Objective To investigate the effect of Hyaluronic acid(HA) on the expression of matrix metalloproteinases(MMPs)-3,9,13 and tissue inhibitor of MMPs(TIMP)-1 mRNA stimulated by interleukin (IL)-1β in chondrocytes from arthritis model in vitro.Methods Different levels of Sodium Hyaluronate(10,20,40μg/ml)were added to culture medium of rat chondrocytes.The ehondrocytes were isolated from rat osteoarthritis model.One hour later,10 ng/ml IL-1β were added to each sample and co-cultured for 24 hours.The expressions of MMP-3,9,13 and TIMP-1 mRNA were detected bv RT-PCR.The concentration of nitric oxide (NO) in the supernatants were measured by Griess reaction.Results HA could down-regulate MMP-3,9,13 mRNA expression but had no effect on TIMP-1 mRNA expression.Moreover,it also had a dose-dependent down regulate effect on nitric oxide synthesis.Conclusion This study demonstrates that HA can down-regulate the TIMP-1/MMPs expression by inhibiting NO production.

BACKGROUND:Curcumin is proved to possess anti-inflammatory, antioxidant and anti-apoptotic roles. OBJECTIVE: To summarize and discuss the mechanisms and effects of curcumin used for the treatment of osteoarthritis METHODS: PubMed, Embase, Elseveir databases were retrieved for relevant articles published from 1973 to 2014, with the key words of “osteoarthritis, curcumin, chondrocyte, articular cartilage” in English. After the quality of the included studies was evaluated, valid data were extracted and analyzed. RESULTS AND CONCLUSION:Curcumin prevents the occurrence and development osteoarthritis by inhibiting oxidative enzyme and scavenging free radicals. Curcumin increases production of colagen type 2 through suppressing the reduction of cartilage matrix by matrix metaloproteinases. Anti-inflammatory reaction of curcumin can be achieved by inhibiting cytosolic phospholipase A2, cyclooxygenase-2, and 5-lipoxygenase. Curcumin inhibits interleukin-1β-induced apoptosis of chondrocytes and mitochondrial sweling. Results from clinical trials suggest that curcumin or its derivatives can reduce joint pain and improve the function of knee joints in patients with osteoarthritis. High-concentration curcuminin vitro have been demonstrated toxic effects.

Objective To investigate the effect of hyperbaric oxygen (HBO) on expression of GAP 43 in the rats spinal cord following spinal cord injury (SCI). Methods Fifty five Wistar rats were randomly divided into three groups: a normal group, a control group and a HBO treatment group. The rats in the control and HBO treatment group underwent SCI by 10g?5cm impact at the T10 level. The rats in the HBO treatment group were treated by HBO. Western blot was used to observe the change of expression of GAP 43. Results There was weak expression of GAP 43 in the normal spinal cord. The GAP 43 expression increased significantly following SCI. The level of GAP 43 expression in the HBO treatment group was significantly high than that in the control group. Conclusion HBO can increase the level of GAP 43 expression.

Ovarian pregnancy is a rare ectopic pregnancy.In recent years,the development and popularization of assisted reproductive technology increase the incidence of ovarian pregnancy.This article focused on the ultrasound features of ovarian pregnancy following in vitro fertilization and embryo transfer (IVF-ET) by comparing those of pregnant corpus luteum and tubal pregnancy,and analyzed the advantages of transabdominal ultrasonography (TAS) and transvaginal ultrasonography (TVS) in the diagnosis.

Objective:To measure the expression of the MCP-1(Monocyte Chemoattractant Protein-1) in the serum of patients with acute spinal cord injury and explore the possible mechanism of secondary spinal cord injury.Methods:MCP-1 in the serum of patients with acute spinal cord injury,single spine compression and healthy subjects were detected by ELISA irrespectively.The MRI data of these patients were studied at the same time on a blind base.Results:MCP-1 in the serum of patients with acute spinal cord injury was correlated positively with the degree of spinal cord compression,which was elevated markedly(P

37 ℃, cell growth became poor, and clone formation decreased. At 39 ℃, cells could not adhere. Under incubator CO2 concentration between 3% and 8%, there was no significant difference in cell clone formation. CONCLUSION: The optimized culture condition of Schwann cells were 7.2 pH, 10% fetal bovine serum, 37 ℃ of temperature and 5% of CO2(v/v) of incubator.

Objective To evaluate the clinical value and relating problems in treating bilateral tibial fracture by using interlocking intramedullary nail and/or AO external fixator.MethodTotally 33 patients of bilateral tibial fracture treated with interlocking intramedullary nail and/or AO external fixator from April 1998 to January 2006 were retrospectively reviewed.There were 26 males,7 females,with an average age of 34.4 years.According to the AO/ASIF classification,there were type A 9 limbs,type B 13 limbs and type C 17 limbs.According to Gustilo classification there were type Ⅰ 10 limbs,type Ⅱ 6,ⅢA 5,type ⅢB 4 and type ⅢC 2.Duration from injury to operation was 5.5 days(3 hour～12 days). ResultAll patients were followed up for 6～36 months,with the average time being 16 months.All fractures had bon union.The union time averaged 16 weeks in closed fractures and 18 weeks in open fractures.Delayed union occurred in 4 limbs.There were 3 limbs of superficial infection.The results were excellent in 19 cases,good 11 cases,fair 3 cases according to the criteria of Johner-Wruh.Rate of excellent and good result was 90.9%.No fat embolism syndrome,deep in fection,failure of fixation and malunion were not found in this group.ConclusionThe advantages of interlocking intramedullary nail and/or AO external fixator in treatment of bilateral tibial fracture has advantages of easy performance,reduced destruction of blood supply to the fracture ends,rigid fixation,high union ratio of fractures,promotion of early functional extercise and satisfactory effect.The selection of the fixation device should be adopted according to the types of the fracture.

[Objective] To investigate the effects of pyrroloquinoline quinine(PQQ)on proliferation and expression of NF-?B of Schwann cells.[Methods]Schwann cells were cultured from sciatic nerves of SD rats in vitro.The Schwann cells were identified and purified by immunofluorescence of S-100 and Ara-C.Experimental group with was 100 nmol/L of PQQ and control group were set up.The expression of NF-?B in Schwann cells was determined by RT-PCR and Western blotting.[Results]The expression of NF-?B were up-ragulated by PQQ in cultured Schwann cells(P

[Objective]To investigate the effects of Wnt/?-catenin signal pathway on Schwann cells proliferation promoted by pyrroloquinoline quinine (PQQ) and its molecular mechanisms.[Method]Schwann cells were cultured and purified in vitro. The purity was identified by S-100. PQQ of 10 nmol/L and 100 nmol/L were added into culture medium for 24 hours,respectively. Then the morphological changes promoted by PQQ were observed by inverted microscope. The expression of ?-catenin was detected by RT-PCR and Western blot in Schwann cells promoted by PQQ of different concentration for 72 hours.[Result]Morphological change was observed in Schwann cells treated by PQQ of 10 nmol/L and 100 nmol/L. The most obvious morphological changes took place in the Schwann cells treated by 100 nmol/L of PQQ,the RT-PCR and Western blot results showed that PQQ of 1-1000 nmol/L could up-regulate the expression of ?-catenin,especially when Schwann cells was treated by PQQ of 100 nmol/L(P