Objective To investigate the prevalent characteristics of children with viral pneumonia in different areas and years.Methods The viral antigen of cells of nasopharyngeal secretions was detected by the method of alkaline phosphatasea anti-alkalinephhosphatase in viral pneumonia children in different areas and years.Results Among 627cases , there were 290 positive cases and 14 cases were infected by 2 viral strains. Three hundred and four positive viral strains,71 cases (23.35%) were RS virus infection, 47 cases (15.46%) were AD virus infection, 74 cases (24.34%) were FLU-A virus infection,50 cases (16.44%) were FLU-B virus infection, 47 cases (15.46%) were PIV1,3 virus infection, and 15 cases (4.93%) were PIV2 virus infection. But in different areas, the kind of the viral strain of the highest infection rate was different. The viral detection rate in autumn and winter was the highest and in summer was the lowest. Conclusions FLU-A virus is the main etiological agent of children viral pneumonia,but the main etiological agent in different areas is different. In autumn and winter, the viral infection rate is high. The chance of viral infection of lower respiratory tract of big children is reduced.

Objective To discuss the applied value of multislice spiral CT(MSCT) in diagnosing and localizing the embedded supernumerary teeth. Methods A total of 43 cases with the embedded supernumerary teeth were undergone MSCT using shaded surface display(SSD), minimum intensity projection(MIP) and multiplanar reformation(MPR) to present the form and location of the embedded supernumerary teeth and to determine the surgical style.The result of image was retrospectively analysed after operation.Results The reconstruction images,could clearly show the dental surface image including crown, root, neck and root bifurcation, labial or palatal location, eruption orientation and relation with dentition in anterior maxilla. The position of 52 embedded supernumerary teeth in 43 patients were completely confirmed by surgery with accuracy of 100%. All patients were well healed with no infection and injury to nearby teeth. Conclusion The Embedded supernumerary teeth in bone can be detected by MSCT and MSCT can be used as an important examination technique before surgical and orthodontic treatment.

Objective To investigate the efficacy of patient-controlled epidural analgesia(PCEA)with ropivacaine plus fentanyl for postoperative analgesia after suprapubic prostatectomy(SPP).Methods Thirty-six ASAⅡorⅢpatients aged 62-78 yrs weighing 52-84 kg undergoing SPP were randomly divided into 3 groups with 12 patients in each group:groupⅠreceived PCEA with 0.12% ropivacaine and fentanyl 1?g?ml~(-1);groupⅡreceived PCEA with 2% ropivacaine and fentanyl 1?g?ml~(-1) and groupⅢreceived patient-controlled intravenous analgesia(PCIA)with morphine 0.2 mg?ml~(-1).Postoperative pain was assessed using VAS(0=no pain,10= worst pain).The degree of motor blockade(modified Bromage scale)was evaluated at 6,24,48 and 72 h after operation(T_(1-4)).Bladder spasm episodes were recorded.Side effects including nausea,vomiting and pruritus and the total number of RBC in rinse solution were also recorded.Results(1)Bromage score(0=no motor block,3 =inability to flex ankle joint)was significantly higher in groupⅠandⅡthan in groupⅢat T1(6 h after op.), but significantly lower in groupⅠandⅢthan in groupⅡat T_(2-4)(24-72 h after operation).(2)VAS score(0= no pain,10=worst pain)was not significantly different among the 3 groups without bladder spasm but significantly higher in groupⅢthan in other 2 groups during episodes of bladder spasm.(3)The number of bladder spasm episodes was not significantly different among the 3 groups within 6h after operation,but was significant larger in groupⅢthan in the other two groups during 6-72 h after operation.During 6-24 h after operation more bladder spasm attacks occurred in groupⅠthan in groupⅡ.(4)The incidence of side effects was significantly lower and the total number of RBC in rinse solution was significantly smaller in groupⅠandⅡthan in groupⅢConclusion PCEA with 0.12% or 2% ropivacaine plus fentanyl can effectively reduce the number of bladder spasm attack and postoperative bladder bleeding.Different concentrations of ropivacaine should be used for PCEA during different postoperative periods.

Angioplasty, Balloon, Coronary ; Coronary Occlusion ; diagnostic imaging ; therapy ; Fluoroscopy ; adverse effects ; Humans ; Male ; Middle Aged ; Radiodermatitis ; etiology ; Treatment Failure

OBJECTIVE: To establish a method of fingerprint position, sample transfer and fingerprint DNA extraction in contact samples. METHODS: Sixty-six cases were visualized by 502 glue fingerprint fumigation. Two methods, ordinary wipe and acetone wipe, were used to transfer cast-off cells of fingerprints from testing samples, respectively. DNA was extracted and purified by ultramicro magnetic bead kit. The data was resolved on genetic analysis after amplification. RESULTS: In 33 samples, 30 samples got better STR analysis by acetone wipe method. The peak range was 1,000-4,000 RFU and peak shapes were equable. It was hard to get ideal STR typing by ordinary wipe method. CONCLUSION The samples are visualized by 502 glue fingerprint fumigation and the case-off cells are transferred by acetone wipe method. The method shows better STR analysis result, which might be a better method for forensic science practice.
Adhesives ; DNA/isolation & purification* ; DNA Fingerprinting/methods* ; Forensic Medicine ; Fumigation/methods* ; Humans

Cardiovascular Diseases ; Humans ; Induced Pluripotent Stem Cells

Respiratory Protective Devices

Objective: To investigate the effect of survivin and bcl-2 antisense oligodeoxynucleotides ( AsODN) combined transfection on the human gallbladder carcinoma cell line GBC-SD in vitro. Methods: Survivin and Bcl-2 protein expressions were detected by immunohistochemical method; Cultured cells were divided into 4 groups: Nomal control group, survivin antisense observed group, bcl-2 antisense observed group and combined group. After transfected for 24 h, the expression of survivin mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Cell morphological changes were observed under electron microscopy. Apoptosis index (AI) was examined by flow cytometry; Inhibitory rate (IR) was determined by the colorimetri MTT cell viability and proliferation assay. Results: Survivin and Bcl-2 protein were highly expressed in gall bladder carcinoma cells; The expression of survivin mRNA was decreased 47. 8%. Abnormal morphological changes of cells were observed in the three AsODN transfection groups; The AI in survivin antisense observed group,bcl-2 antisense observed group,and combined group was 11.38%?3.91% , 9.26%?4.15% , 28.45%?6.34% respectively and significantly higher than the nomal control group (P

Background and purpose. Chemotherapy is a standard treatment for patients with advanced stage non-small cell lung cancer (NSCLC) However, platinum based chemotherapeutic regimen haves high toxicity profile to normal tissues. Lobaplatin (LBP) is a new third-generation antitumour platinum drug. Studies abroad have shown that Iobaplatin has an anticancer activity similar to cisplatin with better tolerance and is more effective for those who are resistant to cisplatin. This study was aimed to observe the effectiveness and toxicities of lobaplatin combined with vinorelbine (NVB) for the treatment of advanced NSCLC. Methods: Sixty-four patients pathologically diagnosed as clinical stage Ⅲ_B-Ⅳ NSCLC who did not receive treatment before, were randomly assigned to two groups. NL group (NVB+LBP): LBP at a dose of 30 mg/m~2 intravenous infusion on day 1, and NVB at a dose of 25 mg/m~2 intravenous infusion on days 1 and 8. NP group (NVB+DDP): Cisplatin (DDP) at a dose of 30 mg/m~2 intravenous infusion on day 1, 2 and 3 and NVB at a dose of 25 mg/m~2 intravenous infusion on days 1 and 8. Treatments were repeated every 21-28 days for 3 cycles. Results: For 34 patients of NL group, there were CR (1 cases), PR(13 cases), NC(15 cases), and PD(5 cases). The overall response rate (RR) was 41.2%, disease control rate (DCR) was 85.3%. In NP group: RR was 43.3%, DCR was 83.3% (X~2=0.05, P>0.05). Median overall survival time was 8.6 months and 8.9 months for NL group and NP group, respectively. The main toxicities in NL group were myelosuppression. Digestive toxicity such as anorexia, nausea,vomiting were less than those in the NP group (X~2=7.43, P<0.05), Peripheral hour,toxicity, serious liver and renal toxicity were not observed in NL group. Conclusion: Compared with cisplatin plus vinorelbine, domestic lobaplatin with vinorelbine yielded similar efficacies for NSCLC, but had less toxicity and well tolerate.

AIM: To determine coumarin and flavonoid in Compound Buguzhi Sustained-Release Tablet(Fructus Psoraleae,etc). METHODS: HPLC method was developed.The separation was performed on an ODS column with the mobile phase of CH_3OH-H_2O.The flow rate was 1 mL/min and the detection wavelength was 320 nm. RESULTS: The result showed that there was good linear relation between the area and the concentration of psoralen、isopsoralen、isobavachin、bavachin、corylin、corylifolinin、bvachinin、psoraidin and bavachalcone within 0.4-152.0,26.4-132.0,13.6-66.0,27.2-136.0,31.2-156.0,48.8-244.0,50.4-252.0,36.0-180.0,(8.8)-44.0 ?g/mL.The average recovery of psoralen and bavachin is 98.7% and 97.6%. CONCLUSION: This method is convenient,reliable,and can be used to control the quality of Compound Buguzhi Sustained-Release Tablet.