1.Preliminary analysis on the gene expression profiles of medulloblastomas by use of cDNA array.
Rong-cai JIANG ; Pei-yu PU ; Chang-hong SHEN ; Shiz-hu YU ; Bao-hua JIAO ; Hu WANG ; Chun-yan WANG ; Chun-sheng KANG
Chinese Journal of Medical Genetics 2004;21(2):179-181
OBJECTIVETo explore the molecular genesis of medulloblastomas with cDNA array.
METHODSFour samples of medulloblastomas and 1 sample of normal brain tissue were collected freshly. After total RNA extraction, the (32)P targeted cDNA probes were converted and then hybridized with Atlas Human Cancer Array 1.2. The gene expression profiles were acquired through autoradiography. The discrepancy between the tumor and the normal brain tissue was analyzed with Atlas Image 1.01a.
RESULTSIn comparison with the genes in the normal brain tissue, 6 down-regulated and 35 up-regulated genes in the medulloblastomas were revealed by means of the microarrays and autoradiography, and were verified by reverse transcriptase-PCR. The regulatory trends of most differential expression genes were in compliance with the biological features of this tumor.
CONCLUSIONMedulloblastomas are diseases involving multiple genes with some molecular pathological mechanisms different from the astrocytic gliomas. There are complex interrelationships between these genes, which need to be further researched.
Child ; Child, Preschool ; Gene Expression Profiling ; Humans ; Medulloblastoma ; genetics ; Oligonucleotide Array Sequence Analysis
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