Objective To study the nation-wide cooperation network of bio-genomics for major disease clinical samples and the development of bio-genomics database.Methods The clinical phenotypes and genomics data of disease cohort were investigated,the cooperation network of bio-genomics for major disease clinical samples and the development of bio-genomics database were analyzed,the key technologies for different tasks were studied.Results The key contents and technologies involving the development of bio-genomics database for major disease clinical samples were proposed and how to solve the following problems was pointed out,including the integration of phenotypes and new technologies in the genomics field,multi-granular and multi-modular retrieval,and computing service.Conclusion It is of great importance to establish the standards for data cooperation process and data security management,to research and develop platform for cooperation network and database,to study the service model for the integration of big data in precision medicine based on data cooperation network.

In the process of development and exploitation of information resources for the medicalsector, business synergy and information exchange/sharing become more and more frequent across departments. How to effectively sort massive information resources involved in the departments has become a bottleneck. The author proposed a complete set of thoughts to sort relations among businesses, from the aspects of functionality layer, service layer, and data layer, in view of the business problems to resolve by the departments.

Objective To study the retrieved human allografts pathologically in order to probe into the histological changes of implanted allograft. Methods The specimens were retrieved from seven cases between February 2003 and March 2004, and the allografts had been in situ from two to 16 months. Of seven cases, four were diagnosed primarily as malignant fibrohistocytoma of proximal humerus, osteosarcoma of femoral shaft, bone giant cell tumor of distal femur and bone giant cell tumor of proximal tibia each, which were treated with wide resection of tumor and massive allograft transplantation and internal fixation of plate or inter-locking nail; two were open comminuted fracture of distal femur, treated with massive allograft transplantation and internal fixation, underwent autograft bone transplantation because of united massive allograft, had a punch biopsy of allografts for observation; one was tibial open fracture being fixated with inter-locking nail and small segment allograft transplantation, sustained infection at two weeks after primary operation and underwent debriment, removal of internal fixation and external fixation. Results Of seven cases, five were found with bone absorption radiographically, and were classified into three types:1)sever bone absorption; 2)bone absorption associated with sinus and effusion of the wound; 3)bone absorption accompanied by pyogenic infection. The later condition was caused by bacterial infection without a relationship of allograft. And the former two conditions could be classified into two subtypes: 1)osteoclastic absorption, which was followed by new bone formation; 2)inflammatory absorption, which was found with infiltration by round inflammatory cell, preponderantly lymphocytes, and mononuclear phagocytes with few osteoclasts, osteoblasts or new bone formations. Moreover, in sever bone absorption, vascular lesions such as vasculitis, swelling of endothelial cells or proliferation changes in the vessels, and obliteration of the arteries. Conclusion An intensive infiltration accompanied by vascular lesions may be the evidences of histological changes suggestive of an immune reaction directed against the grafts, and the failure of the clinical outcomes.

The assessment method for pharmacology courses in top-up program of Yanjing medical college was reformed supported by the principal foundation from China capital medical university.The reform specified the assessment content and examination syllabus,formed the multilayer assessing methods and assessing modes and assessed objectively through questionnaires and investigations.The results showed that the students were satisfied with the assessment mode,degree of standardization and the implementation effect. Pharmacology teaching was effectively assessed by the new method and students′ comprehensive ability was improved.

Objective To clone and analyze the MN/CA9 gene sequence in ICR mice and detect the expressions of MN/CA9 gene in various tissues of ICR mice.Methods The fresh tissues of small intesine, uterrus, skin, musle, liver, pancreas, heart, lung, thymus, spleen, kidney, ovary, stomach ,urinary bladder from ICR mice were obtained , the total RNA was extracted by GIT method, the 1st strand and 2nd strand of cDNA were synthesized, the EcoRⅠ adapters were lingated,the EcoRⅠ ends were phosphorylated, digested with XhoⅠ ,cDNA was ligated into the ZAP expression vector, packaged, planted, screened.The expressions of MN/CA 9 gene in various tissues in mice were detected by RT-PCR.Results A fragment of human MN/CA9 gene was used as probe, and 1.47?10~3 clones were screened with radioactive isotopic ~ 32 P labeled probe, after hybridizations, one positive signal of cDNA was detected and the complete nucleotide sequence contained 1 671 bp was determined (GenBank:AB086322), The nucleotide similarity between mouse and human cDNA (GenBank:Z54349) was 69.1%.The MN/CA9 gene detected by RT-PCR assay (primer: P521-P1193) strongly expressed in small intesine,uterus, musle, pancreas, heart, lung, thymus, spleen, kidney, ovary, stomach,and urinary bladder,meanwhile did not express in skin and liver. Conclusion The expressions of MN/CA9 gene in some tissues of ICR mice are similar to that of human, it can be used to further functional analysis of MN/CA9 gene.

Objective:To study the application and clinical significance of autotransfusion hemodilution inlaryngectomy. Method:Autotransfusion hemodilution was adopted in 30 patients with Ⅲ or Ⅳ stage laryngealcarcinoma during operation as the experimental group, and the blood transfusion only was adopted in the patientswith excessively blood loss. The other 30 patients with the same stages of disease and the same kinds ofoperation were performed as the control group, without autotransfusion hemodilution and the blood transfusionwas adopted if necessary. Result: Blood transfusion was necessary to be performed only in 8 patients of theexperimental group, on the contrary,it was necessary in 19 of 30 patients of the control group (P＜0.01).There were no complications during the operations , and the postoperative complications were no significance (P＞0. 05) between two groups. Conclusion:The application of autotransfusion hemodilution may be of help to aplenty of patients with laryngeal carcinoma in advanced stage to keep off the blood transfusion duringlaryngectomy.

Medical big data, an important strategic source of basic data for a country, will be applied in precision clinical diagnosis and treatment, decision-making support, disease monitoring, early warning and management, and public health service. The application of medical big data technology in our country is to be improved at pres-ent. How to realize the smooth transition of traditional medical data to a big data system and the added value of data by data mining and analyzing is an important problem needing to be solved immediately. The key functions, inclu-ding the general frame work and data center frame work of medical big data application information system, were thus planned and designed in this paper by constructing the regional application technology and engineering labora-tory for medical big data.

A nutrition survey, covering a total of 4801 preschool children in 6 provinces, autonomous regions and municipalities, has been carried out. The objective was to determine the effects of various dietary patterns on the blood hemoglobin, free erythrocyte proporphyrin, and serum ferritin values. The results indicated a high prevalence of iron deficiency (average 51.9%). Iron deficiency anemia was detected in 16.4% of the children examined and the incidence rate was particularly high among infants at the age group of 6 months and one year, which was 28.7% and 22.9% respectively. The dietary patterns were different in the above geographic areas. The results of dietary survey revealed that a low incidence of iron deficiency was found in those children who consumed more animal food and protein.

Objective: To evaluate the physical properties of SY-1 silicone elastomer with various ratios of cross-linker to base. Methods: The ratios of cross-linker to base were set at 0.5 %、1 %、 1. 5 %、 2 % 、2. 5 % respectively while other conditions were kept same, then the major physical properties of the elatomer were studied with a tensile tester and ASTMD 2240 method. Results: The elongation(%) due to tension, tensile strength (MPa), laceration strength (kN/m) and hardness (A) of SY-1 silicone elastomer with the ration of 0. 5% of cross-linker to base were 540?47,4. 3?0. 28, 14. 4?2.4 and 18. 0?3. w; those of that with 1% 440 ?54,4.0?0.32,18. 2?3.1 and 24. 0?3. 61 with 1.5% 430?51, 4.1?0. 29, 13.6?2.2 and 25.0?2.1; with 2% 350?46, 2.5?0. 33, 9.0?2.1 and 28.0?1.9; with 2.5% 280?36, 2.0?0. 3, 11.0?2.5 and 29.0? 2.8, respectively. Conclusion: The physical properties of SY-1 silicone elastomer with a ratio of 1% of crosslinker to base are more suitable for prothesis fabrication.

Objective To investigate effects of hyperbaric oxygen (HBO) therapy on C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) levels in patients with moderate or severe traumatic brain injury (TBI)and to analyze its therapeutic efficacy. Methods One hundred and eight patients with moderate or severe TBI were randomly divided into a control group (54 cases) and an HBO adjunctive therapy group (HBO group, 54 cases).Both groups received essential neurosurgical treatment and conventional drug treatment, and the HBO group was given one session of HBO therapy in addition. Serum CRP and TNF-α were detected, and the scores on the Glasgow coma scale (GCS) were measured before and after treatment. CRP was detected by turbidimetric immunoassay and TNF-α using ELISA. Glasgow outcome scale (GOS) scores were evaluated in a follow-up 6 months after injury. Results Average CRP, TNF-α and GCS measurements showed no statistically significant difference between the groups before treatment. After treatment, CRP and TNF-α were significantly lower and GCS scores significantly better in both groups, but patients in the HBO group were, on average, significantly better than the controls on all three measures.Six months later, GOS evaluation gave a significantly larger number of patients with a better prognosis in the HBO group compared with the controls. Conclusion HBO therapy can significantly decrease serum CRP and TNF-α after severe TBI, thus enhancing therapeutic efficacy.