Objective To investigate the expression of Annexin A1 proteins in colorectal cancer tissues and the relationship be-tween it and clinicopathologic features of colorectal cancer.Methods The surgical specimens from 48 cases of colorectal cancer,36 cases of adjacent tissues,15 cases of adenoma polyp were used to be examined expression of Annexin A1 immunohistochemical method.Results The positive expression rate of Annexin A1 in cancer tissue(64.6%)was significantly higher than those in adja-cent tissues(38.8%)and adenoma polyp tissues(40.0%)(P<0.05).The adjacent tissues and adenoma polyp tissues with positive Annexin A1 expression showed mild to severe atypical hyperplasia.The positive rates of Annexin A1 were significantly lower in the well-differentiated adenocarcinoma,tumor size of <5 cm,with no metastasis to lymphnodes and no invasion to surrounding tissues of colorectal cancer than those in the moderately or poorly differentiated adenocarcinoma,tumor size of ≥5 cm,with metastasis to lymphnodes and invasion in surrounding tissues(P<0.05).Conclusion The expression levels of Annexin A1 may be an important index of occurrence,progression and biological behaviors of colorectal carcinoma.

The Ca2+ wave is a chain reaction of intracellular Ca2+ release channels through a Ca2+-induced Ca2+ release mechanism. In cardiac myocytes, Ca2+ wave has drawn much attention because it is found to induce arrhythmia genesis. To investigate the microscopic process of wave propagation, Ca2+ imaging was performed with high spatial and temporal resolution via a laser-scanning confocal microscope combined with loose-seal patch clamp. These observation and analysis revealed that Ca2+ waves originated from a stochastic recruiting of Ca2+ release units (CRUs) by a pioneer Ca2+ spark, which had a low possibility in normal cells. During wave propagation, the 'waiting' time that the wave propagate between two neighboring CRUs along propagation direction distributed normally, and cells with a lower speed had a more dispersive distribution of 'waiting' time. To study the cause of the randomicity, the wave propagation was simulated with a numerical model. The simulation showed that the intrinsic stochastic open process of CRUs can fully explain the above phenomenon. Increasing the maximal open probability of CRUs reduced the randomness of wavefront propagation and enhanced the average velocity of wave meantime. These experimental and numerical results provided an unequivocal quantification for the stochastic behavior of wave initiation and propagation.

Renal cell carcinoma (RCC) is one of the most lethal malignancies of the urinary system, however, its pathogenic mechanism is not clear.Stanniocalcin (STC) is a type of glycoprotein hormone with multiple biological functions.Recently, the role of STC in the pathogenesis of cancer is of intriguing interest, and many researches have been performed to clarify underlying mechanism. We emphasized the role of STC in the underlying mechanism of RCC progression, from the aspects of STC inducing hypoxia adaptation of tumor cells, promoting tumor angiogenesis, also promoting cell proliferation, apoptosis, invasion and metastasis, and inhibiting the immune response.Conclusively, STC could be used as both a promising biomarker for RCC diagnosis and a theraputic target of renal cell carcinoma.

Exosomes are extracellular nanovesicles secreted by a variety of cell types such as cardiomyocyte, hepatocytes, and stem cells. They carry specific sets of mRNA, microRNA, and proteins, which play a role in intercellular communication in almost each physiological and pathological process. Exosomes, which are released after tissue cell injury, can initiate repair/regeneration responses by triggering inflammation and active fibroblast, and finally lead to tissue fibrosis. However, exosomes released by stem cells can retard tissue fibrosis by enhancing cell survival and reducing apoptosis. In this paper, we reviewed the research progress in the relationship between exosomes and tissue fibrosis.

Objective To explore a rapid diagnostic method in neonatal sepsis and bacterial me- ningitis. Methods The primers and TaqMan probes were designed and synthesized based on the sequences of bacterial 16S rRNA gene. Nineteen bacterial strains, 3 different viruses, fungus and human genomic DNA were tested by FQ-PCR assay. Blood specimens and CSF from 195 cases of suspected septicemia were detected with both TaqMan PCR assay and blood or/and CSF culture. Results The FQ-PCR showed very high sensitivity and specificity and was able to detect at least 10 copies of 16S rRNA gene equivalent to 1~2 copies bacterium. No cross-reaction was found with human genomic DNA, other fungus and viruses. Among the 195 cases, the positive rate by FQ-PCR was 12.8%(25 cases) and 7.1%(15 cases) by blood culture ( P

[Objective]To evaluate the clinical results of posterior hemivertebra resection and correction with transpedicular instrumentation for congenital scoliosis.[Method]From July 2002 to December 2006,thirty-nine consecutive cases,including 22 males and 17 females,underwent posterior hemivertebra resection and correction using transpedicular instrumentation.The average age at surgery was 8.3 years ranging from 3 to 15 years.There were 30 cases with fully segmented hemivertebra and 9 cases with partial segmented hemivertebra.Instrument included 16 cases of ISOLA,10 cases of CD-Horizon,10 cases of Moss-Miami and 3 cases of TSRH.The pre-and post-operative standing radiographic and at final follow-up were measured,and the Cobb's angles in the coronal and sagittal plane were analyzed,respectively.[Result]The average follow-up was 2.8 years ranging from 1 to 4.5 years.The Cobb's angle in coronal plane was corrected from 48? to 13?postoperatively and 13.2? at final follow-up,representing 70.2% correction rate.The kyphosis was corrected from 39? to 12.1? postoperatively and 12.7? at final follow-up,representing 68.7% correction rate.All cases had bony fusion,there was no neurological complication,no implant failure or infection.One case had crankshaft phenomenon.[Conclusion]Posterior hemiveterbra resection with instrumentation is a safe and effective treatment option for congenital scoliosis.Congenital scoliosis should be treated in early age.

bjective:To study and establish the fingerprint of Pollen Typhae.Methods:Hypersil BDS C18(5?m,4.6mm?250mm) chromatographic column mobile phase acetonitrile-0.1 % phosphoric acid solution(10:90)with fiow rate of 1.0 ml/min and UV detector at 254 nm.ResultsTaking Isorhamnetin-3-O-neohesperidoside as the reference peak,11 common peaks were selected as the fingerprint peaks of Pollen Typhae,Technology investigation indicated that the analytical method this study established has desirable precision,reproducibility,and stability.The similarity of Pollen Typhae fingerprints from different batches is better.Conclusion HPLC fingerprint analysis can be a method for quality control of medical material of Pollen Typhae.

Objective To analyze the single nucleotide polymorphisms(SNPs) in promoter region,5' untranslated region,exon 1 and haplotypes of mannose binding-lectin(MBL) gene in Han nationality and Hui etheic children in China.Methods Sixty nine Hui ethnic children from Ningxia Huizu Auto.Reg.and 105 Han children from Zhejiang Prov.were enrolled in the present study.Whole blood samples(1.5 ml) were collected into potassium-EDTA tubes.SNPs in promoter region,5' untranslated region and exon 1 of MBL gene were determined by sequence analysis using BigDye Mix 3730 genetic analyzer,and genetic analysis was performed using SHEsis software.Results The variant allele frequencies at-221 sites in Han and Hui objects were 0.091 and 0.123,respectively,with no difference between the two groups(?2=0.684,P=0.408).No mutation was found at sites +223 and +239 in exon 1 of MBL gene in the study.The variant frequency at +230 site in Hui children(0.268) was significantly higher than that in Han objects(0.167,?2=5.223,P=0.022).The most common haplotype was YA,and the frequencies of YA haplotype in Han and Hui ethnic were 0.770 and 0.669,respectively,with significant difference between the two groups(?2=4.312,P=0.038).Conclusion The variant allele frequency at +230 sites in exon 1 in Hui ethnic children is higher than that in Han subjects.The most common haplotype is YA,and the frequency of YA haplotype is higher in Han children than that in Hui subjects.

Objective To investigate the effects of Polysaccharide Sulfate (PSS) on plasma von Willebrand factor (vWF) and thrombomodulin (TM) in patients with acute cerebral infarction.Methods 64 patients with acute cerebral infarction were randomly assigned to receive intravenous drip of PSS (150 mg/d for 14 days, 32 cases, PSS group) or Troxerutin (600 mg/d for 14 days, 32 cases, control group). All of the patients were tested for plasmatic levels of vWF and TM prior to and at 7 d, 14 d after treatment.Results The levels of vWF in PSS group at 7 d, 14 d after treatment were obviously decreased compared with control group (all P

Object To study the condition of Wenpi Decoction (WPD) with being decocted first and later added medicinal materials. Methods Contents of aconitine and free anthraquinone in WPD were determined by HPCE. Results Aconitine could reach the safe limit after Radix Aconiti Preparata being decocted 30 min first and the content of free anthraquinone was the highest after Radix et Rhizomea Rhei being decocted 10 min later. Conclusion Radix Aconiti Preparata should be decocted first and Radix et Rhizomea Rhei should be decocted later in WPD.