Objective]The article summarizes the essentials of clinical dialectical experience by professor Zhang Qin on threatened abortion. [Methods]From following professor Zhang Qin clinical studies, studying professor Zhang Qin’s medical cases and selecting medical records on uterine hemorrhage with placenta low set, recurrent miscarriage with prothrombotic state, serious uterine hemorrhage with repeated vaginal bleeding, we can summarize the thoughts and experience on treating threatened abortion.[Results]Professor Zhang Qin considers that threatened abortion occurs mainly because of the deficiency of kidney ,maybe because of the deficiency of qi failing to lift or the deficiency of yin or the blood heat accompanied with blood stasis which leading to hemorrhage. We can respectively treat them by nourishing and lifting qi, nourishing yin or by clearing away heat to stanch the flow.[Conclusion] Professor Zhang Qin’s experience on treating threatened abortion has positive effect and is worth of further study.

BACKGROUND:Inflammation is an important factor in cervical spondylotic vertebral arteriopathy, bone marrow mesenchymal stem cells have the potential to treat cervical spondylotic vertebral arteriopathy because of the immunomodulatory effects to inhibit inflammation.
OBJECTIVE:To investigate the injury mechanism of vascular injury in the model of cervical spondylotic vertebral arteriopathy and to study the therapeutic effects of bone marrow mesenchymal stem cells on it.
METHODS:Forty Japanese big ear rabbits were randomly divided into four groups:control group, model group, tanshinone group, and stem cellgroup. After modeling, the control and model groups were not given intervention, while the tanshinone and stem cellgroups were injected with tanshinone II A sodium sulfonate solution (10 mL) and bone marrow mesenchymal stem cellsuspension (10 mL) along the ear vein, respectively. After 2 weeks, the routine pathological examination was done to observe the vascular morphological changes, immunofluorescence staining was done to observe the cathepsin B expression in the vertebral artery, and ELISA was used to detect the expression of tumor necrosis factor-αand interleukin-6 in the vertebral artery.
RESULTS AND CONCLUSION:Compared with the model group, the arterial smooth muscle cellhypertrophy and hyperplasia was obviously restrained in stem cellgroup, and vascular endothelial fold was in symmetry, while no significant difference was found between stem cellgroup and tanshinone group. Compared with the model group, expressions of cathepsin B, interleukin 6 and tumor necrosis factor-αexpression were reduced significantly in the stem cellgroup (P<0.05), while there was no difference between the model and tanshinone groups (P>0.05). Inflammatory reaction may be one of mechanisms for vertebral artery damage, and bone marrow mesenchymal stem cells can effectively inhibit inflammation of the vertebral artery and improve vascular remodeling.

ObjectiveTo observe the effects of basic fibroblast growth factor (bFGF) on growth period of cultured bovine retinal pigment epithelium cells in different durations.MethodsDifferent proportion of phase with 4 ng/ml bFGF were measured by Flow Cytometry (FCM) assay.ResultsbFGF increased the RPE cells' proportion of phase S and decreased the proportion of phase G0-G1. ConclusionbFGF can stimulate RPE cells from phase G1 to phase S.

Adult ; Epithelioid Cells ; pathology ; Humans ; Male ; Perivascular Epithelioid Cell Neoplasms ; pathology

Cystadenocarcinoma, Serous ; diagnosis ; pathology ; Female ; Humans ; Ovary ; pathology

Objective To observe the effect of low molecular weight heparin(LMWH) in treatment of diabetic foot.Methods Fifty-four patients were divided into test group and control group randomly.Both two groups were given routine therapy including mediating blood glucose with Insulin,anti-infection and changing dressings,while the test group were givon LMWH 5000IU once datly every 12 hours for 15 days besides the routine treatment.Then the endothelin(ET),thromboxaneB2(TXB2),hemorheology,ankle-brachial index(ABI),ulcer healing,fibrinogen(FIB),prothrombin time(PT) and avtivated parital thromboplastin time(APTT) were observed.Results Compared to that of before treatment,there are ET,TXB2,hemorheology and better ulcer heals after treatment,The improvement in test group patients was superior to that of control group(P0.05) after treatment in two groups.No hemorrhage and other complications occurred.Conclusion LMWH is effective and safe for treatment of diabetic foot.

China ; epidemiology ; Humans ; Occupational Diseases ; epidemiology ; prevention & control

Objective To assess the effect of sodium nitroprusside (SNP)-induced hypotension on gastric intramucosal pH (pHi), gastric intramucosal PCO2 ( PgCO2 ) and the difference between PgCO2 and PaCO2 (PgCO2-PaCO2) .Methods Fifteen ASA I patients (11 male, 4 female) aged 22-43 yr8, weighing 51-68 kg undergoing laminectomy for decompression of spinal cord in prone position were enrolled in this study. The patients were intubated and mechanically ventilated with 100% O2 after induction of general anesthesia. Anesthesia was maintained with isoflurane (1.2-1.4%) . Radial artery was cannulated for BP monitoring and boood sampling. TRIP tonometry catheter 14 F was inserted into stomach and connected to Tonocap ( Datex-Ohmeda, Finland) . Controlled hypotension was induced with SNP infusion at 1-5 ?g kg-1 min-1 . MAP was reduced to (60 ?5) mm Hg in 5-7 min and maintained for 60 min. Blood samples were taken before induced hypotension (T, ) , at 30 min (T2 ) and 60 min of hypotension (T3) and 30 min after hypotension (T4) for blood gas analysis. pHi, PgCO2 -PaCO2 and PETCO2 were also determined.Results MAP decreased by 35% and 33% at T2 and T3 as compared with the baseline (T, ) . pHi decreased significantly and PgCO2, PgCO2-PaCO2 increased significantly at T, as compared with those at T( ( P 0.05). pH at T2 ,T3 and T4 and BE at T3 and T4 were significantly lower than the baseline value (T1). PaCO2 and PET CO2 were significantly higher at T4 than those at T, (P

OBJECTIVETo study the expression difference in MicroRNA-21 (miRNA-21) levels of the plasma between the patients with coronary heart disease and the subjects without coronary artery lesions, and its clinical significance.

METHODSPlasma was obtained from the patients with coronary heart disease(trial group,56 cases) and the subjects without coronary artery lesions(control group, 10 cases), patients with coronary disease were divided into angina(AP, 39 cases) and acute myocardial infarction(AMI, 17 cases)subgroup, the contents of miRNA-21 were detected using qRT-PCR method, and the differential expression of miRNA-21 in each group was analyzed. The levels of creatine kinase isoenzyme (CK-MB), high sensitive troponin I(cTnI), B type natriuretic peptide urea (BNP), Gensini, left ventricular ejection fraction (LVEF), integral value of coronary left ventricular end diastole diameter (LV) and homocysteine (HCY) were determined and the correlation between miRNA-21 and these clinical indexes was analyzed.

RESULTSCompared with control group, there was a significant difference in expression of miRNA-21 in patients with angina and AMI (P < 0.05), and miRNA-21 expression in AMI group was much higher than that in AP group. There was statistical significance in CK, CK-MB, cTnI, Genisis integral comparison between AMI group and control group (P < 0.05). The correlation analysis showed that there was a positive correlation between expression of CK, CK-MB, cTnI and the level of circulating miRNA-21 in patients with acute myocardial infarction. And there was a negative correlation between BNP, Gensini integral, LVEF value of coronary artery, LV and circulating miRNA-21.

CONCLUSIONmiRNA-21 was significantly elevated in acute myocardial infarction subgroup than the control group. The level of miRNA-21 associates with the degree of coronary artery stenosis, and might be a potential marker for the diagnosis of acute myocardial infarction. miRNA-21 may play an important role in protecting myocardium from ischemia/reperfusion injury.

Acute Disease ; Biomarkers ; blood ; Coronary Disease ; blood ; Creatine Kinase, MB Form ; metabolism ; Humans ; MicroRNAs ; blood ; Myocardial Infarction ; blood ; Natriuretic Peptide, Brain ; metabolism ; Troponin I ; metabolism

Adenocarcinoma, Mucinous ; diagnosis ; pathology ; Cystadenocarcinoma, Mucinous ; diagnosis ; pathology ; Diagnosis, Differential ; Female ; Humans ; Ovarian Neoplasms ; pathology ; Ovary ; pathology