【Objective】 To conduct serological and molecular study of D^el type in RhD-negative donor population in Zhongshan area, so as to improve the diagnosis of D^el type. 【Methods】 A total of 102 initially RhD-negative samples, collected from December 2017 to February 2019, were classified by RHCE and PCR-SSP genotyping. And 95 cases of truly negative RhD were confirmed by IAT, 28 cases of D^el type were identified by absorption and elution test. The phenotype and genotyping characteristics of D^el type in Zhongshan area were summarized based on domestic data of relative literature. 【Results】 Among 102 initially RhD-negative samples by serological test, 95 were truely RhD-negative, 28 were DELRHD 1227A without any other D^el allele. Among them, RHCE antigen type were Ccee in 20(71.4%) cases, CCee in 8(28.6%), with no difference in comparison with other regions in China. The frequency of D^el in RhD-negative blood donors was 29.5% (28/95), with difference between Shanghai, Taiwan, and Fuzhou, but no difference between Nanchang, Zhejiang, and Wuhan. 【Conclusion】 The study showed that the D^el phenotype was closely related to Ce haplotype, and has no difference with other regions in China. The frequency of D^el type in RhD negative donors was 29.5%, with regional differences. RHD1227A was the main allele of D^el.