1.Determination of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis.
Zhi-hong XIN ; Hai-le MA ; Shou-yi WU ; Chun-hua DAI
Acta Pharmaceutica Sinica 2003;38(11):843-845
AIMTo establish a method for determinate of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis.
METHODSThe characteristic absorptive wavelength of hippuric acid determined by ultraviolet spectrophotometer is 228 nm. The method employed a melted capillary column, 50 mmol.L-1 phosphoric acid (pH 8.3) buffer solution, inject pressure 4.8 kPa, inject time 3 s, separation voltage 20 kV and detection wavelength 228 nm.
RESULTSThe reactant and resultant was separated completed within 7 min. IC50 of captopril was 0.019 mumol.L-1. Captopril is a competitive inhibitor, which was proved by enzyme reaction dynamics.
CONCLUSIONThe method was shown to be accurate, simple and rapid and can be used for determination of the inhibitory activity of captopril.
Angiotensin-Converting Enzyme Inhibitors ; pharmacology ; Captopril ; pharmacology ; Electrophoresis, Capillary ; methods ; Hippurates ; analysis ; Peptidyl-Dipeptidase A ; metabolism
2.Analysis of metabolites of daphnetin in the intestinal wall of rats by liquid chromatography and quatrupole-time of flight mass spectrometry.
Jin-jun SHAN ; Hai-shan DENG ; Hong-mei WEN ; Hao WU ; Shou-chuan WANG ; Liu-qing DI
Acta Pharmaceutica Sinica 2011;46(11):1366-1369
In this study, daphnetin and its major metabolites in the intestinal wall of rats were identified by liquid chromatography and quatrupole-time of flight mass spectrometry. Perfusion fluid of duodenum, jejunum, ileum and colon were collected separately for 2 hours from the rat intestine following perfusion with daphnetin. The metabolites of daphnetin in the perfusion fluid of different intestine segments were analyzed by the liquid chromatography and quatrupole-time of flight mass spectrometry. It is shown that the parent drug daphnetin and four metabolites were found in the perfusion fluid of duodenum, jejunum and ileum. However, no metabolites were found in the colon. Among the four metabolites, two daphnetin sulfates (m/z 257) were first discovered as the phase II metabolites of daphnetin in rats, which revealed a new way of daphnetin metabolism in rats.
Animals
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Chromatography, High Pressure Liquid
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Colon
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metabolism
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Duodenum
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metabolism
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Ileum
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metabolism
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Intestines
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metabolism
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Jejunum
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metabolism
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Male
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Perfusion
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Spectrometry, Mass, Electrospray Ionization
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Umbelliferones
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metabolism
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pharmacokinetics
3.Study on the mechanisms of cupping therapy.
Shou-Hai HONG ; Fei WU ; Xuan LU ; Qing CAI ; Yi GUO
Chinese Acupuncture & Moxibustion 2011;31(10):932-934
Computerized literature searches are performed for articles of the mechanism of cupping therapy in the past 10 years. The mechanism of action of local negative pressure applied to human body is studied in terms of specific changes in local tissue structure, stretch to the nerve and muscle, increasing blood circulation and causing autohemolysis. This paper aims to explain the mechanism of cupping therapy according to modern science.
Acupuncture Therapy
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methods
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Humans
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Medicine, Chinese Traditional
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methods
4.Association of polymorphism in alpha-adducin gene with antihypertensive effect of Hydrochlorothiazide.
Shou-ling WU ; Dong-qing LI ; Hong-fen LI ; Qing YU ; Yun LI ; Hai-yan ZHAO
Chinese Journal of Cardiology 2005;33(10):880-884
OBJECTIVETo explore the association between G614T single nuclear polymorphism (SNP) of the alpha-adducin gene and the antihypertensive effect of hydrochlorothiazide (HCTZ) in essential hypertensive (EH) patients.
METHODSEight hundred twenty nine EH patients were given 12.5 mg HCTZ/d for six weeks. Alpha-adducin gene G614T SNP in the tenth exon was determined by PCR-RFLP in 754 patients with complete records. All the patients were grouped according to TT, GT and GG genotypes.
RESULTSAfter 6 weeks of HCTZ treatment, the decreases in DBP and MAP of patients carrying 614T allele of alpha-adducin were significantly greater than that of those carrying GG homozygotes (P < 0.05). The decreases in SBP and MAP were significantly greater in patients with the TT genotype as compared with GT or GG genotype (P < 0.05). The effective rate of BP fall by HCTZ was higher in patients with TT genotype than those with GT or GG genotype (P < 0.05). Multivariate stepwise regression analysis showed that the TT genotype and the baseline SBP were the two major predictors affecting the decrease in SBP.
CONCLUSIONThe present study suggests that the alpha-adducin G614T polymorphism is associated with the antihypertensive effect of HCTZ, which is more effective in patients with TT genotype.
Adult ; Aged ; Aged, 80 and over ; Antihypertensive Agents ; therapeutic use ; Blood Pressure ; Calmodulin-Binding Proteins ; genetics ; Female ; Humans ; Hydrochlorothiazide ; therapeutic use ; Hypertension ; drug therapy ; genetics ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Single-Blind Method ; Treatment Outcome
5.Gynecomastia in 38 children: etiology and treatment.
Jian HAI ; Zhao-Yun WU ; Jie MAO ; Lei GUO ; Li-Li TANG ; Shou-Man WANG ; Yan LI
Chinese Journal of Contemporary Pediatrics 2010;12(6):433-435
OBJECTIVETo summarize the etiology and treatment of gynecomastia in male children.
METHODSThe clinical data of 38 boys with gynecomastia at ages of 2-14 years were retrospectively studied.
RESULTSIn the 38 cases, 17 cases were identified as adolescent breast hyperplasia, 2 cases were relevant to primary disease, 4 cases were caused by ingestion of drugs containing hormone, and 15 cases did not show identifiable causes and were diagnosed as idiopathic gynecomastia. For the 3 children with breast development in B3 stage, oral rupixiao was administered (1.34 g, tid) for one month. For 16 children at ages of over 12 years with breast development in B2 stage and with obvious clinical symptoms, oral rupixiao was administered (1.34 g, tid) for 3-5 days. The other patients did not receive drug treatment. In a one month to one year follow-up, most of the patients recovered well.
CONCLUSIONSThe etiology of gynecomastia in male children includes adolescent breast hyperplasia, ingestion of drugs containing hormone and secondary causes. Most gynecomastia can be attributed to physiological reasons. Only a few children with obvious clinical symptoms need drug treatment.
Adolescent ; Child ; Child, Preschool ; Gynecomastia ; etiology ; therapy ; Humans ; Male ; Retrospective Studies
6.Application of percutaneous needle aspiration biopsy by the simulator guided to the diagnosis for pulmonary focus in coal miners' pneumoconiosis.
Si-hai LIU ; Cheng-dong QI ; Wen-shou XU ; Rui-xia ZHU ; Qin YAN ; Wen FENG ; Rong-xia SUN ; Yan-fang ZHANG ; Xiao-fu WU ; Zheng-chuan FU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(1):72-73
Aged
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Biopsy, Needle
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methods
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Coal Mining
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Humans
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Lung
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pathology
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Lung Neoplasms
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complications
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diagnosis
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Middle Aged
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Pneumoconiosis
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complications
7.Effects of Long-term Treatment with Hydrochlorothiazide Combined Spironolactone or Captopril on Left Ventricular Hypertrophy in Hypertensive Patients
Ai-Jun XING ; Dong-Xian LI ; Xin DU ; Shou-Ling WU ; Hai-Yan ZHAO ; Li-Ming LING ; Dong-Qing LI ; Zheng-Xin CAO ; Gui-Ling WANG ; Qing YU ;
Chinese Journal of Hypertension 2007;0(05):-
0.05);2)After 12,24,36 months' treatment,BP was decreased significantly in each group (P0.05).Conclusion Both combined spirono- lactone/HCTZ and captopril/HCTZ significantly reduced BP and LVMI or LVMI and the maguitude of reduction was further enhanced after prolonged treatment.
8.Study on Stability of Mixture Reference Solution of Strychni Alkaloids
Fei-Fei WANG ; Shou-Hai WU ; Ming-Juan WANG ; Yu-Mei ZHANG ; Shuang-Cheng MA
Journal of Guangzhou University of Traditional Chinese Medicine 2018;35(2):328-332
Objective Brucine and strychnine monomer reference substance as extremely toxic substance had potential threat during transportation and utilization. In this study we investigated the homogeneity, stability and assignment accuracy of the mixture reference solution of strychnine and brucine, so as to provide reference for the quality control of extremely toxic chemical reference substances for Chinese medicine. Methods Following the assay in Chinese Pharmacopoeia volume I (2015), we prepared the mixture reference solution of brucine and strychnine, and investigated the solvents and the concentration of mixutre reference solution. The stability test lasted for 12 months. F-test was used for heterogeneity assay. Three researchers were involved for collaboration. Results Methanol and chloroform solution were selected as the solvents for the stability test. Results showed the difference was not statistically significant among various mixture solutions. The results of value assignment were 0.14 mg/mL for strychnine (sR = 0.5%)and 0.10 mg/mL for brucine (sR = 1.0%). The stability of mixture solution were better under the conditions of methanol solution at 4 ℃ or -20 ℃. Conclusion The results provide a possible way to develop the mixture solution in place of the monomer reference, and the mixture reference solution is expected for the quality control in the slices of Semen Strychni and its compound preparations.
9.Genetic characterization of three CRF01_AE full-length HIV type 1 sequences from Fujian Province, China.
Hai-long HUANG ; Ping-ping YAN ; Jian ZHENG ; Shou-li WU ; Ge CHENG ; Xun LIN ; Wu-xiong ZHENG ; Mei-rong XIE ; Jian-ming ZHANG ; Yan-sheng YAN
Chinese Medical Journal 2006;119(19):1622-1628
BACKGROUNDOne of the major characteristics of the human immunodeficiency virus type 1 (HIV-1) is its unusually high degree of genetic variability, which involves in genetic diagnosis, subtyping, vaccine design, and epidemiology. HIV-1 CRF01_AE is a main prevalent HIV-1 recombinant strain in China. In this study, three full-length CRF01_AE genomes from Fujian Province, China were cloned, sequenced, and analyzed; and the further genetic diversity defining and epidemiologic analysis were carried out.
METHODSProviral DNA was extracted from non-cultured peripheral blood mononuclear cells, the near full-length HIV-1 genome was amplified and the PCR products were cloned into pCR-XL-TOPO vector and sequenced. 5'-long terminal repeat (LTR) and 3'-LTRs were amplified by additional independent PCR and cloned into pMD18T vector. Gene-based phylogenic tree was constructed and genetic distances were calculated by MEGA 3.1. Simplot was used for Bootscan analysis.
RESULTSThe phylogeny and genetic distance analysis of the three near full-length sequences confirmed that these three samples clustered with CRF01_AE isolates, more close to Thailand CRF01_AE strain CM240, and were distantly related to African CRF01_AE strain 90CF402. Analysis of their genomic organization revealed the presence of nine potential open reading frames. There were no major deletions, rearrangements, or insertions in the three sequences, but an in-frame stop codon was found in tat gene of Fj051. LTRs of the three sequences contained a few nucleotides mutation. We did not find new mosaic recombinant in the three sequences. The V3 motif was GPGQ in all the three sequences, and there were only few amino acids differences in all three V3 loop sequences.
CONCLUSIONThis report reveals the background of the three full-length CRF01_AE genomes, the most dominantly circulating HIV-1 strain in Fujian Province, China. The work is essential for the design and development of an effective AIDS vaccine for the region.
Adult ; Amino Acid Sequence ; Base Sequence ; DNA, Viral ; chemistry ; Female ; Genome, Viral ; HIV Long Terminal Repeat ; HIV-1 ; classification ; genetics ; Humans ; Male ; Molecular Sequence Data ; Phylogeny ; Recombination, Genetic
10.Radiotherapy of unicentric mediastinal Castleman's disease.
Yue-Min LI ; Peng-Hui LIU ; Yu-Hai ZHANG ; Huo-Sheng XIA ; Liang-Liang LI ; Yi-Mei QU ; Yong WU ; Shou-Yun HAN ; Guo-Qing LIAO ; Yong-Dong PU
Chinese Journal of Cancer 2011;30(5):351-356
Castleman's disease is a slowly progressive and rare lymphoproliferative disorder. Here, we report a 55-year-old woman with superior mediastinal Castleman's disease being misdiagnosed for a long term. We found a 4.3 cm mass localized in the superior mediastinum accompanied with severe clinical symptoms. The patient underwent an exploratory laparotomy, but the mass failed to be totally excised. Pathologic examination revealed a mediastinal mass of Castleman's disease. After radiotherapy of 30 Gy by 15 fractions, the patient no longer presented previous symptoms. At 3 months after radiotherapy of 60 Gy by 30 fractions, Computed tomography of the chest showed significantly smaller mass, indicating partial remission. Upon a 10-month follow-up, the patient was alive and free of symptoms.
Antigens, CD20
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metabolism
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Castleman Disease
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diagnosis
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immunology
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pathology
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radiotherapy
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surgery
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Female
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Follow-Up Studies
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Humans
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Mediastinal Diseases
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diagnosis
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immunology
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pathology
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radiotherapy
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surgery
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Mediastinum
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diagnostic imaging
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pathology
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Middle Aged
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Multimodal Imaging
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Positron-Emission Tomography
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Radiotherapy, Intensity-Modulated
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Tomography, X-Ray Computed