Objective To explore the influence of rs1360780 T risk allele of FK506-binding protein 5 (FKBP5) gene on the brain function under resting-state and its association with clinical symptoms as well as immune function in patients with major depressive disorder (MDD).Methods Totally 147 MDD patients and 61 gender-,age-,and education-matched healthy controls were scanned with 3.0T MRI Scanner and genotyped.The peripheral serum immunoglobulin and complement were measured.The main effect of the disease,the genotype and their interaction effects were analyzed using regional homogeneity (ReHo) by two-way ANOVA.Abnormal brain activity was identified in T risk allele carriers of rs1360780 and non-risk CC individuals in MDD using post hoc analyses.Correlation analyses were performed between ReHo values of significant brain regions and the total score,five-factor scores of Hamilton rating scale for depression (HAMD-17),serum levels of immunoglobulin and plasma complement component in MDD patients.Results (1) The results of 2x 2 ANOVA showed the interaction effects located in the left opercular part of inferior frontal gyrus (MNI:x,y,z =-42,6,9;F=10.83),right opercular part of inferior frontal gyrus (MNI:x,y,z =30,6,33;F=15.05),left medial superior frontal gyrus (MNI:x,y,z=-9,54,0;F=9.17) and left pallidum (MNI:x,y,z =-12,6,-6;F=11.37) (Alphasim corrected,P＜ 0.05).(2) In post-hoc analyses for the main effect of genotype,T+ carriers with MDD showed increased ReHo values in the right opercular part of inferior frontal gyrus (MNI:x,y,z=60,12,6;t=2.88) compared with CC carriers;for the effect of diseaseby-genotype interaction,T+ carriers with MDD showed increased ReHo values in the right opercular part of inferior frontal gyrus (MNI:x,y,z=30,6,33;t=2.96) and decreased ReHo values in the left orbital part of inferior frontal gyrus (MNI:x,y,z =-21,9,-18;t =-3.21) (Alphasim corrected,P＜ 0.05) in contrast to CC carriers.(3)Pearson's correlation showed that the average ReHo values of the right opercular part of inferior frontal gyrus negatively correlated with the content of immunoglobulin G (r=-0.528,P=O.0016,Bonferroni corrected) and positively correlated with anxiety/somatization factor score (r=0.421,P＜0.001,Bonferroni corrected) in T + carrìers with MDD.Conclusion The results of this study suggest that rs1360780 T-risk allele of FKBP5 gene is involved in the changes of local neural activity in the right opercular part of inferior frontal gyrus of depressed patients and could potentially indicate a neuropathological mechanism of anxiety somatic symptoms and immune dysfunction in depression.

Objective:To explore the difference of amplitude of low frequency fluctuation(ALFF) in amygdala subregions between anxious depression patients and non-anxious depression patients, and its correlation with clinical features.Methods:A total of 144 patients with depression diagnosed by DSM-Ⅳ-TR criteria and exclusion criteria in Nanjing Brain Hospital, meanwhile 62 healthy controls with matching demographic characteristic were recruited.All subjects were scanned with 3.0 T fMRI scanner.The severity of the patients was assessed by Hamilton depression scale (HAMD-17). After preprocessing magnetic resonance data with DPARSFA toolkit in Matlab, ALFF values of each subregion of amygdala were calculated with rest toolkit for different groups of subjects.Patients were divided into anxious group ( n=65) and non-anxious group ( n=79) according to whether their anxiety/somatization factor score was greater than 7.In SPSS 19.0, ANOVA was used to calculate the difference of ALFF in amygdala subregion in three groups, then two-sample t test was used to find the differences between each other group (Bonferroni multiple comparison correction, P<0.05). Pearson correlation analysis was performed between ALFF values of brain regions with significant differences and clinical factors. Results:Compared with the non-anxious group(left central amygdala: 1.12±0.21, left dorsolateral nucleus: 1.01±0.26, left subcortical amygdala: 1.49±0.46), the ALFF values of the left central amygdala(1.22±0.18), left dorsolateral nucleus(1.16±0.33) and left subcortical amygdala(1.90±0.66) in anxious depression group were significantly different (all P<0.01, Bonferroni corrected). Correlation analysis showed that the ALFF value of the left central amygdala in patients with anxious depression was positively correlated with the anxiety/somatization factor score ( r=0.473, P=0.008). Conclusion:Abnormal left amygdala function in patients with depression may be the neuropathological basis of anxiety, among which the abnormal left central nucleus brain area may be related to the severity of anxiety/somatization.