1.One case report of carotid body tumor resected with hemorrhage and artery repair.
Shuichao GAO ; Youzhong LI ; Shisheng LI
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(2):101-102
Carotid body tumors (CBT) were rare. One case with a mass in the right neck side suspected as CBT after preoperative examination was reported with hemorrhage, and artery repair in the resection of the mass, which was finally diagnosed as CBT by pathological examination. Its resection methods, postoperative complications and artery repair methods were discussed.
Adult
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Arteries
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surgery
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Blood Loss, Surgical
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Carotid Body Tumor
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surgery
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Humans
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Male
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Reconstructive Surgical Procedures
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methods
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Treatment Outcome
2.The adipocytokine levels and their association with insulin resistance among patients with type 2 diabetes mellitus and impaired glucose regulation in Guangxi Province
Cuijun PANG ; Changqing XIAO ; Shisheng LI
Chinese Journal of Diabetes 2005;13(5):326-327
In the study of T2DM(n=30),IGR(n=26) and NGT(n=30) cases in Guangxi Province,T2DM had higher resistin and PAI-1 levels than did the groups of IGR and NGT(P<0.05).IGR had higher levels of PAI-1 and leptin than NGT(P<0.05).Leptin level of T2DM was higher than that of NGT(P<0.05).Insulin resistance index was positively correlated with leptin,TG and PAI-1 levels(P<0.05).
3.Effect of long needle penetration needling on matrix metalloproteinase inhibitor 1 in the synovium of knee osteoarthritis model rats
Shisheng LI ; Yaochi WU ; Junfeng ZHANG
Chinese Journal of Tissue Engineering Research 2016;20(18):2609-2614
BACKGROUND: Matrix metal oproteinase inhibitor 1 plays an important role in the inhibition of articular cartilage degeneration and destruction.
OBJECTIVE: To observe the changes of matrix metal oproteinase inhibitor 1 expression level in synovium of knee osteoarthritis rats after treated with penetration needling of long needle.
METHODS: A total of 40 Sprague-Dawley rats were randomly assigned into normal group, penetration needling group, drug group and model group, with 10 rats in every group. 1.6% papain solution was injected into knee joint cavity of Sprague-Dawley rats. 2 weeks later, knee osteoarthritis models were established. Rats in the normal and model groups did not receive any intervention. In the penetration needling group, rats were treated by penetration needling with 0.3 mm×125.0 mm filiform needle. Rats in the drug group were intraarticularly injected with sodium hyaluronate.
RESULTS AND CONCLUSION: (1) Enzyme linked immunosorbent assay: at 4 weeks after treatment, matrix metal oproteinase inhibitor 1 expression in synovial membrane was higher in the normal group than in the model group (P < 0.05). Matrix metal oproteinase inhibitor 1 expression in synovial membrane was higher in the penetration needling group and drug group than in the model group (P < 0.05). (2) Results verified that long needle penetration therapy can effectively increase matrix metal oproteinase inhibitor 1 expression in synovial membrane of knee osteoarthritis model rats.
4.Clinical Study on Treatment of Knee Osteoarthritis with Combined Acupuncture and Medication
Chongmiao WANG ; Shisheng LI ; Chouping HAN
Journal of Acupuncture and Tuina Science 2009;7(6):354-356
Objective: To observe the effect of treatment for proliferative knee osteoarthritis (KOA) using combined acupuncture and medicated compression. Method: All 328 cases were randomized into an observation and control group, 164 cases in each. Combined acupuncture and medicated compression were adopted in the observation group, while oral administration of Zhui Feng Tou Gu Wan (Wind-removing and Bone-penetrating Pills) were adopted in the control group. The clinical effects in the two groups were evaluated 40 days after the treatment. Result: There were significant differences in both marked and total effective rates (P<0.05). Conclusion: Combined acupuncture and medicated compression can obtain better effect for KOA than oral administration of the Wind-Removing and Bone-Penetrating pills.
5.Minor Phenolic Constituents of Chinaberry-tree (Melia azedarach)
Shisheng LI ; Jingzhen DENG ; Shouxun ZHAO
Chinese Traditional and Herbal Drugs 2000;31(2):86-89
Five minor ferulic acid esters (Ⅰ~Ⅴ) were identified in a petrol extract of Melia azedarach stem bark, one (Ⅰ) of which was isolated with no impurity for the first time. By chemical and spectral methods, their structures were elucidated as hexacosylferulate (Ⅰ); tetracosylferulate (Ⅱ); pentacosylferulate (Ⅲ); heptacosylferulate (Ⅳ) and octacosylferulate (Ⅴ), respectively.
6.Sexual dysfunction in men with spinal fractures complicated by incomplete paraparesis
Shisheng HE ; Tiesheng HOU ; Ming LI
Chinese Journal of Trauma 1993;0(05):-
Objective To review and analyze the sexual function of patients with spinal fractures complicated by incomplete paraparesis. Methods A total of 73 patients with incomplete paraparesis resulted from spinal fractures were retrospectively analyzed. Frankel Grade was used to determine the neurologic function and international index of ecrectile function (IIEF) to evaluate patients' sexual function. The patients with sexual dysfunction also were examined for reflex erection and psychogenic erection function. Results There were 21 patients with sexual dysfunction. Through ?2 test, significant difference was found between Frankel grade B and grade C as well as between grade D and grade E (P
7.The vitro research of effects of Beclin1 on paclitaxel-sensitivity in laryngeal carcinoma cell Hep-2.
Xiaocong DENG ; Xinming YANG ; Shisheng LI
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(2):159-163
OBJECTIVE:
Background: We detect the effects of Beclinl on paclitaxel-sensitivity in laryngeal carcinoma cell.
METHOD:
This study used Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl as invitro model. The effect of paclitaxel on the proliferation and cell apoptosis of laryngeal cancer cell lines was evaluated by MTT assay and flow cytometry. The protein expression level of Akt and p-Akt was detected by Western blot. Result: After treated by paclitaxel, the inhibition rate was significantly higher in Hep-2-Beclin cells than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<. 05). After dealing with 10 tg/L paclitaxel, the apoptosis rate in Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl were (23. 75 ± 2 3. 77) %, (21. 25 ± 4. 92) %, (32. 50 ± 5. 97) %, respectively. After dealing with 20µg/L paclitaxel, the apoptosis rate in Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl were (38. 75 ± 4. 79) %, (38. 75±6. 55) %, (50. 00±7. 26) %, respectively. Paclitaxel-induced apoptosis was higher in Hep-2-Beclin cells than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<. 05). The result of western blot showed that the protein expression level of p-Akt in Hep-2-Beclin cells was lower than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<0. 05) and the protein expression level of Akt was similar in three cell lines (P>0. 05).
CONCLUSION
Beclinl enhances paclitaxel-sensitivity by inhibition of PI3K/Akt pathway.
Apoptosis
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Apoptosis Regulatory Proteins
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physiology
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Beclin-1
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Cell Line, Tumor
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Cell Proliferation
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Humans
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Laryngeal Neoplasms
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pathology
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Larynx
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Membrane Proteins
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physiology
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Paclitaxel
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pharmacology
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Phosphatidylinositol 3-Kinases
8.Clinical value of bronchoscopy in dignosis of bacteriological negative pulmonary tuberculosis
Ruixia ZHANG ; Xiaohong LI ; Shisheng ZENG
Chinese Journal of Primary Medicine and Pharmacy 2013;20(14):2142-2144,后插2
Objective To explore the diagnostic value of bronchoscopy combined materials drawn in multiply methods in patients with bacteriological negative pulmonary tuberculosis.Methods The clinical data of 74 patients with pulmonary tuberculosis were retrospectively analyzed.All the patients had sputum acid-fast staining more than three times,25 patients were sputum bacteriological positive and 49 patients were bacteriological negative or had no sputum.Bronchoscopy was performd in 28 cases,including biopsy examining,brush examining,bronchoalveolar lavage fluid(BALF) smear and sputum spear acid-fast stain test.Results In 28 patients undergoing examination,microscopic abnormalities were found in 14 cases,taken 50.0%.15 cases performed lesion biopsy or transbronchiallung biopsy(TBLB) were diagnosed tuberculosis,taken 53.6%.Brush acid-fast staining was positive in 7 cases,taken 25.0%.BALF smear postive in 10 cases,taken 35.7%.And phlegm test after bronchoscope positive in 6 cases,taken 21.4%.Combined multiply index,23 cases finally diagnosed as pulmonary tuberculosis,taken 82.1%.Conclusion Bronchoscopy with materials drawn in multiply methods has a high value in diagnosing bacteriological negative pulmonary tuberculosis,we should take advantage of it as soon as possible.
9.Therapeutic Observation on Tuina plus Electroacupuncture for Lateral Humeral Epicondylitis
Shisheng LI ; Yaochi WU ; Junfeng ZHANG ; Jinghui ZHOU
Journal of Acupuncture and Tuina Science 2014;(5):321-325
Objective: To observe the clinical efficacy of tuina combined with electroacupuncture (EA) in treating lateral humeral epicondylitis. <br> Methods: Totally 118 patients were randomly allocated into a treatment group and a control group, 59 cases in each. The patients in the treatment group were treated with tuina combined with EA treatment, while those in the control group were treated by EA alone. Clinical efficacies of the two groups were compared after the treatment. <br> Results: The total effective rate was 93.2% in the treatment group, versus 84.7% in the control group, and the difference was statistically significant (P<0.05). <br> Conclusion: Tuina combined with EA treatment for lateral humeral epicondylitis has a significant effect.
10.Clinical Observation on Treatment of Knee Osteoarthritis by Warm Needling Method
Yaochi WU ; Junfeng ZHANG ; Shisheng LI ; Guoqi HUANG
Journal of Acupuncture and Tuina Science 2009;7(6):349-351
Objective: To observe the therapeutic effect of warm needling method for knee osteoarthritis. Methods: Three hundred cases were randomly divided into treatment group and control group, 150 cases respectively in each group. The treatment group was treated by warm needling method, and the control group was treated by simple acupuncture. Their therapeutic effects were compared after 20 sessions of treatments. Results: The effective rate was 94.0% in the treatment group and 86.0% in the control group, with a significant difference between the two groups (P<0.05). Conclusion: The therapeutic effect is better in the warm needling group than in the control group in the treatment of knee osteoarthritis.