Objective To investigate the psychological condition of the rural residents with HIV/AIDS.Methods Totally 192 subjects with HIV/AIDS residing in countryside of Henan Province(China)were investigated with questionaire and Symptom Check list 90(SCL-90).Results SCL-90 total score,the number of positive items and the score of each factor for the 192 subjects with HIV/AIDS were higher than those of Chinese normal(P

Objective To study the effects of remifentanil with propofol and no positive pressure ventilation induction of anesthesia in 5 minutes-cesarean section on parturients and neonates. Methods Sixty pregnant women were randomly divided into ketamine anesthesia group (group L) and remifentanil anesthesia group (group R).In group L,anaesthesia was induced with ketamin 0.5 mg/kg+propofol 2 mg/kg and group R with remifentanil 1 μg/kg+propofol 2 mg/kg.Blood pres-sure,heart rate,oxygen saturation and pulse rate of maternal were recorded at intubation (T1 ),inci-sion (T2 ),and cutting the umbilical cord (T3 ).The infant Apgar scores at 1 minute and 5 minute af-ter birth,the delivery time of the fetus and umbilical blood gas values were recorded.Results The systolic blood pressure (SBP)and heart rate (HR)were significantly higher at T1 and T2 in group L than those in group R (P <0.05).The SBP,DBP and HR had not significant difference at T3 .The delivery time of the fetus and the infant Apgar scores at 1 minute and 5 minute between the two groups had no statistically significant differences.Conclusion Remifentanil combined with propofol and without positive pressure ventilation induction of anesthesia can be safely used in 5 minutes-cesar-ean section.The maternal hemodynamics is more stable without increasing the risk of neonatal respira-tory depression.

Objective To evaluate the effect of mind mapping on adverse effects caused by medication via patient control alagesia pump(PCA).Methods One hundred and seventy-nine patients with PCA pump after operation were divided into control group(n=90)and observation group(n=89)according to the admission time.The observation group was nursed with mind mapping and the control group with routine method. The incidences of adverse effects caused by medication via PCA pump were compared between the two groups.Result The incidences of adverse effect in the observation group and the control group were 19.1%and 32.2%respectively,with statistical significance(P<0.05).Conclusion Nursing with mind mapping for medication via PAC pump can improve nurses’clinical ability in observation and nursing and therefore effectively prevent and deal with adverse effect caused by medication via PCA pump to relieve patients’pain and improve nursing quality.

Objectives To develop a rapid genotyping method of clinical isolates of Malassezia from patients with tinea versicolor by PCR-RFLP,and to evaluate reliability of the approach as compared with biochemical classification.Methods Tween assimilation test and catalase reaction were carried out to identify 74 isolates of Malassezia species from patients with tinea versicolor and 7 Malassezia reference strains.The sequence of 28S rDNA of Malassezia species was amplified by PCR,and then the product was analyzed by RFLP with Eco88I,Bsp143Ⅱ and BshNⅠ,respectively.Results M.restricta,M.obtusa and M.pachydermatis were successfully identified by three restriction endonucleases.M.restricta was found to be more diverse from the other 6 species in genetic homology.By comparison with PCR-RFLP technique,a possible mistake was discovered with biochemical method.Conclusion PCR-RFLP is a promising molecular biological technique,which could rapidly and correctly classify Malassezia species.

Objective: To investigate the effect of phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta (PIK3C2B) gene silencing on proliferation and invasion of human prostate cancer PC-3 cells in vitro, and to explore its possible mechanism. Methods: PIK3C2B-siRNAs including PIK3C2B-siRNA1 and PIK3C2B-siRNA2 and the negative control (NC)-siRNA were designed and synthesized, then transfected into PC-3 cells by LipofectAMINE 2000. The expression levels of PIK3C2B mRNA and protein were detected by real-time fluorescent quantitative-PCR and Western blotting, respectively. The proliferation and invasion abilities of PC-3 cells after transfection with PIK3C2B-siRNA were detected by MTT method and Transwell cell invasion assay, respectively. The expression levels of protein kinase B (PKB, Akt) signal pathway-related proteins Akt and phospho-Akt in PC-3 cells after transfection with PIK3C2B-siRNA were detected by Western blotting. Results: The expression levels of PIK3C2B mRNA and protein in PC-3 cells after transfection with PIK3C2B-siRNA were down-regulated. The inhibition effect of PIK3C2B-siRNA2 was stronger (P < 0.01). The proliferation abilities of PC-3 cells at 48 and 72 h after transfection with PIK3C2B-siRNA2 were significantly inhibited as compared with those of the cells transfected with NC-siRNA or not transfected with any siRNAs (all P < 0.05); the invasion ability of PC-3 cells was also decreased after transfection with PIK3C2B-siRNA2 (all P < 0.05). The expression level of Akt protein in PC-3 cells transfected with PIK3C2B-siRNA2 had no significant change, but the expression level of phospho-Akt protein was down-regulated (P < 0.05). Conclusion: PIK3C2B may promote the proliferation and invasion of prostate cancer PC-3 cells in vitro by activating Akt signaling pathway.

Objective:To study the safety and feasibility of laparoscopic surgery for treatment of hepaticojejunostomy strictures after cholangiectasis surgery in children.Methods:The clinical data of 12 children was retrospectively analysed. There were 5 males and 7 females, aged 4 (range 0.45 to 9.00) years old, who developed hepaticojejunostomy strictures after cholangiectasis surgery and underwent reoperative treatment at Anhui Provincial Children's Hospital from January 2013 to January 2021. These patients were divided into the laparoscopic surgery group ( n=5) and the open surgery group ( n=7) based on the mode of reoperation. The children were followed-up by outpatient review and the relevant clinical data of the children in the 2 groups was analyzed. Results:The reoperations were completed successfully in the 2 groups. The maximum preoperative dilated common hepatic duct diameter was significantly larger in the laparoscopic group (1.26±0.23) cm than the open group (0.64±0.19) cm ( P<0.05). The alkaline phosphatase and glutamyltransferase levels in the laparoscopic surgery group were significantly lower before the operation (all P<0.05), and the total bilirubin, direct bilirubin, alkaline phosphatase, and glutamyltransferase levels were significantly lower in the laparotomy group than before the operations (all P<0.05). In the laparoscopic group, the time of the reoperations, postoperative hospital stay, and blood loss were 268(117, 340) min, (9.0±2.9) d and (14.0±5.5) ml, respectively, while those in the open group were 180(150, 205) min, (9.7±3.4) d and (13.3±2.6) ml, respectively. There were no significant differences between the two groups (all P>0.05). On follow-up, all children were well except for one child who showed mild elevation levels of alanine aminotransferase and aspartate aminotransferase. Conclusion:Laparoscopic surgery for hepaticojejunostomy strictures after cholangiectasis surgery in children was safe and feasible. Its curative effect was no less than that of open surgery.

Objective To investigate the association of cylomegalovirus with atherosclerosis.Methods The presence of cylomegalovirus(CMV)nucleic acids was demonstrated in carotid and coronary arteries of patients with and without atherosclerosis by polymerase chain reaction(PCR).CMV was detected by PCR in the blood of patients with and without atherosclerosis.Results 83.3% - 86.7% of the samples obtained from atherosclerotic patient arterial walls were shown to contain viral nucleic acids(CMV),CMV could be found among 6.7% of patients' arterial wall without atherosclerosis,significant difference can be found between them(P ＜ 0.01).In blood CMV could be found in 42.4% of patients with atherosclerosis,in the control group,only 3% of samples had CMV,P＜0.01.Conclusion CMV plays an important role in the pathologic process of the atherosclerosis and the atherosclerotic cerebral infarction.

OBJECTIVE： To investigate the protective effect and its mechanism of thymoquinone （TQ） on myocardial fibrosis （MF） induced by lipopolysaccharide in rats. METHODS： Totally 40 male Wistar rats were randomly divided into control group， model group， TQ low-dose and high-dose groups [5， 10 mg/（kg?d）]， with 10 rats in each group. Except that control group was given constant volume of normal saline intraperitoneally， other groups were given LPS intraperitoneally to induce MF model， once a day， for consecutive 3 weeks. Since the first day of modeling， administration group was given relevant medicine intraperitoneally， once a day， for consecutive 3 weeks. After last medication， ELISA method was used to detect the contents of serum inflammation factors （IL-1β， IL-6， TNF-α） in rats. Cardiac mass parameters （HW/BM， LVW/BW） were weighed and calculated. HE staining was used to observe the histopathological changes of myocardial tissue. The contents or activities of oxidative stress indicators （MDA， SOD） and myocardial collagen indexes （HYP， Col-Ⅰ， Col-Ⅲ） were detected by chemical analysis， xanthine oxidase method or ELISA. mRNA expression of regulation genes （Col-Ⅰ， Col-Ⅲ， MMP-3， MMP-9， TGF-β1 and Smad3） related to myocardial fibrosis were determined by real-time fluorescence quantitative PCR. RESULTS： Compared with control group， there were swollen myocardial cells， disordered nuclei of different sizes and visible fiber hyperplasia in model group； the levels of serum inflammatory factors and LVW/BW， cardiac contents of MDA， HYP， Col-Ⅰand Col-Ⅲ， mRNA expression of Col-Ⅲ， TGF-β1 and Smad3 in model group were increased significantly （P＜0.05）， while SOD activity was decreased significantly （P＜0.05）. Compared with model group， the degree of myocardial fibrosis was improved in TQ groups to different extents； serum content of IL-1β and the contents of MDA， HYP and Col-Ⅰ in cardiac tissue in TQ low-dose group， serum contents of IL-1β， IL-6 and TNF-α， LVW/BW and the contents of MDA， HYP， Col-Ⅰ and Col-Ⅲ in cardiac tissue of TQ high-dose group as well as mRNA expression of Col-Ⅰ， Col -Ⅲ， TGF-β1 in TQ groups were decreased significantly （P＜0.05）. The activities of SOD in cardiac tissue were increased significantly in TQ groups （P＜0.05）. There was no statistical significance in other indexes among groups （P＞0.05）. CONCLUSIONS： TQ can protect against MF model rats to certain extent， the mechanism of which may be associated with the inhibition of inflammation reaction and oxidant stress reaction， and down-regulation of mRNA expression of Col-Ⅰ， Col-Ⅲ and TGF-β1.

Objective:To investigate the association between cytokines and ocular chronic graft-versus-host disease (cGVHD) and identify specific biomarkers for ocular cGVHD to enhance clinical diagnosis, treatment, and evaluation.Methods:A mouse model of cGVHD was established to explore the correlation between cGVHD and serum cytokines. Based on the findings from the animal experiments and literature review, a panel of 16 cytokine combinations was identified. Enzyme-linked immunosorbent assay (ELISA) was used to compare the cytokine concentrations in the serum and tear samples from patients who underwent allogeneic hematopoietic stem cell transplantation from June 2017 to March 2022 at the Medical Center of Hematology, Xinqiao Hospital, Army Medical University.Results:① Compared with the control group, mice with cGVHD exhibited elevated serum IL-1β, IL-6, IL-8, IL-17, IFN-γ, CX3CL1, CXCL11, CXCL13, CCL11, and CCL19 concentrations (all P<0.05). ② Analysis of the cytokine profiles of the serum and tear samples revealed that compared with patients without ocular cGVHD, those with ocular cGVHD exhibited increased serum IL-8 [ P=0.032, area under the curve (AUC) =0.678]; decreased serum IL-10 ( P=0.030, AUC=0.701) ; elevated IL-8, IFN-γ, CXCL9, and CCL17 in tear samples; and lower IL-10 and CCL19 in tear samples (all P<0.05, all AUC>0.7). Moreover, cytokines in tear samples showed correlations with ocular surface parameters related to ocular cGVHD. Conclusions:Tear fluid demonstrates greater specificity and sensitivity as a biomarker for diagnosing ocular cGVHD than serum biomarkers. Among the identified cytokines in tear samples, IL-8, IL-10, IFN-γ, CXCL9, CCL17, and CCL19 serve as diagnostic biomarkers for ocular cGVHD post-transplantation, offering practical reference value for diagnosis.

Acute lung injury (ALI) is a common clinical critical respiratory disease. At present, the mechanism of the disease has not been fully elucidated, there is a lack of specific drugs in clinical practice and the mortality rate is high, which is a difficult problem in the medical field. In recent years, traditional Chinese medicine has exerted its unique advantages and efficacy in the prevention and treatment of ALI, which has aroused the attention of domestic and foreign scholars. Based on the theory of "Wei Qi Ying Xue", this paper discusses the current research status of prevention and treatment of ALI by traditional Chinese medicine, and analyzes its pathogenesis, clinical manifestations and corresponding analysis with TCM syndrome. According to the angle of "Wei Qi Ying Xue", the progress of syndrome differentiation and treatment is highly consistent with immune response, inflammatory response, oxidative stress and apoptosis, in order to find new ideas and medication for the prevention and treatment of ALI with integrated traditional Chinese and Western medicine.