1.Successful Treatment of Fungemia Caused by Cyberlindnera fabianii with Anidulafungin: A Case Report.
Jeong In LEE ; Shinae YU ; Jong Sin PARK ; Eun Jeong JOO ; Jong Hee SHIN ; Min Jung KWON
Annals of Clinical Microbiology 2015;18(3):94-97
Cyberlindnera fabianii (previously known as Hansenula fabianii, Pichia fabianii, and Lindnera fabianii) is a yeast species that forms a biofilm, allowing it to resist azole drugs. In this study, we report a case of fungemia with C. fabianii that was successfully treated with anidulafungin. In this case, the organism was initially misidentified as Candida utilis (with a high probability of 93%, suggesting good identification) using the VITEK 2 yeast identification card (YST ID; bio-Merieux, USA). The species responsible for the patient's fungemia was correctly identified after sequencing the internally transcribed spacer region and the D1/D2 domain of the large subunit (26S) rDNA gene. The CLSI M27-A3 broth microdilution method was used to determine the in vitro antifungal activity of anidulafungin and fluconazole against C. fabianii. The MICs of anidulafungin and fluconazole were found to be 0.03 microg/mL and 2 microg/mL, respectively. The patient recovered after 14 days of anidulafungin treatment.
Biofilms
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Candida
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Danazol
;
DNA, Ribosomal
;
Fluconazole
;
Fungemia*
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Humans
;
Pichia
;
Yeasts
2.A Questionnaire Survey of HLA Crossmatch Tests in Korea (2015).
Shinae YU ; Eun Suk KANG ; Myoung Hee PARK
Laboratory Medicine Online 2017;7(3):147-156
BACKGROUND: We carried out a questionnaire survey for laboratories performing human leukocyte antigen-crossmatch (HLA-XM) to provide a basis for laboratory standardization of HLA-XM tests in Korea. METHODS: The questionnaires were distributed to 51 HLA laboratories participating in the HLA-XM part of the HLA proficiency survey program organized by the Korean Society for Laboratory Medicine and replies from 50 laboratories were analyzed. The questionnaires included following items: 1) HLA-XM methods performed and annual number of tests, 2) types of the specimen and lymphocyte separation methods, 3) test procedures and reagents for complement-dependent cytotoxicity crossmatch (CDC-XM) and flow cytometry crossmatch (FCXM). RESULTS: The number of laboratories performing anti-human globulin (AHG) CDC-XM (47/49, 96%) and FCXM (30/50, 60%) was considerably increased compared to the 2005 survey (AHG CDC-XM, 35/43, 81%; FCXM, 7/44, 16%). As for the annual number of XM tests, more than 50% of the laboratories were low volume laboratories performing ≤50 tests, and only 10% of the laboratories were performing >500 tests. For cell isolation methods, negative selection was used by 43% (21/49) of laboratories performing CDC-XM. Number of cells reacted per 1 µL of serum varied among different laboratories in both CDC-XM (1,000–8,000) and FCXM tests (1,300-20,000). For the interpretation of FCXM, log fluorescence ratio (26/30, 87%) was more commonly used than channel shift values (5/30, 17%). CONCLUSIONS: Considerable variation is noted in both CDC-XM and FCXM methods performed by different laboratories. A continuous effort for laboratory standardization is needed to reduce inter-laboratory variation in the HLA-XM test results.
Cell Separation
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Flow Cytometry
;
Fluorescence
;
Humans
;
Indicators and Reagents
;
Korea*
;
Leukocytes
;
Lymphocytes
3.Analysis of Acute Myeloid Leukemia in Korean Patients with Sole Trisomy 6.
Shinae YU ; Min Jung KWON ; Seung Tae LEE ; Hee Yeon WOO ; Hyosoon PARK ; Sun Hee KIM
Annals of Laboratory Medicine 2014;34(5):402-404
No abstract available.
Adult
;
Aged
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Aged, 80 and over
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Asian Continental Ancestry Group/*genetics
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Child
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Chromosomes, Human, Pair 6
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Female
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Humans
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Infant
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Leukemia, Myeloid, Acute/*genetics/pathology
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Male
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Middle Aged
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Republic of Korea
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*Trisomy
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Young Adult
4.Performance Evaluation of MEDITAPE UC-11A Strip Test in Estimating the Urine Albumin-to-Creatinine Ratio and Urine Protein-to-Creatinine Ratio
Shinae YU ; Sae Am SONG ; Kyung Ran JUN ; Jeong Nyeo LEE
Laboratory Medicine Online 2020;10(1):52-57
30 mg/g), the concordance rate, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of UACR, analyzed using MEDITAPE UC-11A, were 80.5, 97.5, 67.0, 70.3, and 97.1%, respectively. Using UPCR, analyzed via quantitative assay, as a reference to estimate proteinuria (UPCR >0.15 g/g), the concordance rate, sensitivity, specificity, PPV, and NPV of UPCR, analyzed using MEDITAPE UC-11A, were 86.7, 94.4, 81.5, 77.6, and 95.6%, respectively.CONCLUSIONS: UACR and UPCR, analyzed using MEDITAPE UC-11A, exhibited relatively high sensitivity and NPV, which is beneficial for laboratory screening for both albuminuria and proteinuria.]]>
Albuminuria
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Chronic Disease
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Humans
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Hypertension
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Kidney Diseases
;
Mass Screening
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Proteinuria
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Renal Insufficiency, Chronic
;
Sensitivity and Specificity
5.Comparison of Two Automated Immunoassays for the Detection of Anti-Hepatitis A Virus Total Immunoglobulin and IgM.
Sang Yong SHIN ; Hyun Jin LIM ; Changmin YI ; Shinae YU ; Min Jung KWON ; Hyosoon PARK ; Young Jae KIM ; Chae Lim JUNG ; Hee Yeon WOO
Journal of Laboratory Medicine and Quality Assurance 2011;33(2):103-109
BACKGROUND: The detection of total anti-hepatitis A virus (anti-HAV) immunoglobulin (Ig) and IgM is important for diagnosing acute hepatitis A. Our laboratory introduced new commercial automated chemiluminescence immunoassays (CLIAs) for use in addition to pre-existing automated CLIA. We evaluated the rate of agreement in the detection of total anti-HAV Ig and IgM in serum samples between two automated CLIAs. METHODS: We analyzed 181 samples those were submitted for testing at Kangbuk Samsung Medical Center. We analyzed the rate of agreement between the ADVIA Centaur XP (Siemens, Germany) and the MODULAR ANALYTICS E170 (Roche, Switzerland) analyzers. We performed reverse transcription (RT)-PCR when there was a discrepancy between the results from the two analyzers. RESULTS: The agreement rates between the ADVIA Centaur XP and the MODULAR ANALYTICS E170 for total anti-HAV Ig and IgM were 97.2% and 98.9%, respectively. Discrepant results were obtained in seven cases; all were found to be HAV-negative based on RT-PCR analysis. CONCLUSIONS: The total anti-HAV Ig and IgM results obtained using the two automated analyzers were comparable. However, in cases of equivocal results tested by the ADVIA Centaur XP for anti-HAV IgM, retesting and follow-up testing of samples are recommended.
Hepatitis A
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Hepatitis A Antibodies
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Hepatitis A virus
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Immunoassay
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Immunoglobulin M
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Immunoglobulins
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Luminescence
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Reverse Transcription
;
Viruses
6.A Rare Case of Acute Leukemic Presentation of Blastic Plasmacytoid Dendritic Cell Neoplasm without Cutaneous Lesions.
Shinae YU ; Min Jung KWON ; Kyungeun KIM ; Dong Hoe KOO ; Hee Yeon WOO ; Hyosoon PARK
Annals of Laboratory Medicine 2014;34(2):148-151
No abstract available.
Acute Disease
;
Adult
;
Antigens, CD4/metabolism
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Antigens, CD45/metabolism
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Antigens, CD56/metabolism
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Bone Marrow Cells/cytology
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Flow Cytometry
;
Hematologic Neoplasms/*diagnosis/pathology
;
Humans
;
Interleukin-3 Receptor alpha Subunit/metabolism
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Lymph Nodes/metabolism/pathology
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Male
;
Tomography, X-Ray Computed
7.Therapeutic Plasma Exchange for a Patient with Severe Cold Agglutinin Disease.
Naery YANG ; Sholhui PARK ; Hae Sun CHUNG ; Yeung Chul MUN ; Shinae YU ; Min Jung KWON ; Hee Yeon WOO ; Sukjoong OH ; Miae LEE
Korean Journal of Blood Transfusion 2015;26(1):75-80
Cold agglutinin disease is a kind of autoimmune hemolytic anemia, caused by cold agglutinin, serum autoantibodies activated at reduced body temperatures to produce red blood cell agglutination and hemolysis. In this paper we described a case of severe hemolytic anemia in a cold agglutinin disease patient treated with therapeutic plasma exchange. Therapeutic plasma exchanges were performed four times every other day. Over the same period, a total of 8 units of washed red blood cells were transfused. Then hemoglobin was increased from 4.0 g/dL to 7.8 g/dL. On the 12th hospital day hemoglobin level was decreased again to 4.2 g/dL and fludarabine chemotherapy was started on the 14th hospital day. The patient's symptoms were relieved and she was discharged on the 30th hospital day. As in this case, therapeutic plasma exchange could be considered as secondary therapy for temporary improvement of acute severe hemolytic anemia in cold agglutinin disease.
Agglutination
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Anemia, Hemolytic
;
Anemia, Hemolytic, Autoimmune*
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Autoantibodies
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Body Temperature
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Drug Therapy
;
Erythrocytes
;
Hemolysis
;
Humans
;
Plasma Exchange*
8.Bortezomib Induced Tumor Lysis Syndrome in Multiple Myeloma.
Shinae YU ; Sung Woo RYU ; Kyoung Ha KIM ; Se Hyoung KIM ; Nam Su LEE ; Sung Kyu PARK ; Jong Ho WON
Soonchunhyang Medical Science 2013;19(1):31-33
The tumor lysis syndrome (TLS) commonly occurs in the lymphoproliferative disorder, either spontaneously or in response to therapy. TLS is uncommon in multiple myeloma. However, with the use of bortezomib in the treatment of multiple myeloma, cases of TLS have been reported. We report here threepatients who presented with TLS after the administration of bortezomib. Two of them presented mild symptoms and recovered with hydration only. However, death of the other patient was associated with TLS. We should monitor patients who had high tumor burden, especially in early phase of bortezomib therapy and appropriate prophylaxis for high risk patient is also needed.
Boronic Acids
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Humans
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Lymphoproliferative Disorders
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Multiple Myeloma
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Organothiophosphorus Compounds
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Pyrazines
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Tumor Burden
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Tumor Lysis Syndrome
;
Bortezomib
9.Case Report of Kidney Paired Donation (KPD) with Desensitization: the Strategy and Experience of 3-Way KPD in Samsung Medical Center
Dongkyu OH ; Eun Suk KANG ; Shinae YU ; Kyoungsuk CHUN ; Wooseong HUH ; Hye Ryoun JANG ; Chan Woo CHO ; Nuri LEE ; Kyo Won LEE ; Hyojun PARK ; Jae Berm PARK ; Sung Joo KIM
Journal of Korean Medical Science 2018;33(5):e39-
As the need for the organ donation increases, strategies to increase kidney transplantation (KT) through expanded living donation have become essential. These include kidney paired donation (KPD) programs and desensitization in incompatible transplantations. KPD enables kidney transplant candidates with incompatible living donors to join a registry with other incompatible pairs in order to find potentially compatible living donor. Positive cross match and ABO incompatible transplantation has been successfully accomplished in selective cases with several pre-conditionings. Patients who are both difficult-to-match due to broad sensitization and hard-to-desensitize because of donor conditions can often be successfully transplanted through a combination of KPD and desensitization. According to the existing data, KPD can increase the number of KTs from living donors with excellent clinical results. This is also a cost-effective treatment as compared with dialysis and desensitization protocols. We carried out 3-way KPD transplantation with one highly sensitized, positive cross match pair and with two ABO incompatible pairs. Herein we report our first successful 3-way KPD transplantation in a single center. To maximize donor-recipient matching and minimize immunologic risk, KPD programs should use proper algorithms with desensitization to identify optimal donor with simultaneous two-, three- or more complex multi-way exchanges.
Dialysis
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Humans
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Kidney Transplantation
;
Kidney
;
Living Donors
;
Tissue and Organ Procurement
;
Tissue Donors
10.Does the Different Locations of Colon Cancer Affect the Oncologic Outcome? A Propensity-Score Matched Analysis
Kwan Mo YANG ; In Ja PARK ; Jong Lyul LEE ; Yong Sik YOON ; Chan Wook KIM ; Seok Byung LIM ; Na Young KIM ; Shinae HONG ; Chang Sik YU ; Jin Cheon KIM
Annals of Coloproctology 2019;35(1):15-23
PURPOSE: We evaluate the prognostic value of primary tumor location for oncologic outcomes in patients with colon cancer (CC). METHODS: CC patients treated with curative surgery between 2009 and 2012 were classified into 2 groups: right-sided colon cancer (RCC) and left-sided colon cancer (LCC). Recurrence-free survival (RFS) and overall survival (OS) were examined based on tumor stage. Propensity scores were created using eight variables (age, sex, T stage, N stage, histologic grade, presence of lymphovascular invasion/perineural invasion, and microsatellite instability status). RESULTS: Overall, 2,329 patients were identified. The 5-year RFSs for RCC and LCC patients were 89.7% and 88.4% (P = 0.328), respectively, and their 5-year OSs were 90.9% and 93.4% (P = 0.062). Multivariate survival analyses were carried out by using the Cox regression proportional hazard model. In the unadjusted analysis, a marginal increase in overall mortality was seen in RCC patients (hazard ratio [HR], 1.297; 95% confidence interval [CI], 0.987–1.704, P = 0.062); however, after multivariable adjustment, similar OSs were observed in those patients (HR, 1.219; 95% CI, 0.91–1.633; P = 0.183). After propensity-score matching with a total of 1,560 patients, no significant difference was identified (P = 0.183). A slightly worse OS was seen for stage III RCC patients (HR, 1.561; 95% CI, 0.967–2.522; P = 0.068) than for stage III LCC patients. The 5-year OSs for patients with stage III RCC and stage III LCC were 85.5% and 90.5%, respectively (P = 0.133). CONCLUSION: Although the results are inconclusive, tumor location tended to be associated with OS in CC patients with lymph node metastasis, but it was not related to oncologic outcome.
Colon
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Colonic Neoplasms
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Humans
;
Lymph Nodes
;
Microsatellite Instability
;
Mortality
;
Neoplasm Metastasis
;
Propensity Score
;
Proportional Hazards Models
;
Treatment Outcome