Objective To observe the effects of solanine on DNA and RNA level in tumor cell of S_(180) and H_(22) tumor-bearing mice.Methods S_(180) and H_(22) mice were divided into solanine(37.50、18.75,and(9.37) mg/kg) groups,negative control group and cytoxan(30 mg/kg) group,whom were given drugs by sc.Levels of RNA and DNA in tumor cell membrane in every groups were examined,respectively by laser scanning confocal microtechnic technology.Results Solanine(37.50、18.75 mg/kg) groups reduced the ratio of RNA and DNA in tumor cell of both S_(180) and H_(22) mice.Conclusion Solanine can reduce the ratio of RNA and DNA in tumor cell of S_(180) and H_(22) mice,which may be one of the mechanisms of solanine's(antitumor) effect.

Objective:To investigate the characteristics and regularities of adverse drug reactions(ADRs) induced by quionlones antibacterial agents in our hospital.Methods:187 ADR cases induced by quionlones collected by the ADR drugs monitoring center in our hospital from 2007～2008 were classified and analyzed.Results:187 ADR cases were involved in 6 drugs of quionlones.The ADRs were chiefly manifested as lesions of skin and its accessories,and followed by the reaction of the digestive system.Most ADRs induced by the intravenous route had a good recovery.Quionlones causing most ADRs was gatifloxacin.Conclusion:The occurrence of ADRs caused by quionlones was related to many factors. Great importance should be attached to ADR monitoring so as to reduce the incidence of ADRs by quionlones

Objective To studied the effects of administering a second rinse solution of low-potassium-dextran(LPD) before reperfusion on the donor lung of rat after long time,cold ischemic preservation.Methods Twenty-four rats were randomly divided into control group(n=12) and trial group(n=12).Donor lungs of control group were flushed with LPD solution.The levels of malonaldehyde(MDA),myeloperoxidase(MPO) and interleukin-8(IL-8) were tested in the tissue of right lung and the left lungs were reperfused with venous blood for 30 minutes.The partial pressure of oxygen(PaO_2),PaP and PawP were measured at every 10 minutes intervals during reperfusion.After reperfusion,the MDA,MPO activity and interlukin-8(IL-8) were determined in the lung tissue.Results The levels of MDA,MPO and IL-8 decreased significantly in right lung of trial group than those in control group(P

Objective To explore the mechanism of gene Nelin on the differentiation of vascular smooth muscle cells(VSMC).Methods Human VSMCs were stably infected with over-express lentiviral vector [Nelin-VSMC] and interference lentiviral vector [LV-Nelin-SiRNA-VSMC] by Western blotting and RT-PCR.The effect of Nelin expression on phenotypic transition of VSMCs was observed and the mechanism involved was explored.Results The Nelin-VSMC cells take on the contractile phenotype of long slender cells possessing hill-valley characteristics.Overexpression of Nelin can enhance SMoα-actin expression with the increase of total RhoA and nuclear serum response factor(SRF) expression.Treated with a selective Rho kinase inhibitor Y-27632,the expression of SMα-actin and nuclear SRF protein induced by Nelin was effectively reduced.Meanwhile,the opposite results were observed in the Nelin-depression VSMC.Conclusions Nelin can enhance the expression of SMα-actin and carry a pivotal role in the process of VSMCs differentiation and phenotype modulation by activating Rho and SRF.

BACKGROUND:For on-visit root canal therapy, ultrasound washing can improve the quality of root canal irrigation, and play a synergistic role with ultrasound so that the flushing fluid can reach the area that cannot be completely cleaned up by root canal preparation instruments, such as apical isthmus and col ateral root canal, for removal of the detritus, cleanup and disinfection. ＜br> OBJECTIVE:To evaluate the clinical effect of one-visit root treatment with nickel-titanium rotary instruments and ultrasonic irrigation unit in treatment of chronic apical periodontitis. ＜br> METHODS:A total of 460 patients with il ed 460 teeth were selected. Patients were randomly divided into three groups:Mtwo instrument and ultrasonic technique combined with sodium hypochlorite was utilized in group A;ultrasonic technique combined with active silver ion antibacterial solution was utilized in group B;stainless steel K files with saline and hydrogen peroxide solution were used as irrigations in group C. Twenty-four hours after root canal fil ing, the pain degree was self-assessed by patients with visual analogue scale, and subsequent visit was done 7 days after root canal fil ing. Six and twelve months after root canal fil ing, the therapeutic effect was determined with X-ray examination and clinical assessment. ＜br> RESULTS AND CONCLUSION:The degree of pain and occurrence rate of acute reactions in groups A and B were significantly lower than those in group C (P＜0.05), but there was no significant difference between groups A and B (P>0.05). Six months after root canal fil ing, the cure rate was not significantly different between three groups (P>0.05). But after 12 months, the cure rate in the group C was significantly lower than that in groups A and B (P＜0.05). By using of nickel-titanium rotary instruments and ultrasonic irrigation unit in treatment of chronic apical periodontitis, the short-and long-term effects of one-visit root treatment have been achieved clinical y.

Objective To establish the quality standard for Shengxinfa Capsule. Methods TLC was used to identify Praeparata, Psoraleae Fructus and Gastrodiae Rhizoma in Shengxinfa Capsule, and 2,3,5,4’-the four-stilbene-2-O-β-D-glucoside was identified by HPLC. The column of Agilent TC-C18 (4.6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-water (15∶85). The flow was 1.0 mL/min and column temperature was 30 ℃. UV detecting wavelength was 320 nm. Results Praeparata, Psoraleae Fructus and Gastrodiae Rhizoma could be identified by TLC. The linear range of 2,3,5,4’-the four-stilbene-2-O-β-D-glucoside was in the range of 0.05-0.50 μg. The average recovery was 97.8% (RSD=0.39%). Conclusion The method is feasible and accurate. The quality of Shengxinfa Capsule can be controlled effectively with the established quality standard.

Objective To determine the content of taurine in Mactra veneriformis by pre-column derivation HPLC.Methods Ultrasound method was used for the extraction of taurine from Mactra veneriformis.In alkaline condition,amino acids reacted with phenyl isothiocyanate and resulted in the derivatives of benzene isothiocyanate-amino acid complex.Kromasil C18 column(4.6 mm ?250 mm,5 ?m) was used and the absorbance at 254 nm was measured.The mobile phase consisted of sodium acetate solution,water and methanol.Results Corresponding chromatographic peaks of taurine were obtained in extract sample and the control.The linear range of taurine was from 10.04 ?g?mL-1 to 50.20 ?g?mL-1,and the recovery was 97.95 %(RSD=1.41%,n=9).Conclusion This method is simple,accurate and reproducible.The seasonal variation range of taurine level is from 10.56 mg/g to 20.91 mg/g,and the taurine level arrived to the peak in June.

Objective The fingerprint of volatile components from three breeds of Radix Curcumae was compared to supply evidence for the standard of quality control of Radix Curcumae.Methods Gas chromatography was used to establish fingerprint of Radix Curcumae and GC-MS chromatography was applied to identify its characteristic peaks. The similarity of fingerprint was evaluated by computing method.Results The fingerprint had its similarity and characteristics in different breeds of Radix Curcumae. The fingerprint detection is a good method for identifying Radix Curcumae,RSD 2.53 %.Conclusion This method is simple and effective for identifying different breeds of Radix Curcumae and can be used to control the quality of medicinal material of Radix Curcumae.

OBJECTIVE:To probe into the characteristics and regularity of adverse drug reactions(ADR)cases occurred in our hospital.METHODS:535 ADR cases reported in our hospital in 2008 were collected and analyzed statistically.RESULTS:In the incidence of 535 ADR cases,the ratio of male to female were 1.10∶1.Patients over the age of 60 years showed the highest incidence,accounted for 36.82%(n=197).430 ADR cases were induced by intravenous route(80.37%);254 cases were induced by antimicrobials(47.48%),followed by TCM preparations(n=75,14.02%).The ADR were chiefly manifested as lesion of skin and its appendants in 212 cases(39.63%),followed by 164 cases of lesion of digestive system(30.65%).Most ADR cases had a favorable prognosis.CONCLUSION:It should be strengthened to offer education and guidance of ADR monitoring for medical staff so as to make sure the safety,effectiveness and convenience of drug use in the clinic.

Background Central retinal vein occlusion (CRVO) is a common retinal vascular disease.Intravitreal injection of ranibizumab, a vascular endothelial growth factor (VEGF) antibody, is being a useful approach to the treatment of macular edema secondary to CRVO.However,little literature about choroidal thickness variations following intravitreal injection of ranibizumab for CRVO is published up to now.Objective This study was to observe the dynamic changes of macular choroidal thickness after intravitreal injection of ranibizumab in CRVO eyes.Methods A self-controlled series cases study was designed.Thirty-one eyes of 31 CRVO patients were included in Tianjin Eye Hospital from June 2013 to November 2014,with the males 19 and females 12 and mean age of (51.13±16.65) years.Ranibizumab (5 mg,5 ml) was intravitreally injected in the CRVO eyes once per month for 3 times by the same operator.A enhanced depth image (EDI) mode of spectral-domain OCT system was employed to measure the choroidal thickness at subfoveal, 1 mm from fovea nasal and 1 mm from fovea temporal before and 1 month,2,3 months after first injection in both CRVO eyes and contralateral healthy eyes, respectively.The best LogMAR vision was recored.This research protocol was approved by the Ethic Committee of this hospital, and written informed consent was obtained from each individual prior to any medical examination.Results Retinal bleeding was exhibited in the CRVO eyes in color photography,and fundus fluorescein angiography showed the fluorescine leakege in the early phase and fluorescine accummulation in the late phase.The mean choroidal theckness value was (325.32±83.04) , (294.83±80.61), (315.95±90.77) and (314.81±84.98) μm before injection and 1,2,3 months after injection,respectively,showing a significantly difference among various time points (F =7.96,P =0.00) , and the choroidal theckness values were evidently reduced in various time points after injection in comparison with before injection (P =0.01,0.01,0.00).The choroidal thickness value at foveal was (314.81±84.98) μm in the CRVO eyes 3 months after injection,and that in the fellow eyes was (260.47±55.90) ,with significant difference between them (t =2.95, P =0.01).The LogMAR vision was 0.17±0.09,0.37±0.23,0.42±0.26 and 0.49±0.21 before and 1,2,3 months after injection,with the significant difference among various time points (F =21.50, P =0.00) and showed considerable improvement after intravitreal injection of ranibizumab(all at P＜0.01).The mean retinal thickness value was (244.14-±23.28) μm in the fellow eyes, and those in 1 month, 2,3 months after injection were (523.81 ± 147.61), (352.13 ± 166.71),(376.39±209.46) and (369.00±225.61) μm in the CRVO eyes, showing obvious reduce after intravitreal injection, with significant difference among different time points (F =7.09, P＜0.01).Conclusions Choroidal thickness at macular fovea is obviously increased in CRVO eyes compared with the contralateral healthy eyes.Intravitreal injection of ranibizumab can reduce choroidal thickness and therefore improve vision.EDI OCT is available in the evaluation of dynamic change of choroidal thickness.Macular choroidal thickness could be used as a predictor of CRVO prognosis following intravitreal ranibizumab.