53 male Wistar rats were divided into three groups, i.e. experimental ulcer group, saline control group and normal control group. The antral tissues were prepared for immunohistochemical staining at 4th, 10th, 14th, 21st and 28th days after operation. Sternberger's PAP method was used to demonstrate the gastrin cells (G cells) and somatostatin cells (D cells)in the antral mucosa in order to observe their changes during experimental gastric ulcer. The morphological relationships of G cells and D cells were examined by simultaneous double immunostaining method.The results indicated that the G cells count as well as D cells count in the antral mucosa was increased (P

Objective:To explore the resistance of common clinical isolates to chlorhexidine gluconate (CHG) and the clinical characteristics of patients with the infections.Methods:A total of 1 000 isolates from the First Affiliated Hospital of Wenzhou Medical University in 2018 (from January to May) were collected, which included 200 strains each of Escherichia coli ( E. coli), Acinetobacter baumanii ( A. baumanii), Pseudomonas aeruginosa ( P. aeruginosa), Staphylococcus aureus ( S. aureus), and Enterococcus spp.. Minimum inhibitory concentration (MIC) of CHG against 1 000 isolates were determined by the agar dilution method. The correlation between the resistance of isolates and clinical characteristics of infected patients was analyzed. Chi-square test or Fisher exact probability test were used for statistical analysis. Results:A total of 57 CHG resistant strains were detected in 1 000 clinical isolates. These CHG-resistant strains were mainly isolated from sputum and intensive care unit ward, accounting for 49.1%(28/57)and 38.6%(22/57), respectively. The resistance rates of P. aeruginosa, A. baumanii, Enterococcus spp., S. aureus, and E. coli to CHG were 16.0%(32/200), 7.0%(14/200), 3.0%(6/200), 1.5%(3/200) and 1.0%(2/200), respectively. The CHG-resistant rates of P. aeruginosa to ceftazidime, ciprofloxacin, levofloxacin and gentamicin were 53.1%(17/32), 78.1%(25/32), 65.6%(21/32) and 50.0%(16/32), respectively, which were all higher than those of CHG-sensitive P. aeruginosa (25.0%(8/32), 25.0%(8/32), 21.9%(7/32) and 15.6%(5/32), respectively), with statistical significance ( χ2=5.317, 18.080, 12.444 and 8.576, respectively, all P<0.05). The hospital mortality was 22.8%(13/57) in patients infected with CHG-resistant bacteria, which was higher than that in patients infected with CHG-sensitive bacteria ((7.0%(4/57); Fisher exact probability test, P=0.018)). CHG-resistant group had a higher history of CHG exposure and antimicrobial treatment (61.4%(35/57) and 70.2%(40/57), respectively), which were both higher than those with CHG-susceptible isolates (17.5%(10/57) and 47.4%(27/57), respectively), the differences were both statistically significant ( χ2=22.947 and 6.118, respectively, both P<0.05). In addition, the multi-drug resistance rate of CHG-resistant strains was 54.4%(31/57), which was higher than that of CHG-susceptible strains (35.1%(20/57)), the difference was statistically significant ( χ2=4.293, P=0.039). Conclusions:CHG resistant strains have higher antimicrobial resistance. Hospital mortality in patients infected with CHG-resistant bacteria is higher than patients infected with CHG-sensitive bacteria. The important risk factors are CHG exposure and antimicrobial therapy.

Clinically,arteriovenous malformations (AVM) is a common intracranial vascular disease.Traditional treatments for cerebral AVM include microsurgical resection,endovascular embolization and radiotherapy.However,there are some unusual AVM lesions that are difficult to be cured by traditional methods.Multiple case reports that have been published recently indicate that embolization therapy via transvenous approach is very effective for these unusual AVM lesions,especially for small hemorrhagic AVMs.These lesions often have single vein drainage and are located at deep cerebral function area.with their blood supply being from fine arteries.This paper aims to review the existing literature and to make a summary about the indications,method of operation,risks and prevention,etc.of embolization therapy via transvenous approach for cerebral AVM.

PURPOSE: We evaluated the usefulness of intracranial stent implantation for treating patients with atherosclerotic stenosis and with recurrent, ischemic, neurological symptoms despite having undergone medical therapy. MATERIALS AND METHODS: Between March 2004 and April 2010, we attempted intracranial, stent-assisted angioplasty in 77 patients with 85 lesions (anterior circulation 73 cases, posterior circulation 12 cases) and who had ischemic neurological symptoms with more than 50% major cerebral artery stenosis. We analyzed the results regarding the technical success rate, complication rate, and restenosis rate during the mean 29.4 month follow-up period. RESULTS: Intracranial stent implantation was successfully performed in 74 cases (87.1%). In nine cases among the 11, failed cases, stent implantation failure was due to the tortuosity of the target vessel. One patient experienced middle cerebral artery rupture during the procedure, and we embolized the vessel using a microcoil. Five patients developed cerebral infarction in three weeks after the procedure, three of whom improved using conservative management, although the other, two patients expired. The mean number of residual stenoses decreased from 72.3% to 14.7%. Three patients demonstrated significant in-stent restenosis, i.e. more than 50%, during the follow-up period. CONCLUSION: As stent-assisted angioplasty in intracranial, atherosclerotic stenosis is effective and relatively safe, it can be considered as an alternative treatment for patients with recurrent, ischemic, neurologic symptoms despite having undergone medical therapy.
Angioplasty ; Cerebral Arteries ; Cerebral Infarction ; Constriction, Pathologic ; Follow-Up Studies ; Glycosaminoglycans ; Humans ; Intracranial Arteriosclerosis ; Middle Cerebral Artery ; Neurologic Manifestations ; Rupture ; Stents

The 2022 Fudan university doctorate academic public health forum hosted by the graduate school of Fudan university， the graduate work department of the party committee of Fudan university， and the school of public health of Fudan university was successfully held on December 10， 2022 in the school of public health of Fudan university. In the early stage， a total of 53 manuscripts from nationwide universities and research institutes were received， and 10 were selected as excellent papers to participate in the forum report. More than 100 teachers and students attended the forum. Focusing on the theme of " Adhering to the original ambition of scientific research to serve the country and practicing the mission of public health youth"， the major contents of the forums included excellent paper reports in the morning and round-table discussion in the afternoon. Experts and students conducted discussions and communications on prevention， control and management of public health emergencies. It is of great benefit for students to uphold the original intention，practice the mission and further improve public health research.

Animals ; Dogs ; Drug Resistance, Viral ; drug effects ; Female ; Humans ; Influenza A Virus, H7N9 Subtype ; Madin Darby Canine Kidney Cells ; Male ; Orthomyxoviridae Infections ; drug therapy ; Ribavirin ; pharmacology