Objective To identify biomarkers associated with the differentiated phenotype based on gene expression profiling of gastric cancer. Methods Two bioinformatic methods, BAGEL and k-TSP, were used to identify featured genes associated with differentiation in gastric cancer samples based on the Oligo gene chip data, and ROC curves were used to verify the classification sensitivity and specificity of the identified genes. Finally, a total of 30 gastric cancer samples with different differentiation levels were collected for laboratory validation using real-time PCR analyses. Results A total of 121 differentially expressed genes were identified using the BAGEL algorithm, the criterion were FC ＞ 2. 0 and P ＜ 0. 001.Then, the k-TSP algorithm for feature selection based on this differential expression data were used, and 3 groups of featured genes which had potential to classify poor and well differentiation gastric cancer samples were identified, including MYLIP and TMPRSS3, ZNF266 and TM4SF1, SNAI2 and CNFN. To define the featured gene groups that had the highest classification capability, ROC curves to calculate the classification sensitivity and specificity of each gene group were used. The results showed that the combination of SNAI2and CNFN as a classifier had the highest classification sensitivity and specificity. Real-time PCR results showed that 18 of 22 poor differentiation samples were found with high expression of SNAI2 and low expression of CNFN (82%); 6 of 8 well differentiation samples were of low expression of SNAI2 and high expression of CNFN (75%). Conclusion The results indicate that SNAI2 and CNFN are constantly expressed in poor or well differentiation gastric cancer samples, and the expression pattern of these two genes is opposite. These results indicate that SNAI2 and CNFN have the potential for the identification of the differentiation level of gastric cancer.

Objective To explore the immunoregulatory effect of Oldenlandia ( Hedyotis diffusa Villd.) , by investigating on the expression of macrophage cytokines and on TLR-4 inflammatory signal transduction pathway , aiming to propose a new experimental and theoretical evidence for the use of oldenlandia . Methods Three common cytokines , such as IL-6 , MCP-1 and Cxcl1 in macrophage cells were detected by using RT-PCR after treatment with oldenlandia solution .Autodock software was used to analyze the correlation between the main components and TLR-4 so as to explore the mechanism of expression chan-ges of the cytokines.Results Oldenlandia solution could upregulate the expression of Cxcl 1, which might depend on the combination of TLR 4 and stigmasterol-beta-D-glucoside , trans-4-methoxycinnamyl alcohol , and asperulosidic acid .Conclusion Oldenlandia could activate TLR-4 signal pathway and thus affect the expression of Cxcl 1 and function of macrophage .It can be inferred that oldenlandia could regu-late immunity , which could serve as the theoretical rationale for the development of new drugs in the labo-ratory.

Gastric cancer (GC) is one of the most frequent malignant tumors. In order to systematically characterize the cellular and molecular mechanisms of intestinal GC development, in this study, we used 22 K oligonucleotide microarrays and bioinformatics analysis to evaluate the gene expression profiles of GC in 45 tissue samples, including 20 intestinal GC tissue samples,20 normal appearing tissues (NATs) adjacent to tumors and 5 noncancerous gastric mucosa tissue samples. These profiles allowed us to explore the transcriptional characteristics of GC and determine the change patterns in gene expression that may be of clinical significance. 1519 and 1255 differentially- expressed genes (DEGs) were identified in intestinal GC tissues and NATs, respectively, as determined by Bayesian analysis (P < 0.001). These genes were associated with diverse functions such as mucosa secretion, metabolism, proliferation, signaling and development, which occur at different stages of GC development.