1.Electrochemical polishing of 316L stainless steel stent
Shiwei JI ; Nan HUANG ; Guojiang WAN ; Kai WANG
Chinese Journal of Tissue Engineering Research 2011;15(16):2851-2854
Using direct current-electropolishing technique, the present study investigated the function of components and effects of operating conditions on polishing quality direct current-electropolishing of 316L stainless steel stent materials. Smooth surface was obtained quickly using this technique.
2.Effect of A Chitosan Product on Scarring and Adhesion of Injured Nerve after Laminectomy
Diya SU ; Song LIU ; Shiwei WANG ; Junhua LI ; Feng CONG ; Dongfeng LI ; Ruizhi LI ; Hong WAN
Chinese Journal of Rehabilitation Theory and Practice 2015;(1):45-48
Objective To investigate the effects of a chitosan product on scarring and adhesion after never injury in laminectomy in rats. Methods 48 male Sprague-Dawley rats were divided into group A and group B, and carried laminectomy of L4-5, and injured the right spinal nerve roots. The normal saline was smeared on the operation field in the group A, while the chitosan product was smeared in the group B. The adhesion was assessed with Rydell score 1 (groups A1/B1), 2 (groups A2/B2) and 4 (groups A3/B3) weeks after operation, while the spinal nerves were observed with HE staining and Masson staining, and the adhesion was assessed with Nussbaum score. The complete blood count and the series of blood chemistries and enzymes for liver and kidney functioning were measured. Results The Rydell scores of adhesion was I grade in group A1, II grade in A2 and III grade in A3, while was 0 grade in B1, 0 grade in B2 and II grade in B3. The scar and adhesion contact more loosely in the group B than in the group A, and the Nussbaum score was less in the group B than in the group A at the same time (P<0.05). The complete blood count and the series of blood chemistries and enzymes for liver and kidney functioning were in normal. Conclusion This chitosan product can prevent the formation of epidural scar and adhesion around the spinal and the nerve roots after laminectomy, with little toxicity and side effects.
3.Efficacy and Safety of Cinacalcet in the Treatment of Hemodialysis Patients with Secondary Hyperparathy-roidism:A Systematic Review
Shujie LI ; Shiwei RUAN ; Yuliang QIU ; Hualing LI ; Xiaojing XUE ; Yawen CHEN ; Dengpiao XIE ; Lixiang ZHANG ; Long WAN
China Pharmacy 2016;27(21):2937-2940,2941
OBJECTIVE:To systematically review the efficacy and safety of cinacalcet in the treatment of hemodialysis pa-tients with secondary hyperparathyroidism,and provide evidence-based reference for the clinical treatment. METHODS:Retrieved from Medline,Cochrane Library,EMBase and CBM,randomized controlled trials(RCT)about cinacalcet in the treatment of he-modialysis patients with secondary hyperparathyroidism (SHPT) were collected. Meta-analysis was performed by using Rev Man 5.3.5 software after data extract and quality evaluation by Cochrane systematic Rev Man 5.3.5. RESULTS:Totally 7 RCTs were en-rolled,involving 1 987 patients. Results of Meta-analysis showed cinacalcet can significantly reduce the rate of surgical parathyroid-ectomy[RR=0.23,95%CI(0.06,0.89),P=0.03],incidence of fracture[RR=0.26,95%CI(0.12,0.60),P=0.002] and increase the incidences of hypocalcemia[RR=9.81,95%CI(3.92,4.59),P<0.001],nausea[RR=1.97,95%CI(1.58,2.46),P<0.001] and vomit-ing[RR=1.91,95%CI(1.50,2.42),P<0.001],while it showed no significant effect on the the incidence of all-cause mortality and cardiovascular death. CONCLUSIONS:The clinical efficacy of cinacalcet in the treatment of hemodialysis patients with secondary hyperparathyroidism is good,but there are common adverse reactions such as nausea and vomiting,hypocalcemia.
4.Relationship between the after-school schedule and bone mineral density in middle-school students in Shanghai
FAN Jue, XU Jian,ZHANG Lina, JIANG Shiwei, LIAN Qiuyue, SUN Zhe, HU Huan, WANG Ting, WAN Xinhong
Chinese Journal of School Health 2019;40(5):676-679
Objective:
To investigate the relationship between after-school schedule and bone mineral density in middle-school students in Shanghai, to provide a reference for taking targeted measures.
Methods:
From November 2017 to April 2018,eighteen classes of six middle schools in Shanghai (from urban districts, urban-suburb combined districts and suburban districts, respectively) were selected based on cluster random sampling. In each school, 2-4 classes were further randomly chosen in the 6th grade. Questionnaires were completed by 518 students and their parents, as well as physical examination and bone mineral density assessment.
Results:
The average time spent on after-school academic learning during weekends was 4.0 (IQR: 2.0, 6.0) h. The Z-score of bone density was 0.3 (IQR: -0.7, 1.0). The smooth curve fit model showed a non-linear relationship between after-school academic learning time during weekends and the Z-scores of bone mineral densities. A two-stage multiple linear regression analysis was further applied according to the fit results, and the results showed that when total afterschool academic learning time <4.5 hours during weekends, the learning time was inversely correlated with the bone density Z-scores (β=-0.11,P=0.01), and when the learning time ≥4.5 hours, there was no significant correlation between the learning time and bone density Z-scores (β=0.02, P=0.65). Parent and student questionnaires showed that there was a non-linear relationship between students’ daily time spent on outdoor sports-related activities and bone density Z-scores. When time spent on the outdoor activities ≥45 minutes per day, outdoor activities were positively correlated with bone density Z-scores (P<0.05). However, when the time spent on outdoor activities <45 minutes per day, there was no significant relationship between outdoor activities and bone density Z-scores (P>0.05).
Conclusion
Too much time on after-school academic learning during weekends or limited time on outdoor activities are both related to impaired bone mineral densities. Therefore, a reasonable after-school schedule for middle-school students is important for physical development, especially during weekends.
5. Comparison of different styles of allogeneic hematopoietic stem cell transplantation as first-line treatment treated with severe aplastic anemia in children and adolescents
Shiwei YANG ; Rongjun MA ; Juanjuan ZHAO ; Huafang ZHONG ; Xiaoli YUAN ; Li JIANG ; Jing YANG ; Pingchong LEI ; Yin ZHANG ; Yuewen FU ; Dingming WAN ; Zunmin ZHU
Chinese Journal of Hematology 2018;39(3):184-189
Objective:
To evaluate the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) from different donors as first-line treatment for children and adolescents with severe aplastic anemia (SAA) .
Methods:
The clinical data of 79 children and adolescents with SAA diagnosed from January 2013 to December 2016 in Henan Province were retrospectively analyzed. There were 50 males and 29 females, with a median age of 14(4-18) years. 40 cases received matched sibling transplantation (MSD-HSCT), 17 with unrelated donor transplantation (UD-HSCT), and 22 with haploidentical transplantation (haplo-HSCT).
Results:
The comparison of MSD-HSCT, UD-HSCT, haplo-HSCT groups was conducted and the median times of neutrophils engraftment were statistically significant [12(9-25) d, 14(10-22) d, 16(11-26) d, respectively (
6.Effect of berberine on the expression of PGC-1α/GLUT4 in mice with insulin resistance
Shiwei WAN ; Mei YU ; Ke ZHANG ; Shiyu HAN ; Yizhi SHANG ; Penghua FANG ; Zhenwen ZHANG
Journal of Clinical Medicine in Practice 2018;22(7):7-10
Objective To observe the effect of berberine on the expression of GLUT4 and PGC-1α in mice with insulin resistance,and to explore the molecular mechanism of berberine in improving insulin resistance.Methods Mice models with insulin resistance were established,and berberine was used by intragastric administration to detect blood glucose,insulin and insulin sensitivity.RT-PCR was used to detect the expression level of GLUT4 and mRNA of PGC-1α in skeletal muscle.Results Berberine significantly reduced blood sugar,increased insulin sensitivity,and promoted the expression of GLUT4 and PGC-1α in skeletal muscle.Conclusion Berberine can improve the expression of PGC-lo,promote the expression of GLUT4 and improve insulin resistance.
7.Effect of berberine on the expression of PGC-1α/GLUT4 in mice with insulin resistance
Shiwei WAN ; Mei YU ; Ke ZHANG ; Shiyu HAN ; Yizhi SHANG ; Penghua FANG ; Zhenwen ZHANG
Journal of Clinical Medicine in Practice 2018;22(7):7-10
Objective To observe the effect of berberine on the expression of GLUT4 and PGC-1α in mice with insulin resistance,and to explore the molecular mechanism of berberine in improving insulin resistance.Methods Mice models with insulin resistance were established,and berberine was used by intragastric administration to detect blood glucose,insulin and insulin sensitivity.RT-PCR was used to detect the expression level of GLUT4 and mRNA of PGC-1α in skeletal muscle.Results Berberine significantly reduced blood sugar,increased insulin sensitivity,and promoted the expression of GLUT4 and PGC-1α in skeletal muscle.Conclusion Berberine can improve the expression of PGC-lo,promote the expression of GLUT4 and improve insulin resistance.