OBJECTIVETo investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line.

METHODSColumn chromatography, thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS).

RESULTSThe results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 μmol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, Artemisinin, in a dose-dependent manner, could significantly increase the level of ROS.

CONCLUSIONArtemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.

Annexin A5 ; metabolism ; Apoptosis ; drug effects ; Artemisinins ; pharmacology ; Caspase 3 ; metabolism ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Colorimetry ; Flow Cytometry ; Humans ; Iron ; pharmacology ; Pancreatic Neoplasms ; enzymology ; pathology ; Propidium ; metabolism ; Proton Magnetic Resonance Spectroscopy ; Reactive Oxygen Species ; metabolism ; Time Factors ; fas Receptor ; metabolism