To investigate the use of antibiotics in the patients with non-Helicobacter pylori (Hp)peptic ulcer, 487 patients of 3 hospitals were analyzed retrospectively. Our results showed that in the 487 cases, the antibiotics were used in 44.3% of the patients. Among them, 56.5 % of the patients were not indicated for the use of antibiotics. There was a significant difference between patients on Free Medical Care Program and non-free-medical care program patients(P＜0.001 ). No significant difference was found in the use of antibiotics between Class lA and Class 2A hospitals( P＞0.05 ). It is concluded that increasingly serious and extensive abuse of antibiotics exists in the treatment of peptic ulcer and steps should be held in check.