OBJECTIVE:To improve the rational and scientific drug purchase quantity planned by hospital pharmacy. METH-ODS:The deficiency in present drug purchase module of hospital information system(HIS)was analyzed;the formula of planned purchase quantity was improved by incremental coefficient method,ABC analysis method and VEN analysis method. The high and low stored values and packaging volume were set to adjust planned drug purchase quantity. Based on the computer program,a one-key-getting procedure was quasi established,and five typical drugs were selected for simulation. RESULTS:Based on previous formula,the parameters representing change trend,incremental coefficient k1 and inventory coefficient k2 were added in new formu-la. The one-key-getting procedure could calculate the quantity of all drugs by simply entering the planning cycle N1 and the safe peri-od N2 of the some category drugs into the system. After simulated operation practice,compared with pre-improvement,the occupa-tion of funds was significantly decreased and the quantity of drug was rounded off according to package in post-improvement. CON-CLUSIONS:Through function improvement of drug purchase module in HIS and the quasi establishment of one-key-getting pur-chase mode,planned drug purchase quantity is more reasonable,scientific and accurate. It ensures the clinical drug supply in time without causing a backlog,and effectively reduces the cost of manpower,material and capital.

OBJECTIVE:To explore the idea and method of post-marketing safety reevaluation of TCM injection in our hospi-tal. METHODS:In case-control study,salvianolate for injection was chosen for practice. 1 000 patients with stable angina receiv-ing Salvianolate for injection in our hospital during May 2012-Oct. 2013 were selected as subjects. The occurrence of ADR/ADE were observed and Logistic regression analysis was conducted by using SPSS 20.0 software. RESULTS:The incidence of ADR/ADE was 0.6%,and rare(0.1%-1%). The ADR/ADE were general and mainly manifested as erythra(66.67%). Skin and its ap-pendants were main organs involved in ADR(66.67%). Logistic regression analysis showed that gender,drug combination and age are risk factors of ADR/ADE. CONCLUSIONS:The post-marketing safety reevaluation of TCM injection not only make up for the deficiency of pre-marketing safety evaluation of TCM injection and find out potential delayed and severe ADR/ADE and drug inter-action,but also provide reference for rational drug use in the clinic and guarantee the safe and rational drug use.

Objective:To explore the effect of mifepristone combined with methotrexate intracapsular injection on pregnancy at scar site of endogenous cesarean section.Methods:From June 2014 to June 2019, 106 cases of pregnancy at scar site of endogenous cesarean section admitted to Jinhua Maternal and Child Health Hospital were selected in the research.According to the random number table method, the patients were divided into control group and observation group, with 53 cases in each group.The control group received hysteroscopy curettage only, routine anti-infection, curettage under the guidance of B-ultrasound.The observation group was given mifepristone and methotrexate intracapsular injection on the basis of the control group.The clinical effect, recovery time of serum β-HCG, intraoperative blood loss, postoperative complications and hospitalization time were compared between the two groups.Results:The total effective rate of the observation group[92.45%(49/53)] was higher than that of the control group[75.47%(40/53)](χ 2=5.675, P<0.05). The recovery time of β human chorionic gonadotropin (β-HCG) in the observation group[(17.58±2.54)d] was faster than that in the control group[(21.83±4.13)d], and the intraoperative blood loss[(25.42±5.46)mL] was less than that in the control group[(87.93±18.47)mL], the differences were statistically significant ( t=6.381, 23.628, all P<0.05). The incidence of postoperative complications of the observation group (1.89%) was lower than that of the control group (9.43%), but there was no statistically significant difference between the two groups (χ 2=1.590, P>0.05). The hospitalization time of the observation group[(7.83±1.52)d]was shorter than that of the control group[(9.68±2.19)d]( t=5.052, P<0.05). Conclusion:Mifepristone combined with methotrexate intracapsular injection has good clinical effect on endogenous cesarean scar pregnancy.It can accelerate the recovery time of blood β-HCG, which is worthy of clinical reference.

Objective:To investigate the effect of temozolomide on autophagy of human glioma cells, and the inhibition of autophagy induced pyrocytosis on the proliferation of human glioma cells.Methods:2-64 μ mol/L of temozolomide was used to treat glioma U251 cells cultured in vitro. MTT assay was used to detect cell viability, MDC staining was used to detect autophagic vesicles in cells, cloning assay was used to detect cell proliferation, RT qPCR was used to detect the expression level of pyroptosis related mRNA in cells, Western blot was used to detect the expression of autophagy related proteins and pyroptosis related proteins in cells, and the relationship between autophagy and pyroptosis was detected by adding autophagy inhibitors.Results:Temozolamide could induce autophagy of human glioma cells, and significantly induce tumor cells to pyroptosis, thereby inhibiting the proliferation of tumor cells; RT qPCR results showed that caspase-1, GSDMD, IL-1 after temozolomide administration compared with the normal group β, the mRNA expression levels of IL-18 and NLRP3 increased significantly; Western blot results showed that Cleaved-caspase-1, Cleaved-N-terminalGSDMD, IL-1 β、IL-18 and NLRP3 protein were up-regulated; The incidence of pyroptosis decreased after the addition of autophagy inhibitors.Conclusion:Temozolamide can induce autophagy of human glioma cells, and then lead to pyroptosis, which plays an inhibitory role in proliferation.

Objective:To explore the impact of weekend hospitalization on total hospitalization expenses for elderly patients with hip fracture under the geriatric orthopedic co-management.Methods:A retrospective analysis was conducted to analyze the clinical data of elderly patients with hip fracture who had been hospitalized for surgical treatment at Beijing Jishuitan Hospital from May 2015 to December 2020. They were divided into 2 groups based on their admission date. Group A was admitted from Monday to Thursday while Group B from Friday to Sunday. The general demographic data, diagnostic information, comorbidities, hospitalization expenses of the patients were collected. The differences in total hospitalization expenses, hospitalization time, rate of surgery within 48 hours and rate of hospital mortality between the 2 groups were analyzed by rank sum test, chi square test, correlation analysis, and multiple linear regression.Results:A total of 6,075 patients with hip fracture were included in this study, including 1,675 males and 4,400 females with a median age of 80 (74, 85) years. There were 3,935 ones in group A and 2,140 ones in group B. The total hospitalization expenses for group A was 58,160.52 (49,215.45, 72,748.94) yuan, insignificantly lower than those for Group B [58,412.90 (49,163.58, 72,712.61) yuan] ( P>0.05). The rate of surgery within 48 hours for group A was 75.8% (2,984/3,935), significantly higher than that for group B [49.3% (1,054/2,140)]. The hospitalization time for group A was 5 (4, 7) days, significantly less than that for group B [5 (4, 7) days] ( P<0.05). There was no significant difference in the rate of hospital mortality between the 2 groups ( P>0.05). Multiple linear regression analysis showed that total hospitalization expenses were significantly higher for patients admitted on weekends, hospitalization time was positively correlated with total hospitalization expenses, and total hospitalization expenses were significantly lower for the patients undergoing surgery within 48 hours ( P<0.05). Conclusion:Admission on weekends can increase total hospitalization expenses, prolong hospitalization time, and reduce rate of surgery within 48 hours for elderly patients with hip fracture.

Objective:To explore the clinical characteristics of paroxysmal kinesigenic dyskinesia (PKD) secondary to pseudohypoparathyroidism (PHP), so as to improve the clinicians′ understanding of the disease.Methods:The clinical data of 2 cases of PKD secondary to PHP in Beijing Tiantan Hospital, Capital Medical University from June 2022 to October 2022 were summarized, and the related literature was reviewed to analyze the pathogenesis, clinical manifestations, laboratory examination and imaging characteristics of the disease, as well as its treatment and prognosis.Results:Ten cases of PKD secondary to PHP were previously reported. A total of 12 cases (including these 2 cases) were included in the study, of which 7 were males (7/12) and 5 were females (5/12). The onset age was 8-23 years. The clinical manifestations of the disease included paroxysmal limb torsion, limb stiffness, limb tremor, throwing movements, and dance like movements. There were 3 cases (3/12) who had seizure at the same time. Ten cases (10/12) were induced by exercise, while 2 cases (2/12) were not mentioned the inducing factor. Eleven cases (11/12) with paroxysmal symptoms lasting less than 2 minutes can be relieved automatically. Only 1 case had a family history. Laboratory examination of all patients showed low calcium, high phosphorus and significant elevation of parathyroid hormone. The head CT scans of 10 cases (10/12) showed multiple intracranial calcifications. There were 9 cases (9/12) alone received the supplementation of calcium and active vitamin D, while 3 cases (3/12) were additionally treated with antiepileptic drugs. All cases had good prognosis.Conclusions:PHP is a rare cause of secondary PKD. A small number of patients can have seizures at the same time. Most patients have a good prognosis after receiving calcium and active vitamin D supplementation.