrial homeostasis mainly depend on the expression of nuclear genes (nDNA) and mitochondria-nucleus interactions. Conversely, mitochondria can affect the expression of nuclear genes through nuclear transcription factors, a process called mitochondrial retrograde signaling. This review summarizes the research progress on mitochondria-nucleus retrograde signaling and its regulation, including the ways by which mitochondria regulate nuclear genes and affect biological processes, and discusses new strategies for the treatment of diseases that involve mitochondrial retrograde signaling in disease pathology.
2.Analysis of Application of Placebo Acupuncture in Recent 5 Years' Domestic Randomized Controlled Trials
yan Shou WANG ; Hui WANG ; Fei CAI ; jie Bao HAN
Shanghai Journal of Acupuncture and Moxibustion 2017;36(10):1269-1276
Objective To summarize the domestic design for placebo acupuncture and the placebo effect and explore an ideal design for placebo acupuncture.Method Three large Chinese databases CNKI, Wan Fang and Vip were searched to systematically review the application of placebo acupuncture in randomized controlled trials published at home in recent 5 years (2011.01—2016.11).Results and conclusions A total of 48 articles were included according the inclusion and exclusion criteria. An analysis was made of the kinds of diseases for acupuncture study, the methods of designing placebo acupuncture, the effects of placebo acupuncture and comparisons with foreign placebo acupuncture. Pain syndrome and insomnia were the main kinds of diseases for acupuncture study, accounting for 10 and 8 articles respectively. Acupuncture needles were the main kind of needles for placebo acupuncture, accounting for 66.6%. Sites beside acupoints were the main positions selected for placebo acupuncture, accounting for 58.3%. Not penetrating the skin predominated in the depth of needle insertion, accounting for 37.5%. The kinds of needles for placebo acupuncture were fewer than abroad. The effects of placebo acupuncture were not superior to those of acupuncture.
3.Effect and mechanism of total flavonoids of bugloss on rats with myocardial ischemia and reperfusion injury.
Xiao-Na XU ; Zi-Ran NIU ; Shou-Bao WANG ; Yu-Cai CHEN ; Li GAO ; Lian-Hu FANG ; Guan-Hua DU
Acta Pharmaceutica Sinica 2014;49(6):875-881
This study is to investigate the effect of total flavonoids of Uygur medicine bugloss (BTF) on rats with myocardial ischemia/reperfusion injury, and to explore the mechanisms by which it acts. Left anterior descending (LAD) coronary artery in rats was occluded for 30 min followed by 4 h reperfusion. Meanwhile, BTF dissolved in saline was administered intraperitoneally at dosage of 10, 30 and 50 mg x kg(-1). Electrocardiograph, infarction index, serum myocardial enzymes and heart function were determined to evaluate the effect of BTF. Some other observations were carried out to explore whether inhibiting inflammation and apoptosis is involved in the mechanisms underlying BTF. Our results showed that in ischemia/reperfusion injured rats BTF could dose-dependently reduce myocardial infarction index and myocardial enzyme leakage, and enhance heart function, indicating that it possesses significant cardio protection. ELISA analysis showed that BTF could decrease the content of myocardial inflammatory cytokines such as IL-1beta, IL-6 and TNF-alpha. Western-blotting confirmed that BTF could increase the expression of anti-apoptotic protein Bcl-2 and reduce the expression of proapoptosis protein Bax. Further more, the phosphorylation level of PI3K and Akt was upregulated by BTF treatment. BTF can protect rat against myocardial ischemia/reperfusion injury. Anti-inflammation and inhibition of apoptosis through upregulating PI3K/Akt signal pathway may contribute to the protective effect of BTF.
Animals
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Apoptosis
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Apoptosis Regulatory Proteins
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Boraginaceae
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chemistry
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Flavonoids
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pharmacology
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Heart
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Interleukin-6
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Myocardial Infarction
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Myocardial Reperfusion Injury
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drug therapy
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Myocardium
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Phosphatidylinositol 3-Kinases
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Phosphorylation
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Protective Agents
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Proto-Oncogene Proteins c-akt
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Rats
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Signal Transduction
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Tumor Necrosis Factor-alpha
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bcl-2-Associated X Protein
4.Survey of study on the extraction, purification and determination methods of glycyrrhizic acid in Licorice.
Shou-ping LU ; Qun SUN ; Jian-hua WANG ; Bao-qi SUN
China Journal of Chinese Materia Medica 2006;31(5):357-360
Extraction, purification and determination methods of glycyrrhizic acid in Licorice are surveyed in this paper. The extracting efficiency of dilute ethanol solvent for glycyrrhizic acid is higher than water extraction, and ammonia appended to ethanol solution can increase the effect of extraction. Ultrasound method, microwave-assisted method and supercritical CO2 fluid extraction are more effective than the conventional techniques, due to the short extraction time, low consumption of solution and energy. The modem analytical methods such as TLCs, HPLC and CE can determine the content of glycyrrhizic acid rapidly and exactly.
Ammonia
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Chromatography, High Pressure Liquid
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Chromatography, Supercritical Fluid
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Chromatography, Thin Layer
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Electrophoresis, Capillary
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Ethanol
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Glycyrrhiza
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chemistry
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Glycyrrhizic Acid
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analysis
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isolation & purification
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Microwaves
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Plants, Medicinal
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chemistry
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Technology, Pharmaceutical
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methods
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Ultrasonics
5.Clinical study:endovascular embolization of intracranial aneurysm with coils
Jin-Ning SONG ; Shou-Xun LIU ; Gang BAO ; Xiao-Bin LIU ; Xiao-Dong ZHANG ; Tuo WANG ; Wan-Fu XIE ;
Chinese Journal of Radiology 2001;0(08):-
Objective To summarize the technique and managements of complications in endovascular embolization on intracranial aneurysm with Guglielmi detachable coils(GDC),and to evaluate the effect of the treatment.Methods One hundred and thirty six cases with Aneurizym were treated using GDC to embolize the aneurismal sac via femoral artery approach.Results One hundred and thirty six aneurysms were cured.Of them 132 cases recovered clinically,4 patients died.The mortality was 2.9%. The sac of 123 aneurysms were embolizied at 100%,8 cases with 95% embolization,5 with 90% embolization.3 aneurysms reptured during the embolization,cerebral vasospasm happened in 7 eases. microcoil escaped in 2 case.Three recurring cases were cured after second GDC embolization.The technique-related complications occured in 13 cases.No re-bleeding occurred during the 6 to 54-month follow-up.Conclusion Endovascular embolization of intracranial aneurysm with coils is a safe and effective treatment for intracranial aneurysm.Advances in Techniques and treating the complications correctly would decrease the complications and improve future outcomes.
6.TME or TSD with pelvic autonomic nerve preserving operation for 247 patients with advanced rectal cancer
Chun-Bao ZHAI ; Yong-Gang WANG ; Li-Jun TIAN ; Li-Yun NIU ; Jian-Yong YANG ; Shou-Ren JIA ;
Cancer Research and Clinic 2006;0(11):-
Objective To investigate effects of rectal cancer to undergo total mesorectal excision (TME)or three space dissection(TSD)with pelvic autonomic nerve preservation(PANP).Methods TME or/ and TSD was applied in 247 Patients with advanced rectal cancer in which 185 cases (74.9 %) underwent PANP(Group P)including TME-PANP(Group Pro)139 cases and TSD-PANP(Group Ps)46 cases.The other 62 cases underwent none-PANP(Group P-)due to tumor invasion.Results There were no death cases for operation inall patients.Group Pm was better than Group Ps in the operation time and the difficulty of proce- dure(P0.05).Conclusion The procedure with TME to preserve pelvic autonomic nerves adapts to the majority of rectal cancer patients.TSD procedure is more complex than TME.Statistically,the survival differ- ence between Patients with TSD and with TME is no defective.The survival time is determined to the tumor's earlier diagnosis and therapy.
7.Puerarin reduces oxidative damage to vascular endothelial cells by improving mitochondrial respiratory function
Shu-chan SUN ; Di-fei GONG ; Tian-yi YUAN ; Shou-bao WANG ; Lian-hua FANG ; Guan-hua DU
Acta Pharmaceutica Sinica 2022;57(5):1352-1360
This study investigated the effect of puerarin on human umbilical vein endothelial cells (HUVEC) injured with hydrogen peroxide (H2O2). HUVEC were divided into three groups: a control group, a model group (H2O2 400 μmol·L-1) and a puerarin-treated group (3, 10, 30 and 100 μmol·L-1). HUVEC were cultured with varied concentration of puerarin for 2 h and treated with H2O2 for another 24 h. Cell proliferation was detected by a CCK-8 assay. The mitochondrial membrane potential was measured by a JC-1 fluorescent probe. A transwell chamber assay was adopted to observe cell migration ability. Mitochondrial respiratory function was measured in a two-chamber titration injection respirometer (Oxygraph-2k). The expression of interleukin-1
8.Puerarin protects against myocardial ischemia/reperfusion injury by suppressing NLRP3 inflammasome activation via TLR4/Myd88/NF-κ B pathway in rats
Dan-shu WANG ; Liu-yan YAN ; Shu-chan SUN ; Yu JIANG ; Su-yue YIN ; Shou-bao WANG ; Lian-hua FANG ; Guan-hua DU
Acta Pharmaceutica Sinica 2021;56(5):1343-1351
This study was to investigate the protective effects of puerarin on myocardial ischemia/reperfusion (MI/R) injury and the underlying mechanism. The MI/R-model was established by ligating the left anterior descending artery (LAD) for 60 min followed by 24 h reperfusion, puerarin (10, 30, and 100 mg·kg-1) was orally administered 20 min before reperfusion. Cardiac function, myocardial infarct index, cardiac damage markers, inflammatory cytokines, and apoptosis index were measured to evaluate the protective effects of puerarin on MI/R injury. The activation of Nod-like receptor protein 3 (NLRP3) inflammasome and Toll like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor kappa B (NF-
9.Vasorelaxant effect and mechanisms of compound reserpine and triamterene tablets on the isolated thoracic aorta rings
Di-fei GONG ; Ran-ran WANG ; Tian-yi YUAN ; Shou-bao WANG ; Jun-ke SONG ; Lian-hua FANG ; Guan-hua DU
Acta Pharmaceutica Sinica 2022;57(11):3339-3344
This study aimed to evaluate the vasorelaxant effect and mechanisms of compound reserpine and triamterene tablets (CRTTs) and its component triamterene on isolated rat thoracic aorta rings. Isolated rat thoracic aorta rings pre-contracted by high potassium or norepinephrine (NE) were used to evaluate the vasodilatory effect of CRTTs and its component triamterene. The mechanisms concerning endothelium, potassium channels and calcium channels were studied through the interventions of several tool drugs. Animal welfare and experimental procedures followed the requirements of the Laboratory Animal Management and Animal Welfare Ethics Committee of the Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College. The results showed that both CRTTs and triamterene had potent relaxant effect on KCl and NE pre-contracted vessels. Triamterene showed partial endothelium dependency, and
10.Arteriovenous neoadjuvant FLEOX chemotherapy with nutritional support improves the resectable rate for advanced gastric cancer.
Guo-Li LI ; Yang BAO ; Jun JIANG ; Chao-Gang FAN ; Zhi-Ming WANG ; Ning LI ; Jie-Shou LI
Chinese Journal of Gastrointestinal Surgery 2008;11(5):428-431
OBJECTIVETo summarize the resectable rate of advanced gastric cancer characterized by abdominal lymph node metastases with the arteriovenous neoadjuvant FLEOX chemotherapy plus appropriate nutritional support.
METHODSArteriovenous neoadjuvant FLEOX chemotherapy was administered to 50 patients of gastric cancer with advanced abdominal lymph node metastases. Of the 50 patients, 42 had never received any previous therapy and preoperative CT scanning revealed unresectable tumors because of advanced lymph node (station No.3, 7, 9, 12) or distant lymph node (No.14,16) metastases. The other 8 were characterized with relapse of severe lymph node metastases or with unresectable lymph node metastases demonstrated by exploratory laparotomy. Arteriovenous neoadjuvant FLEOX chemotherapy was conducted as follows: from day 1 to day 5, 5-FU 370 mg/m(2) and leukovorin 30 mg/kg intravenously, at day 6 and day 20, CDDP 70 mg/m(2) and epotoside 70 mg/m(2) intraarterially. This FLEOX regimen was repeated every five weeks for two or three courses. Out of the 50 patients,12 malnutritional cases received parenteral and/or enteral nutritional support according to the nutritional condition. Arteriovenous neoadjuvant FLEOX chemotherapy was then administered after the improvement of nutritional state. Their nutritional support methods were adapted to their chemotherapy as well.
RESULTSAll the patients' general conditions and symptoms were improved significantly. For the 50 cases, the imageological and histological response rate (CR+PR) was 84.0%, and curative resection rate was 78.0%. Thus, 39 patients underwent subtotal or total gastrectomy, even combined organ resection, with D(2)+alpha or D(3) lymphadenectomy. Despite neoadjuvant chemotherapy, all malnutritional cases had significant weight gain after nutritional support, and other nutritional indexes,such as serum albumin, also resumed to normal.
CONCLUSIONArteriovenous neoadjuvant FLEOX chemotherapy proves favorable therapeutic effect for gastric cancer with advanced abdominal lymph node metastases, and downstages inoperable metastatic lymph nodes for radical operation. This combined modality regimen and nutritional support may play an important role in the treatment of advanced gastric cancer.
Adult ; Aged ; Chemotherapy, Adjuvant ; Female ; Gastrectomy ; Humans ; Male ; Middle Aged ; Neoadjuvant Therapy ; methods ; Nutritional Support ; Stomach Neoplasms ; surgery ; therapy