1.Construction of human bone morphogenetic protein 2 and histidine eukaryotic expression plasmid and synthesis of chitosan/pIRES2-EGFP-hBMP2-His nanoparticles.
Xiaoyu YANG ; Shiyi LI ; Di ZHANG ; Ying WU ; Tao YANG ; Changhong LIU
West China Journal of Stomatology 2014;32(5):493-497
OBJECTIVETo clone and construct a eukaryotic expression vector of human bone morphogenetic protein (BMP) 2 and histidine in vitro and synthesize chitosan (CS)/pIRES2-EGFP-hBMP2-His nanoparticles.
METHODSpMD18T-hBMP2-His was digested by EcoR I and BamH I to obtain the hBMP2-His gene, which was inserted into pIRES2-EGFP to form pIRES2-EGFP-hBMP2-His. Afterward, CS, which exhibited five different molecular weights and deacetylation degrees, was complexed with pIRES2-EGFP-hBMP2-His to form CS/pIRES2-EGFP-hBMP2-His nanoparticles; in this procedure, a desolvent method was used at different N/P ratios (amino in CS to phospho in plasmid DNA). The gene-encapsulating ability of CS was evaluated by agarose gel electrophoresis and fluorescence spectrophotometry; size, distribution, and potential were analyzed using a ZetaPALS analyzer. The shape of the nanoparticles was observed under an atomic force microscope.
RESULTS1) pIRES2-EGFP-hBMP2-His was constructed after the cloned hBMP2-His gene was confirmed by sequencing. 2) CS/pIRES2-EGFP-hBMP2-His nanoparticles were synthesized and pIRES2-EGFP-hBMP2-His was packaged by CS. 3) CS/pIRES2-EGFP-hBMP2-His nanoparticles were globular with an average size of 111.7 nm to 3,214.2 nm and an average zeta-potential of 4.93 mV to 16.79 mV.
CONCLUSIONCS/pIRES2-EGFP-hBMP2-His nanospheres are successfully synthesized.
Bone Morphogenetic Protein 2 ; Chitosan ; Genetic Vectors ; Green Fluorescent Proteins ; Histidine ; Humans ; Nanoparticles ; Plasmids
2.The Timing of Return to Sports after Anterior Cruciate Ligament Reconstruction in Elite Athletes: A Study Based on Isokinetic Knee Muscle Strength and Multiple Hop Tests
Tao YANG ; Jingbin ZHOU ; Tianwu CHEN ; Shiyi CHEN ; Guoping LI
Chinese Journal of Sports Medicine 2018;37(3):185-191
Objective To compare the knee function recovery at different times of returning to sport after anterior cruciate ligament reconstruction(ACLR) among elite athletes using knee isokinetic muscle strength test and various hop test.Methods Forty-one elite athletes(14 males,27 females,mean age 22.6 ± 4.1 years) undergoing ACLR between January 2013 and September 2014 were chosen from the database of the National Institute of Sports Medicine and Shanghai Huashan Hospital.Rehabilitation was performed using the same protocol by professional physiotherapists and trainers,who recorded the time of returning to sport of each athlete.One week prior to the scheduled return,bilateral knee isokinetic muscle strength test(test value:peak torque;angle velocity:60°/s,180°/s;motion:flexion,extension) and four hop tests(single hop for distance,side-to-side hop,up-down hop and 8 hop) were applied with the limb symmetry index(LSI) calculated.The athletes were then grouped by their returntime referring to surgery into the premature group(6~8 months),timely return group(9~12 months) and delayed group(over 12 months).The tests results were recorded and compared among the three groups.Results Fourteen athletes were selected into the premature group,with 19 in the timely group and 8 in the delayed group.The average LSI of 60°/s flexion peak torque of the premature group (87.4% ± 7.5%) was significantly lower than the timely group(95.8% ± 6.6%) and the delayed group(96.0% ± 2.4%) (P<0.01).Significant differences were observed between the premature group and delayed group regarding the 60° extension peak torque(85.8% ± 9.4% and 94.8% ± 4.8%,P<0.05),180°/s flexion peak torque(90.7% ± 8.7% and 101.4% ± 6.8%,P<0.05),and 180°/s extension peak torque (90.6% ± 5.2% and 97.8% ± 5.6%,P<0.05).The average LSL of the premature group at single hop for distance,side-to-side hop and up-down hop(93.A% ± 8.5%,84.7% ± 7.3% and 112.5% ± 5.7%) was significantly lower than that of the timely group(95.7% ± 6.0%,104.2% ± 4.3% and 105.3% ± 7.9%) and the delayed group regarding(98.1% ± 1.9%,104.7% ± 4.0% and 106.3% ± 7.4%) (P<0.01 for all).The relative peak torque of 60°/s extension of the premature group(2.48 ± 0.58 Nm/kg) was significantly lower than the delayed group(3.21 ± 0.51 Nm/kg) (P<0.01).Conclusions For elite athletes,returning to sport within 9 months after ACLR results in insufficient restoration of the knee function.Delayed return to sport doesn't improve the outcomes of hop tests,but can enhance the maximum extension torque peak,which needs further study.
3.Research Progress of Biodegradable Vascular Stent.
Yangyang WANG ; Hongmei WU ; Wenqiang ZHEN ; Tao GONG ; Duan LI ; Yao CAI ; Shiyi FAN
Chinese Journal of Medical Instrumentation 2021;45(4):410-415
Biodegradable vascular stents have better biocompatibility than drug-eluting stents. The blood vessels are rebuilt and degraded after normal physiological functions are restored. Due to it will not stay in the body for a long time and the patients don't need taking anti-rejection drugs all the time, it becomes the focus of attention in the treatment of coronary heart disease. This article introduced the development history of biodegradable stents and reviewed the research status of several different materials of vascular stents (animals or humans)
Absorbable Implants
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Animals
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Drug-Eluting Stents
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Humans
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Stents
4.Clinical expert consensus on platelet-rich plasma treatment for lateral epicondylitis (2022 version)
Jian LI ; Guoqing CUI ; Chengqi HE ; Shiyi CHEN ; Boxu CHEN ; Hong CHEN ; Xuesong DAI ; Hongchen HE ; Hui KANG ; Tieshan LI ; Guoping LI ; Jiuzhou LU ; Chao MA ; Xin TANG ; Jun TAO ; Hong WANG ; Ming XIANG ; Dan XING ; Yiquan XIONG ; Qingyun XUE ; Rui YANG ; Tin YUAN ; Qiang ZHANG ; Jingbin ZHOU ; Weihong ZHU ; Yan XIONG ; Yan LIU
Chinese Journal of Trauma 2022;38(8):673-680
Lateral epicondylitis is a common clinical disease with characteristics of lateral elbow pain, insidious onset and easy recurrence, which can cause forearm pain and decreased wrist strength, seriously affecting patients′ daily life and work. Although there are various treatment methods for lateral epicondylitis with different effects, standard treatments are still lacking nowadays. Platelet-rich plasma (PRP) has good effects on bone and tendon repair, and is now widely used in the treatment of lateral epicondylitis. However, there is a lack of a unified understanding of the technology and specifications of PRP in the treatment of lateral epicondylitis. Therefore, the Sports Medicine Branch of the Chinese Medical Association and Physical Medicine and Rehabilitation Branch of the Chinese Medical Association organized experts in the fields of sports medicine and rehabilitation medicine in China to formulate the "clinical expert consensus on platelet-rich plasma treatment for lateral epicondylitis (2022 version)", and proposed suggestions based on evidence-based medicine mainly from the concept, epidemiology and pathophysiology of lateral epicondylitis, symptoms, signs and imaging manifestations of lateral epicondylitis, PRP concept and application component requirements, quality control of PRP preparation technology, indications and contraindications of PRP in the treatment of lateral epicondylitis, PRP injection in the treatment of lateral epicondylitis, application of PRP in the operation of lateral epicondylitis, related problems after PRP treatment of lateral epicondylitis, evaluation of the results after PRP treatment of lateral epicondylitis, and health and economic evaluation of PRP treatment of lateral epicondylitis, so as to provide guidance for clinical diagnosis and treatment.
5.Establishment and Validation of Clinical Prediction Model for 1-year MACEs Risk After PCI in CHD Patients with Blood Stasis Syndrome
Shiyi TAO ; Lintong YU ; Deshuang YANG ; Gaoyu ZHANG ; Lanxin ZHANG ; Zihan WANG ; Jiarong FAN ; Li HUANG ; Mingjing SHAO
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(20):69-80
ObjectiveTo establish and validate a clinical prediction model for 1-year major adverse cardiovascular events(MACEs)risk after percutaneous coronary intervention (PCI) in coronary heart disease (CHD) patients with blood stasis syndrome. MethodThe consecutive CHD patients diagnosed with blood stasis syndrome in the Department of Integrative Cardiology at China-Japan Friendship Hospital from September 1, 2019 to March 31, 2021 were selected for a retrospective study, and basic clinical features and relevant indicators were collected. Eligible patients were classified into a derivation set and a validation set at a ratio of 7∶3, and each set was further divided into a MACEs group and a non-MACEs group. The factors affecting the outcomes were screened out by least absolute shrinkage and selection operator (Lasso) and used to establish a logistic regression model and identify independent prediction variables. The goodness-of-fit of the model was evaluated by the Hosmer-Lemeshow test, and the area under curve (AUC) of the receiver operating characteristic (ROC) curve, calibration curve, decision curve analysis (DCA), and clinical impact curve (CIC) were employed to evaluate the discrimination, calibration, and clinical impact of the model. ResultA total of 731 consecutive patients were assessed and 404 eligible patients were enrolled, including 283 patients in the derivation set and 121 patients in the validation set. Lasso identified ten variables influencing outcomes, which included age, sex, fasting plasma glucose (FPG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), homocysteine (Hcy), brachial-ankle pulse wave velocity (baPWV), flow-mediated dilatation (FMD), left ventricular ejection fraction (LVEF), and Gensini score. The multivariate Logistic regression preliminarily identified age, FPG, TG, Hcy, LDL-C, LVEF, and Gensini score as the independent variables that influenced the outcomes. Of these variables, male, high FMD and high LVEF were protective factors, and the rest were risk factors. The prediction model for 1-year MACEs risk after PCI in CHD patients with blood stasis syndrome showed χ2=12.371 (P=0.14) in Hosmer-Lemeshow test and the AUC of 0.90. With the threshold probability > 10%, the model showed better prediction performance for 1-year MACEs risk after PCI in CHD patients with blood stasis syndrome than for that in all the patients. With the threshold probability > 60%, the estimated value was much closer to the real number of patients. ConclusionThe established clinical prediction model facilitates the early prediction of 1-year MACEs risk after PCI in CHD patients with blood stasis syndrome, which can provide ideas for the precise treatment of CHD patients after PCI and has guiding significance for improving the prognosis of the patients. Meanwhile, multi-center studies with larger sample sizes are expected to further validate, improve, and update the model.