Gemcitabine has consistently shown activity as a new agent in non-small-cell lung cancer. In non-small-cell lung cancer, gemcitabine-cisplatin combination response rates range from 31% to 54%, with a median survival time between 8.4 and 15.4 months and a 1-year survival rate between 30% and 59%. The GP schedule has acceptable toxicity. The GP combination can be considered standard therapy for non-small-cell lung cancer. Both 21- and 28-day schedules of GP were equally effective in non-small-cell lung cancer. It is necessary to study by randomized clinical trial, the sequence of cisplatin in GP schedule, the increase of radiosensitivity by gemcitabine, the combination of gemcitabine and other new chemotherapeutic agents.

Objective To develop and validate a qual it y of life(QOL) scale for the Chinese patients with uterocervical cancer (QOL-UC C). Methods Based on the internationally accepted de finition of QOL and the clinical characteristics of uterocervical cancer as wel l as the existing QOL scales, we designed the QOL-UCC. Then 113 patients were r ecruited and the reliability, validity and feasibility of the scale were tested. Results It was shown that the QOL-UCC had good reli ability, validity and feasibility. Conclusion QOL-U CC is a reliable and valid instrument for evaluation of the QOL in Chinese pa tients with uterocervical cancer.

Background and purpose:Hypoxic cells in tumors are of chemoresistant and anti-apoptoic.Our study was focused on the effects of hypoxia on expression of P-glycoprotein(P-gp) and cell apoptosis in human lung adenocarcinoma A549 cell line(wtP53),in order to explore the probable mechanism of hypoxia in apoptic tumor cells.Methods:A549 cell line(human lung adenocarcinoma) was cultured under hypoxic atmosphere(2% O_2) for 24 h,the expression of hypoxia inducible factor-1?、P-gp and P53 protein was detected by immunohistochemistry,after cells under hypoxic atmosphere were exposed to Adriamycin for 24 h,the cell survival rate was detected by MTT,and apoptosis of by flow cytometer.Results:① The expression of hypoxia inducible factor-1?、P-gp and P53 protein in hypoxic cells were higher than the expression in anoxia ,and correlation between the expression of HIF-1? and P-gp or P53 protein was observed(P

Objective To investigate the effect of histone deacetylases-1(HDAC-1)siRNA on the HDAC-1 protein expression,cell growth and apoptosis in HeLa cells.Methods The HDAC-1 protein was knocked down by HDAC-1 siRNA.HDAC-1 protein was detected by Western blot.The cell growth inhibition was assesses by MTT and clone forming assay.Apoptosis was measured by flow cytometry.Results Forty-eight h after transfection of HDAC-1 siRNA,HDAC-1 protein expression in HeLa cells was down-regulated obviously.The down-regulation of HDAC-1 significantly reduced the colony formation rate,inhibited cell proliferation and induced apoptosis in HeLa cells.Conclusion HDAC-1 siRNA may play an anti-proliferation role in HeLa cells by inducing apoptosis.

The expression of CD44v6 in 105 cases of advanced cervical cancer was detected by using im munohistoehemical method and the relation between adhesion molecule CD44v6 and prognosis in advanced cervical cancer was investigated. The experimental results showed that the lymphocytic infiltration and peritumor reaction of fibrin in the stroma were milder in the patients with CD44v6 positive than those with CD44v6 negative. There were significant reductions in overall and free relapse survival in the patients with CD44v6 positive as compared with those with CD44v6 negative. It was suggested that the expression of CD44v6 might serve as a new indicator for predicting the prognosis of advanced cervical cancer. Tumor cells might interfere with the recognition of the peritumor lymphocyte and reduce the peritumor reaction of fibrin in the stroma through the expression of CD44v6.

Objective To determine the reliability, validity and feasibility of using International Classifica-tion of Functioning, Disability and Health (ICF) core set for breast cancer in Chinese patients. Methods Quality of life (QOL) of 110 patients with breast cancer were investigated using ICF breast cancer core set and SF-36 scale. Statistical analysis tested their reliability, validity and feasibility. Results It was shown that the ICF core set for breast cancer had good reliability, validity and feasibility. Conclusions The ICF core set for breast cancer is a re-liable and valid instrument for Chinese breast cancer patients.

Objective To investigate the quality of life (QOL) of cervical cancer patients and its influential factors as well as to explore the method for improving the QOL in this population of patients. MethodsA QOL questionnaire for uterus cervical cancer (QOL-UCC) was designed and used in a survey of 81 UCC patients before they got any treatment and 124 UCC patients after they were treated for a period of time. SPSS 9.0 software was used to analyze data. ResultsThe patients got poor score with psychological status items(2.11? 0.34 ) before treatment, while those who had been treated for a period of time got poor score with social characteristics items(2.01? 0.33 ), especially with the work(1.87)and sexuality (1.00) items. Economic status of the patients, unsuccessful control of progress of the disease and recurrence were the main influential factors of the QOL in the UCC patients. ConclusionPsychosocial factors play very important roles in QOL of the UCC patients. As a result, psychosocial interventions should be stressed in the rehabilitation of this population of patients.

Objective To investigate the effects of ulinastatin on plasma ?-glucuronidase ?-GCD) activity, granulocyte elastase (Gel) and fibronectin (Fn) concentrations and the dose-effect relationships of ulinastatin with them during cardiopulmonary bypass (CPB) .Methods Thirty ASA Ⅱ - Ⅲ patients (19 male, 11 female) aged 3-29 yr, weighing 12-61 kg undergoing elective open heart surgery with CPB were randomly divided into two groups : ulinastatin group (U n - 15) and control group (C n = 12) . In group U patients received ulinastatin 200 000 U in 20 ml of normal saline (NS) infused iv over 10 min after induction of anesthesia and before CPB. An additional 200 000 U of ulinastatin was given iv if CPB lasted for more than 4 h. In control group NS was given iv instead of ulinastatin. Blood samples were taken before anesthesia and after CPB for determination of plasma ?-GCD activity and Gel and Fn levels. The differences in ?-GCD, Gel and Fn between baseline and post-CPB values were calculated (△?-GCD, △Gel, △Fn). In group C the correlation between △?-GCD and AGel;△?-GCD and △Fn; △Gel and △Fn and in group U the correlation of the dose of ulinastatin (U?kg-1 body weight) with △?-GCD /△Gel /△Fn were studied.Results (1) There was no significant difference in plasma ?-GCD activity, Gel and Fn levels between the two groups before anesthesia ( P

Objective To evaluate the effect of stereotactic radiotherapy combined with conventional radiotherapy for non-small cell lung cancer(NSCLC)patients with positive stump at the resected bronchial margin. Methods From June 1996 to November 2000, 41 NSCLC patients in whom microscopic residual disease found pathologically at the resected bronchial margin were treated by: conventional radiotherapy followed by stereotactic radiotherapy ( RT+SRT group 18 patients), while the other 23 patients received routine radiotherapy alone (RT group). Results The 1-,2-and 3-year local disease-free rates were better in RT+ SRT group (92.3%,~83.1% and 83.1%) than those in RT group (80.2%,60.2% and 39.5% ). However, no significant difference was found in the complication rate or survival rate between the two groups. Conclusions Stereotactic radiotherapy is effective as a boost irradiation to patients with non-small cell lung cancer with microscopic residual disease at the resected bronchial margin by improving the local control.

Objective To compare the influence of hypothyroid function in neonatal with hyperbilirubinemia by two kinds blue-light treatment way.Methods 36 cases were divided into the Bilibed infant blue light the bed treatment in 18 cases(group A) and the traditional warm box with the both blue light treatment in 18 cases(group B).The influence of hypothyroid function in neonatal with hyperbilirubinemia were observed after treatment and before treatment.Results The contents of bilirubin in two group after treatment were obviously lower than that before treatment(P0.05).Conclusion The therapeutic of Bilibed method can get the better therapeutic effect than the traditional warm box with the both blue light treatment for the neonatal with hyperbilirubinemia.The change of thyroid function was no influence.