1.Expression of Calcitoningene related peptide in pathological tissue of congenital pseudarthrosis of tibia
Shiwei QI ; Kelai WANG ; Yan ZHUANG ; Hongyi QU ; Guoxin SONG
Basic & Clinical Medicine 2006;0(02):-
Objective To examine the expression of CGRP in congenital pseudarthrosis of tibia(CPT) in order to find the pathogenesis of CPT.Methods Periosteum and bones from CPT patients were collected as experimental group.Immunohistochemistry stain was applied to determine the differences of the expression of CGRP in two groups.Results CGRP was located at vessel wall of periosteum and intracytoplasm of osteoblasts and osteoclasts in bones,its expression was significantly less in periosteum and bones of CPT than that in control group(P
2.Effects of three-dimensional spheroid culture system on biological characteristics of mouse bone marrow mesenchymal stem cells
Xin HE ; Xue LI ; Huijing BAO ; Renfeng WANG ; Yunde LIU ; Shiwei SONG
Chinese Journal of Tissue Engineering Research 2014;(45):7227-7232
BACKGROUND:Bone marrow mesenchymal stem cel s have attracted widespread attention for the capabilities of self-renewal and muti-differentiation, which have been used in treatment of various diseases.
OBJECTIVE:To study the effect of three-dimensional spheroid culture system on the stemness and senescence of bone marrow mesenchymal stem cel s.
METHODS:Mesechyaml stem cel s were isolated from the bone marrow of C57/B6 mice, 3 weeks old, and cultured onto the culture plates coated with or without chitosan. After 5 days of culture, the cel phenotype and expression of stemness related markers CD44 and Sca-1 were analyzed by flow cytometry. PI and Annexin-V staining were used to detect cel apoptosis. Also,β-Gal staining was applied for identification of aging.
RESULTS AND CONCLUSION:The mouse mesenchymal stem cel s began to form spheroids on day 3. The stemness-related markers, including CD44 and Sca-1, expressed higher in spheroid mesenchymal stem cel s than the cel s under normal culturing. Compared with the normal culture group, the apoptosis and senescence of cel s from spheroid culture were lower. The results indicate that the formation of spheroids on chitosan films can increase the stemness, decrease the apoptosis and slow the senescence of mesenchymal stem cel s.
3.Effect of A Chitosan Product on Scarring and Adhesion of Injured Nerve after Laminectomy
Diya SU ; Song LIU ; Shiwei WANG ; Junhua LI ; Feng CONG ; Dongfeng LI ; Ruizhi LI ; Hong WAN
Chinese Journal of Rehabilitation Theory and Practice 2015;(1):45-48
Objective To investigate the effects of a chitosan product on scarring and adhesion after never injury in laminectomy in rats. Methods 48 male Sprague-Dawley rats were divided into group A and group B, and carried laminectomy of L4-5, and injured the right spinal nerve roots. The normal saline was smeared on the operation field in the group A, while the chitosan product was smeared in the group B. The adhesion was assessed with Rydell score 1 (groups A1/B1), 2 (groups A2/B2) and 4 (groups A3/B3) weeks after operation, while the spinal nerves were observed with HE staining and Masson staining, and the adhesion was assessed with Nussbaum score. The complete blood count and the series of blood chemistries and enzymes for liver and kidney functioning were measured. Results The Rydell scores of adhesion was I grade in group A1, II grade in A2 and III grade in A3, while was 0 grade in B1, 0 grade in B2 and II grade in B3. The scar and adhesion contact more loosely in the group B than in the group A, and the Nussbaum score was less in the group B than in the group A at the same time (P<0.05). The complete blood count and the series of blood chemistries and enzymes for liver and kidney functioning were in normal. Conclusion This chitosan product can prevent the formation of epidural scar and adhesion around the spinal and the nerve roots after laminectomy, with little toxicity and side effects.
4.Primary and Secondary Somatosensory Cortex Activation Induced by Mild and Moderate Pain: A Functional Magnetic ResonanceImaging Study
Weiyi GONG ; Yunjing XUE ; Shiwei SONG ; Liangcheng ZHANG ; Jiansheng SU ; Chongjiu FAN
Chinese Journal of Rehabilitation Theory and Practice 2012;18(5):423-425
Objective To explore the activation of the primary somatosensory cortex (SⅠ) and secondary somatosensory cortex (SⅡ) undermild and moderate pain. Methods 7 healthy right-handed volunteers accepted electrostimulation of 1 and 2 times pain threshold respectively.The severity of pain was determined with the Visual Analogue Score (VAS), and they received functional magnetic resonance imaging(fMRI) simultaneously. Results 1 and 2 times pain threshold electrical stimulation caused mild and moderate pain respectively. Bothmild and moderate pain activated contralateral SⅠ similarly, and bilateral SⅡ with pain intensity. Conclusion SⅠ and SⅡ respond to the mildand moderate pain in different ways.
5.Laplacian-Regularized Mean Apparent Propagator-MRI in Evaluating Corticospinal Tract Injury in Patients with Brain Glioma
Rifeng JIANG ; Shaofan JIANG ; Shiwei SONG ; Xiaoqiang WEI ; Kaiji DENG ; Zhongshuai ZHANG ; Yunjing XUE
Korean Journal of Radiology 2021;22(5):759-769
Objective:
To evaluate the application of laplacian-regularized mean apparent propagator (MAPL)-MRI to brain glioma-induced corticospinal tract (CST) injury.
Materials and Methods:
This study included 20 patients with glioma adjacent to the CST pathway who had undergone structural and diffusion MRI. The entire CSTs of the affected and healthy sides were reconstructed, and the peritumoral CSTs were manually segmented. The morphological characteristics of the CST (track number, average length, volume, displacement of the affected CST) were examined and the diffusion parameter values, including fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), radial diffusivity (RD), mean squared displacement (MSD), q-space inverse variance (QIV), returnto-origin probability (RTOP), return-to-axis probabilities (RTAP), and return-to-plane probabilities (RTPP) along the entire and peritumoral CSTs, were calculated. The entire and peritumoral CST characteristics of the affected and healthy sides as well as those relative CST characteristics of the patients with motor weakness and normal motor function were compared.
Results:
The track number, volume, MD, RD, MSD, QIV, RTAP, RTOP, and RTPP of the entire and peritumoral CSTs changed significantly for the affected side, whereas the AD and FA changed significantly only in the peritumoral CST (p < 0.05). In patients with motor weakness, the relative MSD of the entire CST, QIV of the entire and peritumoral CSTs, and the AD, MD, RD of the peritumoral CST were significantly higher, whereas the RTPP of the entire and peritumoral CSTs and the RTOP of the peritumoral CST were significantly lower than those in patients with normal motor function (p < 0.05 for all). In contrast, no significant changes were found in the CST morphological characteristics, FA, or RTAP (p > 0.05 for all).
Conclusion
MAPL-MRI is an effective approach for evaluating microstructural changes after CST injury. Its sensitivity may improve when using the peritumoral CST features.
6.Laplacian-Regularized Mean Apparent Propagator-MRI in Evaluating Corticospinal Tract Injury in Patients with Brain Glioma
Rifeng JIANG ; Shaofan JIANG ; Shiwei SONG ; Xiaoqiang WEI ; Kaiji DENG ; Zhongshuai ZHANG ; Yunjing XUE
Korean Journal of Radiology 2021;22(5):759-769
Objective:
To evaluate the application of laplacian-regularized mean apparent propagator (MAPL)-MRI to brain glioma-induced corticospinal tract (CST) injury.
Materials and Methods:
This study included 20 patients with glioma adjacent to the CST pathway who had undergone structural and diffusion MRI. The entire CSTs of the affected and healthy sides were reconstructed, and the peritumoral CSTs were manually segmented. The morphological characteristics of the CST (track number, average length, volume, displacement of the affected CST) were examined and the diffusion parameter values, including fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), radial diffusivity (RD), mean squared displacement (MSD), q-space inverse variance (QIV), returnto-origin probability (RTOP), return-to-axis probabilities (RTAP), and return-to-plane probabilities (RTPP) along the entire and peritumoral CSTs, were calculated. The entire and peritumoral CST characteristics of the affected and healthy sides as well as those relative CST characteristics of the patients with motor weakness and normal motor function were compared.
Results:
The track number, volume, MD, RD, MSD, QIV, RTAP, RTOP, and RTPP of the entire and peritumoral CSTs changed significantly for the affected side, whereas the AD and FA changed significantly only in the peritumoral CST (p < 0.05). In patients with motor weakness, the relative MSD of the entire CST, QIV of the entire and peritumoral CSTs, and the AD, MD, RD of the peritumoral CST were significantly higher, whereas the RTPP of the entire and peritumoral CSTs and the RTOP of the peritumoral CST were significantly lower than those in patients with normal motor function (p < 0.05 for all). In contrast, no significant changes were found in the CST morphological characteristics, FA, or RTAP (p > 0.05 for all).
Conclusion
MAPL-MRI is an effective approach for evaluating microstructural changes after CST injury. Its sensitivity may improve when using the peritumoral CST features.
7.Bladder cancer incidence trend:the analysis of Shenzhen in 2003-2012
Shiwei YANG ; Song WU ; Ji PENG ; Lin LEI ; Yi SONG ; Yiyue FANG ; Huiling JIANG ; Zhiming CAI
Chinese Journal of Modern Nursing 2015;(32):3861-3863,3864
Objective To describe the prevalence and characteristics of bladder carcinoma of Shenzhen residents in recent 10 years and make a relative comparison of corresponding data so as to provide basis for the prophylaxis and treatment of Shenzhen. Methods We collected the data of bladder cancer from 2003 to 2012 in Shenzhen and made a statistical analysis of gender, age, crude incidence rate,standardized incidence and other indexes in China of permanent population and registered population in Shenzhen, then we compared the incidence the trends of the two groups. Results Bladder cancer incidence in Shenzhen for the past decade was as follows. For the permanent residents, the incidence in male and female and total incidence of the disease were 2. 37/105 , 0. 75/105 and 1. 63/105 respectively; for the population of male, the incidence in male and female and total incidence of the disease were 4. 77/105 , 1. 19/105 , 3. 09/105 respectively;standardized incidence in China were 17. 1/105 , 4. 53/105 , 11. 21/105 respectively; all of which were higher than the national average level. Conclusions The registered population of Shenzhen has a higher bladder cancer incidence that even reaches the level of developed countries. Therefore, the task of prophylaxis and treatment are arduous.
8.A clinical observation of cool-tip radiofrequency ablation assisted enucleation for giant renal angiomyolipoma.
Shiwei ZHANG ; Lijin ZHANG ; Xiaozhi ZHAO ; Changwei JI ; Guangxiang LIU ; Xiaogong LI ; Jiannan SONG ; Hongqian GUO
Chinese Journal of Surgery 2016;54(2):129-132
OBJECTIVETo initially explore the clinical effect of cool-tip radiofrequency ablation combined with enucleation for the giant hamartoma of kidney with narrow base and export-oriented way of growth.
METHODSThe clinical date of 15 patients including 6 male and 9 female with special hamartoma of kidney underwent cool-tip radiofrequency ablation assisted enucleation from July 2011 to October 2014 were reviewed.The median age was 49 years (ranging from 35 to 71 years). There were 6 cases with left renal tumor, 8 cases with right renal tumor and 1 case with solitary kidney tumor.All patients were confirmed by B ultrasound or CT scan, the mean diameter of hamartoma of kidney was 9.7 cm(8.5-12.7)cm, all tumors were located distant from the collecting system and presents with a special way of growth.The preoperative hemoglobin was (129±18)g/L, SCr was (92±41)μmol/L, glomerular filtration rate (GFR) was (32±12)ml·min(-1)·1.73 m(-2).
RESULTSCool-tip radiofrequency ablation assisted enucleation was technically successful in all patients.The mean operative duration was (115±31)minutes, and the average intraoperative bleeding was (72±21)ml with no blooding transfusion.The postoperative hospital stay was(7±2)days, and the postoperative hemoglobin was(129±18)g/L, SCr (92±41)μmol/L, GFR(30±15)ml·min(-1)·1.73 m(-2). No statistic change of hemoglobin and SCr or glomerular filtration rate after operations(all P>0.05). Postoperative pathology showed that all cases were hamartoma of kidney.During a mean follow-up period of 19.5 months, none of them had local tumor recurrence or chronic renal insufficiency.
CONCLUSIONSCool-tip radiofrequency ablation assisted enucleation is both safe and effective in the treatment of huge hamartoma of kidney with a narrow base and export-oriented way of growth. The short-term follow-up shows a satisfactory therapeutic effect.
Adult ; Aged ; Angiomyolipoma ; surgery ; Blood Transfusion ; Catheter Ablation ; methods ; Female ; Glomerular Filtration Rate ; Humans ; Kidney Neoplasms ; surgery ; Length of Stay ; Lipoma ; therapy ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Postoperative Period ; Treatment Outcome
9. Analysis of prognostic factors for hyperamylasemia following pancreaticoduodenectomy
Huan WANG ; Zhuo SHAO ; Shiwei GUO ; Wei JING ; Bin SONG ; Gang LI ; Tianlin HE ; Xuyu ZHOU ; Yijie ZHANG ; Yingqi ZHOU ; Xiangui HU ; Gang JIN
Chinese Journal of Surgery 2019;57(7):534-539
Objective:
To investigate the prognostic factors of hyperamylasemia following pancreaticoduodenectomy (PD) .
Methods:
Clinical data of 359 patients were collected prospectively who underwent PD by the same group at Changhai Hospital of Navy Medical University from January 2017 to June 2018.There were 212 males and 147 females.The median age was 63 years old (range: 23 to 82 years old) .According to whether the patient′s serum amylase was greater than 120 U/L at 0 or 1 day after surgery,the patients were divided into hyperamylasemia group and non-hyperamylasemia group. Univariate analysis and multivariate analysis were used to find out the prognostic factors of hyperamylasemia after PD.
Results:
Of the 359 patients, 238 cases (66.3%) developed hyperamylasemia.The incidence rate of clinically related pancreatic fistula (15.1%
10.Effect of complete nursing order guidelines on improving the nursing quality in ICU
Mei YANG ; Haiyan MO ; Yi SONG ; Mingqu LI ; Haiying RUAN ; Shiwei YANG
Chinese Journal of Modern Nursing 2014;20(20):2561-2564
Objective To discuss the effect of the complete nursing order and guidelines on improving the nursing quality in ICU .Methods The nursing quality , patients ’ satisfaction and the hierarchical training examination scores before and after using the improved nursing order guidelines were compared , from 2012 to 2013 .Totals of 177 patients in 2012 were treated with conventional nursing order system , and 183 patients in 2013 were treated with the improved nursing order guidelines .Results In 2012, totals of 177 patients with 3 566 times nursing observation were included , and 183 patients, with 3 634 times nursing observation were included in 2013.In 2013, oral care failure rate was 0.28%, which was significantly lower than 1.18% in 2012, with a significant difference(χ2 =20.45, P=0.00).In 2013, the bed elevation unqualified rate was 0.33%, which was significantly lower than 1.54% in 2012, with a significant difference (χ2 =28.69,P=0.00).In 2013, the artificial airway ballonet pressure implementation rate was 99.56%,which was significantly higher than 98. 65%in 2012, with a significant difference (χ2 =16.76,P=0.00).In 2013, the patients’ satisfaction scores was (98.08 ±0.67), which was significantly higher than (94.58 ±2.47) in 2012, with a significant difference (t=4.91,P =0.00).The nurse training assessment scores was (89.33 ±9.64) in 2013, which was significantly higher than ( 82.17 ±4.04 ), with a significant difference ( t =2.62, P =0.02 ). Conclusions Nursing order guidelines applied in ICU bedside can promote the improvement of the nursing quality, ensure the safety of nursing care , and let patients receive continuous and homogeneous care .Improved nursing order guidelines not only effectively improve the whole nursing level of ICU , but also provide diversified platform for nurse training at all levels .