Objective To evaluate antitumor effect of adenovirus-mediated cytosine deaminase (CD) gene and 5-fluorocytosine (5-FC) on rectal cancer cells.MethodsCD gene was transinfected into HR-8348 rectal cacer cell lines with recombinant adenovirus mediation. The transinfection rate and expression of CD gene were detected. Plating efficiency and MTT method were used to examine inhibition of HR-8348 cell growth, and antitumor effect of CD/5-FC were evaluated on HR-8348 experimental tumor in nude mice.ResultsHigh transinfection effeciency and expression of CD gene were achieved in HR-8348 with adenovirus mediation. CD/5-FC system showed a remarkble effect of inhibition on plating efficiency and growth of the tumor cells transinfected with CD gene, but no act on tumor cells without CD gene transinfection. In a mixture of HR-8348 tumor cells with and without CD gene transinfection, CD/5-FC killed both types of tumor cells and revealed a powerful "by-stander effect". With nude mice experiment, 71.5% of inhibition of solid tumoral xenografts of HR-8348 cells was found.ConclutionCD/5-FC has significant antitumor effect on rectal cancer cells with adenovirus-mediated CD gene transinfection and has also "by-stander effect" on tumor cells.

Objective To study the expression of cathepsin B (CatB) and metallothionein (MT) in colorectal cancer tissue, and explore their association with lymphatic and hepatic metastasis. Methods Immunohistochemistry technique was used to assay the expression of CatB and MT in 82 cases of primary colorectal cancer, normal colon mucosa, and lymph node and hepatic metastasis. The relationship between the expressions of CatB and MT and the clinical stage or pathological grading of the colorectal cancer were analyzed. Results The rate of CatB expression in primary tumor, normal colon mucosa, and lymph node with metastasis and hepatic metastasis was 48.8%, 20.7%, 66.7%, and 59.1%, respectively, and that of MT expression was 53.7%, 26.8%, 71.4% and 72.7%, respectively. The rates of CatB and MT expression in primary tumor, and lymph node and hepatic metastasis were higher than that in normal mucosa tissue. The rate of coexpression of CatB and MT in lymph node and hepatic metastasis was higher than that in primary tumor and lymph node without metastasis. As regard to clinical stages, the positive rates of CatB and MT in Dukes C and D were higher than that in Dukes A and B (?2=11.024 4, 10.933 8, P

Objective To explore the influence of cathepsin B (CatB) expression on the ability of adhesion and invasiveness of colorectal cancer cells. Methods Human colon cancer cell line LoVo and rectal cancer cell line HR-8348 were used in the present study. The total length of CatB mRNA was cloned and transfected into the cells, and the CatB expression was detected by RT-PCR. Cell adhesion was detected with Matrigel and invasiveness was assessed with transwell method. Results CatB mRNA expression was detected and enhanced expression was found in LoVo and HR8348 cells tranfected with pcDNA3CatB. Cell adhesion rates to Matrigel stromatin in control LoVo cells, empty vector transfected cells and CatB transfeted cells were 0.461 6?0.148 7, 0.412 1?0.215 8 and 0.691 6?0.150 8, respectively. The adhesive rate of CatB transfected LoVo cells was higher than that of the control cells and the empty vector transfected cells with significant differences (F=5.839 0, P

Objective To study the differentially expressed proteins and their biological behavior in colorectal carcinoma tissues and the normal colonic mucosa by proteomics and molecular biology techniques. Methods The technique of fluorescence two dimension differential gel electrophoresis (2-D DIGE) was used to analyze the expression of differential proteins in normal colorectal mucosa and primary cancer foci. Liquid chromatography with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was used to identify the differential proteins. Transfection experiment of colorectal cancer cells was performed with the differential protein cDNA, and the changes in cytobiological behavior were observed. Results Significant differences of protein expression levels were found by two-dimension electrophoresis. Eight differential protein spots were analyzed and identified. Human carbonic anhydrase Ⅱ and protein disulfide isomerase were detected in normal colorectal mucosa, but not in primary cancer foci. Phosphoglycerate kinase 1, fumarate hydratase and aldolase A were expressed in primary cancer. After transfection with human carbonic anhydrase Ⅱ cDNA, the abilities of Lovo cells were obviously reduced in invasiveness, chemotaxy motor and drug resistance. Conclusions Differences on protein expression levels are found between normal colorectal mucosa and primary cancer foci by 2-DE DIGE. The pathogenesis of colorectal carcinoma is related to the reduced expressions of carbonic anhydrase II and protein disulfide isomerase and enhanced expression of aldolase A. The technique of differential proteomics is useful in reaching a indepth understanding of the pathogenesis mechanisms of human colorectal cancer.

Objective To investigate the level of coronary endothelial injury and dysfunction, the jeopardy score of coronary artery lesion by angiography, and their correlations in patients with stable angina (SA) or unstable angina (UA). Methods The 20 patients with UA, 17 patients with SA and 18 patients excluding coronary heart disease (control) were studied. Blood samples were collected during percutaneous coronary intervention (PCI). To investigate the coronary endothelial injury and dysfunction, we measured the concentrations of nitric oxide (NO)? endothelin (ET) and the number of circulating endothelial cells (CEC) in coronary sinus blood. The jeopardy score for coronary artery lesion was evaluated according to the method by Califf. Results In angina patients, especially in UA patients, NO concentration in coronary sinus blood was lower, while ET concentration and CEC number were markedly higher than those in control group. The jeopardy scores showed no difference between UA and SA. The coronary endothelial injury and dysfunction showed a positive correlation with jeopardy scores. Conclusion The coronary endothelial injury and dysfunction is not only the initiation factor, but may play a key role in the progression of atherosclerosis and coronary heart disease. Coronary angiography combined with detection of coronary endothelial injury and dysfunction may better describe the lesion of coronary artery, and evaluate the prognosis of patients with coronary heart disease.

Objective To evaluate the target killing effect and metastasis prevention effect of soluble Fas combined with PKC inhibitor on the growth of human colorectal carcinoma implant in nude mice. Methods Orthotopic implantation and metastasis model of human colorectal carcinoma was reproduced in nude mice. Tumor tissue of tumor cell line HR-8348 with positive expression of FasL was implanted to the colonic wall of nude mice. After one week of tumor growth, mice were randomly divided into four groups according to the different agents injected into the peritoneal cavity. Twelve mice were in each group. The mice in the combined treatment group (recombinant soluble Fas coupled with PKC inhibitor + 5-Fu) were injected intraperitoneally 100?l (3mg/ml) recombinant soluble Fas coupled with PKC inhibitor and 0.5 mg of 5-Fu. (On the day of 0, 4, 8, 12 and16). At the same time, a group of tumor bearing mice were given recombinant soluble Fas coupled with PKC inhibitor only, and another group with 5-Fu only, and in the control group only normal saline was given. One month after implantation, tumor weight, inhibition rate and the presence of metastasis were evaluated respectively after the mice were sacrificed. Results Compared with control group, the orthotopically implanted tumors were significantly reduced in weight in mice treated with 5-Fu, recombinant soluble Fas coupled with the PKC inhibitor, and combined treatment, with respective inhibited rates of 43.1%, 79.9%, and 86.3%. Liver metastasis was also inhibited with significant decrease in incidence in 5-Fu group, recombinant soluble Fas coupled with the PKC inhibitor, and combined group compared with that in control group (75.0% vs 36.4%, 16.7%, and 0%). The incidence of peritoneal metastasis was also decreased significantly in 5-Fu, recombinant soluble Fas coupled with PKC inhibitor, and combined treatment compared with that in control group (100% vs 45.5%, 16.7%, and 8.3%, P

Objective To compare the clinical pathological characteristics in young and old patients with colorectal cancer (CRC) and investigate their relationship with prognosis. Methods A retrospective review was made in 68 CRC patients less than 35 years old and 322 CRC patients older than 65 treated in our hospital from July 1993 to July 2003. Their clinical manifestation, pathological feature, Dukes staging, misdiagnosis rate and results of following-up were compared. Results The main manifestation in young group was abdominal pain (69.1%), but in old group was hemafecia or mucous bloody stools (53.7%). The ratio of poorly differentiated neoplasm was obviously higher in young group (48.5%) than in old group (20.5%) (P

Objective To assess examinational methods of Multi-slice CT(MSCT)in diagnosis of palatine lesions. Methods 28 cases were performed MSCT examination with the conventional method,extending -tongue method,colliding-tongue method and pronouncing method. All images were reconstructed by means of multiplana reconstruction (MPR) and CT virtual endoscope (CTVE). Results Correct diagnosis were obtained through the complementarity of the methods. Conclusion Reasonable examination methods of MSCT is apt to display palatine lesions.

Objective To investigate the quality assurance(QA) and quality control(QC)of 16 slices helical CT angiography in the lower limb. Methods 42 cases clinically suspected as the arterial disease of lower limb undergoing MSCTA were analyzed retrospectively. All reconstructed images were reformed by means of MIP, VR, MPR and CTVE. Results 22 patients were diagnosed correctly by using MSCTA. Compared to DSA and/or surgical results. Sensitivity, specificity and accurate rate were all 100%. Conclusion It is important to strengthen the QA and QC of the 16 slices helical CT angiography in the lower limb.

Objective To investigate the molecular epidemiology of CRAB isolated from children in wuhan. Methods Forty non-repetitive strains of CRAB were collected from hospitalized children of emergency department, neonatal medicine, cardiothoracic surgery, bone surgery, respiratory medicine and renal medicine in Wuhan children's hospital during December 2008 and May 2009. MIC values were PFGE; KPC, IMP, GIM, SPM, SIM, OXA-23, VIM genes and integrase gene were amplified by PCR and then sequenced to confirm the genotypes.; Plasmid conjugation experiment was used to study the transfer method of bacterial resistance and southern blot hybridization was used to target the resistance genes. Results Susceptible rates of 40 strains to gentamicin, tobramycin, amikacin, ciprofloxacin, levofloxacin, trimoxazole were 20%, 5%, 93%, 93%, 95%, and 23% respectively. Eleven types of clone were detected by PFGE,including 29 strains of type A clone, 2 strains of type B clone, and 1 strain for each type of C to K clone. Eleven isolates produced both IMP-4 and OXA-23 carbapemase. Twenty-six isolates only possessed OXA-23 carbapemase. Thirty-six strains carried class Ⅰ integron. The results of southern blot hybridization showed that Intl, IMP-4 and OXA-23 type were located on chromosome. Conclusions Type A clone of CRAB is the most common. OXA-23 and IMP-4 type are the major acquired carbapemases, especially the OXA-23 is the most common type. The horizontal transmission of OXA-23 and IMP-4 gene mediated by Int1 and the spread of type A resistant clone is the major way of the spread of carbapenem-resistant Acinetobacter baumannii in the region.