1.Stochastic Initiation and Propagation of Intracellular Ca~(2+) Wave in Cardiac Myocytes
Progress in Biochemistry and Biophysics 2006;0(08):-
The Ca2+ wave is a chain reaction of intracellular Ca2+ release channels through a Ca2+-induced Ca2+ release mechanism. In cardiac myocytes, Ca2+ wave has drawn much attention because it is found to induce arrhythmia genesis. To investigate the microscopic process of wave propagation, Ca2+ imaging was performed with high spatial and temporal resolution via a laser-scanning confocal microscope combined with loose-seal patch clamp. These observation and analysis revealed that Ca2+ waves originated from a stochastic recruiting of Ca2+ release units (CRUs) by a pioneer Ca2+ spark, which had a low possibility in normal cells. During wave propagation, the 'waiting' time that the wave propagate between two neighboring CRUs along propagation direction distributed normally, and cells with a lower speed had a more dispersive distribution of 'waiting' time. To study the cause of the randomicity, the wave propagation was simulated with a numerical model. The simulation showed that the intrinsic stochastic open process of CRUs can fully explain the above phenomenon. Increasing the maximal open probability of CRUs reduced the randomness of wavefront propagation and enhanced the average velocity of wave meantime. These experimental and numerical results provided an unequivocal quantification for the stochastic behavior of wave initiation and propagation.
2.Expression of annexin A1 in tissues of colorectal carcinoma and its clinical pathological significance
Chongqing Medicine 2014;(34):4608-4609,4612
Objective To investigate the expression of Annexin A1 proteins in colorectal cancer tissues and the relationship be-tween it and clinicopathologic features of colorectal cancer.Methods The surgical specimens from 48 cases of colorectal cancer,36 cases of adjacent tissues,15 cases of adenoma polyp were used to be examined expression of Annexin A1 immunohistochemical method.Results The positive expression rate of Annexin A1 in cancer tissue(64.6%)was significantly higher than those in adja-cent tissues(38.8%)and adenoma polyp tissues(40.0%)(P<0.05).The adjacent tissues and adenoma polyp tissues with positive Annexin A1 expression showed mild to severe atypical hyperplasia.The positive rates of Annexin A1 were significantly lower in the well-differentiated adenocarcinoma,tumor size of <5 cm,with no metastasis to lymphnodes and no invasion to surrounding tissues of colorectal cancer than those in the moderately or poorly differentiated adenocarcinoma,tumor size of ≥5 cm,with metastasis to lymphnodes and invasion in surrounding tissues(P<0.05).Conclusion The expression levels of Annexin A1 may be an important index of occurrence,progression and biological behaviors of colorectal carcinoma.
3.Research progress in exosomes in tissue fibrosis
Zhijian RAO ; Yun CHANG ; Shiqiang WANG
Chinese Journal of Pharmacology and Toxicology 2017;31(3):262-268
Exosomes are extracellular nanovesicles secreted by a variety of cell types such as cardiomyocyte, hepatocytes, and stem cells. They carry specific sets of mRNA, microRNA, and proteins, which play a role in intercellular communication in almost each physiological and pathological process. Exosomes, which are released after tissue cell injury, can initiate repair/regeneration responses by triggering inflammation and active fibroblast, and finally lead to tissue fibrosis. However, exosomes released by stem cells can retard tissue fibrosis by enhancing cell survival and reducing apoptosis. In this paper, we reviewed the research progress in the relationship between exosomes and tissue fibrosis.
4.Effects of Polysaccharide Sulfate on plasma von Willebrand factor and thrombomodulin in patients with acute cerebral infarction
Yongjiu WANG ; Kun LIU ; Shiqiang BI
Journal of Clinical Neurology 1988;0(02):-
Objective To investigate the effects of Polysaccharide Sulfate (PSS) on plasma von Willebrand factor (vWF) and thrombomodulin (TM) in patients with acute cerebral infarction.Methods 64 patients with acute cerebral infarction were randomly assigned to receive intravenous drip of PSS (150 mg/d for 14 days, 32 cases, PSS group) or Troxerutin (600 mg/d for 14 days, 32 cases, control group). All of the patients were tested for plasmatic levels of vWF and TM prior to and at 7 d, 14 d after treatment.Results The levels of vWF in PSS group at 7 d, 14 d after treatment were obviously decreased compared with control group (all P
5.Effects of MiR-21 on Exercises-induced Right Ventricularfibrosis
Zhijian RAO ; Yun CHANG ; Shiqiang WANG
Chinese Journal of Sports Medicine 2017;36(7):571-577
Objective To explore the expression level of miR-21 in exercise-induced right ventricular (RV) fibrosis,and to analyze the role of miR-21 in exercise-induced right ventricular fibrosis.Methods Seventy-two male Sprague-Dawley rats were randomly divided into a sedentary (Sed) group,a moderate exercise (ME) group and an intensive exercise (IE) group,each of 24.Rats in the Sed group were free of exercises,while those in ME and IE groups ran an hour on treadmill at 5°and 10° slopes at the speed of 15.2 m/min and 28 m/min respectively for 8 weeks,12 weeks or 16 weeks every day,5days per week.Twenty-four hours after the last training,all rats were sacrificed after blood sampling.The right ventricles were removed,and the collagen volume fraction (CVF) was tested using Sirius red staining,Collagen Ⅰ (Col Ⅰ) content was quantified using Immunofluorescence.The expression level of miR-21 was measured using the reverse transcription-polymerase chain reaction (RT-PCR).Results Af ter 12 weeks (P<0.05,P<0.05) and 16 weeks (P<0.01,P<0.01) of intensive exercises,the average CVF in the right ventricular was significantly higher than that of Sed and ME rats.Compared to Sed and ME groups,12 weeks (P<0.01,P<0.01) and 16 weeks (P<0.01,P<0.01) of intensive exercises significantly increased RV collagen Ⅰ content.Compared to the Sed group,the expression of miR-21 in RV increased significantly in the IE group (P<0.01,P<0.05 and P<0.05).After 16-week intensive exercises,the miR 21 expression was positively correlated with the RV Col Ⅰ content.Conclusion The right ventricular fibrosis induced by long-term intensive exercises is associated with increased miR-21 expression level.Therefore,miR-21 is a potent therapeutic target and novel biomarker of the exercise-induced right ventricular fibrosis.
6.Study on the treatment of children dependent on network game of psychological intervention combined with drug
Dongping WANG ; Shiqiang WEI ; Heqin ZHAN
Chinese Journal of Applied Clinical Pediatrics 2014;29(15):1170-1173
Objective To study the curative effect of psychological intervention combined with drug treatment of children addicted to the network game.Methods One hundred and twelve children addicted to the network game were selected,who were admitted to the outpatient department of the Second Affiliated Hospital of Xinxiang Medical University from Feb.2009 to Oct.2013 were selected,according to the admission time sequence,they were randomly divided into drug treatment group,psychological intervention group(as control group) and psychological intervention combined with drug therapy group(test group).Each of the control groups had 38 cases of patients,while treatment group had 36 cases.Drug treatment group was treated with oral Fluoxetine Hydrochloride Capsules 10 mg,1 time/d; the psychological intervention group was given the general psychological conversation,until discharged from the hospital; the test group adopted the cognitive restructuring,imagine the debate,self suggestion,remind yourself of cognitive therapy and behavior contract,behavior reinforcement,behavior extinction,aversive stimulus,self-regulation of behavior therapy combined with drug therapy.Respectively,before treatment,2 weeks,4 weeks and 8 weeks after the end of the treatment,Chinese Internet Addiction Scale,Coping Style Questionnaire (CSQ) and symptom checklist 90 (SCL-90) of each factor were evaluated.Results By the end of the 2 weeks and 8 weeks after the treatment,CIAS scores in the treatment group were significantly lower than that in drug therapy and psychological intervention group,the differences were statistically significant (t =2.49,2.03,all P < 0.05 ; t =2.72,2.72,all P < 0.01) ; scores of 3 factors of fantasy,self accusation and retreat in CSQ group in coping style questiennaire in the coping style questionnaire in the treatment group were lower than those in control groups,the differences were statistically significant (t =3.99,3.78,2.15,all P < 0.05; t=2.64,4.20,3.72,all P < 0.01); the problem solving,help seeking factor in the test group were higher than those in the control groups,the differences were statistically significant (t =2.69,2.43,5.31,2.25; all P < 0.01) ; variation test combined physicochemical factors scores at different stages had on difference comparison of those in the 2 control groups (all P > 0.05).Comparison of the SCL-90 score between the test group and the 2 control groups,the differences were statistically significant (t =5.05,4.31,all P < 0.01).There was no significant difference of the scores between the 2 control groups (all P > 0.05).Conclusions Psycholoical intervention therapy including cognitive therapy,behavior therapy combined with drug treatment have good effect on children's addiction to Web games.
7.Current problems and prospect on tandem mass spectrometry based newborn inherited metabolic diseases screening
Shiqiang SHANG ; Jianbin YANG ; Zhiguo WANG
Chinese Journal of Laboratory Medicine 2016;39(4):237-239
Newborn screening of inherited metabolic diseases by tandem mass spectrometry is flourishing in our country.With expansion of the coverage and spectrum of diseases, it is important to strengthen the quality management, optimize the performance and reduce the false rate currently, such as quality assurance of the specimens, quality control of the process, quality verification of the test procedure, quality evaluation of the laboratory and quality optimization of the interpretation method. Along with development of the equipment, software and project, promotion of regional collaboration by data and experience sharing will be more critical in the future, and national neonatal screening by tandem mass spectrometry will step into a new stage.
8.Clinical research on systemic chemotherapy concurrent whole brain radiotherapy for brain metastases from non-small cell lung cancer
Xiang WANG ; Shiqiang ZHANG ; Youwei ZHANG
Journal of International Oncology 2015;42(2):81-83
Objective To explore the feasibility and safety of systemic chemotherapy concurrent whole brain radiotherapy (WBRT) for non-small cell lung cancer (NSCLC) with brain metastases.Methods Eighty cases of NSCLC patients with brain metastases were divided into observation group (40 cases) and the control group (40 cases) according to random number table.Patients were given systemic chemotherapy synchronous WBRT or sequential WBRT.Results The Ⅰ-Ⅳ degree leukocytopenia incidences of the two groups were 12.5%,25.0%,25.0%,0.0 and 30.0%,25.0%,12.5%,15.0%,and there was statistical significance (x2 =12.12,P < 0.05).In the observation group,the total remission rate was 20% (8/40),and it was 22.5% (9/40) in the control group,with no significant difference (x2 =1.79,P > 0.05).The median progression free survival of the observation group and of the control group were (3.5 ± 2.3) months and (3.6 ±1.1) months,respec-tively,with no significant difference (t =5.23,P > 0.05).But the 1-years survival rate in the observation group was 37.5% (15/40),significantly higher than that in the control group (17.5%,7/40),which had statistical significance (x2 =9.11,P < 0.05).Conclusion The safety of systemic chemotherapy synchronous WBRT for NSCLC patients with brain metastases is higher,with good curative effect and strong application feasibility.
9.Study on HPLC fingerprint of Pollen Typhae
Ying YANG ; Shiqiang WANG ; Guangxian CAI
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(05):-
bjective:To study and establish the fingerprint of Pollen Typhae.Methods:Hypersil BDS C18(5?m,4.6mm?250mm) chromatographic column mobile phase acetonitrile-0.1 % phosphoric acid solution(10:90)with fiow rate of 1.0 ml/min and UV detector at 254 nm.ResultsTaking Isorhamnetin-3-O-neohesperidoside as the reference peak,11 common peaks were selected as the fingerprint peaks of Pollen Typhae,Technology investigation indicated that the analytical method this study established has desirable precision,reproducibility,and stability.The similarity of Pollen Typhae fingerprints from different batches is better.Conclusion HPLC fingerprint analysis can be a method for quality control of medical material of Pollen Typhae.
10.Single nucleotide polymorphisms and haplotypes of MBL gene in Han and Hui ethnic children of Han and Hui ethnics
Hong WANG ; Chunzhen HUA ; Shiqiang SHANG
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
Objective To analyze the single nucleotide polymorphisms(SNPs) in promoter region,5' untranslated region,exon 1 and haplotypes of mannose binding-lectin(MBL) gene in Han nationality and Hui etheic children in China.Methods Sixty nine Hui ethnic children from Ningxia Huizu Auto.Reg.and 105 Han children from Zhejiang Prov.were enrolled in the present study.Whole blood samples(1.5 ml) were collected into potassium-EDTA tubes.SNPs in promoter region,5' untranslated region and exon 1 of MBL gene were determined by sequence analysis using BigDye Mix 3730 genetic analyzer,and genetic analysis was performed using SHEsis software.Results The variant allele frequencies at-221 sites in Han and Hui objects were 0.091 and 0.123,respectively,with no difference between the two groups(?2=0.684,P=0.408).No mutation was found at sites +223 and +239 in exon 1 of MBL gene in the study.The variant frequency at +230 site in Hui children(0.268) was significantly higher than that in Han objects(0.167,?2=5.223,P=0.022).The most common haplotype was YA,and the frequencies of YA haplotype in Han and Hui ethnic were 0.770 and 0.669,respectively,with significant difference between the two groups(?2=4.312,P=0.038).Conclusion The variant allele frequency at +230 sites in exon 1 in Hui ethnic children is higher than that in Han subjects.The most common haplotype is YA,and the frequency of YA haplotype is higher in Han children than that in Hui subjects.