1.Effects of glial cell derived neurotrophic factor on colon glial cells in rats with slow transit constipation
Ning JIANG ; Fang YAO ; Shiyi WANG ; Yihong FAN ; Bing LYU
Chinese Journal of Digestion 2016;36(6):403-406
Objective To investigate the effects of exogenous glial cell derived neurotrophic factor (GDNF)on colon glial cells in slow transit constipation (STC ) rats,and to explore the optimal concentration of GDNF in order to provide evidence for intestinal neurotrophic therapy in the treatment of STC.Methods A total of 132 SD rats were divided into STC group and control group,66 rats in each group.STC rats were established by feeding with rhubarb.Six rats were randomly selected from either groups to verify whether STC model was successfully established.And the left 120 rats of two groups were randomly divided into six subgroups:STC group one to group six and control group one to group six,ten rats in each group,which were untreated,injected through tail vein with saline,and 0.001 ,0.010, 0.050,0.100 μg/L GDNF 2 mL respectively for one week.The expression of Sox-8 at protein level of either group were detected by Western blotting.Independent sample t test was performed for statistical analysis.Results After treated with 0.001 μg/L GDNF (STC group three),there was no significant
difference in expression level of Sox-8 between STC group three and STC group one (13.38 ±0.70 vs 13.39±0.45 ,t = 0.042,P = 0.969 ).After treated with 0.010 μg/L GDNF (STC group four),the difference in expression level of Sox-8 between STC group four and STC group three was significant (21 .11 ±2.56 vs 13.38±0.70,t=5 .040,P <0.01).After treated with 0.050 μg/mL GDNF (STC group five),the expression level of Sox-8 was higher than that in STC group four (31.86±1.57 vs 21.11±2.56,t=-6.198,P <0.01 ).The Sox-8 expression of untreated,saline treated,0.001 and 0.050 μg/L GDNF treated STC rats (STC group one,two,three and five)were lower than those of the corresponding control groups (t= 3.394,12.103,10.302,- 6.120,all P < 0.05 ).Conclusion Exogenous GDNF could increase Sox-8 expression in colon tissue of STC rats,an increase in the number of colon glial cells could repair enteric nervous system,and 0.050 μg/L was the optimal concentration.
2.Therapeutic Efficacy of Shengxuening Tablets in the Treatment of Iron-deficiency Anemia during Pregnancy:A Meta-analysis
Fan CHEN ; Weiwei LIU ; Qiaoyan LIU ; Shiyi CAO
China Pharmacy 2018;29(12):1707-1710
OBJECTIVE:To evaluate therapeutic efficacy of Shengxuening tablets in the treatment of iron-deficiency anemia during pregnancy,so as to provide evidence for clinical decisions. METHODS:Retrieved from CNKI,Wanfang,VIP and PubMed,clinical randomized controlled trials(RCTs)about Shengxuening tablets in the treatment of iron-deficiency anemia during pregnancy were collected during 2008-2018. After screening literatures and extracted information, the literature quality was evaluated by using improved Jadad scale. Meta-analyses was performed by using Stata 12.0 software. RESULTS:A total of 11 RCTs were included,inulving 1 617 patients. Total response rate of Shengxuening tablets was significantly higher than those of ferrous sulfate [OR=8.73,95%CI(2.964,25.69),P<0.05],ferrous succinate [OR=1.86,95%CI(1.04,3.33),P<0.05] and dietory conditioning [OR=3.43,95%CI(2.08,5.66),P<0.05],with statistical significance. CONCLUSIONS:Therapeutic efficacy of Shengxuening tablets is significantly better than that of routine iron supplements in the treatment of iron-deficiency anemia during pregnancy.
3.permanent crown-root caries in 35-44-year-old population in Guangdong Province (2015-2016)
LI Shiyi ; LI Jianbo ; FAN Weihua ; ZHAO Wanghong ; HUANG Yanmei ; HUANG Shaohong
Journal of Prevention and Treatment for Stomatological Diseases 2017;25(11):702-705
Objective:
To investigate the current status of caries on permanent teeth in adult population at the age of
35⁃44 years old in Guangdong Province, thus to provide scientific basis for the establishment of oral health care policies
in Guangdong.
Methods:
An equal⁃sized stratified multi⁃stage randomly sampling design was applied to obtain represen⁃
tative sample groups consisted of 288 Guangdong residents each, aged at 35⁃44 years old, with a gender ratio of half to
half. The caries on the crowns and roots of permanent teeth were assessed according to The Guideline for the 4th National
Oral Health Survey; thereafter the prevalence and mean DFT (decayed and filled tooth) of permanent teeth were calculat⁃
ed. The data obtained were analyzed using SAS9.2 package.
Results:
In 35⁃44 year ⁃old population, the prevalence of
crown caries was 71.18%, with a mean DFT of 2.76, and a filled rate of 36.78%; while the prevalence of root caries was
28.47%, with a mean DFT of 0.66, and a filled rate of 4.23%. The prevalence of caries of crown and root and mean DFT of
crown caries were higher in countryside when compared to the urban opponents. And female had higher prevalence and
mean DFT score in crown and root caries when compared to male. However, the mean DFT score of root caries in urban
was almost the same as that in countryside.
Conclusion
There was a high level of crown caries in Guangdong. Although
the prevalence of root caries is low, most of the involved roots was not filled.
4.Research Progress of Biodegradable Vascular Stent.
Yangyang WANG ; Hongmei WU ; Wenqiang ZHEN ; Tao GONG ; Duan LI ; Yao CAI ; Shiyi FAN
Chinese Journal of Medical Instrumentation 2021;45(4):410-415
Biodegradable vascular stents have better biocompatibility than drug-eluting stents. The blood vessels are rebuilt and degraded after normal physiological functions are restored. Due to it will not stay in the body for a long time and the patients don't need taking anti-rejection drugs all the time, it becomes the focus of attention in the treatment of coronary heart disease. This article introduced the development history of biodegradable stents and reviewed the research status of several different materials of vascular stents (animals or humans)
Absorbable Implants
;
Animals
;
Drug-Eluting Stents
;
Humans
;
Stents
5.Establishment and Validation of Clinical Prediction Model for 1-year MACEs Risk After PCI in CHD Patients with Blood Stasis Syndrome
Shiyi TAO ; Lintong YU ; Deshuang YANG ; Gaoyu ZHANG ; Lanxin ZHANG ; Zihan WANG ; Jiarong FAN ; Li HUANG ; Mingjing SHAO
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(20):69-80
ObjectiveTo establish and validate a clinical prediction model for 1-year major adverse cardiovascular events(MACEs)risk after percutaneous coronary intervention (PCI) in coronary heart disease (CHD) patients with blood stasis syndrome. MethodThe consecutive CHD patients diagnosed with blood stasis syndrome in the Department of Integrative Cardiology at China-Japan Friendship Hospital from September 1, 2019 to March 31, 2021 were selected for a retrospective study, and basic clinical features and relevant indicators were collected. Eligible patients were classified into a derivation set and a validation set at a ratio of 7∶3, and each set was further divided into a MACEs group and a non-MACEs group. The factors affecting the outcomes were screened out by least absolute shrinkage and selection operator (Lasso) and used to establish a logistic regression model and identify independent prediction variables. The goodness-of-fit of the model was evaluated by the Hosmer-Lemeshow test, and the area under curve (AUC) of the receiver operating characteristic (ROC) curve, calibration curve, decision curve analysis (DCA), and clinical impact curve (CIC) were employed to evaluate the discrimination, calibration, and clinical impact of the model. ResultA total of 731 consecutive patients were assessed and 404 eligible patients were enrolled, including 283 patients in the derivation set and 121 patients in the validation set. Lasso identified ten variables influencing outcomes, which included age, sex, fasting plasma glucose (FPG), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), homocysteine (Hcy), brachial-ankle pulse wave velocity (baPWV), flow-mediated dilatation (FMD), left ventricular ejection fraction (LVEF), and Gensini score. The multivariate Logistic regression preliminarily identified age, FPG, TG, Hcy, LDL-C, LVEF, and Gensini score as the independent variables that influenced the outcomes. Of these variables, male, high FMD and high LVEF were protective factors, and the rest were risk factors. The prediction model for 1-year MACEs risk after PCI in CHD patients with blood stasis syndrome showed χ2=12.371 (P=0.14) in Hosmer-Lemeshow test and the AUC of 0.90. With the threshold probability > 10%, the model showed better prediction performance for 1-year MACEs risk after PCI in CHD patients with blood stasis syndrome than for that in all the patients. With the threshold probability > 60%, the estimated value was much closer to the real number of patients. ConclusionThe established clinical prediction model facilitates the early prediction of 1-year MACEs risk after PCI in CHD patients with blood stasis syndrome, which can provide ideas for the precise treatment of CHD patients after PCI and has guiding significance for improving the prognosis of the patients. Meanwhile, multi-center studies with larger sample sizes are expected to further validate, improve, and update the model.