1.THE EFFECTS OF TESTOSTERONE ON MITOCHONDRIA Ca~(2+)-ATPase ACTIVITY IN NON-ISCHEMIC AREA FOLLOWING MYOCARDIAL INFARCTION IN RAT
Shiwen SONG ; Wannian ZHANG ; Zhu LIU
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
The effects of testosterone on the mitochondria Ca~(2+)—ATPase activity of uninfarcted ventricular septum following myocardial infarction (MI) was observed dynamically by using the experimental MI model in rat. The results showed that on l day after MI the mitochondria Ca~(2+)—ATPase activity of non—ischemic area was more higher than that of the normal control group (p
2.MRI Analysis of Morphological Structure in Protrusion of cervical Disc under Different Directions of Traction
Shiwen LIU ; Hongchen SONG ; Guoku ZHAO
Chinese Journal of Sports Medicine 2001;20(1):46-47,49
Objective The purpose of this study was to compare the functional and morphological changes in the cervical spine with protrusion of cervical disc (PCD) under different directions of traction. Methods MRI scanning was used to study the effect of different directions of traction on the movement of protrusive disc,compression index of spinal cord and changes of intervetebral space,as well as the cases with degeneration. Results Differences were found in the effect of different directions of traction. Most significant partial reduction of the protrusive disc was experienced in flexion traction. Both flexion and extension traction caused significant decrease in the index of compression of spinal cord. Extension traction caused marked narrowing of the posterior intervertebral space in the group with decreased disc size,while no difference between the groups with and without decreased disc size when given flexion traction. There was no difference in the anterior intervertebral space between the two directions of traction. No morphological change was observed in cases with degeneration using either direction of traction. Conclusion PCD could caused the change in the fine morphology of normal vertebral joints. The traction direction should be chosen according to the condition of the individual case. The compression index could better reflect the nature of spinal cord injury than the size of the disc protrusion. Traction would not give much benefit in cases with degeneration.
3.Effect of Early Reinforced Control Ability Training of the Trunk and Pelvis on the Motor Function of Patients with Stroke
Shuchang ZHENG ; Shiwen ZHU ; Chengzhong SONG ; Ping MA ; Ruiwei JIA
Chinese Journal of Rehabilitation Theory and Practice 2007;13(8):716-717
Objective To observe the effect of the early reinforced control ability training of the trunk and pelvis on the motor function of patients with stroke.MethodsFifty-six patients with stroke were randomly divided into the therapy group and control group with 28 cases in each group. The patients of two groups were treated with regular rehabilitation training, forty-five minutes at a time, twice one day; at the same time the patients of the therapy group were administrated with the control ability training in the trunk and pelvis, twenty minutes at a time, twice one day. The Fugl-Meyer Assessment scale (FMA), the Barthel Index (BI) and functionality ambulation category (FAC) were used to assess the motor function of the patients, before and after the treatment.ResultsTwo months after treatment, there was a significant increasing in the FMA, BI, FAC and hemiplegia gait analysis assessment in the both groups ( P<0.05~0.01), but the walking ability of the therapy group was significantly better than that of the control group ( P<0.05).ConclusionCombined the early reinforced control ability training of the trunk and pelvis with routine rehabilitation training is obvious effect on the motor function of the patients with stroke.
4.Observation of effects of the brain-located mild hypothermia therapy on acute progressive cerebral infarction
Shiwen ZHU ; Qinghua REN ; Yizhao LI ; Chengzhong SONG ; Yuanbiao SUN
Chinese Journal of Rehabilitation Theory and Practice 2003;9(6):326-327
ObjectiveTo study effects of treating acute progressive cerebral infarction by the brain-located mild hypothermia therapy.Methods60 patients were randomly divided into the treatment group (30 cases) and the control group (30 cases). Drugs were used in both groups, and the located mild hypothermia therapy was only applied in the treatment group. After 72 hours and 7 days, the volume of cerebral infarction and the nerve function deficiency (NFD) scores of hemiplegic limbs were compared.ResultsThe volume of cerebral infarction of the treatment group was significantly smaller than that of the control group (P<0.01).There were significant differences on the neurological impairment scores between two groups (P<0.01).Conclusions The brain-located mild hypothermia therapy can reduce hydrocephalus of patients with acute progressive cerebral infarction, and have brain protection. These results provide an important theoretical foundation for the treatment of cerebral infraction with low-temperature therapy.
5.Experimental and clinical study on diagnosis of acute intestinal ischemia in early stage
Shiwen WANG ; Jinchun HE ; Ailin SONG ; Linyan WANG
Chinese Journal of General Surgery 2001;0(08):-
ObjectiveTo study the diagnostic value of alteration of serum enzyme in acute intestinal ischemia(AII). MethodsChanges of serum levels of CPK, CKMB, LDH, LA, CRP and CO2CP of venous blood of 40 rabbits and 53 patients in different ischemia conditions at different time before and after operation were measured to determine the relationship between the changes and ischemia degree of intestine. ResultsThe serum levels of CPK, CKMB, LDH, LA and CRP increased gradually with the severity of AII and decreased with the improvement of AII, but CO2CP was the reverse.The 6 assay values mentioned above in animals of AII compared with the control group, and in patients in pretreatment compared with the control group, and in reversible intestinal necrosis compared with irreversible intestinal necrosis, and in nonoperation group 1 h after hospitalization compared with reversible intestinal necrosis group 2 h before operation(except CO2CP)all had remarkable difference(P
6.Effect of Constraint-induced Movement Therapy Combined with Motor Imagery Therapy on the Upper Extremity Function of Hemiplegic Patients
Shiwen ZHU ; Yizhao LI ; Chengzhong SONG ; Zunwei XIE ; Rongyan CHEN ; Fei XUAN ; Jinli ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2007;13(2):131-132
ObjectiveTo observe the effect of constraint-induced movement therapy (CIMT) combined with motor imagery therapy (MIT) on the upper extremity function of stroke patients with hemiplegia.Methods120 hemiplegic patients caused by brain injury were randomly divided into the control group, CIMT group, MIT group and combined therapy group with 30 cases in each group. The every group was treated with commensurate rehabilitation management respectively, 4 weeks as one course of treatment. All patients were assessed with the Simple Test for Evaluating Hand Function (STEF), the upper limb movement Fugl-Meyer Assessment (FMA), and Modified Barthel Index (MBI) before and after the treatment.ResultsBefore the treatment, there was no significant difference in STEF, scores of upper limb movement FMA and MBI among the four groups ( P>0.05). After the treatment, the STEF, scores of upper limb movement FMA and MBI of the CIMT group and MIT group improved ( P<0.05), that of the combined therapy group were superior to any other group ( P<0.01).ConclusionCIMT and MIT can promote the recovery of upper extremity function of hemiplegic patient, but the better therapeutic effect will be obtained when these two therapies combined.
7.Effect of ivaradine on hyperpolarization activated cation current in canine pulmonary vein sleeve cardiomyocytes with atrial fibrillation
Yang LI ; Yan HUANG ; Zongbin LI ; Hao WANG ; Jianguo SONG ; Yuqi LIU ; Lei GAO ; Shiwen WANG
Journal of Geriatric Cardiology 2008;5(1):39-42
Objective To study the effect of ivabradine on hyperpolarization activated cation current in canine pulmonary vein(PY) sleeve cardiomyocytes with atrial fibrillation.Methods Dissociation of PVs yielded single cardiomyocytes from a Landengorff column without or with pacemaker activity from long-term rapidly atrial pacing (RAP) canines.If current was measured with the whole-cell patch-clamp technique.Results Compared with the control group,the rapidly atrial pacing canine PV cardiomyocytes had spontaneous diastolic depolarization and had larger If densities.Ivabradine (Iva,1 μM),a selective inhibitor of the If current,markedly reduced If currents in the RAP from -2.66±0.4 pA/pF to -1.58±0.1 pA/pF at the test potential of-120 mV (P<0.01,n=12).Inhibition effect of Iva of If current showed concentration-dependent range from 0.1 to 10.0μM,with IC50 of 2.2 μ M ( 1.8-2.9 μM,95% CL).Furthermore,V1/ of steady-state activated curve was shifted from -84.3±4.9 mV to -106.9±3.4 mV and k value of steady-state activated curve was changed from 12.1+2.6 mV to 9.9±3.4 mV by the application of.1.0 μM Iva ( P<0.01,n=12).Conclusions Our study revealed that Ivarbadine may significantly decrease If of rapidly atrial pacing pulmonary vein sleeve ceUs with atrial fibdllation.(J Geriatr Cardiol 2008;5:39-42)
8.Autologous Mesenchymal Stem Cell Transplantation in Patients with Diseases of Nervous System
Gui-xiang CUI ; Yi-zhao LI ; Hua Hua ; Shiwen ZHU ; Chengzhong SONG ; Zunwei XIE ; Xiaoxia ZHANG ; Qing SHI ; Huaishui HOU ; Bojun SHEN
Chinese Journal of Rehabilitation Theory and Practice 2006;12(3):242-245
ObjectiveTo investigate the clinical effects and side-effect of mesenchymal stem cell(MSCs) transplantation on spinal cord injury(SCI),traumatic brain injury(TBI),multiple sclerosis(MS) or Parkinson's disease(PD).MethodsThe bone marrow(222~350 ml) of 11 patients with SCI(n=6),TBI(n=3),MS(n=1) or PD(n=1) were harvested from the patients' ilia and then MNCs were isolated.The MNCs were injected intravenously or into subarachnoid space by lumbar puncture.The neural function and side-effect were observed before and after MSCs transplantation and the patients were followed up.ResultsThe data demonstrated the improvement of sense and motor function in 5 patients with SCI,one had no improvement by 2 months following-up.These patients' sense and motor levels improved obviously.Their muscle strength of lower extremity increased,the muscular tone decreased and urinary bladder function improved.Changes in neurological deficits and improvements in function may appear within 2 days after transplantation,most of them within 2 weeks.There were significantly amelioration in 3 patients with TBI treated with MSCs transplantation,one of them could walk with cane independently after 3 months.One's PVS score elevated from 5 to 8 scales after transplantation.The tremor was alleviated after 1 week,and the muscular tone decreased,which lead to reduce the dose of Madopar after 3 months,in patient with PD.The patient with MS showed no improvement in short time.The side-effect included fever(7/11),headache(2/11) and abdominal dissension(1/11).1 patient feel numb in his legs while injection into subarachnoid,and appeared meningeal stimulation after injection.ConclusionThere were significantly clinical effects in treatment of SCI,TBI,MS,and PD with MSCs transplantation in short time,and with few side-effect. The long-term clinical effects need more observation with larger samples.
9.Surgical freedom of odontoidectomy via endoscopic endonasal approach
Ruichun LI ; Chen LIANG ; Shiwen GUO ; Cheng YANG ; Minxue LIAN ; Qian SONG ; Baixiang HE ; Xu YANG ; Gang BAO
Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(3):345-348
【Objective】 To quantitatively analyze the surgical freedom of odontioectomy via endoscopic endonasal approach. 【Methods】 Seven adult head specimens were dissected by the endoscopic transnasal approach to the sellar region and craniocervical junction. The center of sellar floor (CenSF), opticocarotid recess (LOCR), foramen magnum, atlas, atlas-occipital joint and tip of odontoid process (TOP) were exposed. The surgical freedom of TOP was calculated by using the spatial coordinate positioning system of neuronavigation, and compared with that of LOCR and CenSF. 【Results】 CenSF and LOCR were common landmarks in the endonasal endoscopic approach. When the surgical freedom between TOP and CenSF and LOCR was compared, it indicated that ① The angle of attack on axial plane (AAAP):There was a significant difference among TOP, LOCR and CenSF (5.7 ° vs. 6.9 ° vs. 8.5 °, P=0.004). The comparison between the two groups showed that TOP was less than CenSF (P=0.003). ② The angle of attack on sagittal plane (AASP): There was a significant difference among TOP, LOCR and CenSF (6.3° vs. 7.0° vs. 9.5°, P=0.009). The TOP was less than CenSF (P=0.008). ③ There was no statistical significance between TOP and LOCR in surgical freedom (P=0.604, P=0.688). 【Conclusion】 Endoscopic transnasal approach can provide sufficient surgical freedom for odontoidectomy.
10.Full-profile pharmacokinetics, anticancer activity and toxicity of an extended release trivalent PEGylated irinotecan prodrug.
Shiwen SONG ; Dong SUN ; Hong WANG ; Jinliang WANG ; Huijing YAN ; Xuan ZHAO ; John Paul FAWCETT ; Xin XU ; Deqi CAI ; Jingkai GU
Acta Pharmaceutica Sinica B 2023;13(8):3444-3453
Irinotecan is an anticancer topoisomerase I inhibitor that acts as a prodrug of the active metabolite, SN-38. Unfortunately, the limited utility of irinotecan is attributed to its pH-dependent stability, short half-life and dose-limiting toxicity. To address this problem, a novel trivalent PEGylated prodrug (PEG-[Irinotecan]3) has been synthesized and its full-profile pharmacokinetics, antitumor activity and toxicity compared with those of irinotecan. The results show that after intravenous administration to rats, PEG-[Irinotecan]3 undergoes stepwise loss of irinotecan to form PEG-[Irinotecan]3‒x (x = 1,2) and PEG-[linker] during which time the released irinotecan undergoes conversion to SN-38. As compared with conventional irinotecan, PEG-[Irinotecan]3 displays extended release of irinotecan and efficient formation of SN-38 with significantly improved AUC and half-life. In a colorectal cancer-bearing model in nude mice, the tumor concentrations of irinotecan and SN-38 produced by PEG-[Irinotecan]3 were respectively 86.2 and 2293 times higher at 48 h than produced by irinotecan. In summary, PEG-[Irinotecan]3 displays superior pharmacokinetic characteristics and antitumor activity with lower toxicity than irinotecan. This supports the view that PEG-[Irinotecan]3 is a superior anticancer drug to irinotecan and it has entered the phase II trial stage.